• 생명과학회지
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• 제32권2호
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• pp.86-93
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• 2022

Lupeol은 pentacyclic triterpene의 일종으로 다양한 질병에 약리 효과가 있는 것으로 보고되어 있으나, lupeol이 포도당 흡수에 미치는 영향은 아직 보고된 바 없다. 본 연구에서 3T3-L1 지방세포에서 포도당 흡수에 대한 lupeol의 효과를 조사하였다. 그 결과, Lupeol은 3T3-L1 지방세포에서 GLUT4를 원형질막으로 이동시켜 포도당 흡수를 촉진하였으며, 이는 PI3K/AKT 및 AMPK 경로의 활성화와 관련되어 있었다. PI3K/AKT 경로에서 lupeol은 PI3K를 활성화시키는 insulin receptor substrate 1의 인산화와 AKT의 인산화를 촉진하지만 비정형 단백질 키나아제 C isoforms ζ 및 λ의 인산화는 촉진하지 않았다. 반면, lupeol은 5 'AMP-activated protein kinase의 인산화를 촉진하였고, Lupeol의 의한 AMPK의 활성화는 원형질막-GLUT4의 발현과 세포내 포도당 흡수를 증가시키는 것으로 확인되었다. 3T3-L1 지방세포에서 lupeol에 의한 포도당 흡수 효과는 PI3K 억제제인 wortmannin 및 AMPK 억제제인 Compound C에 의해 억제됨을 통해 확인하였다. 본 연구 결과는 lupeol이 3T3-L1 지방세포에서 PI3K/AKT 및 AMPK 경로를 통해 원형질막 GLUT4의 발현을 자극함으로써 인슐린 감수성을 증가시켜 포도당 흡수를 촉진할 수 있음을 제시하고 있다.

• International Journal of Advanced Culture Technology
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• 제5권1호
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• pp.70-75
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• 2017

Recent development of IT industry including smart phones and communication technologies has resulted in rapid growth of Internet of Things (IoT) technology and relevant markets. The access to IoT is becoming easier thanks to the boards and IoT products, such as Arduino and Raspberry Pi. Large-scale business sites use IoT technology to manage inventories, but small-scale business sites do not. In the present study, We ported Linux-based Raspbian to Raspberry Pi, It utilizes web server communication to control the Arduino through the application We used a color sensor to figure out the kind of inventory. We also built a database using MySQL to store the data. We used raspberry pi to check whether the proposed system works and apply it to small-scale business.

• 한국작물학회지
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• 제56권4호
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• pp.329-341
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• 2011

The objective of this study was to determine the genetic diversities of major rice blast resistance genes among 84 accessions of aromatic rice germplasm. Eighty four accessions were characterized by a dominant 11 set of PCR-based SNP and CAPS marker, which showed the broad spectrum resistance and closest linkage to seven major rice blast resistance (R) genes, Pia, Pib, Pii, Pi5 (Pi3), Pita (Pita-2), and Pi9 (t). The allele specific PCR markers assay genotype of SCAR and STS markers was applied to estimate the presence or absence of PCR amplicons detected with a pair of PCR markers. One indica accession, Basmati (IT211194), showed the positive amplicons of five major rice blast resistance genes, Pia, Pi5 (Pi3), Pib, Pi-ta (Pi-ta2), and Pik-5 (Pish). Among 48 accessions of the PCR amplicons detected with yca72 marker, only five accessions were identified to Pia gene on chromosome 11. The Pib gene was estimated with the NSb marker and was detected in 65 of 84 accessions. This study showed that nine of 84 accessions contained the Pii gene and owned Pi5 (Pi3) in 42 of 84 accessions by JJ817 and JJ113-T markers, which is coclosest with Pii on chromosome 9. Only six accessions were detected two alleles of the Pita or Pita-2 genes. Three of accessions were identified as the Pi9 (t) gene locus.

