• Title/Summary/Keyword: Phenotypes

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Mouse Models of Gastric Carcinogenesis

  • Yu, Sungsook;Yang, Mijeong;Nam, Ki Taek
    • Journal of Gastric Cancer
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    • v.14 no.2
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    • pp.67-86
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    • 2014
  • Gastric cancer is one of the most common cancers in the world. Animal models have been used to elucidate the details of the molecular mechanisms of various cancers. However, most inbred strains of mice have resistance to gastric carcinogenesis. Helicobacter infection and carcinogen treatment have been used to establish mouse models that exhibit phenotypes similar to those of human gastric cancer. A large number of transgenic and knockout mouse models of gastric cancer have been developed using genetic engineering. A combination of carcinogens and gene manipulation has been applied to facilitate development of advanced gastric cancer; however, it is rare for mouse models of gastric cancer to show aggressive, metastatic phenotypes required for preclinical studies. Here, we review current mouse models of gastric carcinogenesis and provide our perspectives on future developments in this field.

WTO, an ontology for wheat traits and phenotypes in scientific publications

  • Nedellec, Claire;Ibanescu, Liliana;Bossy, Robert;Sourdille, Pierre
    • Genomics & Informatics
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    • v.18 no.2
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    • pp.14.1-14.11
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    • 2020
  • Phenotyping is a major issue for wheat agriculture to meet the challenges of adaptation of wheat varieties to climate change and chemical input reduction in crop. The need to improve the reuse of observations and experimental data has led to the creation of reference ontologies to standardize descriptions of phenotypes and to facilitate their comparison. The scientific literature is largely under-exploited, although extremely rich in phenotype descriptions associated with cultivars and genetic information. In this paper we propose the Wheat Trait Ontology (WTO) that is suitable for the extraction and management of scientific information from scientific papers, and its combination with data from genomic and experimental databases. We describe the principles of WTO construction and show examples of WTO use for the extraction and management of phenotype descriptions obtained from scientific documents.

Mal-differentiation of Stem Cells: Cancer and Ageing (줄기세포의 분화 결손으로 인한 노화와 암화)

  • Lee, Mi-Ok;Cha, Hyuk-Jin
    • KSBB Journal
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    • v.26 no.3
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    • pp.183-188
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    • 2011
  • Adult stem cells, which have characteristic of self-renewal and multipotency, are specialized cell types, responsible for the tissue regeneration of the damaged tissue. Recent studies suggest that stem cells senescence (or stem cells' ageing) is closely associated with the variety of ageing-related phenotypes such as tissue atrophy, degenerative diseases and onset of cancers. During ageing, declining of stem cells function and subsequently occurring mal-differentiation of stem cells would be important to understand the biological process of development of ageing-related phenotypes such as tissue degenerations and cancers. This review focuses on the DNA damage stress as a cause of senescence of stem cells and their mal differentiation, which is closely link to defect of regeneration potentials and neoplastic transformation. Understanding of molecular mechanisms governingsuch events is likely to have important implications for developing novel avenues for balancing tissue homeostasis longer period of time, further leading to 'Healthy ageing'.

A Database of Caenorhabditis elegans Locomotion and Body Posture Phenotypes for the Peripheral Neuropathy Model

  • Chung, Ki Wha;Kim, Ju Seong;Lee, Kyung Suk
    • Molecules and Cells
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    • v.43 no.10
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    • pp.880-888
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    • 2020
  • Inherited peripheral neuropathy is a heterogeneous group of peripheral neurodegenerative disorders including Charcot-Marie-Tooth disease. Many peripheral neuropathies often accompany impaired axonal construction and function. To study the molecular and cellular basis of axon-defective peripheral neuropathy, we explore the possibility of using Caenorhabditis elegans, a powerful nematode model equipped with a variety of genetics and imaging tools. In search of potential candidates of C. elegans peripheral neuropathy models, we monitored the movement and the body posture patterns of 26 C. elegans strains with disruption of genes associated with various peripheral neuropathies and compiled a database of their phenotypes. Our assay showed that movement features of the worms with mutations in HSPB1, MFN2, DYNC1H1, and KIF1B human homologues are significantly different from the control strain, suggesting they are viable candidates for C. elegans peripheral neuropathy models.

Association of a Provisional New emm Type Opacity Factor-Negative Group A Streptococci Strain ST4529 with Septicemia

  • R.R. Rantty;M. Eshaghi;A.M. Ali;F. Jamal;K. Yusoff
    • Journal of Microbiology
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    • v.39 no.3
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    • pp.236-239
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    • 2001
  • Group A Streptococcus strain ST4529 is a provisional new ems type which has been recently reported in Malaysia (Jomal, et al. 1999. Energ. Infect . Dis. 5,10-14). This strain was found to be opacity factor (OF) negative with a Tl phenotype. Usually, OF negative strains with T1 phenotypes are associated with acute rheumatic fever. However, strain ST4529 was isolated from the blood of a patient with septicemia. Comparison of the deduced amino acid sequence of the mature hypervariable N-terminus of ST4529 showed only 43% identity with that of M5, the closest matched OF negative strain with a T1 phenotype. Thus, ST4529 most probably encodes a new serospecifically unique M protein which is associated with septicemia rather than pharyngitis infections. The strains with these phenotypes are very important because their sequences should be considered for developing any anti-streptococcal vaccines.

