• 약학회지
• /
• 제26권2호
• /
• pp.129-132
• /
• 1982

A GC/MS method was developed to analyze phenolic acid extract from tobacco leaf. Extracted acids were converted to their methyl esters by refluxing with 3M hydrogen chloride in methanol, and the esters were reacted with his (trimethylsilyl) trifluoroacetamide plus 10% trimethylchlorosilane to silylate the phenolic groups. Derivatives of standard salicylic, p-hydroxybenzoic, vanillic, gentisic, p-coumaric, syringic, ferulic, and sinapic acids prepared by this procedure were analyzed by GC/MS on $20m{\times}0.2mm$ column of SE-54 glass capillary. GC/MS analysis of the extract from tobacco leaf revealed the presence of salicylic, p-hydtoxybenzoic, vanillic, gentisic, protocatechuic, p-coumaric, syringic, gallic, ferulic, caffeic, sinapic, and quinic acids, respectively. The quantitative analysis of these phenolic acids were achieved by using multiple ion selection technique.

• Bulletin of the Korean Chemical Society
• /
• 제31권12호
• /
• pp.3755-3759
• /
• 2010

A on-line SPE (solid phase extraction)-HPLC preconcentration method was developed for the determination of phenolic compounds at trace levels in environmental water sample. XAD-4 and Dowex 1-X8 were used as sorbent in the on-line SPE-HPLC method for the selective enrichment of nine phenolic compounds, which are included in the priority pollutants list of the US EPA. Also alumina prefiltering considerably reduced the amount of interfering peaks due to humic substances that could accumulated due to the preconcentration step and prevent quantification of polar phenolic compounds in environmental water samples. This method was used to determine the phenolic compounds in tap and river water and superiority to the US EPA 625 method in its enrichment factor, pretreatment time, recoveries, and detection limit. The limits of detection were in the range of $0.3-0.9\;{\mu}g/L$ in tap water sample.

• 한국식품과학회지
• /
• 제8권2호
• /
• pp.80-84
• /
• 1976

Lasoda와 Sebago 두 종류의 감자를 사용하여, 조리 조건을 달리 하여 conventional oven에 $218^{\circ}C$에서와 microwave oven에서 구어내어 껍질을 베끼고 cortex 부분만 취하여 전체 phenol을 분석하였다. 또한 chlorogenic acid와 caffeic acid를 표준으로 하여 thin-layer chromatography로 감자의 phenol화합물을 분석 하였다. Phenol양은 구운 감자보다 생 감자에 더 많았고, Sebago 보다 Lasoda에 많았다. 수분손실은 conventional oven에서 구운 감자에 컸고, microwave oven에서 구운 Lasoda에는 phenol양이 많았다. 또한 phenol양이 많은 Lasoda가 chlorogenic acid도 많았다.

• Preventive Nutrition and Food Science
• /
• 제4권4호
• /
• pp.221-225
• /
• 1999

Antioxidative compounds contained in roasted safflower seeds were investigated. Six phenolic compounds, N-feruloylserotonin, N-(p-coumaroyl) serotonin, matairesinol, 8'-hydroxyarctigenin, acacetin 7-Ο-β-D-glucoside(tilianine) and acacetin were isolated and identified from the extract of seeds. The inhibitory effects of six phenolic compounds on 1,1-dipheny1-2-picrylhydrazyl(DPPH) radical and lipid peroxidation induced by H₂O₂/FeSO₄in rat liver microsomes were determined. Two serotonins showed more potent DPPH radical scavenging activity, and a stronger inhibitory effect on the lipid peroxidation than that of α-tocopherol. In addition, acacetin and matairesinol also considerably inhibited lipid peroxidation, while 2-hydroxy-arctigenin and tilianine were inactive. These results suggest that phenolic compounds, including serotonins, lignans and flavonoids in the roasted safflower seeds can be used as potential dietary natural antioxidants.

• Carbon letters
• /
• 제17권1호
• /
• pp.33-38
• /
• 2016

In the present study, exfoliated graphite nanoplatelets (xGnP) with different particle sizes were coated onto polyacrylonitrile-based carbon fibers by a direct coating method. The flexural properties, interlaminar shear strength, and the morphology of the xGnP-coated carbon fiber/phenolic matrix composites were investigated in terms of their longitudinal flexural strength and modulus, interlaminar shear strength, and by optical and scanning electron microscopic observations. The results were compared with a phenolic matrix composite counterpart prepared without xGnP. The flexural properties and interlaminar shear strength of the xGnP-coated carbon fiber/phenolic matrix composites were found to be higher than those of the uncoated composite. The flexural and interlaminar shear strengths were affected by the particle size of the xGnP, while the particle size had no significant effect on the flexural modulus. It seems that the interfacial contacts between the xGnP-coated carbon fibers and the phenolic matrix play a role in enhancing the flexural strength as well as the interlaminar shear strength of the composites.

