• Applied Biological Chemistry
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• v.39 no.1
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• pp.32-38
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• 1996

Effects of microwave heating on the content of phytic acid and phosphorus of soaked soybean were investigated. Phytic acid content of Danwon, Marly and Amsoy cultivars were found to be 19.19 mg, 18.38 mg, and 16.73 mg/g defatted soybean respectively. Inorganic phosphorus content of soybeans was significantly increased during microwave heating, while phytic acid and phytate phosphorus was gradually decreased. Microwave heating was more effective than autoclaving in reducing the phytate contents. It was also found that microwave heating to soybean of low moisture content was more effective than that of high moisture content for decreasing the phytic acid content. Soaking in 2.5% sodium chloride, 2% sodium bicarbonate, and mixed salt solution for 12 hrs was not effective on reducing the contents of phytic acid and phytate phosphorus, but microwave-heating after soaking in above solutions greatly decreased the contents of phytic acid and phytate phosphorus, whereas significantly increased inorganic phosphorus of soybeans.

• Fisheries and Aquatic Sciences
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• v.7 no.2
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• pp.51-57
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• 2004

Total phosphorus contents in rice bran and soybean meal were determined to be 5.81 and $2.77\%$, respectively, and $97.2\%$ of phosphorus in rice bran and $66.4\%$ in soybean meal were presented as phytate phosphorus. Optimum pH condition for hydrolysis of phytate in rice bran and soybean was determined to be in the pH range of 3.7 and 5.3. The highest activity of phytase for hydrolysis of phytate in both samples was determined to be at $55^{\circ}C$ for rice bran and $55-60^{\circ}C$ for soybean. Hydrolysis of phytate in soybean meal at pH 5.0 increased with the co-reaction or consecutive reaction with protease; however, in rice bran hydrolysis decreased with co-reaction with protease. Phytate degradation of soybean meal in the presence of pepsin at pH 2.5 showed higher than that of rice bran. Phytate degradation of rice bran in the presence of trypsin or pancreatin at pH 7.0 increased the activity around 2-times compared with the activity in the absence of trypsin or pancreatin. The results of this study suggest that hydrolysis of phytate in rice bran or soybean meal with phytase and protease may provide an alternative process for the preparation of aquacultural feed with a low level of organic phosphorus.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.404-409
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• 2009

A total of 240 day-old broiler chicks were used to study the effects of phytase on performance and phosphorus (P) excretion from birds fed diets containing low phytate barleys formulated without inorganic P. A positive control based on Harrington barley (HB) was formulated to meet requirements for total P. Three experimental diets, based on either HB (0.39% total P with 0.28% phytate P) or the low phytate varieties LP 422 (0.36% total P with 0.14% phytate P) and LP 955 (0.40% total P with 0.01% phytate P), were formulated to be below requirements for total P by removing all the inorganic P from the diet. The four diets were fed with and without 1,000 FTU/kg phytase. Apparent P digestibility was significantly higher (p<0.01) for birds fed the low phytate barleys than for birds fed HB either supplemented or un-supplemented with inorganic P. P excretion was significantly lower (p<0.01) for birds fed HB without inorganic P than with inorganic P. P excretion was further reduced by the use of the low phytate barleys LP 422 and LP 455 (p<0.01). Phytase supplementation did not affect P excretion (p = 0.39). Body weight gain and feed intake were highest for birds fed the HB diet with inorganic P and lowest for birds fed the HB diet without inorganic P (p<0.01). Among the three low P diets, body weight gain and feed intake of broilers increased as the level of phytate in the barley declined (p<0.01). Phytase modestly increased body weight gain (p = 0.08) and feed intake (p = 0.04). The overall results of this study indicate that it may be possible to reduce the amount of inorganic P used when formulating diets with low phytate barley compared with the levels needed when formulating diets with normal phytate barley. However, it is not possible to completely replace the inorganic P in diets containing low phytate barley without impairing poultry performance. Feeding diets devoid of supplementary inorganic P in combination with low phytate barley resulted in a significant reduction in P excretion by poultry.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.197-202
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• 1999