• 대한설비공학회지:설비저널
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• 제17권3호
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• pp.249-256
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• 1988

The purpose of this paper is to research the estimations of the indoor natural temperature in a case of the earth sheltered space and the 1st basement room in comparison with a conventional housing. The result of this study can be summerized as follows: The natural temperature of the earth sheltered house Summer : $${\theta}es=27.0+1.65sin(2{\pi}/24{\cdot}T-1.34)$$ Winter : $${\theta}ew=11.5+1.15sin(2{\pi}/24{\cdot}T-1.61)$$ The natural temperature of the 1st basement space Summer : $${\theta}us=25.5+1.00sin(2{\pi}/24{\cdot}T-1.72)$$ Winter : $${\theta}uw=13.9+1.10sin(2{\pi}/24{\cdot}T-2.29)$$ From the results of the stated above, we can calculate the cooling and heating load in the earth sheltered house and the underground space exactly and easily at Taejeon City.

• Applied Biological Chemistry
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• 제48권4호
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• pp.339-344
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• 2005

도열병은 곰팡이 균(Magnaporthe grisea)에 의해 발병되는 것으로, 벼 수확량에 가장 큰 손실을 일으킨다. 본 연구에서는 벼 도열병 저항성 신호전달 체계에 관여하는 유전자를 분리하기 위하여 DEB(1, 3-Butadiene diepoxide) 처리를 통하여 벼 도열병 저항성 품종인 RIl260의 돌연변이 2,000여 종을 생산하고, 이들로부터 병 저항성 변이 개체를 조사하였다. 돌연변이 집단에서 백변종 돌연변이의 비율은 6.7%로 매우 높았으며, 이것은 DEB 처리에 의해서 생산된 집단 내에 돌연변이가 높은 빈도로 발생하였음을 보여준다. 돌연변이 집단의 병 저항성 분석을 통하여 완전히 혹은 부분적으로 벼 도열병에 저항성을 상실한 29개의 돌연변이체를 분리하였다. 이들 중에서 가장 심한 이병성 라인으로 확인된 M5465는 DNA 혼성화 반응 분석을 사용하여 분석하였으며, RIL260 품종에서 도열병 병 저항성과 밀접한 관련을 갖고 있는 것으로 보고된 Pi5(t) 유전좌위의 DNA 표지들이 실험에 사용되었다. 이 결과들은 M5465에서 Pi5(t) 유전좌위 내부에 DNA의 큰 결손 및 재배열이 있었음을 보여준다. 분리된 병 저항성 돌연변이 라인들은 Pi5(t)에 의해 매개된 도열병 저항성의 신호 전달 과정을 이해하는 데 유용하게 사용 될 수 있을 것이다.

• Journal of Astronomy and Space Sciences
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• 제30권1호
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• pp.25-32
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• 2013

We statistically investigated the properties of low-latitude Pi2 pulsations using Bohyun (BOH, Mlat = $29.8^{\circ}$, L = 1.35) ground magnetometer data in 2008. For this 1-year interval, 582 Pi2 events were identified when BOH was in the nightside from 1800 to 0600 local times. We found the following Pi2 characteristics. (1) The occurrence distribution of Pi2s is relatively constant in local times. (2) The Pi2 frequency varies in local times. That is, Pi2 pulsations in postmidnight sector had higher frequency than in premidnight sector. (3) Pi2 power in premidnight sector is stronger than in postmidnight sector. (4) Pi2 frequency has positive correlation with solar wind speed and AE index. (5) Pi2 power has not a clear correlation with solar wind parameters. This indicates that Pi2 power is not controlled by external sources. (6) It is found that the most probable-time between Pi2 onsets is ${\Delta}t$ ~ 37.5 min: This is interpreted to be the period between Pi2 pulsations when they occur cyclically. We suggest that ${\Delta}t$ ~ 37.5 min is the occurrence rate of reconnection of open field lines in the tail lobe.

