• 한국식품영양학회지
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• 제7권4호
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• pp.332-337
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• 1994

The antioxidant activity of solvent extracts isolated from barley leaves was investigated by measuring peroxide value. The fractions of methanol extract obtained from preparative TLC was also studies, with UV-Visible spectrum, total phenol contents and hydrogen donating ability(HDA) The antioxidant activity of various solvent extracts was, in decreasing order, methanol> ethyl ether> methylene chloride $\geq$ ethyl acetate $\geq$acetone> hexane. The antioxidant activity of the fractions of methanol extract was, in decreasing order, fraction 2> fraction 3> fraction 1 and their activity was all superior to that of tocopherol at 500 ppm level. All fraction(1, 2 and 3) exhibited a strong UV absorption at 280 m which would be specifically produced by phenolic compound. UV absorption at 280 m of fraction 2 was greater than those of fraction 1 and 3. In the visible spectrum of these fractions, the maximum .absorption wavelengths of fraction 1, 2 and 3 were 660, 460 and 460 m, respectively. Antioxidant activity of barley leaves seemed to be due to the flavonoids containing phenolic group by UV spectrum and total phenol content.

• Journal of Ginseng Research
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• 제33권3호
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• pp.194-198
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• 2009

본 연구에서는 수삼을 식품에 쓰이는 산화 방지제인 ascorbic acid와 citric acid로 처리하여 홍삼을 제조한 후 활성성분인 페놀화합물과 진세노사이드의 추출물내 함량 변화를 HPLC를 이용하여 살펴보았다. 분석결과 citric acid 처리 홍삼에서 esculetin과 quercetin 함량이 무처리 홍삼에 비하여 각각 3.5 배, 2.0 배 증가하였고 ginsenoside 함량 역시 citric acid 처리시의 Rg$_3$, Rd, Rh$_2$ 증가량이 ascobic acid 처리시에 비하여 높았다. 따라서 인삼추출물의 이들 특정활성성분 강화를 위하여는 citric acid 처리가 효과적인 것으로 판단된다.

• 한국식품저장유통학회지
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• 제25권1호
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• pp.117-123
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• 2018

이팝나무 꽃으로부터 phenolic 화합물을 추출하기 위하여 열수와 90% 에탄올로 phenolic compounds를 추출하였다. Xanthine oxidase 저해 효과를 측정한 결과, 열수와 에탄올 추출물 $200{\mu}g/mL$ phenolics 농도에서 각각 25.60, 15.92%의 저해 효과를 나타내었다. ${\alpha}$-Glucosidase 저해 효과를 측정한 결과 $200{\mu}g/mL$ phenolics 농도의 에탄올 추출물에서 100.00%의 매우 높은 저해 효과를 나타내었다. 미백효과를 측정하는 tyrosinase 저해 효과를 측정한 결과, 열수와 에탄올 추출물 $200{\mu}g/mL$ phenolics 농도에서 각각 17.27, 36.13%의 저해 효과를 나타내었다. 주름개선 효과를 측정하는 collagenase, elastase 저해 효과를 측정한 결과 에탄올 추출물에서는 $200{\mu}g/mL$ phenolics 농도에서 각각 96.26, 35.93%의 저해 효과를 나타내었다. 항염증 효과를 측정하는 hyaluronidase 저해 효과를 측정한 결과, 열수와 에탄올 추출물 $200{\mu}g/mL$ phenolics 농도에서 각각 36.96, 88.70%의 저해 효과를 나타내었다. 따라서 이팝나무 꽃에서 분리된 phenolic compounds는 $50-200{\mu}g/mL$ phenolics 농도 범위에서 항통풍, 당분해 억제, 미백, 주름개선 및 항염증과 관련된 생리활성 효소를 농도 의존적으로 유의하게 저해하는 것을 확인하였고, 이를 이용한 건강기능성 식품 및 미용식품의 기능성 소재로 활용 가능하다고 판단되었다.

• Journal of Ginseng Research
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• 제41권3호
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• pp.428-433
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• 2017

