Analysis of Korean japonica rice cultivars using molecular markers associated with blast resistance genes

  • Received : 2008.06.18
  • Published : 2008.09.10

Abstract

Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.

Keywords

Acknowledgement

Supported by : RDA

References

  1. Causse MA, Fulton TM, Cho YG, Ahn SN, Chunwonges J, Wu K, Sian Z, Yu Z, Ronald PC, Harrington SE, Second G, McCouch SR, Tanksly SD. 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics. 138:1251-1274
  2. Chen DH, dela Vina M, Inukai T, Mackill DJ, Ronald PC, Nelson RJ. 1999. Molecular mapping of the rice blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar. Theor Appl Genet. 98:1046-1053 https://doi.org/10.1007/s001220051166
  3. Chen X, Temnykh S, Xu Y, Cho YG, McCouch SR. 1997. Development of a microsatellite framework map providing genome-wide coverge in rice (Oryza sativa L.). Theor Appl Genet. 95:553-567 https://doi.org/10.1007/s001220050596
  4. Cho YC, Baek MK, Suh JP, Kwon SJ, Choi IS, Kim YG, Han SS, Choi HC, Hwang HG. 2004. Mapping of quantitative resistance genes to leaf blast in a japonica rice cultivar, Suweon365. Korean J. Breed. 36(3):125-132
  5. Cho YC, Kwon SW, Choi IS, Lee SK, Jeon JS, Oh MK, Roh JH, Hwang HG, Yang SJ, Kim YG. 2007. Identification of major blast resistance genes in Korean rice varieties (Oryza sativa L.) using molecular markers. J. Crop Sci. Biotech. 10(4):265-276
  6. Cho YC, Roh JH, Kim BR, Choi IS, Kim MK, Han SS, Fukuta Y, Hwang HG, Kim YG. 2005. Reaction of resistance genes of monogenic lines to rice blast (Magnaporthe grisea). Korean J. Breed. 37(3):155-161
  7. Choi JE, Park JS, Park NK. 1989. Resistance of varieties to rice blast in Korea, I. Japonica type of rice varieties. J. Agri. Sci. Chungnam Natl. Univ. 16:1-18
  8. Conaway-Bormans CA, Marchetti MA, Johnson CW, McClung AM, Park WD. 2003. Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selection. Theor Appl Genet. 107:1014-1020 https://doi.org/10.1007/s00122-003-1338-5
  9. Han SS, Ryu JD, Shim HS, Lee SW, Hong YK, Cha KH. 2001. Breakdown of resistance of rice cultivars by new race KI1117a and race distribution of rice blast fungus during 1999-2000 in Korea. Korean Research in Plant Disease. 7(2):86-92
  10. Hayashi K, Hashimoto N, Daigen M, Ashikawa I. 2004. Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus. Theor Appl Genet. 108:1212-1220 https://doi.org/10.1007/s00122-003-1553-0
  11. Jeon JS, Chen D, Yi GH, Wang GL, Ronald PC. 2003. Genetic and physical mapping of Pi5(t), a locus associated with broadspectrum resistance to rice blast. Mol Gen Genomics. 269:280-289
  12. Jia Y, Wang Z, Singh P. 2002. Development of dominant rice blast Pi-ta resistacne gene markers. Crop Sci. 42:2145-2149 https://doi.org/10.2135/cropsci2002.2145
  13. Kiyosawa S. 1967. The inheritance of resistance of the Zenith type varieties of rice to the blast fungus. Japanese J. Breed. 17:99-107 https://doi.org/10.1270/jsbbs1951.17.99
  14. Kwon SJ, Ahn SN, Hong HC, Cho YC, Suh JP, Kim YG, Kang KH, Han SS, Choi HC, Moon HP, Hwang HG. 2002. Identification of DNA markers linked to resistance genes to rice blast (Pyricularia grisea Sacc.). Korean J. Breed. 34(2):105-110
  15. Kwon SW, Cho YC, Kim YG, Suh JP, Jeung JU, Roh JH, Lee SK, Jeon JS, Yang SJ, Lee YT. 2008. Development of nearisogenic japonica rice lines with enhanced resistance to Magnaporthe grisea. Mol Cells. 25(3):407-416
  16. Liu G, Lu G, Zeng L, Wang GL. 2002. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Mol Genet Genomics. 267:472-480 https://doi.org/10.1007/s00438-002-0677-2
  17. Panaud O, Chen X, McCouch SR. 1996. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet. 252:597-607
  18. Rural Development of Administration (RDA) 1994-2003. Annual report of joint study on development of new varieties. Book (Korean version)
  19. Rohlf FJ. 1993. NTSYS-PC numerical taxonomy and multivariate analysis system. Version 2.0j. Exter. Publ. Setauket, New York
  20. Sallaud C, Lorieux M, Roumen E, Tharreau D, Berruyer R, Svestasrani P, Garsmeur O, Ghesquiere A, Notteghem J-L. 2003. Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy. Theor Appl Genet. 106:794-803 https://doi.org/10.1007/s00122-002-1088-9
  21. Suh JP, Choi YH, Kim KJ, Cho YC, Kwon SJ, Jeong YP, Jeung JU, Choi IS, Kim YG, Choi HC, Hwang HG. 2004. Genetic diversity and QTLs for grain quality in japonica rice. Korean J. Breed. 36(1):31-37
  22. Tsunematsu H, Yanoria MJT, Ebron LA, Hayashi N, Ando I, Kato H, Imbe T, Khush GS. 2000. Development of monogenic lines of rice blast resistance. Breeding Sci. 50:229-234 https://doi.org/10.1270/jsbbs.50.229
  23. Wang GL, Mackill DJ, Bonman JM, McCouch SR, Champoux MC, Nelson RJ. 1994. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics. 136:1421-1434
  24. Wang GL, Ruan DL, Song WY, Sideris S, Chen L, Pi LY, Zhang S, Zhang Z, Fauquet C, Gaut BS, Whalen MC, Ronald PC. 1998. Xa21D encodes a receptor-like molecule with a leucine-rich repeat domain that determines race-specific recognition and is subject to adaptive evolution. Plant Cell. 10:765-779 https://doi.org/10.1105/tpc.10.5.765
  25. Wang ZX, Yano M, Yamanouchi U, Iwamoto M, Monna L, Hayasaka H, Katayose Y, Sasaki T. 1999. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes. Plant J. 19:55-64 https://doi.org/10.1046/j.1365-313X.1999.00498.x
  26. Wu JL, Sinha OK, Variar M, Zheng KL, Leach JE, Courtois B, Leung H. 2004. Association between molecular markers and blast resistance in an advanced backcross population of rice. Theor Appl Genet. 108:1024-1032 https://doi.org/10.1007/s00122-003-1528-1
  27. Yaegashi H. 1994. Use of resistant varieties and disease control for paddy Agric Hortic. 69(1):149-154
  28. Yi G, Lee SK, Hong YK, Cho YC, Nam MH, Kim SC, Han SS, Wang GL, Hahn TR, Ronald PC, Jeon JS. 2004. Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea. Theor Appl Genet. 109: 978-985 https://doi.org/10.1007/s00122-004-1707-8
  29. Yokoo M. 2005. Introduction of Piz-t and Pib genes for blast resistance from indica varieties and their utilization for genetic researches in rice. Japanese Agri. Res. Quarterly. 39:239-245 https://doi.org/10.6090/jarq.39.239