Three barrows weighing 45.0 kg, fitted with simple T-cannulas in both the duodenum and terminal ileum, were assigned to diets in a $3{\times}3$ Latin Square design experiment to determine the effect of two calcium levels (0.8% vs 0.4%) on phytase activity and nutrient balance in growing pigs. The control diet contained 0.8% calcium, with no added inorganic phosphorus (0.45% total phosphorus) and no added phytase. The two additional experimental diets contained microbial phytase (750 phytase units/kg) and supplied either 0.8% or 0.4% calcium. With added microbial phytase, ileal and total tract digestibility of rotal phosphorus were improved by 20.9 and 13.8 percentage units, respectively (p=0.01). The apparent duodenal and ileal digestibility of phytate phosphorus were increased by 51.8 and 49.7 percentage units (p=0.01). Lowering dietary calcium in the presence of microbial phytase increased the digestibility of phytate phosphorus by an additional 10.9 (p=0.001) and 5.7 percentage units for duodenal and ileal digestibility, respectively. Supplementation with microbial phytase significantly reduced fecal excretion of nitrogen and phosphorus and increased the percentage of these nutrients retained by the pig. Lowering dietary calcium further increased the percentage of dietary phosphorus retained. Overall, reducing dietary calcium appeared to increase the effectiveness of added microbial phytase in degrading phytate phosphorus. As a result, care should be taken to avoid high levels of dietary calcium when supplementing swine diets with microbial phytase.

• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.261-264
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• 2016

In soybean (Glycine max (L.) Merr.) seeds, phosphorus (P) is primarily stored in the form of phytate, which is generally indigestible by monogastric animals such as human, pig, poultry, and fish. Thus, this study was conducted to isolate soybean mutants with high available P. Inorganic P content was assessed in a total of 1,266 ethyl methanesulfonate (EMS) $M_4$ lines from the Pungsannamul cultivar. Among the tested lines, four EMS lines (PE379, PE432, PE2205, and PE2503) showed higher mean inorganic P ($1.21-1.56gkg^{-1}$) than did the Pungsannamul cultivar ($0.90gkg^{-1}$). Additionally, six EMS lines (PE718, PE828, PE1466, PE1552, PE3378, and PE3386) showed lower mean inorganic P ($0.38-0.60gkg^{-1}$). The high inorganic P mutants isolated in this study will be further investigated for phytate and total P levels. Moreover, the high and low inorganic P lines will be utilized in a future study of the biochemical pathway of phytate.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.10
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• pp.1466-1472
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• 2012

A bacterial isolate derived from soil samples near a cattle farm was found to display extracellular phytase activity. Based on 16S rRNA sequence analysis, the strain was named Bacillus sp. T4. The optimum temperature for the phytase activity toward magnesium phytate (Mg-$InsP_6$) was $40^{\circ}C$ without 5 mM $Ca^{2+}$ and $50^{\circ}C$ with 5 mM $Ca^{2+}$. T4 phytase had a characteristic bi-hump two pH optima of 6.0 to 6.5 and 7.4 for Mg-$InsP_6$. The enzyme showed higher specificity for Mg-$InsP_6$ than sodium phytate (Na-$InsP_6$). Its activity was fairly inhibited by EDTA, $Cu^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ba^{2+}$ and $Zn^{2+}$. T4 phytase may have great potential for use as an eco-friendly feed additive to enhance the nutritive quality of phytate and reduce phosphorus pollution.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.2
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• pp.135-138
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• 1995

Soybean meal was fermented by Aspergillus usami in order to reduce phytate content. Aflatoxin B1 was not detected in the fermented soybean meal. The contents of crude protein, crude fiber, ether extract and crude ash were slightly increased following fermentation with a concomitant reduction in nitrogen free extract. Though the fermentation partly degraded proteins in the soybean meal, there was small difference in amino acid composition between the soybean meal and the fermented soybean meal. The results showed that the fermentation did not affect nutritional value of protein in soybean meal. Approximately 55% of phosphorus extracted by trichloroacetic acid was inositol hexaphosphate (phytate) in the soybean meal. The content of inositol tetra to hexaphosphates was not detected in the fermented soybean meal. These results indicated that the fermentation almost completely eliminated phytate in soybean meal. Phytase activity was not detected in the unfermented soybean meal. However, the enzyme activity in the fermented soybean meal was 167.7 U/g. When the fermented soybean meal in supplemented in formula feeds, phytase in the fermented soybean meal might partly degrade the phytate in other ingredients in the digestive tract. The fermented soybean meal is possibly used as a phytate-free protein source of feed, which contains high available phosphorus.

