• Food Science and Preservation
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• v.19 no.6
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• pp.799-806
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• 2012

This experiment was conducted to establish the optimum conditions for high $CO_2$ gas treatment in combination with a proper gas-permeable packaging film to maintain the quality of fig fruit (Ficus carica L). Among the fig fruits with different high $CO_2$ treatments, the quality change was most effectively controlled during storage in the 70%-$CO_2$-treated fig fruit. Harvested fig fruit was packaged using microperforated oriented polypropylene (MP) film to maintain the optimum gas concentrations in the headspace of packaging for the modified-atmosphere system. MP film had an oxygen transmission rate of about $10,295cm^3/m^2$/day/atm at $25^{\circ}C$. The weight loss, firmness, soluble-solid content (SSC), acidity (pH), skin color (Hunter L, a, b), and decay ratio of the fig fruits were monitored during storage at 5 and $25^{\circ}C$. The results of this study showed that the OPP film, OPP film + 70% $CO_2$, and MP film+70% $CO_2$ were highly effective in reducing the loss rate, firmness and decay occurrence rate of fig fruits that were packaged with them during storage. In the case of using treatments with packages of OPP film and OPP film+70% $CO_2$, however, adverse effects like package bursting or physiological injury of the fig may occur due to the gas pressure or long exposure to $CO_2$. Therefore, the results indicated that MP film containing 70% $CO_2$ can be used as an effective treatment to extend the freshness of fig fruits for storage at a proper low temperature.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.2
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• pp.185-192
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• 1987

A green house experiment was conducted to find out the acetylene reducing and $N_2$-fixing activity from photosynthetic and aerobic heterotrophic nitrogen fixing microorganisms in submerged paddy soil under different agricultural locality, soil series, soil texture, soil type, and drainage condition in which samples taken from without nitrogen treatment plot of NPK trials on 16 sites of the farmer's field. The results obtained were summarized as follows: 1. The highest acetylene reducing activity was observed at 7 days after incubation in the light condition (photo synthetic microbes+heterotrophic bacteria) while it was observed at 35 days incubation in the dark condition (heterotrophic bacteria). 2. Among the soil series, photosynthetic nitrogen fixing activity was pronounced more in Jangae, Ogcheon and Hwadong series while lower was obtained in Buyong and Daejeong series. Aerobic heterotrophic nitrogen fixing activity was high in Buyong and Daejong series. 3. Estimated amount of $N_2$-fixation from acetylene reducing activity was equivalented to 3.0 mg in light condition and 4.9 mg/100g/105 days in dark condition. 4. Among the agricultural locality, photosynthetic nitrogen fixing activity was high in rather warm southern part while heterotrophic nitrogen fixing activity was predominated more in mountainous area and Chungcheong continental. 5. Photosynthetic nitrogen fixing activity was predominated in high productive soil while aerobic heterotrophic nitrogen fixing activity was pronounced more in crose coarse sandy soil. 6. The soils properties of high photosynthetic nitrogen fixing activity were constituted of poorly or imperfectly drained clay or clay loam soil while heterotrophic nitrogen fixing activity was pronounced more in well to moderately well drained sandy or sandy loam soil.

• Korean Journal of Food Science and Technology
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• v.53 no.4
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• pp.423-433
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• 2021

This study was performed to examine the neuroprotective effect of the ethyl acetate fraction from Eucommia ulmoides oliver leaf (EFEL) on PM_2.5-induced cytotoxicity. EFEL had higher total phenolic and flavonoid contents than the other fractions. In ABTS and DPPH radical scavenging activities, the IC₅₀ of EFEL was measured as 212.80 and 359.13 ㎍/mL, respectively. To investigate the neuroprotective effect of EFEL, MTT and DCF-DA assays were performed on HT22, MC-IXC, and BV-2 cells. EFEL effectively decreased PM_2.5-induced intercellular reactive oxygen species (ROS) content and inhibited PM_2.5-induced cell death. In the results of protein expression related to cellular cytotoxicity on microglial cells (BV-2), EFEL had an improvement effect on cell apoptosis and inflammatory pathways. Rutin and chlorogenic acid were identified as the main physiological compounds. Moreover, it was expected that EFEL, including rutin and chlorogenic acid, could be functional food substances with neuroprotective effects against PM_2.5-induced oxidative stress.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.224-230
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• 2018

