KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Screening of the Dominant Rice Blast Resistance Genes with PCR-based SNP and CAPS Marker in Aromatic Rice Germplasm

  • Kim, Jeong-Soon (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Sang-Nag (Depatment of Agronomy, Chungnam National University) ;
  • Hong, Sung-Jun (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kwon, Jin-Hyeuk (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Kim, Yeong-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jee, Hyeong-Jin (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Shim, Chang-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
  • Received : 2011.09.21
  • Accepted : 2011.11.11
  • Published : 2011.12.30
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Abstract

The objective of this study was to determine the genetic diversities of major rice blast resistance genes among 84 accessions of aromatic rice germplasm. Eighty four accessions were characterized by a dominant 11 set of PCR-based SNP and CAPS marker, which showed the broad spectrum resistance and closest linkage to seven major rice blast resistance (R) genes, Pia, Pib, Pii, Pi5 (Pi3), Pita (Pita-2), and Pi9 (t). The allele specific PCR markers assay genotype of SCAR and STS markers was applied to estimate the presence or absence of PCR amplicons detected with a pair of PCR markers. One indica accession, Basmati (IT211194), showed the positive amplicons of five major rice blast resistance genes, Pia, Pi5 (Pi3), Pib, Pi-ta (Pi-ta2), and Pik-5 (Pish). Among 48 accessions of the PCR amplicons detected with yca72 marker, only five accessions were identified to Pia gene on chromosome 11. The Pib gene was estimated with the NSb marker and was detected in 65 of 84 accessions. This study showed that nine of 84 accessions contained the Pii gene and owned Pi5 (Pi3) in 42 of 84 accessions by JJ817 and JJ113-T markers, which is coclosest with Pii on chromosome 9. Only six accessions were detected two alleles of the Pita or Pita-2 genes. Three of accessions were identified as the Pi9 (t) gene locus.

Keywords

References

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