• Journal of Life Science
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• v.32 no.2
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• pp.86-93
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• 2022

Lupeol is a type of pentacyclic triterpene and has been reported to have pharmacological activities against various diseases; however, the effect of lupeol on glucose absorption has not been elucidated yet. This study aimed to investigate the effect of lupeol on glucose uptake in 3T3-L1 adipocytes. Lupeol significantly facilitated glucose uptake by translocating glucose transporter type 4 (GLUT4) to the plasma membrane of the 3T3-L1 adipocytes, which was related to activation of the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) and 5 'adenosine monophosphate-activated protein kinase (AMPK) pathways. In the PI3K/AKT pathway, lupeol stimulates the phosphorylation of insulin receptor substrate 1 (IRS-1), which activates PI3K. Its activation by lupeol promotes the phosphorylation of AKT, but not the atypical protein kinase C isoforms ζ and λ. Lupeol also promoted the phosphorylation of AMPK. The activation of AMPK increased the expressions of the plasma membrane GLUT4 and the intracellular glucose uptake. The increase in the glucose uptake by lupeol was suppressed by wortmannin (PI3K inhibitor) and compound C (AMPK inhibitor) in the 3T3-L1 adipocytes. The results indicate that lupeol can facilitate glucose uptake by increasing insulin sensitivity through the stimulation of the expression of plasma membrane glucose transporter type 4 via the PI3K/AKT and AMPK pathways in the 3T3-L1 adipocytes.

• International Journal of Advanced Culture Technology
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• v.5 no.1
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• pp.70-75
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• 2017

Recent development of IT industry including smart phones and communication technologies has resulted in rapid growth of Internet of Things (IoT) technology and relevant markets. The access to IoT is becoming easier thanks to the boards and IoT products, such as Arduino and Raspberry Pi. Large-scale business sites use IoT technology to manage inventories, but small-scale business sites do not. In the present study, We ported Linux-based Raspbian to Raspberry Pi, It utilizes web server communication to control the Arduino through the application We used a color sensor to figure out the kind of inventory. We also built a database using MySQL to store the data. We used raspberry pi to check whether the proposed system works and apply it to small-scale business.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.4
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• pp.329-341
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• 2011

The objective of this study was to determine the genetic diversities of major rice blast resistance genes among 84 accessions of aromatic rice germplasm. Eighty four accessions were characterized by a dominant 11 set of PCR-based SNP and CAPS marker, which showed the broad spectrum resistance and closest linkage to seven major rice blast resistance (R) genes, Pia, Pib, Pii, Pi5 (Pi3), Pita (Pita-2), and Pi9 (t). The allele specific PCR markers assay genotype of SCAR and STS markers was applied to estimate the presence or absence of PCR amplicons detected with a pair of PCR markers. One indica accession, Basmati (IT211194), showed the positive amplicons of five major rice blast resistance genes, Pia, Pi5 (Pi3), Pib, Pi-ta (Pi-ta2), and Pik-5 (Pish). Among 48 accessions of the PCR amplicons detected with yca72 marker, only five accessions were identified to Pia gene on chromosome 11. The Pib gene was estimated with the NSb marker and was detected in 65 of 84 accessions. This study showed that nine of 84 accessions contained the Pii gene and owned Pi5 (Pi3) in 42 of 84 accessions by JJ817 and JJ113-T markers, which is coclosest with Pii on chromosome 9. Only six accessions were detected two alleles of the Pita or Pita-2 genes. Three of accessions were identified as the Pi9 (t) gene locus.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.17 no.3
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• pp.249-256
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• 1988

The purpose of this paper is to research the estimations of the indoor natural temperature in a case of the earth sheltered space and the 1st basement room in comparison with a conventional housing. The result of this study can be summerized as follows: The natural temperature of the earth sheltered house Summer : $${\theta}es=27.0+1.65sin(2{\pi}/24{\cdot}T-1.34)$$ Winter : $${\theta}ew=11.5+1.15sin(2{\pi}/24{\cdot}T-1.61)$$ The natural temperature of the 1st basement space Summer : $${\theta}us=25.5+1.00sin(2{\pi}/24{\cdot}T-1.72)$$ Winter : $${\theta}uw=13.9+1.10sin(2{\pi}/24{\cdot}T-2.29)$$ From the results of the stated above, we can calculate the cooling and heating load in the earth sheltered house and the underground space exactly and easily at Taejeon City.