This study was conducted to investigate the effects of concentrate feeding frequency on weight change and the colic of Jeju cross-bred horses. Around thirty-six old Jeju cross-bred horses were divided into three groups and were investigated by feeding the concentrate feed at 2.5% of their body weight 2 times, 3 times, or 4 times daily. Although the total weight gain and daily weight gain for the different feeding frequencies were higher with feeding concentrates 4 times daily compared with the other treatment groups, there was no statistically significant difference between the groups (p>0.05). Rectal temperatures were above the normal range in weeks 11 to 12, but there was no statistically significant difference between the groups (p>0.05). Fecal pH was below the normal range in weeks 1 to 12, but there were no colic symptoms with any of the treatments and there was no statistically significant difference between the groups (p>0.05). When feeding concentrate feed at 2.5% of body weight, it was assumed that trying to feed it several times per day would help to prevent colic; however, there was no difference in weight gain, blood characteristics, and physiological characteristics in accordance with the number of feeding times.

• Journal of the Korean Applied Science and Technology
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• v.36 no.3
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• pp.766-778
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• 2019

In this study, Poria cocos bark were extracted by supercritical process, and anti-inflammatory, whitening, and antioxidant effects were measured in comparison with ethanol extract. Also, An effective percutaneous permeation method using a selected formulation of the extract and a drug delivery peptide was proposed. Pachymic acid, known as the anti-cancer and anti-inflammatory compound of the ventricle, is an indicator component and the HPLC analysis shows that the supercritical extract of the pericardium is more than twice that of the Poria cocos bark extract. In order to confirm antioxidative effect of Bombyx mori, DPPH scavenging ability and ABTS scavenging ability test showed that the ethanol extract of Poria cocos Back had lower concentration than the supercritical extract of Poria cocos back. However, RAW 264.7 Measurements of Nitric oxide (NO) production in cells showed lower NO production at the same concentration than the Poria cocos back ethanol extract. In addition, after 72 hours of processing of $20{\mu}g/mL$ of the Poria cocos back extract in B16 melanoma cells, both the intracellular and extracellular melanin extract were effective and the supercritical extract was lower melanin content. No toxicity was observed at the concentration of $800{\mu}g/mL$ in RAW 264.7 cells used in NO production experiments. However, in B16 melanoma cells, even at $50{\mu}g/mL$, both Poria cocos back ethanol extract and supercritical extract showed a survival rate of less than 60%. The liposome formulation and drug delivery peptides were shown to be useful for percutaneous permeation of Supercritical Extract of Poria cocos back using a liposome formulation and a drug delivery peptide. it is expected that there will be great potential for development as a variety of cosmetic materials for Poria cocos back.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.236-245
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• 2019

Fruit ripening is a genetically programmed process involving a number of biochemical and physiological processes assisted by variations in gene expression and enzyme activities. This process generally affects the phytochemical profile and the bioactive principles in fruits and vegetables. To appraise the variation in bioactive principles of fruits from Rosa rugosa during its ripening process, we analyzed the changes in antioxidant and anti-elastase activities and polyphenolic compounds during the four ripening stages of fruits. Overall, an extract of unripe fruits contained the highest levels of total phenolic and flavonoid contents, radical scavenging activity, reducing power, oxygen radical antioxidant capacity, and elastase inhibitory activity, compared with the extracts of fruits at other stages of ripening. Additionally, we found that the reduction of flavonoid content occurs because of decreased transcriptional levels of genes involved in flavonoid biosynthesis pathway during the ripening process. Based on HPLC analysis, we found that the extract of unripe fruits contained the highest amount of myricetin, caffeic acid, chlorogenic acid, syringic acid, and p-coumaric acid and suggested that the antioxidant and anti-elastase activities of the extract obtained from stage 1, should be mediated by the presence of these compounds. Additionally, we analyzed the interaction sites and patterns between these compounds and elastase using the structure-based molecular docking approach, and suggested that chlorogenic acid strongly interacted with elastase. Together, these findings suggest that the maturity of fruits has profound effects on the pharmaceutical value of R. rugosa.

