• The Korean Journal of Physiology and Pharmacology
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• v.21 no.6
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• pp.579-589
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• 2017

Anesthetics are used extensively in surgeries and related procedures to prevent pain. However, there is some concern regarding neuronal degeneration and cognitive deficits arising from regular anesthetic exposure. Recent studies have indicated that brain-derived neurotrophic factor (BDNF) and cyclic AMP response element-binding protein (CREB) are involved in learning and memory processes. Genistein, a plant-derived isoflavone, has been shown to exhibit neuroprotective effects. The present study was performed to examine the protective effect of genistein against isoflurane-induced neurotoxicity in rats. Neonatal rats were exposed to isoflurane (0.75%, 6 hours) on postnatal day 7 (P7). Separate groups of rat pups were orally administered genistein at doses of 20, 40, or 80 mg/kg body weight from P3 to P15 and then exposed to isoflurane anesthesia on P7. Neuronal apoptosis was detected by TUNEL assay and FluoroJade B staining following isoflurane exposure. Genistein significantly reduced apoptosis in the hippocampus, reduced the expression of proapoptotic factors (Bad, Bax, and cleaved caspase-3), and increased the expression of Bcl-2 and Bcl-xL. RT-PCR analysis revealed enhanced BDNF and TrkB mRNA levels. Genistein effectively upregulated cAMP levels and phosphorylation of CREB and TrkB, leading to activation of cAMP/CREB-BDNF-TrkB signaling. PI3K/Akt signaling was also significantly activated. Genistein administration improved general behavior and enhanced learning and memory in the rats. These observations suggest that genistein exerts neuroprotective effects by suppressing isoflurane-induced neuronal apoptosis and by activating cAMP/CREB-BDNF-TrkB-PI3/Akt signaling.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.132-132
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• 2015

Virtual metrology (VM) model based on plasma information (PI) parameter for C4F8 plasma-assisted oxide etching processes is developed to predict and monitor the process results such as an etching rate with improved performance. To apply fault detection and classification (FDC) or advanced process control (APC) models on to the real mass production lines efficiently, high performance VM model is certainly required and principal component regression (PCR) is preferred technique for VM modeling despite this method requires many number of data set to obtain statistically guaranteed accuracy. In this study, as an effective method to include the 'good information' representing parameter into the VM model, PI parameters are introduced and applied for the etch rate prediction. By the adoption of PI parameters of b-, q-factors and surface passivation parameters as PCs into the PCR based VM model, information about the reactions in the plasma volume, surface, and sheath regions can be efficiently included into the VM model; thus, the performance of VM is secured even for insufficient data set provided cases. For mass production data of 350 wafers, developed PI based VM (PI-VM) model was satisfied required prediction accuracy of industry in C4F8 plasma-assisted oxide etching process.

• The Korean Journal of Food And Nutrition
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• v.9 no.4
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• pp.452-458
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• 1996

A PPIase gene of Bacillus stearothermophilus was screened from a genomic library by plaque hybridization using the A-1 primer as a probe. A PPIase positive plaque contained a 3.0kb insert of the chromosomal DNA. A 3.0kb fragment was subcloned into pUC18, resulting pPI1-40. A DNA fragment encoding the N-terminal portion of the PPIase in pPi-40 was amplified by polymerase chain reaction(PCR) method using the A-1 and B-2 primers. The amplified fragment was cloned into the Sma I site of pUC18 and recombinant plasmid was designated as pSN-18. The nucleotide sequence of 167bp fragment was determined. The deduced amino acid sequence of PPIase was completely matched with the determined N-terminal amino acid sequence of PPIase B. stearothermophilus. The translated protein sequence of PPIase B. stearothermophilus was compared with sequence from periplasmic PPIase from Escherichina coil ; homogies of 16 and 58%, respectively, were found. The clond PPIase gene was over-expressed in E. coil cell using pUC19 as an expression vector. The enzyme was partially purified by heat treatment and colum chromatochraphy on DEAE-Sepharose CL-6B. The molecular weight of the enzyme was dermined to be about 18.0 kDal by SDS-PAGE.

• Korean Journal of Mathematics
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• v.15 no.2
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• pp.149-165
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• 2007

We investigate the existence of multiple nontrivial solutions $u(x,t)$ for a perturbation $b[({\xi}-{\eta}+2)^+-2]$ of the hyperbolic system with Dirichlet boundary condition $$(0.1)\;L{\xi}={\mu}[({\xi}-{\eta}+2)^+-2]\;in\;({-{\frac{{\pi}}{2}}},{\frac{{\pi}}{2}}){\times}\mathbb{R},\\L{\eta}={\nu}[({\xi}-{\eta}+2)^+-2]\;in\;({-{\frac{{\pi}}{2}}},{\frac{{\pi}}{2}}){\times}\mathbb{R},$$, where $u^+$=max{u,o}, ${\mu}$, ${\nu}$ are nonzero constants. Here L is the wave operator in $\mathbb{R}^2$ and the nonlinearity $({\mu}-{\nu})[({\xi}-{\eta}+2)^+-2]$ crosses the eigenvalues of the wave operator.

• Honam Mathematical Journal
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• v.29 no.4
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• pp.723-732
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• 2007

We investigate the existence of solutions u(x, t) for a perturbation b[$(\xi+\eta+1)^+-1$] of the hyperbolic system with Dirichlet boundary condition (0.1) = $L\xi-{\mu}[(\xi+\eta+1)^+-1]+f$ in $(-\frac{\pi}{2},\frac{\pi}{2}\;{\times})\;\mathbb{R}$, $L\eta={\nu}[(\xi+\eta+1)^+-1]+f$ in $(-\frac{\pi}{2},\frac{\pi}{2}\;{\times})\;\mathbb{R}$ where $u^+$ = max{u,0}, ${\mu},\nu$ are nonzero constants. Here $\xi,\eta$ are periodic functions.

