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http://dx.doi.org/10.9721/KJFST.2021.53.4.423

Protective effect of Eucommia ulmoides oliver leaves against PM2.5-induced oxidative stress in neuronal cells in vitro  

Kim, Min Ji (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Kang, Jin Yong (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Park, Seon Kyeong (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Jong Min (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Moon, Jong Hyun (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Gil Han (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Hyo Lim (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Jeong, Hye Rin (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Heo, Ho Jin (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Food Science and Technology / v.53, no.4, 2021 , pp. 423-433 More about this Journal
Abstract
This study was performed to examine the neuroprotective effect of the ethyl acetate fraction from Eucommia ulmoides oliver leaf (EFEL) on PM2.5-induced cytotoxicity. EFEL had higher total phenolic and flavonoid contents than the other fractions. In ABTS and DPPH radical scavenging activities, the IC50 of EFEL was measured as 212.80 and 359.13 ㎍/mL, respectively. To investigate the neuroprotective effect of EFEL, MTT and DCF-DA assays were performed on HT22, MC-IXC, and BV-2 cells. EFEL effectively decreased PM2.5-induced intercellular reactive oxygen species (ROS) content and inhibited PM2.5-induced cell death. In the results of protein expression related to cellular cytotoxicity on microglial cells (BV-2), EFEL had an improvement effect on cell apoptosis and inflammatory pathways. Rutin and chlorogenic acid were identified as the main physiological compounds. Moreover, it was expected that EFEL, including rutin and chlorogenic acid, could be functional food substances with neuroprotective effects against PM2.5-induced oxidative stress.
Keywords
Eucommia ulmoides oliver leaf; $PM_{2.5}$; oxidative stress; neuroprotection; apoptosis;
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