• Preventive Nutrition and Food Science
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• v.11 no.1
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• pp.67-72
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• 2006

To determine the amount of phytate in rice grains from various cultivars, two methods were employed and compared in respect of the accuracy and conveniency. Phytate in rice samples was extracted with HCl, and then the extracts were subjected to an anion-exchange column. Finally, the phytate in eluate was quantitated using two methods: one method is based on the complex formation between ferric ion and sulfosalicylic acid in the presence of phytate, and the other is the prior acid digestion of phytate sample, followed by the colorimetric determination of liberated phosphorus. Although two methods showed similar values of phytate in rice samples, the former method is simpler and more precise than the latter. Moreover, the former is more reliable for the samples with lower phytate levels. Especially, the dilution condition of rice sample before anion exchange column separation was important for the recovery of phytate in rice samples. Based on the former method, the amount of phytate in rice of various cultivars was estimated to range from 7.3 mg/g to 12.4 mg/g rice. This method would be useful for the determination of phytate in crop samples with a lower level of phytate, one of anti-nutrients in some agricultural plants.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.2
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• pp.227-232
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• 2013

Phytate is not only an unavailable source of phosphorus (P) for broilers but it also acts as an anti-nutrient, reducing protein and mineral absorption, increasing endogenous losses and reducing broiler performance. The objective of this study was to investigate the anti-nutritional effects of phytate by including high levels of phytase in diets not severely limited in available P. A total of 768 male Arbor Acres broilers were distributed in six treatments of eight replicate pens of 16 birds each consisting of a positive control diet (PC), positive control with 500 FTU/kg phytase, negative control (NC) diet with lower available P and calcium (Ca) levels and the same NC diet with 500, 1,000 or 1,500 FTU/kg phytase. Body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR) and mortality were determined at 21 and 35 d of age while foot ash was determined in four birds per pen at 21 d of age. FI, FCR and foot ash where not affected by the lower mineral diets at 21 d of age nor by the enzyme inclusion but broilers fed lower Ca and available P diets had lower BWG. At 35 d of age no difference was observed between broilers fed the positive or NC diets but broilers fed 500, 1,000 and 1,500 FTU/kg on top of the NC diet had better FCR than broilers fed the positive control diet. When compared to birds fed a diet adequate in P, birds fed the same diet included with 500, 1,000 and 1,500 FTU/kg of phytase in marginally deficient available P and Ca diets had an improvement of performance. These results support the concept that hydrolysing phytate and reducing the anti-nutritional effects of phytate improves bird performance on marginally deficient diets that were not covering the P requirement of birds.

• Journal of the Korean Home Economics Association
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• v.27 no.2
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• pp.75-83
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• 1989

In this study, effect of heat treatment on phytic acid, phosphorus compounds, and minerals in Korean soybean varieties was investigated. Results were summarized as follwo: 1. In the soybeans tested, protein content ranged from 34.6 to 44.6%, lipid content from 15.4 to 20.2%, fiber content from 4.8 to 6.1% and ash content from 4.5 to 5.9%. 2. Content range for phytic acid in soybean varieties was 1,300 to 1,542mg/100g and its mean was measured 1,392mg/100g. With increasing of the temperature, the phytic acid tends to be destroyed, especially at 6$0^{\circ}C$ the loss was averaged about 20%. 3. Total phosphorus content in soybean ranged from 607 to 681mg/100g and the decending order of phosphors content in soybean varieties was Millyang > Hwangkeum > Kwangkyo > Danyoup > Hill > Jangyoup. It was also destroyed with increasing temperature. 4. Phytate phosphorus content range in soybean was from 315.6 to 318.0mg/100g and decreased with increasing temperature. 5. Inorganic phosphorus content ranged from 95.5 to 110.0mg/100g and it was increased by temperature rising among soybean varieties. 6. Phytate phosphorus to total phosphorus ratio ranged from 5.2 to 5.7 and decreased by temperature rising. 7. The content of nonphytate phosphorus varied widely between soybean varieties and decreased with temperature increase. 8. The content of calcium, iron and magnesium ranged from 15.7 to 25.7mg/100g from 8.8 to 16.8mg/100g and from 121.9 to 143.6mg/100g respectively. The content of Mg showed small difference among soybean varieties. The change of mineral content with heat treatment in soybean did not give any meaningful change mineral content.