• 한국정밀공학회지
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• 제12권6호
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• pp.87-95
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• 1995

In order to predict the case depth and case width in laser transformation hardening, a finite element method was used to analyze the temperature distribution on the material. Laser hardening of the specimens of SM45C and STE11steels was experimented by using the continuous wave CO$_{2}$ laser with the various travel speeds and the defocused Gaussian beam mode. Phosphate coating was adopted on the surface of SM45C to increase the absorption of 10.6 .mu. m laser energy. Experimental data show good agreement with the theoretical predictions. The maximum possible case depth can be predicted for the given laser hardening conditions, such as laser power, and travel speed.

• 한국자기학회지
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• 제15권5호
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• pp.270-275
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• 2005

가스분무법으로 제조한 $35\~180\;{\mu}m$ 크기의 $Fe-6.0wt\%Si$ 합금분말에 대하여 인산염계 절연물질을 피막처리하고 $600\~900^{\circ}C$에서 1시간 동안 열처리한 후 압축성형한 압분자심(분말코아)의 자기적 성질 및 기계적 특성을 조사하였다. 대체적으로 열처리온도가 증가할수록 압축강도가 감소하였으며, 자심손실 또한 낮아졌다. $800^{\circ}C$에서 열처리한 경우 압축성형강도가 15 kgf, 100 kHz에서 실효투자율은 74, 품질계수는 26, 50 Oe의 직류자장 하에서 퍼센트투자율은 78 정도의 값을 나타내었으며, 50 kHz-0.1 T에서 자심손실은 $750\;mW/cm^3$였다. 그리고 투자율-주파수 곡선 상에서의 cut-off 주파수는 거의 200 kHz 이상에 이르는 것으로 나타났다. $Fe-6.0wt\%Si$ 합금 압분자심의 이러한 제반 특성은 인산염 피막의 양호한 전기절연효과와, 고규소농도에 따른 합금의 결정자기이방성 및 포화자기변형의 감소, 전기비저항의 증대 등 기초 물성의 변화에 기인하는 것으로 사료되었다.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2009년도 추계학술발표대회
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• pp.41.1-41.1
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• 2009

Aerosol Deposition (AD) is anovel way to fabricate bioactive ceramic coatings in biomedical implants and prostheses applications. In the present work, silicon-substituted hydroxyapatite (HA) coatings on commercially pure titanium were prepared by aerosol deposition using Si-HA powders. The incorporation of silicon in the HA lattice is known to improve the bioactivity of the HA, makingsilicon-substitute HA an attractive alternative to pure HA in biomedical applications. Si-HA powders with the chemical formula $Ca_{10}(PO_4)_6-x(SiO_4)x(OH)_2-x$, having silicon contents up to x=0.5 (1.4 wt%), were synthesized by solid-state reaction of $Ca_2P_2O_7$, $CaCO_3$, and $SiO_2$. The Si-HA powders were characterized by X-ray diffraction (XRD), X-ray fluorescence spectrometry (XRF), and Fourier transform infrared spectroscopy(FT-IR). The corresponding coatings were also analyzed by XRD, scanning electron microscopy (SEM), and electron probe microanalyzer (EPMA). The results revealed that a single-phase Si-HA was obtained without any secondary phases such as $\alpha$- or $\beta$-tricalcium phosphate (TCP) for both the powders and the coatings.The Si-HA coating was about $5\;{\mu}m$ thick, had a densemicrostructure with no cracks or pores. In addition, the proliferation and alkaline phosphatase (ALP) activity of MC3T3-E1 preosteoblast cells grown on the Si-HA coatings were significantly higher than those on the bare Ti and pure HA coating. These results revealed the stimulatory effects induced by siliconsubstitution on the cellular response to the HA coating.

• 한국식품과학회지
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• 제31권3호
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• pp.771-777
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• 1999

