• Asian-Australasian Journal of Animal Sciences
• /
• 제29권4호
• /
• pp.471-478
• /
• 2016

Native duck populations have lower productivity, and have not been developed as much as commercials duck breeds. However, native ducks have more importance in terms of genetic diversity and potentially valuable economic traits. For this reason, population discriminable genetic markers are needed for conservation and development of native ducks. In this study, 24 highly polymorphic microsatellite (MS) markers were investigated using commercial ducks and native East and South Asian ducks. The average polymorphic information content (PIC) value for all MS markers was 0.584, indicating high discrimination power. All populations were discriminated using 14 highly polymorphic MS markers by genetic distance and phylogenetic analysis. The results indicated that there were close genetic relationships among populations. In the structure analysis, East Asian ducks shared more haplotypes with commercial ducks than South Asian ducks, and they had more independent haplotypes than others did. These results will provide useful information for genetic diversity studies in ducks and for the development of duck traceability systems in the market.

• Mycobiology
• /
• 제43권3호
• /
• pp.231-238
• /
• 2015

A total of 4 aquatic plants, Eleocharis kuroguwai Ohwi, Hydrocharis dubia Backer, Salvinia natans All., and Zizania latifolia Turcz., were sampled from representative two wetlands of South Korea. A total of 38 endophytic fungal strains were isolated from aquatic plants native to the Daepyeong wetland, and 27 strains were isolated from the Jilnal wetland. The internal transcribed spacer regions of fungal isolates were sequenced and a phylogenetic analysis was performed. In addition, endophytic fungal diversity from each wetland and host plant species was deduced. A total of 25 fungal genera were purely isolated, and 16 fungal genera were isolated from each of the two wetlands. Commonly isolated genera from both wetlands were Aspergillus, Cladosporium, Clonostachys, Fusarium, Leptosphaeria, Penicillium, and Talaromyces. This study revealed that fungal diversity varied with environmental conditions and by host plant in representative two wetlands.

• Plant Breeding and Biotechnology
• /
• 제5권3호
• /
• pp.213-220
• /
• 2017

Production of spring wheat, the major crop in Mongolia, accounts for 98% of the cultivated area. Understanding genetic variability in existing gene bank accessions is critical for collection, conservation and use of wheat germplasms. To determine genetic diversity and population structure among a representative collection of Mongolian local wheat cultivars and lines, 200 wheat accessions were analyzed with 15 SSR markers distributed throughout the wheat genome. A total of 85 alleles were detected, with three to five alleles per locus and a mean genetic richness of 5.66. Average genetic diversity index was 0.69, with values ranging from 0.37-0.80. The 200 Mongolian wheat accessions were mainly divided into two subgroups based on structure and phylogenetic analyses, and some phenotypes were divergent by the subgroups. Results from this study will provide valuable information for conservation and sustainable use of Mongolian wheat genetic resources.

• The Plant Pathology Journal
• /
• 제37권6호
• /
• pp.512-520
• /
• 2021

In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.

• Mycobiology
• /
• 제50권5호
• /
• pp.302-316
• /
• 2022

Many Apiospora species have been isolated from bamboo plants - to date, 34 bambusicolous Apiospora species have been recorded. They are known as saprophytes, endophytes, and plant pathogens. In this study, 242 bambusicolous Apiospora were isolated from various bamboo materials (branches, culms, leaves, roots, and shoots) and examined using DNA sequence similarity based on the internal transcribed spacer, 28S large subunit ribosomal RNA gene, translation elongation factor 1-alpha, and beta-tubulin regions. Nine Apiospora species (Ap. arundinis, Ap. camelliae-sinensis, Ap. hysterina, Ap. lageniformis sp. nov., Ap. paraphaeosperma, Ap. pseudohyphopodii sp. nov., Ap. rasikravindrae, Ap. saccharicola, and Ap. sargassi) were identified via molecular analysis. Moreover, the highest diversity of Apiospora was found in culms, and the most abundant species was Ap. arundinis. Among the nine Apiospora species, two (Ap. hysterina and Ap. paraphaeosperma) were unrecorded in Korea, and the other two species (Ap. lageniformis sp. nov. and Ap. pseudohyphopodii sp. nov.) were potentially novel species. Here, we describe the diversity of bambusicolous Apiospora species in bamboo organs, construct a multi-locus phylogenetic tree, and delineate morphological features of new bambusicolous Apiospora in Korea.

• Mycobiology
• /
• 제50권5호
• /
• pp.326-344
• /
• 2022

The fungal-like family Saprolegniaceae (Oomycota), also called "water mold," includes mostly aquatic saprophytes as well as notorious aquatic animal pathogens. Most studies on Saprolegniaceae have been biased toward pathogenic species that are important to aquaculture rather than saprotrophic species, despite the latter's crucial roles in carbon cycling of freshwater ecosystems. Few attempts have been made to study the diversity and ecology of Saprolegniaceae; thus, their ecological role is not well-known. During a survey of oomycetes between 2016 and 2021, we investigated the diversity and distribution of culturable Saprolegniaceae species in freshwater ecosystems of Korea. In the present study, members of Saprolegniaceae were isolated and identified at species level based on their cultural, morphological, and molecular phylogenetic analyses. Furthermore, substrate preference and seasonal dynamics for each were examined. Most of the species were previously reported as animal pathogens; however, in the present study, they were often isolated from other freshwater substrates, such as plant debris, algae, water, and soil sediment. The relative abundance of Saprolegniaceae was higher in the cold to cool season than that in the warm to hot season of Korea. This study enhances our understanding of the diversity and ecological attributes of Saprolegniaceae in freshwater ecosystems.