• Applied Biological Chemistry
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• v.48 no.4
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• pp.339-344
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• 2005

Rice blast, which is caused by the fungus Magnaporthe grisea, is one of the most destructive diseases of rice. To identify genes involving in the signal transduction pathways that mediate rice blast resistance, we screened over 2,000 mutant lines of a highly resistant variety RIL260 that were generated by using a DEB (1, 3-Butadiene diepoxide) treatment method. In the mutant population, the frequency of albino plants was 6.7%, indicating that this population has a high frequency of mutations in the genome. The primary screening identified 29 mutant plants that exhibit a complete or partial loss of the resistance to rice blast. Among them, M5465, the most susceptible line, was subsequently examined by DNA gel-blot experiments using DNA molecular markers of Pi5(t) that has been previously identified as a durable resistance locus in RIL260. The result revealed that a large deletion and rearrangement of genomic DNA occurred in the Pi5(t) locus. The results suggest that DEB can be used as an efficient mutagen to induce large scale mutations in the rice genome. The isolated mutants should be useful for elucidating the Pi5(t)-mediated signaling pathways of rice blast resistance.

• Journal of Astronomy and Space Sciences
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• v.30 no.1
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• pp.25-32
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• 2013

We statistically investigated the properties of low-latitude Pi2 pulsations using Bohyun (BOH, Mlat = $29.8^{\circ}$, L = 1.35) ground magnetometer data in 2008. For this 1-year interval, 582 Pi2 events were identified when BOH was in the nightside from 1800 to 0600 local times. We found the following Pi2 characteristics. (1) The occurrence distribution of Pi2s is relatively constant in local times. (2) The Pi2 frequency varies in local times. That is, Pi2 pulsations in postmidnight sector had higher frequency than in premidnight sector. (3) Pi2 power in premidnight sector is stronger than in postmidnight sector. (4) Pi2 frequency has positive correlation with solar wind speed and AE index. (5) Pi2 power has not a clear correlation with solar wind parameters. This indicates that Pi2 power is not controlled by external sources. (6) It is found that the most probable-time between Pi2 onsets is ${\Delta}t$ ~ 37.5 min: This is interpreted to be the period between Pi2 pulsations when they occur cyclically. We suggest that ${\Delta}t$ ~ 37.5 min is the occurrence rate of reconnection of open field lines in the tail lobe.

• Magazine of the Korean Society of Agricultural Engineers
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• v.6 no.1
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• pp.737-749
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• 1964

This is an attempt to find out the periodicity of the natural hydrological phenomena by the function of vibration periodicity, under the assumption that the phenomena are periodic. The result of this study at Suwon is as foIlows: 1. Annual precipitation and tota1 precipitation during summer season have the periodicity of five years. 2. Annual temperature and tota1 temperature during winter season have the periodicity of seven years. 3. The regulation curve equations of the above vibration phenomena are as foIlows: a Annual precipitation. Y = 1149-250cos2/5${\PI}$t-33 sin 2/5 t b. Total precipitation during summer season. Y=212'.9+33.06sin (2/5${\PI}$t+$88^{\circ}$13') c. Annual temperature. Y= 140.3+3.3 sin (2/7${\PI}$t+ $154^{\circ}C$55')

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.215-222
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• 2008

Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.22 no.5
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• pp.281-290
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• 1989

Growth of the Korean pomfret, Pampus echinogaster, captured in the East China Sea from January to December 1987, was estimated by annual rings on the vertebral centrum. The time of annual ring formation was approximately during September and October. Von Ber-talanffy growth equations derived from the mean back-calculated fork length(cm) at each age were as follows: for females, $L_{t}=29.17(1-e xp(-0.3213(t+0.7601)))$, for males, $L_t=28.21(1-e xp(-0.3169(t+0.8284)))$, and for sexes combined, $L_t=29.00(1-e xp(-0.3228(t+0.7361)))$, Seasonal growth equations derived from the mean observed fork length (cm) at age by month were as follows: for females, $L_t=29.2(1-e xp(-0.3458(t+0.8182)+(0.3458/2\pi)sin2\pi(t+0.027)))$, and for males, $L_t=28.2(1-e xp(-0.2789(t+1.5913)+(0.2789/2\pi)sin2\pi(t-0.1062)))$.

• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.583-588
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• 2013

To determine alteration of immune responses during visceral larva migrans (VLM) caused by Toxascaris leonina at several time points, we experimentally infected mice with embryonated eggs of T. leonina and measured T-helper (Th) cell-related serial cytokine production after infection. At day 5 post infection (PI), most larvae were detected from the lungs, spleen, intestine, and muscle. Expression of thymic stromal lymphopoietin (TSLP) and CCL11 (eotaxin) showed a significant increase in most infected organs, except the intestine. However, expression of the CXCL1 (Gro-${\alpha}$) gene was most highly enhanced in the intestine at day 14 PI. Th1-related cytokine secretion of splenocytes showed increases at day 28 PI, and the level showed a decrease at day 42 PI. Th2-related cytokine secretion of splenocytes also showed an increase after infection; in particular, IL-5 level showed a significant increase at day 14 PI, and the level showed a decrease at day 28 PI. However, levels of Th17-related cytokines, IL-6 and IL-17A, showed gradual increases until day 42 PI. In conclusion, Th1, Th2, and Th17-related cytokine production might be important in immune responses against T. leonina VLM in experimental mice.