• Title/Summary/Keyword: Phenotypic Traits

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Genetic Studies and Development of Prediction Equations in Jersey${\times}$Sahiwal and Holstein-Friesian${\times}$Sahiwal Half Breds

  • Singh, P.K.;Kumar, Dhirendra;Varma, S.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.2
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    • pp.179-184
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    • 2005
  • First lactation records (174) of Jersey${\times}$Sahiwal and Holstein Friesian${\times}$Sahiwal half breds under 9 sires maintained at Chandra Shekher Azad University of Agriculture and Technology, Kanpur, Uttar Pradesh, India from 1975-1983, were used to estimate the genetic parameters and to predict herd life milk yield and average milk yield per day of herd life from first lactation traits. The traits included were: age at first calving, first service period, first lactation period, first calving interval, first lactation milk yield, milk yield per day of first calving interval, herd life milk yield, herd life and average milk yield per day of herd life. Most of the production and reproduction traits were found to have positive and significant correlations between them on genetic as well as phenotypic scales. Total twelve regression equations were fitted. The prediction equation of herd life milk yield in both the genetic groups showed linear relationship with AFC, FSP, FLP, FLMY and MY/DCI and was apparent and significant. Similarly, polynomials for milk yield per day of herd life for J${\times}$S and HF${\times}$S half breds also showed linear trend, which was found highly significant. The highest and lowest $R^2$ values were found for FCI and AFC, respectively.

Genetic and Environmental Effects on Carcass Traits of Japanese Brown Cattle

  • Sri Rachma Aprilita Bugiwati, T.D.;Harada, H.;Fukuh, R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.1
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    • pp.1-5
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    • 2000
  • Studies on the genetic and environmental effects on M.longissimus thoracis area (MLTA), fat thickness (SFT), rib thickness (RT) and marbling score (MS) were conducted on 21,086 steers and 7,151 heifers of Japanese Brown breed. All carcass traits were affected significantly (p<0.01) by sire, sex and initial year effects. Both of the MLTA and MS of steers were greater than heifers. Their differences were $1.4cm^2$ for MLTA and 0.05 for MS, respectively. Cattle started for fattening in winter tend to have higher of MLTA and MS and thicker of SFT and RT than those in other seasons. MLTA increase from 1987 to 1989 (about $1.9cm^2$) and decrease until 1994 (about $2.4cm^2$) and then increase again up to 1995 (about $1.5cm^2$). MS were nearly equal from 1987 to 1991 (about "1") and then decrease up to 1995 (about "1"). Heritability estimates of MLTA, RT, SFT and MS were ranged from 0.22 to 0.36. Genetic and phenotypic correlations of MLTA, RT, SFT and MS were positive and ranged from 0.05 to 0.62 and from 0.03 to 0.32 except SFT with MLTA was negative (-0.14 and -0.03).

Estimation of Interaction Effects among Nucleotide Sequence Variants in Animal Genomes

  • Lee, Chaeyoung;Kim, Younyoung
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.1
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    • pp.124-130
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    • 2009
  • Estimating genetic interaction effects in animal genomics would be one of the most challenging studies because the phenotypic variation for economically important traits might be largely explained by interaction effects among multiple nucleotide sequence variants under various environmental exposures. Genetic improvement of economic animals would be expected by understanding multi-locus genetic interaction effects associated with economic traits. Most analyses in animal breeding and genetics, however, have excluded the possibility of genetic interaction effects in their analytical models. This review discusses a historical estimation of the genetic interaction and difficulties in analyzing the interaction effects. Furthermore, two recently developed methods for assessing genetic interactions are introduced to animal genomics. One is the restricted partition method, as a nonparametric grouping-based approach, that iteratively utilizes grouping of genotypes with the smallest difference into a new group, and the other is the Bayesian method that draws inferences about the genetic interaction effects based on their marginal posterior distributions and attains the marginalization of the joint posterior distribution through Gibbs sampling as a Markov chain Monte Carlo. Further developing appropriate and efficient methods for assessing genetic interactions would be urgent to achieve accurate understanding of genetic architecture for complex traits of economic animals.

Identification of Superior Polyvoltine Hybrids (polyvoltine${\times}$bivoltine) of Silkworm, Bombyx mori L.

  • Rao, C.G.P.;Chandrashekharaiah;Basha, K.Ibrahim;Seshagiri, S.V.;Ramesh, C.;Nagaraju, H.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.1
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    • pp.43-49
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    • 2004
  • Ten promising polyvoltine mulberry silkworm strains(SDMGl, SDMG2, SDMG3, SDMG4, SDMWl, SDMW2, RMWl, RMW2, RMW3 and RMW4) that are superior in quantitative and qualitative traits have been synthesized in the polyvoltine breeding laboratory of Andhra Pradesh State Sericulture Research & Development Institute, Hindupur through systematic hybridization and appropriate selection methods. After the genotypes were found homozygous for the desired traits, they have been crossed with 3 bivoltine testers $(APS8, APS4 and {NB_2}{D_4})$ and thirty new hybrid combinations were developed for the assessment of their hybrid performance. Phenotypic expressions of economically important quantitative and qualitative traits of fist filial generation were measured and subjected for statistical analysis. Evaluation Index and Subordinate Function methods were employed for the assessment of hybrid performance since they are widely used in silkworm hybrid evaluation. Total of seven poly${\times}$bivoltine combinations, which ranked high in both the methods, were selected as potential combinations for further field test. These combinations also ranked significantly higher than the control hybrid (APMl${\times}$APS8).