• Journal of the Korean Institute of Landscape Architecture
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• v.49 no.1
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• pp.70-82
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• 2021

The purpose of this study is to reveal the tendency of planting design through the analysis of the planting of designer's gardens in the Suncheon Bay National Garden and to derive implications for future garden planting designs. The results of the study are as follows: First, the results of the study show that the practice of tree-based planting is still valid. Large growing trees such as 'Pinus densiflrora', 'Celtis sinensis', 'Zelkova serrata', 'Machilus thunbergii', 'Pinus strobus' overwhelmed the size of the designer's garden(150 to 390㎡). Second, the selection of trees tended to be made considering the designer's intention and the decorative effects rather than by considering the physiological and ecological conditions of the site. Third, among the herbaceous, the rate of the planting of perennials was high. Fourth, the flowering period of planted herbaceous was the most common in summer, followed by spring, fall, and winter. Fifth, the frequency color of the planted herbaceous was the most common in summer, followed by spring, fall, and winter. Fifth, in terms of flower color frequency, the most common was the yellow-series, followed by red-series, blue-series Sixth, average height herbaceous plants(20~60cm) were planted the most(47.4%). Seventh, structural plants that determined the garden's framework depended on trees, and the focal plants mainly utilized were evergreen trees, and the midrange plants were the planted herbaceous plants. The implications derived from the above findings are as follows: First, to ensure the garden's quality and sustainability, the selection of trees should be carefully considered, not considering only the artist's intention but also taking into account the physical and ecological conditions. Second, herbaceous plants can be used in various ways― the garden's focal plants, midrange plants, and ground covers, so more active herbaceous planting needs to be considered. Third, in consideration of the winter landscape, herbaceous planting using characteristics, such as fruits and stems, as well as flower colors should be considered. Fourth, blue and black color herbaceous plants have a noticeable effect even in a small amount, so it is necessary to plant them actively. Fifth, for the design of herbaceous planting, where the individual property of plants can be expressed, the design method should be considered.

• Journal of the Korean Institute of Landscape Architecture
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• v.50 no.1
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• pp.68-77
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• 2022

The human thermal environment in an apartment complex located in Seoul was quantitatively analyzed to devise methods to modify human heat-related stresses in landscape and urban planning. Microclimatic data (air temperature, relative humidity, wind speed, and short- and long-wave radiation) were collected at 6 locations [Apt-center, roof (cement), roof (grass), ground, playground, and a tree-lined road] in the late spring and summer, and the data were used to estimate the human thermal sensation, physiological equivalent temperature (PET) and universal thermal climate index (UTCI). As a result, the playground location had the highest thermal environment, and the roof (grass) location had the lowest. The mean difference between the two locations was 0.8-1.1℃ in air temperature, 1.8-4.0% in relative humidity, and 7.5-8.0℃ in mean radiant temperature. In open space locations, the wind speed was 0.4-0.5 ms-1 higher than others. Also, a wind tunnel effect happened at the Apt-center location during the afternoon. For the human thermal sensation, PET and UTCI, the mean differences between the playground and roof (grass) locations were: 5.2℃ (Max. 11.7℃) in late spring and 5.4℃ (Max. 18.1℃) in summer in PET; and 3.0℃ (Max. 6.1℃) in late spring and 2.6℃ (Max. 9.8℃) in summer in UTCI. The mean differences indicated a level change in PET and 1/2 level in UTCI, and the maximum differences showed greater changes, 2-3 levels in PET, and 1-1.5 levels in UTCI. Moreover, the roof (grass) location gave 4.6℃ PET reduction and a 2.5℃ UTCI reduction in late spring, and a 4.4℃ PET reduction and a 2.0℃ UTCI reduction in the summer when compared with the roof (cement) location, which results in a 2/3 level change in PET and a 1/3 level in UTCI. Green infrastructure locations [roof (grass), ground, and a tree-lined road] were not statistically significant in the reduction of PET and UTCI in thermal environment modifying effects. The implementation of green infrastructure, such as rooftop gardens, grass pavement, and street tree planting, should be adopted in landscape planning and be employed for human thermal environment modification.