Background: In this study, the fermentation of ginseng seeds was hypothesized to produce useful physiologically-active substances, similar to that observed for fermented ginseng root. Ginseng seed was fermented using Bacillus, Pediococcus, and Lactobacillus strains to extract ginseng seed oil, and the extraction yield, color, and quantity of phenolic compounds, fatty acids, and phytosterol were then analyzed. Methods: The ginseng seed was fermented inoculating 1% of each strain on sterilized ginseng seeds and incubating the seeds at $30^{\circ}C$ for 24 h. Oil was extracted from the fermented ginseng seeds using compression extraction, solvent extraction, and supercritical fluid extraction. Results and Conclusion: The color of the fermented ginseng seed oil did not differ greatly according to the fermentation or extraction method. The highest phenolic compound content recovered with the use of supercritical fluid extraction combined with fermentation using the Bacillus subtilis Korea Food Research Institute (KFRI) 1127 strain. The fatty acid composition did not differ greatly according to fermentation strain and extraction method. The phytosterol content of ginseng seed oil fermented with Bacillus subtilis KFRI 1127 and extracted using the supercritical fluid method was highest at 983.58 mg/100 g. Therefore, our results suggested that the ginseng seed oil fermented with Bacillus subtilis KFRI 1127 and extracted using the supercritical fluid method can yield a higher content of bioactive ingredients, such as phenolics, and phytosterols, without impacting the color or fatty acid composition of the product.

• Food Science and Biotechnology
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• 제16권1호
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• pp.90-98
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• 2007

The antioxidant activities of water ($H_2O$) and ethanol (EtOH) extracts from hamcho (Salicornia herbacea L.) juice and cake prepared by enzymatic treatments were evaluated by in vitro assays against DPPH, superoxide, and hydroxyl radicals. Among the $H_2O$ and EtOH extracts from five different carbohydrases treated, the EtOH extract from viscozyme-treated hamcho cake had higher yield and phenolic content, and exhibited the strongest radical scavenging activity against DPPH ($IC_{50}=186.91\;{\mu}g/mL$), superoxide ($IC_{50}=87.54\;{\mu}g/mL$), and hydroxyl radicals ($IC_{50}=367.07\;{\mu}g/mL$). Antioxidant assay-guided fractionation and purification of the EtOH extract led to isolation and identification of five phenolic compounds, procatechuic, ferulic and caffeic acids, quercetin, and isorhamnetin. Most of these phenolic compounds exhibited considerable DPPH, superoxide, and hydroxyl radical scavenging activities, and in particular, caffeic and ferulic acids had stronger superoxide and hydroxyl radical scavenging activities than the well-known antioxidant radical scavenger, (+)-catechin (p<0.05). Quercetin and isorhamnetin were the primary compounds responsible for the strong antioxidant activity in the EtOH extract of the viscozyme-treated hamcho cake. Meanwhile, these five phenolic compounds were detected in the EtOH extract of the viscozyme-treated hamcho cake at the following levels (dry base of hamcho); procatechuic acid (1.54 mg%), caffeic acid (6.87 mg%), ferulic acid (8.45 mg%), quercetin (12.63 mg%), and isorhamnetin (6.65 mg%). However, three of these phenolic compounds (procatechuic, caffeic acid, and ferulic acids) were detectable in the $H_2O$ extract of viscozyme-treated hamcho juice. These results suggest that the EtOH extract of viscozyme-treated hamcho cake may be a potential source of natural antioxidants.

• Advances in Traditional Medicine
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• 제8권2호
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• pp.135-145
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• 2008

The study was aimed at evaluating the antioxidant and free radical scavenging activities of ethanolic extract and its fractions of Cleome rutidosperma. The antioxidant activity, reducing power, total phenolic content, total flavonoid content, superoxide anion scavenging activity, nitric oxide anion scavenging activity, in vitro antilipid peroxidation activity and in vitro non-enzymatic hemoglobin glycosylation were studied. The results obtained in the study indicate that Cleome rutidosperma is a potential source of natural antioxidant. All the parameters were found to be concentration dependent and increased with increasing amounts of sample. Flavonoids, phenolic compound like tannins, terpenoids may be responsible for the antioxidant activity of the plant. Variation of solubility parameters in various models may be attributed to non-linearity of activity of ethanol extract fractions models. Further investigation on the isolation and identification of antioxidant component(s) in the plant may lead to chemical entities with potential for clinical use.

• Advances in Traditional Medicine
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• 제8권3호
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• pp.260-265
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• 2008

Annona reticulata belonging to the family Annonaceae is traditionally used as anthelmintic and it is reported to have anticancer activity. Free radicals are known to be involved in various types of diseases like cancer, diabetes, neurological disorders etc. The present study was aimed to evaluate free radical scavenging property of methanol extract of the leaves of Annona reticulata in some in vitro models e.g. DPPH, nitric oxide, superoxide and lipid peroxide radical model. The extract showed good dose-dependent free radical scavenging property in all the models. $IC_{50}$ values were found to be 3.22, 170.01, 25.12 and $140.12\;{\mu}g/ml$ respectively in DPPH, nitric oxide, superoxide and lipid peroxidation inhibition assays. Reductive ability of the extract was also tested based on potassium ferricyanide reduction where dose dependant reducing capability was observed. Measurement of total phenolic compounds by Folin-Ciocalteu's phenol reagent indicated that 1mg of the extract contained $146.20\;{\mu}g$ equivalent of pyrocatechol. The findings ascertain promising free radical scavenging property of the extract and the antioxidant property of the extract may be due to the high content of phenolic compounds.

