• Title/Summary/Keyword: Physical Sterilization

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Laparoscopic Salpingectomy for Sterilization in an Asian Black Bear (Ursus thibetanus) (반달가슴곰에서 불임을 위한 복강경 이용 난관 절제술 1례)

  • Yoon, Hun-Young;Chung, Byung-Hyun;Lee, Soo-Han
    • Journal of Veterinary Clinics
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    • v.31 no.2
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    • pp.149-151
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    • 2014
  • A 170 kg, 9-year-old, female Asian black bear was presented for sterilization. Physical examination revealed no evidence of any abnormal clinical sign. Laparoscopic salpingectomy was performed for sterilization. A $25^{\circ}$ laparoscope was inserted in the first port, 1-cm caudal to the umbilical scar, to view the abdominal cavity. Two more ports were made 15 cm caudo-lateral to the first port bilaterally. An atraumatic grasper and a vessel-sealing device were inserted in the bilateral ports. The vessel-sealing device was used to seal and cut the fallopian tube and mesosalpinx. There was no sign of hemorrhage on the severed ends of the fallopian tube and mesosalpinx. At 4 weeks, no physical, behavioral, or other assessable impairment was found. This is the first case report in an Asian black bear to describe laparoscopic salpingectomy for sterilization.

Sterilization of Scoria Powder by Corona Discharge Plasma (코로나 방전 플라즈마를 이용한 화산암재 분말 살균)

  • Jo, Jin Oh;Lee, Ho Won;Mok, Young Sun
    • Applied Chemistry for Engineering
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    • v.25 no.4
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    • pp.386-391
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    • 2014
  • Atmospheric-pressure nonthermal corona discharge plasma was applied to the sterilization of biologically contaminated scoria powder. Escherichia coli (E. coli) culture solution was uniformly sprayed throughout the scoria powder for artificial inoculation, which was well mixed to ensure uniformity of the batch. The effect of the key parameters such as discharge power, treatment time, type of gas and electrode distance on the sterilization efficiency was examined and discussed. The experimental results revealed that the plasma treatment was very effective for the sterilization of scoria powder; 5-min treatment at 15 W could sterilize more than 99.9% of E. coli inoculated into the scoria powder. Increasing the discharge power, treatment time or applied voltage led to an improvement in the sterilization efficiency. The effect of type of gas on the sterilization efficiency was in order of oxygen, synthetic air (20% oxygen) and nitrogen from high to low. The inactivation of E. coli under the influence of corona discharge plasma can be explained by cell membrane erosion or etching resulting from UV and reactive oxidizing species (oxygen radical, OH radical, ozone, etc.), and the destruction of E. coli cell membrane by the physical action of numerous corona streamers.

Efficacy of Listeria Innocua Reduction on Enoki Mushrooms by Utilization of an Air Sterilization Device (공기 살균 장치 적용 팽이버섯 재배사의 Listeria Innocua 저감 효과)

  • Lee, Hyun-Dong;Yu, Byeong-Kee;Seo, Da-Som;Kim, Se-Ri;Lee, Chan-Jung;Kwak, Kang-Su
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.210-215
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    • 2021
  • For sterilization of microorganisms of the Listeria genus contaminating enoki mushroom, pilot mushroom grower equipped with air sterilization devices were developed. Sterilization experiments were performed using physical and chemical treatments. Internal temperature and humidity were controlled, maintaining 6.62℃±0.30 in the upper shelves, 6.46℃±0.24 in the middle shelves, and 6.48℃±0.25 in the lower shelves. Humidities were 79.97%±4.42, 79.43%±4.06, and 79.94±4.30%, respectively, with a temperature setting of 6.5℃, and a relative humidity of 75%. A suitable enoki mushroom cultivation stage for air sterilizer application was during the growth stage, with temperature in the 6.5~8.5℃ range, and humidity of 70~80%. At these same internal conditions, the ozone concentration in the mushroom cultivator was found to be 160 ppb during ion-cluster generator operation. After physical sterilization, the Listeria innocua survival rate was 0.1 to 0.9% using ion cluster sterilization, and 9.3 to 10.6% using UV air sterilization. The Listeria innocua survival rates on different materials were 9.3~10.6% on the metal specimen, and 9.9~16.2% on the plastic wrapper. The survival rate was particularly high on the rough side of the plastic wrapper. Ion cluster air sterilization is a labor-saving and effective method for suppressing the occurrence of Listeria bacteria on mushroom growers walls and shelves. For the plastic wrapper, chemical sterilization is more effective than physical sterilization.