CSLM 기술을 이용하여 Salmonella enteritidis에 의한 계란 오염 과정을 관찰하여 난막의 3중막 구조, cuticle 층 그리고 5가지 필름 피복의 침투 방어효과를 조사하였다. 난막을 구성하는 fiber의 지름은 각각 outer membrane $1.5{\sim}7.2\;{\mu}m$, inner membrane $0.8{\sim}2.0\;{\mu}m$이었고, limiting membrane을 구성하는 입자의 지름은 $0.1{\sim}1.4\;{\mu}m$이었으며, 두께는 각각 평균 10.0, 3.5, $3.6\;{\mu}m$이었다. 난각과 접해 있는 outer membrane은 다음 층인 inner membrane과 부분적으로 중첩되어있고, limiting membrane의 입자들은 매우 밀집되어 inner membrane과 중첩된 상태로 존재하였다. Cuticle층은 전체 계란 표면적 중 $40{\sim}80%$ 를 덮고 있었으며, 두께는 평균 $6.0\;{\mu}m$이었고 피복 필름들의 평균 두께는 자각 mineral oil $5.0\;{\mu}m$, dextrin $3.6\;{\mu}m$, gelatin $3.9\;{\mu}m$, starch $2.5\;{\mu}m$, chitosan $2.2\;{\mu}m$이었다. 필름 피복에 의한 Salmonella enteritidis 생육 억제효과를 관찰한 결과, chitosan이 가장 효과적이었다. 그리고 trisodium phosphate (TSP), cetylpyridinium chloride (CPC) 처리, 5종의 필름 피복 처리와 무처리한 계란표면에 Salmonella enteritidis를 접종한 후 내부 전이 과정을 CSLM을 이용하여 다색영상화(multi-color imaging)와 시간당 투과 균수에 대한 plate count로 비교한 결과, 난각이나 3중막 구조의 난막보다는 cuticle 층이 Salmonella균의 오염을 차단하는데 결정적인 역할을 하는 것으로 나타났으며, chitosan 피복이 cuticle 층과 비슷한 효과를 보였다. 따라서 피복 필름중에서 chitosan이 최외부 방어막인 cuticle층이 결손된 난각 부위를 피복하는데 가장 적합할 것으로 사료된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제23권5호
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• pp.396-405
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• 2001

Osseointegrated implants are used for the fixation of dental prosthesis with good long-term clinical results. In an attempt to improve the quantity and quality of the bone-implant interface, numerous implant modification have been used. Implants surface modifications have been used such as titanium-plasma sprayed, hydroxyapatite-coating, sandblasted, sandblasted and acid-etched, acid-etched. Rough surface implants have greater implant surface area and enhance the bone-implant interface and improve stabilization. The purpose of present study was to evaluate light microscopic and scanning microscopic examinations and removal torque value of newly developed calcium phosphate blast and acid-etched implant in the femur of rabbits. Titanium plasma sprayed(TPS) implant served as controls. After 12 weeks of healing of the femurs of 12 rabitts, the implant-containing segments of femur were removed on bloc and bone block including sections. Histologic examination and histomorphometric and removal torque values comparision were made for two implants. Obtained results are follows: 1. Newly developed calcium phosphate blasted and acid-etched implants were in close contact with bone under light microscopic examinations. 2. New implants showed mean bone-to implant contact 59.8%, whereas TPS implants showed mean bone-to implant contact 54.5% (statistically no difference p<0.05). 3. New implants showed mean bone density 56.7%, whereas TPS implants showed mean bone density 49.2% (statistically difference p<0.05). 4. New implants demonstrated mean removal torque values 40.5Ncm, whereas the mean removal torque values of TPS implants ranged 39.3Ncm. No statistical differences(p<0.05) were observed between two groups of implants nor was there any difference between the two implants at the clinical level.

• The Journal of Advanced Prosthodontics
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• 제2권4호
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• pp.142-147
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• 2010

PURPOSE. One of the major keys to achieve successful osseointegration of the implant is its surface properties. The aim of this study was to investigate the bone response to dental implants with different surface characteristics using the rabbit tibia model. Tricalcium phosphate (TCP) coated, anodic oxidized and turned (control) surfaces were compared. MATERIALS AND METHODS. Seventy two implants were placed in the tibia of eighteen rabbits. Nine rabbits were sacrificed at 3 weeks of healing and the remaining nine were sacrificed at 6 weeks of healing. The bone-to-implant contact (BIC) and the bone volume density (BVD) were assessed by light microscope after 3 and 6 weeks of healing. RESULTS. Statistical analysis showed that no significant differences in the BIC and BVD were observed between the different implant surfaces and the control group at 3 weeks and 6 weeks of healing. Data also suggested that the BVD of all the surfaces showed significant difference at 3 and 6 weeks. CONCLUSION. The present study has showed that osseointegration occurred in all investigated types of surface-treated implants. In the current study all of the threads of the implants were observed to calculate BIC and BVD values (instead of choosing some of the threads from the bone cortex for example), which didn't make BIC or BVD percentage values better than in the control group, therefore the clinical relevance of these results remains to be shown.

