• 제목/요약/키워드: Photosynthetic

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Universal Existence of One Chlorophyll a' Molecule in Photosystem I of Oxygenic Photosynthetic Organisms

  • Nakamura, Akimasa;Yoshida, Emi;Taki, Takashi;Watanabe, Tadashi
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.367-369
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    • 2002
  • Chlorophyll (ChI) a' is the Cl3$^2$-epimer of ChI a which is the constituent of P700, the primary electron donor of Photosystem (PS) I, of a thrmophilic cyanobacterium, Synechococcus elongatus, whose structure was recently determined by X-ray crystallography. To determine whether PS I of diverse oxygenic photosynthetic organisms universally contain one molecule of ChI a ’, pigment compositions of thylakoid membranes and PS I complexes isolated from cyanobacteria, green algae, red algae and higher plants were determined by reversed-phase HPLC. The results show that involvement of one ChI a'molecule in PS I is the universal feature for Chi a-based PS I of oxygenic photosynthetic organisms.

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Direct Triazine Herbicide Detection Using a Self-Assembled Photosynthetic Reaction Center from Purple Bacterium

  • Nakamura, Chikashi;Hasegawa, Miki;Shimada, Kazumi;Shirai, Makoto;Miyake, Jun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.6
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    • pp.413-417
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    • 2000
  • In this study, a direct detection system for triazine derivative herbicides was developed using the photosynthetic reaction center (RC) from the purple bacterium, Rhodobacter sphaeroides, and surface plasmon resonance (SPR) apparatus. The histidine-tagged RCs were immobilized on an SPR gold chip using nickel-nitrilotriacetic acid groups as a binder for one of the triazine herbicide, atrazine. The SPR responses were proportional to the sample concentrations of atrazine in the range 0.1-1 $\mu\textrm{g}$/mL. The sensitivity of the direct detection of atrazine using the RC-assembled sensor chip was higher than that using the antibody-immobilized chip. The other types of herbicides, DCMU or MCPP, were not detected with such high sensitivity. The results indicated the high binding selectivity of the RC complex.

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Studies on the Characters of Green-stem Varient Ginseng Plant (청경종의 제형질 특성에 관하여)

  • 천성룡;김경태
    • Journal of Ginseng Research
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    • v.13 no.2
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    • pp.153-157
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    • 1989
  • The characteristics of the areal parts, photosynthetic and respiration rates and stem color were examined in 1- to 5-year-old Green-stem, Violet-stem and Yellow-berry variant ginseng (Panax ginseng C.A. Meyer). The top characteristics, photosynthetic and respiration rates were not significantly different among the three variants. Selfed progency of the Green-stems variant plant produced only green stems, while the others produced green and violet stem in the seedling stage. These results showed that most of the top characteristics of the Green-stem variant were similar those of the Violet-stem and Yellow-berry variants with age.

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Reaction Characterization of a Photosynthetic Bacterium Containing $Q_{10}$ ($Q_{10}$ 함유 광합성세균의 반응특성)

  • Jeong, Soo Kyoung;Kim, Joong Kyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.3
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    • pp.198-200
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    • 2007
  • Cultivation of a $Q_{10}$-producing photosynthetic bacterium, Rhodobacter sphaeroids, was carried out in a 1-L flask to characterize its cellular growth reaction. The result of experiment showed that dissolve oxygen in the broth was depleted within 7 h. ORP decreased with decrease of DO, and recovered somewhat with increase of pH. The growth of R. spahaeroids reached at late-log phase in 140 h of reaction. The final pH and dry-cell weight were 7.62 and 2.2 mg/mL, respectively. The $Q_{10}$ content in the final broth was 2.35 mg/g dry cell weight, which was higher than that obtained in tube culture.

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Gender Variation in Relation to Resource Allocation in Ambrosia artemisiifolia (Ambrosia artemisiifolia에 있어서 자원분배와 관련된 성비의 변이)

  • Kang, Hyesoon
    • The Korean Journal of Ecology
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    • v.18 no.2
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    • pp.231-241
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    • 1995
  • I examined the relationship between allocations to plant size components such as plant height, total leaf weight, and nuber of branches and to reproductive components such as the number and weight of male and female fowers in three populations of the monoecious, wind-pollinated Ambrosia artemisiifolia. In particular, the two types of gender of plants, phenotypic and functional gender, were related to resource allocation. Mean values of all plant size and reproductive components differed significantly among the three populations. Such inter-population differences did not exist for both photosynthetic and reproductive efforts, Plant size components were mostly positively correlated with reproductive components among plants. However, the relationships between photosynthetic effort and reproductive components were largely negative and inconsistent among populations. The phenotypic gender was much lover than 0.5 in all of the three populations. Pairwise correlations between functional gender and each allocation measurement such as plant size components, reproductive components, photosynthetic effort, and reproductive effort were not consistent across the three populations. These results suggest that the gender of A. artemisiifolia measured as flower production responds sensitively to environmental conditions which might differ whithin as well as among populations.

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Competitive Performance of Hybrid Rice with Barnyardgrass

  • Lin, Wenxiong;Kim, Hak-Yoon;Shin, Dong-Hyun;Lee, In-Jung;Kim, Kil-Ung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.4
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    • pp.391-395
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    • 1999
  • Barnyardgrass had relatively higher growth vigor at the earlier growth stage than inbred rice did, showing the 2 fold higher $\alpha$-amylase activity during the periods of germination and large leaf area Expansion with high net photosynthetic rate at the earlier autotrophic stage, but it performed weak growth at the late growth stage. However, the hlybrid rice Shanyou 63 had significantly higher $\alpha$-amylase activity and net photosynthetic rate than that of barnyardgrass, exhibiting heterosis for two physiologica1 traits during the germination (6~12 days) and autotrophic phase, respectively. Accordingly, hybrid rice, Shanyou 63, exhibited heterotic effect at the early growing stage when were presented with barnyardgrass. Shanyou 63 exhibited stronger tillering ability, faster leaf area expansion and higher net photosynthetic rate than those of barnyardgrass.