• The Plant Pathology Journal
• /
• 제40권5호
• /
• pp.551-558
• /
• 2024

Papaya leaf curl China virus (PaLCuCNV) is a damaging plant pathogen causing substantial losses to crop. The complete genomes of three PaLCuCNV isolates from Ageratum conyzoides were obtained and combined with the 68 reference isolates in GenBank for comprehensive genetic diversity analyses using specialized computational tools. Sequence alignment revealed nucleotide sequence similarity ranging from 85.3% to 99.9% among 71 PaLCuCNV isolates. Employing phylogenetic analysis, 71 PaLCuCNV sequences were clustered into five groups, with no significant correlation observed between genetic differentiation and either host species or geographical origin. Additionally, 13 recombination events across all PaLCuCNV isolates were identified. Genetic diversity analysis indicated the ongoing expansion and evolution of PaLCuCNV populations, supported by a neutral model. Moreover, significant genetic differentiation was observed among distinct viral populations, primarily attributed to genetic drift. Overall, our findings provide valuable insights into the detection, genetic variation, and evolutionary dynamics of PaLCuCNV.

• 식물분류학회지
• /
• 제46권2호
• /
• pp.247-255
• /
• 2016

천주교 대구교구청에 심어져 있는 오래된 왕벚나무의 기원을 추적하기 위하여 제주도에 자생하는 야생 왕벚나무와 재배 왕벚나무(Somei-yoshino cherry)의 계통분류학적 유연관계를 알아보았다. 한국과 일본에서 채집한 야생 왕벚나무, 재배 왕벚나무 및 근연종인 올벚나무, 총 25 개체에 대하여 cpDNA 두 구간(rpl16 유전자, trnS-trnG intergenic spacer)의 염기서열을 사용하여 계통수와 반수체형(haplotype) 네트워크를 작성하여 두 분류군을 비교하였다. 야생 왕벚나무와 재배 왕벚나무는 서로 구별되는 분류군으로 드러났으며, 비록 적은 샘플을 대상으로 비교적 짧은 유전자위가 사용되었지만 야생 왕벚나무는 재배 왕벚나무보다 반수체형 다양성이 높은 것으로 나타났다. 이는 야생 왕벚나무의 교배 기원에 모계쪽으로 기여한 것으로 알려진 올벚나무의 유전적 다양성에서 기인하는 것으로 추정된다. 따라서, 야생 왕벚나무와 재배 왕벚나무의 계통분류학적 관계를 보다 명확하게 파악하기 위하여 올벚나무를 한국과 일본의 다양한 분포 지역에서 넓게 채집하여 추가 연구를 실시할 필요가 있다고 생각된다. Taquet 신부가 제주에서 채집하여 대구에 옮겨 심었다고 추정되었던 천주교 대구교구청의 오래된 왕벚나무는 야생 왕벚나무가 아닌 재배 왕벚나무로 보는 것이 타당하다.

• 한국산림과학회지
• /
• 제95권4호
• /
• pp.388-392
• /
• 2006

국내에 분포하는 리지나뿌리썩음병균(Rhizina undulata)의 생리적 특성 및 유전적 다양성을 밝히기 위하여, 소나무(Pinus densiflora) 및 곰솔(P. thunbergii) 림으로부터 분리한 13종의 리지나뿌리썩음병균 분리주를 공시하여 각 분리주들의 배양 특성 및 RAPD에 의한 유전적 다양성을 분석하였다. P. densiflora 및 P. thunbergii로부터 제조한 수용성 추출물 첨가배지에서의 각 분리주들의 균사생장 특성을 조사한 결과, 각 분리주들의 기주와 분리주들의 기주로 부터 추출한 수용성 추출물 배지에서의 균사생장량 간에는 상관관계를 발견할 수 없었다. 한편, 12종의 random primer를 사용하여 R. undulata 분리주들의 genomic DNA의 random amplified polymorphic DNA(RAPD)에 의한 유전적 다양성을 분석한 결과, 국내 분리주들의 RAPD profile은 모두 동일하였다. 그러나, 국내 분리주들의 RAPD profile과 일본 분리주와는 다소 차이를 나타내었는데 즉, RAPD profile의 phylogenetic tree 분석 결과, 국내 분리주들과 일본분리주와는 88%의 상동성을 나타내었다.

• 식물병연구
• /
• 제24권4호
• /
• pp.285-291
• /
• 2018

국내육성딸기 품종인 설향과 감홍에서 딸기누른오갈바이러스의 국내 분리주 2종을 분리하고 외피단백질 전체 염기서열을 결정하고 분석하였다. 국내 분리주 SH와 KH의 외피단백질 염기와 아미노산 상동성은 각각 90.4%와 95.5% 였다. 기존에 국내에서 보고된 KNS1분리주와 GenBank에 등록된 45개의 다른나라 분리주 외피단백질 염기서열을 모두 수집하여 총 48개 SMYEV 외피단백질에 대한 계통학적 유연관계를 분석할 결과 총 5개의 subgroup (I-V)으로 분류가 되었다. 이 중 subgroup IV과 V과 새로운 변이집단으로 국내분리주도 KH와 KNS1은 subgroup I에 포함된 반면, SH는 새로운 subgroup인 IV에 포함되어 국내분리주간에도 계통이 다른 것을 추측할 수 있었다. 유전적 다양성 분석결과 SMYEV의 새로운 subgroup의 다양성이 더욱 높은 것으로 나타나 SMYEV가 유전적으로 진화를 하고 있음을 알 수 있었다. 이 논문은 국내 SMYEV 분리주에 대한 분자적 특성에 대한 첫 보고이다.