• Journal of Biosystems Engineering
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• v.24 no.2
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• pp.115-122
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• 1999

Because of their small mass, volume, solid state construction and long life, light-emitting diodes(LEDs) hold promises as a lighting source for intensive plant production system. Spectral characteristics and light intensity of LEDs were tested to investigate their feasibility as artificial lighting sources for growth and morphogenesis control in transplant production system. Blue, green, and red LEDs had a peak-emission wavelength at 442nm, 522nm, and 673nm, respectively. Their half width defined as the difference between upper and lower wavelength in the intensity equivalent to 50% of the maximum intensity showed 26nm, 41nm, and 74nm, respectively. Photosynthetic photon flux(PPE) at the distance of 9cm under the LEDs array was measured as $235{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for red, $109{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for green, and $75{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for blue LEDs. At the same distance, green LEDs had the illuminance of 13,0001x, nine to ten times higher than those of red and blue LEDs. Red, green, and blue LEDs at a distance of 9cm had the irradiance of $46W{\cdot}m^{-2},\;19W{\cdot}m^{-2},\;8W{\cdot}m^{-2}$, respectively. Light intensity of blue, green, and red LEDs increased linearly in proportion to the magnitude of the current applied to the operating circuit. Thus the light intensity of LEDs was controlled by the applied current in operating circuit.

• Horticultural Science & Technology
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• v.35 no.4
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• pp.439-445
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• 2017

Transplant production in a plant factory with artificial lighting provides several benefits; (1) rapid and uniform transplant production, (2) high production rate per unit area, and (3) production of disease free transplants production. To improve the growth of runner plants when strawberry transplants are produced in a plant factory, we conducted two experiments to investigate (1) the effect of different light intensity for stock and runner plants on the growth of runner plants, and (2) the effect of different container volume for runner plants on their growth. When the stock and runner plants were grown under nine different light conditions composed of three different light intensities (100, 200, and $400{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPF) for each stock and runner plants, increasing the light intensity for stock plants promoted the growth of runner plants, however, the growth of runner plants was not enhanced by increasing the light intensity for runner plants under same light intensity condition for stock plants. We also cultivated runner plants using plug trays with four different container volumes (21, 34, 73, and 150 mL) for 20 days after placing the stock plants, and found that using plug trays with lager container volume did not enhance the growth of runner plants. These results indicate that providing optimal condition for stock plants, rather than the runner plants, is more important for increasing the growth of the runner plants and that the efficiency of strawberry transplant production in a plant factory can be improved by decreasing light intensity or container volume for runner plants.

• Journal of Biosystems Engineering
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• v.21 no.4
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• pp.429-435
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• 1996

A wind tunnel consisting of two air flow conditioners with polycarbonate pipes, a plant growth room, a suction fan and fan controller, and fluorescent lamps, was designed to investigate the interactions between the growth of plug seedlings under artificial light and their Physical environments. Light transmissivities in the plant growth room based on the photosynthetic photon flux density and photosynthetically active radiation was appeared to be 96.3% and 96.8%, respectively. Measurement showed a uniformity in the vertical profiles of air current speed at the middle and rear regions of plug trays in wind tunnel. This result indicated that the development of a wind tunnel based on the design criteria of the American Society of Mechanical Engineers was adequate. Air current speed inside the plug stand was significantly decreased due to the resistance by the leaves of plug seedlings and boundary layer developed over and below the plug stand. Driving force to facilitate the diffusion of gas inside the plug stand might be regarded as extremely low. Aerodynamic characteristics above the plug stand under artificial light were investigated. As the air current speed increased, zero plane displacement decreased but roughness length and frictional velocity increased. Zero plane displacement linearly increased with the average height of plug seedlings. The wind tunnel developed in this study would be useful to investigate the effects of air current speed on the microclimate over and inside the plug stand and to collect basic data for a large-scale plug production under artificial light in a semi-closed ecosystem.