• The Pure and Applied Mathematics
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• v.17 no.3
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• pp.257-268
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• 2010

We investigate the existence of the following Dirichlet boundary value problem $({\mid}u'\mid^{p-2}u')'\;+\;(p\;-\;1)[\alpha{\mid}u^+\mid^{p-2}u^+\;-\;\beta{\mid}u^-\mid^{p-2}u^-]$ = (p - 1)h(t), u(0) = u(T) = 0, where p > 1, $\alpha$ > 0, $\beta$ > 0 and ${\alpha}^{-\frac{1}{p}}\;+\;{\beta}^{-\frac{1}{p}}\;=\;2$, $T\;=\;{\pi}_p/{\alpha}^{\frac{1}{p}}$, ${\pi}_p\;=\; \frac{2{\pi}}{p\;sin(\pi/p)}$ and $h\;{\in}\;L^{\infty}$(0,T). The results of this paper generalize some early results obtained in [8] and [9]. Moreover, the method used in this paper is elementary and new.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.2
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• pp.376-383
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• 2016

In this paper, the blue-green algae monitoring program of applying IoT(Internet of things) technologies is designed and implemented that can check out the status of the river's water quality in real time. The proposed system is to extract the image data from the camera of raspberry pi by an wireless network, and it is analyzed through the HSV color model. We measure the temperature using a DS18B20 1-wire temperature sensor. The extracted information of image data and temperature is then analyzed in C and Python programs for use with Raspberry Pi. The XML data in PHP program is made from the analyzed information and provides Web services. It also allows to refer the XML data using mobile devices.

• International Journal of Automotive Technology
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• v.7 no.1
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• pp.91-99
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• 2006

The diesel Auxiliary Power Unit (APU) for vehicle applications is a complex nonlinear system. For the purpose of this paper presents a dynamic average model of the whole system in an entirely physical way, which accounts for the non-ideal behavior of the diode rectifier, the nonlinear phenomena of generator-rectifier set in an elegant way, and also the dynamics of the dc load and diesel engine. Simulation results show the accuracy of the model. Based on the average model, a simple PI control scheme is proposed for the multivariable system, which includes the steps of model linearization, separate PI controller design with robust tuning rules, stability verification of the overall system by considering it as an uncertain one. Finally it is tested on a detailed switching model and good performances are shown for both set-point following and disturbance rejection.

• Proceedings of the Korea Information Processing Society Conference
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• 2019.10a
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• pp.228-230
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• 2019

일상생활에서 빈번히 사용되는 주변 생활 제품은 기술의 급속한 발전과 더불어 지능화 정도가 가속화 되고 있는 추세에 있다. 일반적으로 LED 조명이나 실내 에어컨을 자동으로 조정하거나 자동청소로봇 등은 이미 일반화된 제품들이다. 그럼에도 불구하고 우리 생활에서 가장 필요한 용품인 거울에 대한 지능적인 제품은 비교적 고가이어서 소비자가 쉽게 접근하기 어려운 생활용품이 되고 있어서 지능화 제품의 보급이 더딘편이다. 따라서 본 논문에서는 Raspberry Pi 3B+ 를 기반으로 하여 음성제어가 가능한 스마트 미러를 설계하고 구현하였다. 이를 위하여 저렴한 raspberry pi의 WiFi를 통해 네트워크에 연결하도록 하여 미러가 시간, 날씨 및 뉴스 정보 기능을 자동으로 업데이트 할 수 있도록 하였고 기상 조건, 사전 시간 또는 음악 재생과 같은 음성 제어가 가능하기 위하도록 Google Asistant 음성 인터페이스를 적용하였다. 본 논문에서 제안한 제품이 실용화될 경우 저가이면서 고기능 사양을 제공하고 있어서 스마트 미러 보급에 많은 기여가 예산된다.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.113-122
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• 2014

A5E is complex of several medicinal herb ethanol extracts. The aim of this study is investigating the anticancer effect for non-small cell lung cancer. The antitumor effects of A5E on NCI-H460 were examined by regulation of cell proliferation, apoptosis, cell cycle arrest, mitochondrial membrane potential (${\Delta}{\Psi}_m$), and apoptosis-related protein. Cell proliferation was measured by MTS assay. Apoptosis induced by A5E was confirmed by Annexin V-fluorescein isothiocyanate (FITC)/Propidium Iodide (PI) staining, and cell cycle arrest was measured by PI staining. NF-${\kappa}B$ translocation was detected by immunofluorescence and MMP (${\Delta}{\Psi}_m$) was measured by JC-1 staining. The expression of extrinsic pathway molecules such as FasL and FADD were elevated, and procaspase-8 was processed by A5E. In addition, intrinsic pathway related molecules were altered. The Bcl-2 and Bcl-xl levels decreased, Bax increased, and cytochrome C was released. In addition, the mitochondrial membrane potential collapsed, and caspase-3 and poly-(ADP-ribose) polymerase were processed by A5E. Moreover, A5E affected the cellular survival pathway involving phosphatidylinositol 3-kinase (PI3K)/Akt and NF-${\kappa}B$. PI3K and Akt were downregulated, also NF-${\kappa}B$ expression was decreased, and nuclear translocalization was inhibited by A5E. These results suggested that A5E delays proliferation, inhibit cell cycle progression and induce apoptosis in human lung cancer cell. We conclude that A5E is a potential anticancer agent for human lung carcinoma.