Genome scan linkage analysis identifies a major quantitative trait loci for fatty acid composition in longissimus dorsi muscle in an F2 intercross between Landrace and Korean native pigs

  • Park, Hee-Bok;Han, Sang-Hyun;Yoo, Chae-Kyoung;Lee, Jae-Bong;Kim, Ji-Hyang;Baek, Kwang-Soo;Son, Jun-Kyu;Shin, Sang-Min;Lim, Hyun-Tae;Cho, In-Cheol
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.8
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    • pp.1061-1065
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    • 2017
  • Objective: This study was conducted to locate quantitative trait loci (QTL) influencing fatty acid (FA) composition in a large $F_2$ intercross between Landrace and Korean native pigs. Methods: Eighteen FA composition traits were measured in more than 960 $F_2$ progeny. All experimental animals were genotyped with 165 microsatellite markers located throughout the pig autosomes. Results: We detected 112 QTLs for the FA composition; Forty seven QTLs reached the genome-wide significant threshold. In particular, we identified a cluster of highly significant QTLs for FA composition on SSC12. QTL for polyunsaturated fatty acid on pig chromosome 12 (F-value = 97.2 under additive and dominance model, nominal p-value $3.6{\times}10^{-39}$) accounted for 16.9% of phenotypic variance. In addition, four more QTLs for C18:1, C18:2, C20:4, and monounsaturated fatty acids on the similar position explained more than 10% of phenotypic variance. Conclusion: Our findings of a major QTL for FA composition presented here could provide helpful information to locate causative variants to improve meat quality traits in pigs.

Monitoring conservation effects on a Chinese indigenous chicken breed using major histocompatibility complex B-G gene and DNA Barcodes

  • Tu, Yunjie;Shu, Jingting;Ji, Gaige;Zhang, Ming;Zou, Jianmin
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.10
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    • pp.1558-1564
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    • 2018
  • Objective: We report monitoring conservation effect for a Chinese indigenous chicken (Langshan) breed using major histocompatibility complex (MHC) and DNA barcords. Methods: The full length of MHC B-G gene and mitochondrial cytochrome oxidase I (COI) gene in generations 0, 5, 10, 15, 16, and 17 was measured using re-sequencing and sequencing procedures, respectively. Results: There were 292 single nucleotide polymorphisms of MHC B-G gene identified in six generations. Heterozygosity (He) and polymorphic information content (PIC) of MHC B-G gene in generations 10, 15, 16, and 17 remained stable. He and PIC of MHC B-G gene were different in six generations, with G10, G15, G16, G17 >G5>G0 (p<0.05). For the COI gene, there were five haplotypes in generations 0, 5, 10, 15, 16, and 17. Where Hap2 and Hap4 were the shared haplotypes, 164 individuals shared Hap2 haplotypes, while Hap1 and Hap3 were the shared haplotypes in generations 0 and 5 and Hap5 was a shared haplotype in generations 10, 15, 16, and 17. The sequence of COI gene in 6 generations was tested by Tajima's and D value, and the results were not significant, which were consistent with neutral mutation. There were no differences in generations 10, 15, 16, and 17for measured phenotypic traits. In other generations, for annual egg production, with G5, G10, G15, G16, G17>G0 (p<0.05). For age at the first egg and age at sexual maturity, with G10, G15, G16, G17>G5>G0 (p<0.05). Conclusion: Combined with the results of COI gene DNA barcodes, MHC B-G gene, and phenotypic traits we can see that genetic diversity remained stable from generations 10 to 17 and the equimultiple random matching pedigrees conservation population conservation effect of Langshan chicken was effective as measured by these criteria.

Genetic Diversity of Phenotypic Traits and Biochemicals of Lettuce (Lactuca sativa L.) Germplasm

  • On-Sook Hur;Ho-Cheol Ko;Na-Young Ro;Awaris Derbie Assefa;Aejin Hwang;Bichsaem Kim;Seong-Hoon Kim;Youn Jeong Lee;Hee-Jong Woo;Jung-Yoon Yi;Bum-Soo Hahn
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.73-73
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    • 2022
  • Lettuce (Lactuca sativa L., Family Asteraceae) is highly ranked in production and economic value and is consumed either fresh or in salad mixes because of its important dietary source of bioactive phytochemicals. The world collection of Lactuca spp. leafy crops, maintained in NAC, includes 2,464 accessions from 71 countries around the world, of which 2,411 belong to L. sativa species, nineteen to L. saligna, and fifteen to L. serriolar. We aimed to investigate the lettuce germplasm with morphological and biochemical analyses and provide new material for breeding. The lettuce crop comprises seven main groups of cultivars (including oilseed lettuce) differing phenotypically. Agricultural characteristcs were investigated including time to bolting, time to flowering, seed color, flower color, leaf attitude, leaf color, leaf anthocyanin coloration, type of incision of margin, depth of incisions of margins, and leaf venation. Screening of the health beneficial metabolites like anthocyanin and bitter sesquiterpene lactones (lactucin and lactucopicrin) was also conducted. The range of anthocyanin and SLs were 0~563.78 mg/100g D.W. and 3.74~3311.66 ug/g D.W., respectively. The investigation of the degree of variation regarding phenotypic traits and biochemical revealed adaptive stable and highly variable use of trait collection.