• ALGAE
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• v.20 no.2
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• pp.121-125
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• 2005

The modern integrated fish-seaweed mariculture has been tested to reduce the environmental impacts of an intensive fed culture. To obtain the best seaweed bioremediation performance, the effects of therapeutants used for fish disease control on the selected seaweed species should be considered. As a selected seaweed, Porphyra yezoensis was tested with six commercial antibiotics including erythromycin thiocyanate_A, erythromycin thiocyanate_B, oxytetracycline, doxycycline, pefloxacin, and amoxicillin trihydrate under the batch incubation at a photon flux density of 10 $\mu$mol ${\cdot}m^{-2}\;{\cdot}\;s^{-1}$ at 15$^{\circ}C$. Among the tested commercial antibiotics, erythromycin thiocyanate_A, erythromycin thiocyanate_B, oxytetracycline, and doxycycline showed decreases in Fv/Fm, the photochemical efficiency of photosystem II, with a dose-dependant and time-dependant manner. From the quenching analysis of chlorophyll fluorescence, three differential patterns were observed in the antibiotics-treated Porphyra: (1) high nonphotochemical quenching (NPQ) and low photochemical quenching (qP) in the cases of Erythromycin thiocyanate_B and amoxicillin trihydrate, (2) high NPQ and high qP in the case of pefloxacin and (3) low NPQ and low qP in the case of oxytetracycline. These results indicated that antibiotics affected in various ways on the photosynthetic apparatus, reflecting differential lesion sites of antibiotics. In addition, the rates of ammonium uptake also decreased with a decrease of Fv/Fm in P. yezoensis thalli treated with erythromycin thiocyanate_B and oxytetracycline. Therefore, the four antibiotics mentioned could affect the bioremediation capacity of the selected seaweed species in the integrated fish-seaweed mariculture system due to the decrease of photosynthetic activity and the simultaneous decrease of ammonium uptake.

• Journal of Environmental Science International
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• v.22 no.12
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• pp.1571-1577
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• 2013

The effects of irradiance on the growth of toxic dinoflagellates Alexandrium tamarense (Masan Bay strain) and Alexandrium catenella (Jinhae Bay strain) were investigated in the laboratory. At $15^{\circ}C$ and 30 psu for A. tamarense and $25^{\circ}C$ and 30 psu for A. catenella, the irradiance-growth curve showed the maximum growth rate (${\mu}_{max}$) of 0.31 $day^{-1}$ with half-saturation photon flux density (PFD) ($K_I$) of 44.53 ${\mu}molm^{-2}s^{-1}$, and a compensation PFD ($I_c$) was 20.67 ${\mu}molm^{-2}s^{-1}$ for A. tamarense, and ${\mu}_{max}$ of 0.38 $day^{-1}$ with $K_I$ of 59.53 ${\mu}molm^{-2}s^{-1}$, and $I_c$ was 40.80 ${\mu}molm^{-2}s^{-1}$ for A. catenella. The $I_c$ equated to a depth of 8~9 m from March to June for A. tamarense and 6~7 m from March to June for A. catenella. These responses suggested that irradiance at the depth near the middle layer in Masan Bay would provide favorable conditions for two species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.168-172
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• 1996

The soil moisture content and its relation to plants may be important in determining the crop growth and yield. The present study was undertaken to evaluate the leaf water potential and photosynthetic activity in soybean plants as affected by the timing of soil water stress. The soybean variety, 'Tachinagaha', was grown in a pot. The 15 day-old seedlings were subject to the three levels of soil moisture content(25, 40 and 55%) for 25 days. Then the treated soybean plants were placed again at the level of 25% soil moisture content for 25 days, and were compared with the control which was well-watered at 40% level for whole growth period. Soybean plants grown under continuous drought showed higher apparent photosynthetic rate(AP) than those under well-watering /drought in the first /second water treatment, suggesting that AP was adjusted after previous acclimation to drought. Over a wide range of photosynthetic photon flux densities(PPFD), drought or excessive water stress resulted in the decrease in AP when compared with the control. AP and stomatal conductance were decreased in soybean plants subject to water deficit stress, suggesting that AP and stomatal conductance were more sensitive to drought than excessive water stress.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.227-234
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• 2014

The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and 'Chipper'), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to $4^{\circ}C$ (Experiment 1) and $1^{\circ}C$ (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and $149{\mu}moles{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and 'Chipper' after chilling w as 1 and 1.1, respectively. Korean 'Nacdongchungjang' was most sensitive to chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to chilling of 'Nacdongchungjang' was followed by Chinese 'Dongguan' [DR = 1.7]. In contrast, 'Saeronchungjang' (DR = 1) and 'Janghyungnachap' (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and 'Chipper'. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and 'Chipper') and all Korean lines examined.