• Bulletin of the Korean Chemical Society
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• 제35권12호
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• pp.3553-3558
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• 2014

The reduced graphene oxide(rGO)/aluminum phosphate($AlPO_4$)-coated $LiMn_{1.5}Ni_{0.5}O_4$ (LMNO) cathode material has been developed by hydroxide precursor method for LMNO and by a facile solution based process for the coating with GO/$AlPO_4$ on the surface of LMNO, followed by annealing process. The amount of $AlPO_4$ has been varied from 0.5 wt % to 1.0 wt %, while the amount of rGO is maintained at 1.0 wt %. The samples have been characterized by X-ray diffraction, scanning electron microscopy, and high-resolution transmission electron microscopy. The rGO/$AlPO_4$-coated LMNO electrodes exhibit better cyclic performance compared to that of pristine LMNO electrode. Specifically, rGO(1%)/$AlPO_4$(0.5%)- and rGO(1%)/$AlPO_4$(1%)-coated electrodes deliver a discharge capacity of, respectively, $123mAhg^{-1}$ and $122mAhg^{-1}$ at C/6 rate, with a capacity retention of, respectively, 96% and 98% at 100 cycles. Furthermore, the surface-modified LMNO electrodes demonstrate higher-rate capability. The rGO(1%)/$AlPO_4$(0.5%)-coated LMNO electrode shows the highest rate performance demonstrating a capacity retention of 91% at 10 C rate. The enhanced electrochemical performance can be attributed to (1) the suppression of the direct contact of electrode surface with the electrolyte, resulting in side reactions with the electrolyte due to the high cut-off voltage, and (2) smaller surface resistance and charge transfer resistance, which is confirmed by total polarization resistance and electrochemical impedance spectroscopy.

• Journal of Periodontal and Implant Science
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• 제42권5호
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• pp.166-172
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• 2012

Purpose: The aim of this study was to evaluate the improvement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with synthetic cell-binding peptide sequences in a standardized rabbit sinus model. Methods: Standardized 6-mm diameter defects were created bilaterally on the maxillary sinus of ten male New Zealand white rabbits, receiving BCP bone substitute coated with synthetic cell binding peptide sequences on one side (experimental group) and BCP bone substitute without coating (control group) on the other side. Histologic and histomorphometric analysis of bone formation was carried out after a healing period of 4 or 8 weeks. Results: Histological analysis revealed signs of new bone formation in both experimental groups (4- and 8-week healing groups) with a statistically significant increase in bone formation in the 4-week healing group compared to the control group. However, no statistically significant difference in bone formation was found between the 8-week healing group and the control group. Conclusions: This study found that BCP bone substitute coated with synthetic cell-binding peptide sequences enhanced osteoinductive potential in a standardized rabbit sinus model and its effectiveness was greater in the 4-week healing group than in the 8-week healing group.

• The Plant Pathology Journal
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• 제37권6호
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• pp.555-565
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• 2021

Bacteriophages infecting Acidovorax citrulli, the causal agent of bacterial fruit blotch, have been proven to be effective for the prevention and control of this disease. However, the occurrence of bacteriophage-resistant bacteria is one of hurdles in phage biocontrol and the understanding of phage resistance in this bacterium is an essential step. In this study, we aim to investigate possible phage resistance of A. citrulli and relationship between phage resistance and pathogenicity, and to isolate and characterize the genes involved in these phenomena. A phage-resistant and less-virulent mutant named as AC-17-G1 was isolated among 3,264 A. citrulli Tn5 mutants through serial spot assays and plaque assays followed by pathogenicity test using seed coating method. The mutant has the integrated Tn5 in the middle of a cupin protein gene. This mutant recovered its pathogenicity and phage sensitivity by complementation with corresponding wild-type gene. Site-directed mutation of this gene from wild-type by CRISPR/Cas9 system resulted in the loss of pathogenicity and acquisition of phage resistance. The growth of AC-17-G1 in King's B medium was much less than the wild-type, but the growth turned into normal in the medium supplemented with D-mannose 6-phosphate or D-fructose 6-phosphate indicating the cupin protein functions as a phosphomannos isomerase. Sodium dodecyl sulfa analysis of lipopolysaccharide (LPS) extracted from the mutant was smaller than that from wild-type. All these data suggest that the cupin protein is a phosphomannos isomerase involved in LPS synthesis, and LPS is an important determinant of pathogenicity and phage susceptibility of A. citrulli.

• 한국세라믹학회지
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• 제49권3호
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• pp.247-252
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• 2012

Crystal structure and chemical compositions of Plasma electrolytic oxidized layer of A-1100, A-2024, A-5052, A-6061, A-6063, A-7075, ACD-7B and ACD-12 were investigated. The electrolyte for plasma electrolytic oxidation was mixture of distilled water, $Na_2P_2O_7$, Cu, Cr metal salts and KOH. ${\eta}$-Alumina, as well as ${\alpha}$-alumina, was main crystal phase. Another crystals such as $(Al_{0.948}Cr_{0.052})_2O_3$ and $(Al_{0.9}Cr_{0.1})_2O_3$ were also formed in the oxide layer. It was thought that the effect of electrolyte compositions on the physical properties and crystal system of PEO layers was greater than the effect of Al alloy composition variation.