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Development of targeted amplicon next-generation sequencing panel of 50 SNPs related to externally visible characteristics and behavior (외형 및 행동 습관 관련 50개 SNP 마커 분석을 위한 targeted amplicon next-generation sequencing 패널 개발)

  • Hee-Yeon Park;Yoonji Noh;Eung-Soo Kim;Hyun-Chul Park
    • Analytical Science and Technology
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    • v.37 no.3
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    • pp.189-199
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    • 2024
  • In forensic genetics, when it is not possible to confirm an individual's identity through STR profile analysis, additional information about the individual can be obtained using DNA-based phenotypic traits estimation. Recently, various researches have been conducted on methods to determine externally visible characteristics (EVC) such as eyes, hair, and skin color. However, relying solely on such phenotypic traits information has limitations for application in East Asian regions, including Korea. In this study, in order to utilize EVC related to an individual's appearance as investigative information, SNPs related to eye shape, hair thickness, skin color, as well as baldness, body type, high myopia, facial shape, acne, and behavioral habits were explored. A total of 50 SNPs were selected, and a targeted amplicon NGS panel capable of amplifying them all at once was developed. Experimental results confirmed the allelic types and frequencies of the 50 SNPs in 14 samples. We plan to use this panel to investigate the correlation between genotype and phenotype using various samples, and to develop methods for interpreting the results.

Phenotype Comparison between Herbicide Tolerant Transgenic Rice and Weedy Rice (제초제 내성 형질전환 벼와 잡초성 벼의 표현형질 비교)

  • Go, Eun Mi;An, Joo Hee;Nam, Ki Jung;Nam, Kyong-Hee;Park, Kee Woong;Back, Kyoungwhan;Kim, Chang-Gi
    • Weed & Turfgrass Science
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    • v.2 no.1
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    • pp.15-22
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    • 2013
  • The phenotypic traits of herbicide-tolerant transgenic rice were compared with those of wild type (Dongjin) as well as two accessions (Hwaseong-aengmi 1 and Gwangyang-aengmi 12) of weedy rice. This study was conducted to investigate whether unintentional alterations in phenotypic characteristics occurred in the transgenic rice and whether the altered traits were similar to those in the two weedy rices. All qualitative traits studied were similar in the transgenic or wild-type rice. On the other hand, awn presence, flag leaf attitude and grain color differed considerably between herbicide-tolerant transgenic rice and weedy rice. As for quantitative traits, plant height, the number of tillers per plant and shoot dry weight were significantly greater for weedy rice than transgenic or wild-type rice. Grain weight per plant and 1000-grain weight of transgenic (or wild-type) rice were significantly greater than those of weedy rice. Transgenic rice shattered less than the other rices. Amylose and protein contents in embryos of transgenic rice were significantly different from those of weedy rice. The potential for weediness of the transgenic rice may be assessed using phenotype comparison between transgenic and weedy rice as shown in this study.

Evaluations on agronomic traits of rice transgenic lines (벼 형질전환계통의 주요 작물학적 특성에 대한 고찰)

  • Jeong, Jong-Min;Jeung, Ji-Ung;Kang, Kyung-Ho;Lee, Sang-Bok;Park, Hyang-Mi;Kim, Chung-Kon;Kim, Kyung-Min;Sohn, Jae-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.2
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    • pp.196-202
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    • 2013
  • This study was conducted to evaluate the performances of rice transgenic lines along with their wild types in terms of agronomic traits. A total of 32 rice transgenic lines, through previously conducted collaborative researches between molecular biologists and conventional rice breeders, were selected as promising lines. As the introduced functional genes, 17 genes, which were putatively related with high yield, disease and herbicide resistance, abiotic stress tolerance, and diversifying endosperm starch components, were transformed into three Japonica cultivars, Nipponbare, Nagdongbyeo, and Dongjinbyeo. The transgenic lines exhibited significantly deviated performances from their wild types on agronomic traits such as days to heading, culm length and yield potential. Multivariate analyses on transgenic lines to the evaluated agronomic traits also indicated random manner of phenotypic deviations from their wild type in terms of deviation directions and degrees. Our results suggested that, therefore, breeding strategies to control unexpected deleterious phenotypic performances among transgenic lines would be critical as much as the functions and proper expressions of the transformed genes.