• Title/Summary/Keyword: Phenylpropanoid

Search Result 118, Processing Time 0.029 seconds

Isolation of a New Phenylpropanoid from Codonopsis ussuriensis

  • Lee, Ihn-Rhan;Seo, Eun-Kyong
    • Archives of Pharmacal Research
    • /
    • v.13 no.4
    • /
    • pp.365-366
    • /
    • 1990
  • A new phenylpropanoid was isolated from the roots of Codonopsis ussuriensis (Rupr. et Maxim) Hemsley. It was coloress crystals, mp. 140-142.deg.C and was elucidated as 4-(3-ethoxy-1-propenyl)-2, 6-dimethoxyphenyl-$\beta$-D-glucopyranoside on basis of spectral data analysis.

  • PDF

Fungal and Plant Phenylalanine Ammonia-lyase

  • Hyun, Min-Woo;Yun, Yeo-Hong;Kim, Jun-Young;Kim, Seong-Hwan
    • Mycobiology
    • /
    • v.39 no.4
    • /
    • pp.257-265
    • /
    • 2011
  • L-Phenylalanine is one of the essential amino acids that cannot be synthesized in mammals in adequate amounts to meet the requirements for protein synthesis. Fungi and plants are able to synthesize phenylalanine via the shikimic acid pathway. L-Phenylalanine, derived from the shikimic acid pathway, is used directly for protein synthesis in plants or metabolized through the phenylpropanoid pathway. This phenylpropanoid metabolism leads to the biosynthesis of a wide array of phenylpropanoid secondary products. The first step in this metabolic sequence involves the action of phenylalanine ammonialyase (PAL). The discovery of PAL enzyme in fungi and the detection of $^{14}CO_2$ production from $^{14}C$-ring-labeled phenylalanine and cinnamic acid demonstrated that certain fungi can degrade phenylalanine by a pathway involving an initial deamination to cinnamic acid, as happens in plants. In this review, we provide background information on PAL and a recent update on the presence of PAL genes in fungi.

Detection of Wound-inducible Trans-Cinnamic Acid-4-Hydroxylase in Avocado, Persea americana, Roots

  • Joo, Eun-Young
    • Preventive Nutrition and Food Science
    • /
    • v.2 no.4
    • /
    • pp.333-337
    • /
    • 1997
  • Trans-cinnamic acid-4-hydroxylase(tC4H) is the first cytochrome P450-dependent monooxygenase of the phenylpropanoid pathway. The roots of avocado seedlings were wounded and examined to determine whether the tC4H would be activated in response to wounding and/or whether tC4H activity be modulated by the application of exogenous p-coumarate. At the specified length of times, the wounded and treated roots were either frozen in liquid nitrogen or used immediately to extract microsomal proteins. The microsomal proteins were subjected to immunoblot analysis using polyclonal antibodies against CYP73 of tC4H gene. In this study, tC4H was induced in wounded roots sealed in bags within 6 hours, and in low level({TEX}$10^{-8}${/TEX}M) of p-coumarate solution within 24 hours, whereas the olution without p-coumarate and high levels of p-coumarate solution repressed tC4H induction in wounded roots. These results indicate that tC4H is induced by wounding in the root of avocado, and is inhibited by the application of exogenous p-coumarate.

  • PDF

Glycosides from the Flower of Clerodendrum trichotomum (누리장나무 꽃의 배당체 성분)

  • Lee, Jong-Wook;Bae, Jong Jin;Kwak, Jong Hwan
    • Korean Journal of Pharmacognosy
    • /
    • v.47 no.4
    • /
    • pp.301-306
    • /
    • 2016
  • Four phenylpropanoid glycosides and a monoterpene glycoside were isolated from the flower of Clerodendrum trichotomum. Structures of the isolated compounds were identified as acteoside (1), martynoside (2), leucosceptoside A (3), isoacteoside (4) and neohancoside A (5) by spectroscopic analysis. Compounds 1-4 were isolated from the flower of C. trichotomum for the first time. Compound 5 was first obtained from genus Clerodendrum as well as family Verbenaceae. The antioxidant activity of compounds 1-5 were evaluated by the DPPH free radical scavenging assay. Compounds 1-4 exhibited strong antioxidant activity.

Phenolic Compounds from the Inner Bark of Paulownia coreana

  • Si, Chuan-Ling;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
    • /
    • v.35 no.5
    • /
    • pp.93-99
    • /
    • 2007
  • Paulownia coreana inner bark was collected, extracted in 70% acetone, concentrated under reduced pressure and sequentially fractionated using n-hexane, $CH_2Cl_2$, EtOAc and $H_2O$, then freeze dried to give brown powders. A portion of the EtOAc soluble powder was chromatographed on a Sephadex LH-20 column using a serious of aqueous methanol and ethanol-hexane mixture as eluting solvents. Two phenolic acid, $\rho$-courmaric acid and caffeic acid, two isomeric phenylethanoid glycosides, verbascoside and iso-verbascoside, and one epimeric phenylpropanoid glycoside, cistanoside F, were isolated and their structures were elucidated by spectroscopic analysis such as NMR and MS.