• Journal of the Korean Society of Radiology
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• v.16 no.3
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• pp.335-341
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• 2022

The purpose of this study was to investigate the radiation protection effect of blueberries. The experimental animals used in this study were 8-week-old 21 SD male rats weighed 280-300 g. The animals were set to a normal group (A), a 5 Gy control group (B), and a 5 Gy experimental group (C) of seven rats each, and (50 mg/kg/day) of physiological saline solution of blueberries were orally administered twice a day with an oral dose of (200 mg/kg/day) for seven days and 5 Gy of radiation was irradiated in the case of groups B and C. As a result, it was identified that there was significance in white blood cells in this study (p<0.000). There was no significant difference in red blood cells or platelets. When examined in detail, among white blood cells (WBC), neutrocytes were found to be significantly different among the three groups: normal, control, and experimental groups (p<0.004). Lymphocytes were also found to be statistically significantly different among the three groups (p<0.000). Monocytes were not found to be statistically significantly different (p<0.483). When red blood cells (RBC) were examined, hemoglobin (HGB) was not found to be statistically significant different among the three groups (p<0.291). Hematocrit (HCT) was not found to be statistically significantly different among the three groups, either (p<0.564). Mean corpuscular volume (MCV) was found to be statistically significantly different among the three groups (p<0.001). Mean corpuscular hemoglobin (MCH) was also found to be statistically significantly among the three groups (p<0.028). Mean corpuscular hemoglobin concentration (MCHC) was found to be statistically significantly different among the three groups(p<0.020). Red blood cell distribution width (RDW) was not found to be statistically significantly different among the three groups (p<0.09). When platelets (PLT) were examined in detail, mean platelet volume (MpV) was found to be statistically significantly different among the three groups (MpV) (p<0.04). In conclusion, based on this study, blueberries are considered to have radiation protection effects.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.15 no.3
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• pp.140-147
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• 2010

We investigated the effects of water temperature, salinity and irradiance on the growth of the harmful algae Chattonella ovata isolated from South Sea, Korea. C. ovata grew under all combinations of water temperatures and salinity, except for all the salinity conditions at the water temperature of $10^{\circ}C$, with the salinity of 7.5 psu and 10 psu at $15^{\circ}C$, and 7.5 psu at $20^{\circ}C$ and $30^{\circ}C$. The maximum specific growth rate was $0.62\;day^{-1}$ at the combination of $30^{\circ}C$ and 30 psu. The results of two-way ANOVA indicated that growth rate depended greatly on the water temperatures while not being affected by interactions with the salinity. This indicates that C. ovata is a stenothermal and euryhaline organism, preferring high water temperatures. C. ovata did not grow at irradiance ${\leq}30\;{\mu}mol$ photons $m^{-2}s^{-1}$. Photoinhibition did not occur at $800\;{\mu}mol$ photons $m^{-2}s^{-1}$, which was the maximum irradiance used in this study. The irradiance-growth curve was described as $\mu$ = 0.74(I-16.0)/(I+43.9) at $30^{\circ}C$ and 30 psu. The half-saturation light intensity ($K_s$) was $75.9\;{\mu}mol$ photons $m^{-2}s^{-1}$ and compensation photon flux density ($I_c$) was $16.0\;{\mu}mol$ photons $m^{-2}s^{-1}$, especially this value was comparatively lower than those of Skeletonema costatum and other flagellates previously reported. Therefore, our results indicate that C. ovata has advantageous physiological characteristics for interspecific competition at the embayment and coastal areas of Korea in summer.