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Efects of Chitosan on Cell Flocculation in Soybean Curd Wastewater Treated by Photosynthetic Bacteria (Chitosan에 의한 광합성세균 처리 두부공업폐수의 균체 응집효과)

  • 오준현;조홍연;양한철
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.763-769
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    • 1995
  • As a mean to recover photosynthetic bacterial(PSB) cells and its practical uses in food industrial wastewater treatment, various biodegradable polyelectrolytes were first investigated for flocculation of suspended colloids in the PSB treatment process of soybean curd wastewater. Anionic polyelectrolytes such as sodium alginate and carrageenan were not effective but a cationic polyelectrolyte chitosan isolated from Portunus trituberclatus showed very effective flocculation activity. The concentration of chitosan, pH and temperature of wastewater for maximal flocculation were 40 mg/l, pH 7 and room temperature, respectively. Test using deacetylated chitosan to various degree showed higher flocculating activities in samples deacetylated over 75% and time for maximum flocculation was 40 min by stirring slowly under the above optimal conditions. Chitosan was not only effective to flocculate cells but also removed COD and MLSS of the wastewater. COD of 42% and MLSS of 87% were removed by addition of chitosan to the soybean curd wastewater treated with PSB.

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Effect of Foliar Treatment of KCl on Chlorophyll, Total Sugars, Soluble Protein, In Vivo Nitrate Reductase Activity and Leaf Yield in Mulberry (Morus alba L. CV.S1)

  • Das, C.;Ghosh, M.K.;Das, B.K.;Misra, A.K.;Mukherjee, P.K.;Urs, S.Raje
    • International Journal of Industrial Entomology and Biomaterials
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    • v.7 no.1
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    • pp.45-49
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    • 2003
  • Foliar treatment with different concentrations of potassium chloride (KCl) to mulberry plants resulted in higher level of total chlorophyll, total sugars, soluble protein, in vivo nitrate reductase activity (NRA), net photosynthetic rate (NPR), pWUE and leaf yield. Optimal concentration was found to be 10.0 mM KCl with limited irrigation provided in the mulberry plantation planted in 90 ${times}$ 90 cm spacing. The deleterious effect of soil moisture stress condition has been found to be overcome by KCl foliar spray twice at 15 days interval. Regression and correlation coefficients were analyzed, and a strong positive correlation was found between chlorophyll and total sugars, soluble protein and in vivo nitrate reductase activity, leaf dry weight and net photosynthetic rate and pWUE and net photosynthetic rate.

Inhibitory Effect of Simazine on Photosynthetic Electron Transport Activity in Anabaena inequalis (Anabaena의 광합성 전자전달 활성에 미치는 Simazine의 억제효과)

  • 권벽동
    • Journal of Plant Biology
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    • v.31 no.3
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    • pp.217-226
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    • 1988
  • Effects of simazine [2-chloro-4,6-bis(methylamino)-s-triazine] on the photochemical reactions of isolaed spinach chloroplasts and crude thylakoids of Anabaena inequalis UTEX B-381 were compared. Simazine inhibited photosynthetic O2 evolution and increased the chlorophyll fluorescence in whole cells of Anabaena. The electron transfer from diphenylcarbazide to 2,6-dichlorophenolindophenol was inhibited by simazine treatment in spinach chloroplasts, but not in crude thylakoids of Anabaena. In spinach chloroplasts, the chlorophyll fluorescence was increased by simazine treatment in the presence of diphenylcarbazide and ferricyanide, but not in the presence of diphenylcarbazide and silicomolybdate. In crude thylakoids of Anabaena, simazine treatment did not increase the chlorophyll fluorescence in the presence of either diphenylcarbazide and silicomolybdate, or diphenylcarbazide and ferricyanide. There results suggest that the inhibitory site of simazine on photosynthetic electron transport chain of anabaena is different from that of spinach chloroplasts. And there may be a possiblity that the inhibition site of simazine in Anabaena lies on the donor side of photosystem II, before the site of electron donation by diphenylcarbazide.

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Anatomical Differentiation and Photosynthetic Adaptation in Brown Algae

  • Garbary, David J.;Kim, Kwang-Young
    • ALGAE
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    • v.20 no.3
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    • pp.233-238
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    • 2005
  • The photosynthetic parameters of dark- adapted minimum fluorescence (Fo) and maximum quantum yield of charge separation in PSII (Fv/Fm) were measured in transverse sections of eight species of marine Phaeophyceae (species of Laminariales, Fucales, Desmarestiales, Chordariales) using pulse amplified modulation (PAM) fluorometry. Within each transverse section fluorescence was measured in three regions corresponding to outer cortical and meristoderm cells, inner cortical cells and innermost medullary cells. Minimum fluorescence declined from 19-74% (mean of 39%) from outermost to innermost cells. Maximum quantum yield varied from 0.51-0.59 in outermost cell layers and this was reduced to 0.23-0.40 in innermost cell layers, with an average reduction of 50%. Despite the reduction Fo in medullary cells (inner), medullas of all species showed maximum quantum yields consistent with a photosynthetic role in carbon fixation. These results show that medullary cells of complex brown algae have more than a role in structure, storage or transport, and may also provide an important role in carbon fixation.