Activities of Sulfhydryl-Related and Phenylpropanoid-Synthesizing Enzymes during Leaf Development of Arabidopsis thaliana

  • Sa, Jae-Hoon;Park, Eun-Hee;Lim, Chang-Jin
    • BMB Reports
    • /
    • v.31 no.6
    • /
    • pp.554-559
    • /
    • 1998
  • Activities of glutathione- and thioredoxin-related enzymes and phenylpropanoid-synthesizing enzymes were measured and compared in the developing leaves of Arabidopsis thaliana. Phenylalanine ammonia-lyase activity is maximal in the leaves of 2-wk-grown Arabidopsis. Tyrosine ammonia-lyase activity is maximal in the leaves of 3-wk-grown and 4-wk-grown Arabidopsis. Activity of thioitransferase, an enzyme involved in the reduction of various disulfide compounds, is higher in younger leaves than in older ones. A similar pattern was obtained in the activity of thioredoxin, a small protein known as a cofactor of ribonucleotide reductase and a regulator of photosynthesis. Activity of glutathione reductase is also higher in the younger leaves. Malate debydrogenase activity remains relatively constant during the development of Arabidopsis leaves. The results offer preliminary information for further approach to elucidate the mechanism of growth-dependent variations of these enzymes.

  • PDF

11-Methoxyviburtinal, A New Iridoid from Valeriana jatamansi

  • Chen Ye-Gao;Yu Li-Li;Huang Rong;Lv Yu-Ping;Gui Shi-Hong
    • Archives of Pharmacal Research
    • /
    • v.28 no.10
    • /
    • pp.1161-1163
    • /
    • 2005
  • Five compounds of iridoids, lignan and phenylpropanoid glycosides were isolated from the roots of Valeriana jatamansi by column chromatography. Their structures were elucidated as 11-methoxyviburtinal (1), baldrinal (2), prinsepiol-4-O-${\beta}$-D-glucoside (3), coniferin (4), and hexacosanic acid (5) by spectroscopic analysis. 11-Methoxyviburtinal was a new compound, and others were isolated from the plant for the first time.

Two New Phenylpropanoid Glycosides from the Stem Bark of Acanthopanax trifoliatus

  • Kiem, Phan-Van;Minh, Chau-Van;Dat, Nguyen-Tien;Cai, Xing-Fu;Lee, Jung-Joon;Kim, Young-Ho
    • Archives of Pharmacal Research
    • /
    • v.26 no.12
    • /
    • pp.1014-1017
    • /
    • 2003
  • Two new phenylpropanoid glycosides, 1-$\beta$-D-glucopyranosyl-2,6-dimethoxy-4-propenylphenol (1) and 1-[$\beta$-D-glucopyranosyl-(1$\rightarrow6)-\beta$-D-glucopyranosyl]-2,6-dimethoxy-4-propenylphenol (2) were isolated from the stem bark of Acanthopanax trifoliatus along with four known compounds (3∼6). Their structures were established on the basis of spectral and chemical evidences.

HIV-1 Integrate Inhibitory Phenylpropanoid Glycosides from Clerodendron trichotomum

  • Kim, Hyoung-Ja;Woo, Eun-Rhan;Shin, Cha-Gyun;Hwang, Dong-Jin;Park, Ho-koon;Lee, Yong-Sup
    • Archives of Pharmacal Research
    • /
    • v.24 no.4
    • /
    • pp.286-291
    • /
    • 2001
  • Seven phenylpropanoid glycosides named acteoside (1), acteoside isomer (2), leucosceptoside A (3), plantainoside C (4), jionoside D (5), martynoside (6), and isomartynoside (7) were isolated from Clerodendron trichotomum. Compounds 1 and 2 showed potent inhibitory activities against HlV-1 integrase with $IC_{20}$ values of 7.8 ${\pm}$ 3.6 and 13.7 ${\pm}$ 6.0${\mu}\textrm{M}$, respectively.

  • PDF

Isolation of Hepatoprotective Phenylpropanoid from Lactuca indica

  • Kim, Ki-Hyun;Kim, Young-Ho;Lee, Kang-Ro
    • Natural Product Sciences
    • /
    • v.16 no.1
    • /
    • pp.6-9
    • /
    • 2010
  • In continuing our search for biologically active compounds from Korean Compositae medicinal plants, we investigated the constituents of the aerial parts of Lactuca indica L. and isolated a phenylpropanoid derivative from its MeOH extract. The chemical structure was characterized by spectroscopic methods, including 1D and 2D NMR to be di-E-caffeoyl-meso-tartaric acid (1). Compound 1 was isolated for the first time from this plant. In this paper, we suggest that the NMR assignment at C-2 of (+)-taraxafolin-B should be corrected. In the human HBV-transfected liver cell line HepG2.2.15, the compound 1 effectively reduced HBV DNA level in the release of mature HBV particles from HepG2.2.15 cultivation.