• 한국자원식물학회지
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• 제30권6호
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• pp.686-692
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• 2017

The aim of this study was to determine the suitability of surfactant to extract higher phenolic compound, flavonoid and antioxidant activity from Tartary buckwheat and evaluate the potentiality of surfactant as a screening agent for breeding purpose. Primarily, we employed two types of surfactant (Hydrophilic: Tween 20 and Lipophilic: Span 80) to select the suitable surfactant agent for the extraction of optimum bioactive compounds. Between two surfactants, Tween 20 showed highest efficiency at 4 mM concentration to extract total phenolic content (TP), total flavonoid (TF) and antioxidant activity (AA). Tween 20 at 4 mM concentration was fixed for further analysis along with hot water ($90^{\circ}C$) treatment as a control. In our findings, highest TP (118 mg/g), TF (38 mg/g) and AA (76%) was achieved in KW21 and KW22 among the fifteen accessions of Tartary buckwheat. In other way, TP, TF and AA was 200%, 120% and 110% higher in surfactant formulation compared with control treatment, respectively.

• 한국작물학회지
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• 제51권spc1호
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• pp.215-219
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• 2006

전국에서 수집한 쑥들에 대한 항산화력과 항산화성분 함량을 비교 분석하여 유효 약용성분을 다량 함유하는 우량계통을 선발한 후 대량 재배하였고, 한방건강식품으로 쑥차를 개발하였다. 개발된 쑥차의 우수성을 알아보기 위해 시판되고 있는 차들을 구입하여 항산화력과 항산화성분 함량을 비교 분석하는 실험들을 수행하였다. 수집 선발한 쑥 AC60, AC67, AC77을 원료로 하여 만든 차들과 시판 차들의 총 폴리페놀 함량들을 분석한 결과 AC67의 총 폴리페놀 함량이 109.6 mg/100 mL로 가장 높았다. 선발 쑥차들의 아질산염 소거능은 선발 쑥 중에서는 AC67가 PH 1.2에서 92.5%로 가장 높았고, 시판 차들 중에는 쟈스민이 98.1%, 감잎차가 96.4%, 녹차가 98.5%, 작설차가 98.7%로 매우 높은 값들을 보여 주었다. SOD 유사 활성을 비교해 본 결과 선발 쑥을 원료로 하는 차들에서는 AC67가 91.7%, AC77이 93.7%로 높았고, 시판 차들에서는 설록차가 92.1%, 루이보스티가 93.8%로 높게 나왔다. 이 결과는 선발 쑥 AC60, AC67, AC77 등은 시판 중인 설록차, 자스민, 작설차, 녹차, 루이보스티 및 페퍼민트차 등과 같은 수준의 고급차의 제조에 이용성이 높을 것으로 생각되었다.

• 한국식품영양과학회지
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• 제39권12호
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• pp.1819-1825
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• 2010

인삼정과 제조 시 이용되는 당 용액 대신에 올리고당 함량이 높은 맥문동의 열수추출액을 이용하여 인삼정과를 제조한 후 품질 특성을 조사하였다. 맥문동 추출액으로 제조한 인삼정과는 가용성 무질소물인 탄수화물의 함량이 76.40%로 설탕으로 제조한 인삼정과의 80.01%에 비하여 약 4% 정도 낮았고, 조단백질 및 조회분 함량은 각각 1.46% 및 3.49%로 일반인삼정과의 0.59% 및 0.96%에 비하여 약 3배 이상 높았다. 맥문동 열수추출액을 이용한 인삼정과에서 유리당 조성 및 함량은 fructose가 32.42%로 가장 많았고, 그 외 올리고당이 16.91%, 설탕 및 이당류가 13.91%, glucose가 13.16%이었으며, Rh2를 비롯한 진세노사이드 11종이 검출되어 총 함량이 740.1 mg%로 일반 인삼정과(675.6 mg%)보다 더 높았다. 총 페놀화합물과 플라보노이드 함량은 각각 5.46과 0.016%로 일반인삼정과의 5.02와 0.014%보다 높았다. 맥문동 열수추출액으로 제조한 인삼정과의 항산화활성은 DPPH $IC_{50}$값이 인삼정과 중량기준으로 약 34.5 mg/mL 이었으며, hydrogen peroxide 소거능은 $50{\mu}g$/mL의 농도에서 92%의 소거능을 보여주었다.