• Journal of Microbiology and Biotechnology
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• v.21 no.12
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• pp.1306-1311
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• 2011

Recombinant Rhodopseudomonas palustris, harboring the carotenoid-metabolizing gene crtI (CrtIBS), and whose color changes from greenish yellow to red in response to inorganic As(III), was cultured in transparent microplate wells illuminated with a light emitting diode (LED) array. The cells were seen to grow better under near-infrared light, when compared with cells illuminated with blue or green LEDs. The absorbance ratio of 525 to 425 nm after cultivation for 24 h, which reflects red carotenoid accumulation, increased with an increase in As(III) concentrations. The detection limit of cultures illuminated with near-infrared LED was 5 ${\mu}g$/l, which was equivalent to that of cultures in test tubes illuminated with an incandescent lamp. A near-infrared LED array, in combination with a microplate, enabled the simultaneous handling of multiple cultures, including CrtIBS and a control strain, for normalization by the illumination of those with equal photon flux densities. Thus, the introduction of a near-infrared LED array to the assay is advantageous for the monitoring of arsenic in natural water samples that may contain a number of unknown factors and, therefore, need normalization of the reporter event.

• Journal of The Korean Society of Agricultural Engineers
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• v.52 no.5
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• pp.11-18
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• 2010

This study was conducted to provide design data for deciding covering method in double layers greenhouse. The variation of photosynthetic photon flux (PPF) and heat flow in air inflated and conventional double layers greenhouse was analyzed. The PPF of air inflated double covering greenhouse was less than that of conventional greenhouse during summer season because the more PPF comes into conventional greenhouse through roof vent which was rolled up for ventilation. The air inflated double layers covering greenhouse was superior to conventional type in the aspect of controlling inside temperature down owing to lower irradiation. The PPF of air inflated greenhouse was greater than that of conventional greenhouse during winter season because the transmittance of conventional greenhouse decreased by dust collected on inside plastic film nearly closed for insulation. Considering the PPF not sufficient for tomato growing in winter, the air inflated double covering system with the greater transmittance was better than conventional covering system. When the inside air of air inflated greenhouse was injected into space between the double layers of covering, the PPF of air inflated greenhouse was much less than the conventional greenhouse because the transmittance of air inflated double covering decreased due to condensation of highly humidified inside air. It was concluded that the more dried outside air should be used for inflating double layers covering. The heat insulation performance of air inflated double covering system was superior to conventional double covering system when comparing the overall heat transfer coefficients for each covering method. However the differences among the overall heat transfer coefficients depending on difference between inside and outside temperatures of greenhouse were great, it is necessary to conduct additional experiment for investigating the overall heat transfer coefficient to design the double layers covering.

• Journal of Bio-Environment Control
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• v.11 no.2
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• pp.88-92
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• 2002

The study was conducted to estimate stimulation of plant growth of blue and red leaf lettuce (Lactuca sativa L.) as affected by covering methods with vinyl materials containing concentrated PO film. Plants were grown in direct, direct and tunnel, and tunnel covering with 'Pasraido materials' from October 13 to 31. With the covering mean air temperature, soil temperature, leaf temperature and relative humidity remarkedly increased. Higher temperature was observed in the order of direct and tunnel, direct, tunnel covering and the control. Photosynthetic photon flux (PPF, $\mu$mol m$^{-2}$ . s$^{-1}$ ) was observed in reverse order of temperature. Concentration of $CO_2$ was higher during night than during day under the light. Accumulation of highest $CO_2$ at night was obtained from direct coverings followed by direct and tunnel, tunnel covering and the control. Plant height, leaf number and leaf area of blue and red lettuces were stimulated by direct covering. However, there was no significant difference.