AN EXPERIMENTAL STUDY ON THE FREEZE-DRIED BONE OF MAXILLA AND MANDIBLE IN KOREANS ;I. A microbiologic study of the sterility on the fresh-frozen and freeze-dried bone of human (동결 건조한 한국인 상하악골에 대한 실험적 연구;I. 단순냉동 및 냉동 건조된 동종골의 멸균에 관한 실험적 연구)

  • Um, In-Woong
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.13 no.4
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    • pp.405-411
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    • 1991
  • To determine the sterility of the prepared allogeneic bone of the human, culture of the allografts prior to implantation was performed on fresh-frozen and freeze-dried bone. Before the use of allografts to the patients, it must be confirmed about the sterility, cellular cytotoxicity, immune reaction, and osteoinductive potential as a biomaterials. Oral and maxillofacial surgeons demand for allograft bone will be increased in the future. Wonkwang Bone Bank attempted to meet this demand, has performed series of experimental study on the allograft bone of the Koreans to evaluate the physical and chemical suitability of the bone since the surgeons applications will have broadened from benign cystic lesions to fracture malunions and non-unions, large segmental defects, and whole-bone allorgrafts after tumor surgery. The results obtained were as follows: 1. Freeze-drying(FD) only shoed some bactericidal effects of the normal and osteo bone but in cases of performing EO gas sterilization, the FD effects was not clear. 2. The fact that FD has little effect than the EO gas sterilization on normal bone postulated that the presence of microbiota may be due to an operation and bone processing procedure. 3. FD and EO gas sterilization had a remarkable effect on the osteo bone. 4. The sterilization effect were EO gas, Freeze-drying, Fresh-Frozen with descending order. But all sterilization method were not complete to preserve and implant allograft bone. We are now performing further continuous study on the radiation and chemical sterilization procedure to make safe and complete allograft bone.

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A COMPARATIVE STUDY ON THE PHYSICAL PROPERTIES OF ORTHODONTIC PLIERS ACCORDING TO TYPES OF STERILIZATION (멸균 방법에 따른 교정용 플라이어의 물성 변화에 대한 비교 연구)

  • Cho, Il-Je;Son, Woo-Sung
    • The korean journal of orthodontics
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    • v.28 no.2 s.67
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    • pp.329-341
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    • 1998
  • Sterilization has received much attention in orthodontic practices over the past several years. The present study was undertaken to investigate the effects of sterilization on the physical properties of orthodontic pliers-AEZ, Unitek, and Dentronix ligature cutters. This study was designed to examine the tips of ligature cutters before and after 200 and 400 sterilization cycles using the Bowmar RHT-1000, the Dentronix DDS-5000, and the Eschmann SES-2000. The tip surface and the fracture surface were observed with a scanning electron microscope. The microstructure was observed with an optical microscope. The hardness test was carried out with the mic개-Vickers hardness tester and the Rockwell C Scale hardness tester. The chemical composition was analyzed by energy dispersive X-ray spectrometer. The results of this study were as follows : 1. The number and the size of corrosion products on the tip surface and the proportion of cleavage planes in fractured specimen increased, but the hardness of the tip decreased in proportion to sterilization cycles. From these observations, it was considered that mechanical properities decreased in proportion to sterilization cycles. 2. The number and the size of chromium carbides increased in proportion to sterilization cycles. Coarse microstructure decreased mechanical properities. 3. The AEZ and Unitek ligature cutters were Fe-Cr stainless steels, but the Dentronix ligature cutter was Co-Cr alloy. There were many differences among manufactures, but the chemical composition was .not changed after sterilization cycles. 4. The tip edge of ligature cutter used in a clinic revealed microcracks with the SEM observation. Clinical experience confirmed that ligature cutters were gradually degraded by sterilization.