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Genetic studies of Baculovirus used as a microbial pesticide

  • Lee, Hyung-Hoan
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1979.10a
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    • pp.248.1-248
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    • 1979
  • Sixteen temperature-sensitive mutants of Autographa californica nuclear polyhedrosis virus were isolated. Several interesting phenotypes were observed. A large proportion of the mutants were un-able to form polyhedral occlusion bodies at the nonpermissive temperature (32.5C). At 32.5C, one mutant formed plaques in which the cells lacked polyhedra. Another mutant type was defective in the production of progeny extracellular nonoccluded virus and produced a plaque consisting of only a single cell containing polyhedra at 32.5C. One mutant was defective in plaque formation, progeny nonocluded virus formation, and polyhedra formation at 32.5C. Several mutants produced nonocluded virus but failed to produce plaques or polyhedra at 32.5C. Other phenotypes were also distinguished. Complementation analyses, performed by either measuring the increase in extracellular non-ocludedvirus formation or by oberving polyhedra formation in mixed infections at 32.5C, indicated the presence of 15 complementation groups. A high frequency of recombination was observed. Four of the mutants were found to be host dependent in their temperature sensitivity for polyhedra formation.

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Acoustic-Phonetic Phenotypes in Pediatric Speech Disorders;An Interdisciplinary Approach

  • Bunnell, H. Timothy
    • Proceedings of the KSPS conference
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    • 2006.11a
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    • pp.31-36
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    • 2006
  • Research in the Center for Pediatric Auditory and Speech Sciences (CPASS) is attempting to characterize or phenotype children with speech delays based on acoustic-phonetic evidence and relate those phenotypes to chromosome loci believed to be related to language and speech. To achieve this goal we have adopted a highly interdisciplinary approach that merges fields as diverse as automatic speech recognition, human genetics, neuroscience, epidemiology, and speech-language pathology. In this presentation I will trace the background of this project, and the rationale for our approach. Analyses based on a large amount of speech recorded from 18 children with speech delays will be presented to illustrate the approach we will be taking to characterize the acoustic phonetic properties of disordered speech in young children. The ultimate goal of our work is to develop non-invasive and objective measures of speech development that can be used to better identify which children with apparent speech delays are most in need of, or would receive the most benefit from the delivery of therapeutic services.

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Morphological Characters of Panicle and Seed Mutants of Rice

  • Kang Sang-Gu;Pandeya Devendra;Kim Sung-Soo;Suh Hak-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.4
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    • pp.348-355
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    • 2006
  • Phenotypes of panicle, hull and seed of mutant rice (Oryza sativa L.) were characterized. Panicle mutants were classified in 4 groups with their internode length of main rachis, primary rachis, secondary rachis and pedicel. Hull and seed mutants were grouped into 12 based on their mutant characters in shape, size and color of seeds. These natural and spontaneous mutant collections showed distinct phenotypes to wild type rice. This might be useful for the identification of the functions of genetic factors in the Mendelian inheritance.

The cAMP/Protein Kinase A Pathway and Virulence in Cryptococcus neoformans

  • Kronstad, James W.;Hu, Guang-Gan;Choi, Jae-Hyuk
    • Mycobiology
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    • v.39 no.3
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    • pp.143-150
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    • 2011
  • The basidiomycete fungus Cryptococcus neoformans is an important pathogen of immunocompromised people. The ability of the fungus to sense its environment is critical for proliferation and the generation of infectious propagules, as well as for adaptation to the mammalian host during infection. The conserved cAMP/protein kinase A pathway makes an important contribution to sensing, as demonstrated by the phenotypes of mutants with pathway defects. These phenotypes include loss of the ability to mate and to elaborate the key virulence factors capsule and melanin. This review summarizes recent work that reveals new targets of the pathway, new phenotypic consequences of signaling defects, and a more detailed understanding of connections with other aspects of cryptococcal biology including iron regulation, pH sensing, and stress.

Genetic Variation of Hemoglobin and Ganglioside Monooxygenase in Korean Sapsarees (Canis familiaris)

  • Kim, Jong-Bong;Yuichi Tanabe
    • Journal of Life Science
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    • v.10 no.2
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    • pp.14-16
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    • 2000
  • Genetic variation of hemoglobin and erythrocyte ganglisoside monooxygenase were analyzed in Korean nature dog Sapsarees by horizontal starch gel electrophoresis and thin layer chromatography. Hemoglobin has three phenotypes A, B and AB, which are controlled by two codominant alleles, {TEX}$Hb^{a}${/TEX} and {TEX}$Hb^[b}${/TEX}, at one autosomal locus. Gene frequencies of {TEX}$Hb^{a}${/TEX} and {TEX}$Hb^{b}${/TEX} were 0.537 and 0.4625. Ganglioside monooxygenase has two phenotypes, dominant and recessive. They are controlled by a dominant allele {TEX}$Gmo^{a}${/TEX} and a recessive allele {TEX}$Gmo^{g}${/TEX}. Frequencies of {TEX}$Gmo^{a}${/TEX} and {TEX}$Gmo^{g}${/TEX} were 0.5477 and 0.4523.

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