• 한국농공학회지
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• 제6권1호
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• pp.737-749
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• 1964

This is an attempt to find out the periodicity of the natural hydrological phenomena by the function of vibration periodicity, under the assumption that the phenomena are periodic. The result of this study at Suwon is as foIlows: 1. Annual precipitation and tota1 precipitation during summer season have the periodicity of five years. 2. Annual temperature and tota1 temperature during winter season have the periodicity of seven years. 3. The regulation curve equations of the above vibration phenomena are as foIlows: a Annual precipitation. Y = 1149-250cos2/5${\PI}$t-33 sin 2/5 t b. Total precipitation during summer season. Y=212'.9+33.06sin (2/5${\PI}$t+$88^{\circ}$13') c. Annual temperature. Y= 140.3+3.3 sin (2/7${\PI}$t+ $154^{\circ}C$55')

• 한국육종학회지
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• 제40권3호
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• pp.215-222
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• 2008

Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.

• 한국수산과학회지
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• 제22권5호
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• pp.281-290
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• 1989

1987년 $1\~2$월에 걸쳐 동지나해에서 대형기저쌍끌이어업에 의해 어획된 덕대를 대상으로 연령, 성장에 관하여 조사하였다. 연령사정은 추체로 하였으며, 추체경에 대한 연역지수에 의한 윤문의 형성은 $9\~10$월에 걸쳐 연1회 형성되었다. 추체경(R)에 대한 체장(FL)과의 관계는 암컷 : $FL=8.8262R^{0.7709}$ 수컷 ; $FL=8.9676R^{0.7976}$ 전체 ; $FL=8.7883R^{0.7681}$과 같다. 체장(FL)에 대한 체중(BW)과의 관계는 암컷 : $BW=0.0093FL^{3.3795}$ 수컷 ; $BW=0.0081FL^{3.4153}$ 전체 : $BW=0.0084FL^{3.4125}$와 같다. 연령(t)에 대한 체장($L_t$)의 버틀란피 성장식은 암컷 : $$L_t=29.17(1-e xp(-0.3213(t+0.7601)))$$ 수컷 ; $$L_t= 28.21(1-e xp(-0.3L69(t+0.8284)))$$ 전체 : $$L_t=29.00(1-e xp(-0.3228(t+0.7361)))$$와 같다. 연령(t)에 대한 체중($W_t$)의 버틀란피 성장식은 암컷; $$W_t=829.4(1-e xp(-0.3213(t+0.7601)))^{3.3795}$$ 수컷; $$W_t=747.6(1-e xp(-0.3169(t+0.8284)))^{3.4154}$$ 전체 : $$W_t=816.8(1-e xp(-0.3228(t+ 0.7361)))^{3.4125}$$와 같다. 월별 연령별 평균체장($L_t$)에 대한 주기변동 버틀란피 성장식은 암컷 : $$L_t=29.2(1-e xp(-0.3458(t+0.8182)+(0.3458/2\pi) sin2\pi(t+0.027)))$$ 수컷 : $$L_t=28.2(1-e xp(-0.2789(t+1.5193)+(0.2789/2\pi) sin2\pi(t-0.1062)))$$와 같다.

• Parasites, Hosts and Diseases
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• 제51권5호
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• pp.583-588
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• 2013

To determine alteration of immune responses during visceral larva migrans (VLM) caused by Toxascaris leonina at several time points, we experimentally infected mice with embryonated eggs of T. leonina and measured T-helper (Th) cell-related serial cytokine production after infection. At day 5 post infection (PI), most larvae were detected from the lungs, spleen, intestine, and muscle. Expression of thymic stromal lymphopoietin (TSLP) and CCL11 (eotaxin) showed a significant increase in most infected organs, except the intestine. However, expression of the CXCL1 (Gro-${\alpha}$) gene was most highly enhanced in the intestine at day 14 PI. Th1-related cytokine secretion of splenocytes showed increases at day 28 PI, and the level showed a decrease at day 42 PI. Th2-related cytokine secretion of splenocytes also showed an increase after infection; in particular, IL-5 level showed a significant increase at day 14 PI, and the level showed a decrease at day 28 PI. However, levels of Th17-related cytokines, IL-6 and IL-17A, showed gradual increases until day 42 PI. In conclusion, Th1, Th2, and Th17-related cytokine production might be important in immune responses against T. leonina VLM in experimental mice.