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Removing Resin Contaminants from the Seated Stone Buddha (Korean Treasure No.84) of Sinboksa Temple Site in Gangneung (강릉 신복사지 석조보살좌상 보물 제84호 수지상 오염물 제거 방안 연구)

  • Lee, Byeonghoon;Go, Hyeongsun
    • Conservation Science in Museum
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    • v.15
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    • pp.56-65
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    • 2014
  • The face of the Seated Stone Buddha Korean Treasure No.84 of Sinboksa Templesite in Gangneung was contaminated with a glossy resin, spoiling its beauty. The contaminants covered the face, the forehead of the urna, to the bottom of the jaw. FT-IR analysis on a sample of the contaminants identified the substance as cyanoacrylate instant adhesive, which can normally be melted with acetone and removed with a swab. However, given that the surface of the statue was severely weathered from prolonged open-air exposure, alternative removal procedures were considered, to minimize the possibility of physical damage. After research, two removal procedures were utilized: one procedure involving poultices, which had been used to remove graffiti from the Samjeondobi Stele, and one procedure involving steam sterilization, which has been used to remove resin from pottery and porcelain. When both the poultices and steam sterilization were applied together, the resin was removed within twenty-four hours, without physical work using a swab.

A Study on the Sterilization Effect of Ballast Water according to the Combination of Types of Treatment Apparatus (선박평형수 처리장치의 조합에 따른 살균효과에 관한 연구)

  • Kang, Ah-Young;Kim, Sang-Pil;Song, Ju-Yeong
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.3
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    • pp.412-417
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    • 2015
  • The purpose of this study is to treat the ballast water by shear stress without an environmental pollution and to find out the optimal treatment conditions. The ballast water problem is issued up as the trade activated and the cargos mobilized. To improve this problem, International Marine Organization(IMO) make the rule about the ballast water treatment with specific restrictions. Although many countries have been studying about the ballast water treatment technology, there is almost no technology that can treat the microorganisms under $50{\mu}m$ without any secondary pollution. In this study, we tried to treat ballast water by applying shear stress as the physical treatment for the sterilization and tried to find out the optimal conditions including the 100% sterilizing rate and the best economic condition.

A Comparison Study on the Sterilization Effect of Ballast Water with Cylinder Type and Groove Type (선박평형수 처리장치의 cylinder type과 groove type의 살균효과 비교 연구)

  • Kang, Ah-Young;Kim, Sang-Pil;Kim, Young-Cheol;Song, Ju-Yeong
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.4
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    • pp.616-622
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    • 2015
  • Current ballast water treatment technologies are applying chemical or electrical treatment technology which are not free from secondary environmental pollution. The purpose of this study is to treat the ballast water by shear stress without an additional environmental pollution and to find out the optimal treatment apparatus. We tried to treat ballast water by applying shear stress with two different type of combination of inner and outer cylinder, such as non-pattern type and groove type. In the case of non-pattern type of inner and outer cylinder, sterilization effect was comparatively low because of a slip between inner and outer cylinder. But in the case of groove type of inner and outer cylinder, sterilization effect was superior to the non-pattern type. With a same revolutional speed of 8000rpm, an extinction effect was acquired in the gap of 1 mm of inner and outer cylinder at non-pattern type, but 3mm of that of groove type.

Protocol for Disinfection and Sterilization in Dental Clinic (임상가를 위한 특집 1 - 멸균, 소독 및 감염 관리 점검 방안)

  • Cha, Su Ryeon;Kim, Kang-Ju
    • The Journal of the Korean dental association
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    • v.51 no.3
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    • pp.130-137
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    • 2013
  • Infection control is critical to good dental practice. To this end, it is essential that the practitioner not only understand the concepts involved in the development and applications of antimicrobial(physical and chemical) agents but also know how to use them properly in the dental operatory. Practical applications of infection control are more fully described in several of the suggested references.

The Effects of Edible Coating and Hurdle-Technology on Quality Maintenance and Shelf-Life Extension of Seafood (식용 코팅 및 허들기술이 수산물의 품질 유지와 저장성 연장에 미치는 영향)

  • Baek, Ji Hye;Lee, So-Young;Oh, Se-Wook
    • Journal of Food Hygiene and Safety
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    • v.35 no.3
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    • pp.205-212
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    • 2020
  • Foodborne diseases occur frequently and have various being related to the intake of contaminated foods. Seafood products are susceptible to contamination due to higher water content and microorganisms, which combine to give them a short shelf-life. Various approaches have been applied to overcome this problem. Edible coatings that are also biodegradable and biocompatible have been discussed as one of the applicable solutions. These coatings can actually help to maintain seafood quality by inhibiting the growth of microorganisms and delaying the loss of moisture. This paper presents the effects of various natural bio-polymers, antimicrobial substances and physical sterilization techniques such as gamma irradiation, ultraviolet (UV) sterilization, and light-emitting diode (LED) sterilization on seafood coatings.