• 생약학회지
• /
• 제41권4호
• /
• pp.308-312
• /
• 2010

The purpose of this research is to provide basic informations to discriminate between sea buckthorn (Hippophae rhamnoides) cultivated in the Republic of Korea and Mongolia. The phenolic compounds of sea buckthorn, were measured by the HPLC analysis. Catechin, rutin, quercetin, isorhamnetin were found in methanol extracts of sea buckthorn. Total phenolic compound of Korean sea buckthorn leaves (1.852%) was about five times higher than those of Mongolian sea buckthorn berries (0.338%). As a result, the order of the total phenolic compound and Catechin content was 1) sea buckthorn leaves, 2) sea buckthorn stems, 3) sea buckthorn roots, and 4) sea buckthorn berries. Statistically no big differences in levels of phenolic compounds were consistently found in sea buckthorn cultivated in the Republic of Korea and Mongolia investigated in this work.

• 생약학회지
• /
• 제38권2호통권149호
• /
• pp.164-169
• /
• 2007

ln our continued search for biologically active compounds from traditional medicine, we found that ethyl acetate fraction from Inonotus obliquus showed potent antioxidant activities on three different cell-free bioassay systems. Bioassay-directed chromatographic fractionation of the ethyl acetate fraction from Inonotus obliquus afforded four phenolic compounds, 4-(3,4-dihydroxyphenyl)-(E)-3-buten-2-one (1) , 3,4-dihydroxybenzaldehyde (2), protocatechuic acid (3) and caffeic acid (4) along with three sterols such as lanosterol (5), ergosterol peroxide (6) and 9,11-dehydroergosterol peroxide (7). Among isolates, phenolic compounds (1-4) were assessed for antioxidant activities. Almost phenolic compounds showed potent DPPH and superoxide anion radicals scavenging and lipid peroxidation inhibitory activities indicating that these phenolic compounds contribute to the antioxidative activities of I. obliquus. Compounds 2-3 and 7 were isolated for the first time from this plant.

• Journal of Ecology and Environment
• /
• 제36권1호
• /
• pp.57-63
• /
• 2013

This study compared the antimicrobial activity and total phenolic content of three Pinus plants (Pinus densiflora, P. thunbergii, P. rigida) for the first time. The antimicrobial activity of the water fraction of methanol extract of fresh leaves was stronger than that of fallen leaves at any concentrations. The water fraction of crude methanol extract from fresh leaves of P. thunbergii showed a higher growth inhibitory activity against gram-positive and gram-negative bacteria than that of P. densiflora and P. rigida. The results from the disc diffusion method followed by measurements of minimal inhibition concentration (MIC) indicate that Bacillus subtilis was the most sensitive microorganism with the lowest MIC value. The highest total phenolic content was found in fresh leaves of P. rigida and P. thunbergii. The assay showed that the fresh leaves of the three Pinus plants contained higher total phenolic content than fallen leaves of the three plants. The antimicrobial activity was related with the total phenolic content.

• 한국정보통신학회논문지
• /
• 제15권4호
• /
• pp.1000-1006
• /
• 2011

본 연구에서는 vir유전자의 발현에 있어서 페놀화합물, Ti 플라스미드들의 종류(cctopine, nopaline), A. tumefaciens 들의 영향에 대해서 조사하였다. 9종류의 페놀화합물들을 3종류의 A. tumefaciens들과 3종류의 Ti 플라스미드들을 대상으로 조사하였다. Nopaline Ti 플라스미드를 포함하는 A. tumefaciens MW107에 존재하는 vir유전자는 4-hydroxyacetophenone, phenol, catechol, resorcinol, acetosyringone과 vanillin등 6종류의 페놀화합물들에 의해서 상대적으로 높게 발현되었다. Octopine Ti 플라스미드들을 포함하는 A. tumefaciens MW105와 MW108의 vir유전자들은 acetosyringone에서만 발현되었다. 따라서 vir유전자의 발현을 유도시키는 요인들은 Ti 플라스미드 종류, A. tumefaciens와 페놀화합물들의 종류에 따라서 서로 다르다는 결과를 얻었다.

• 분석과학
• /
• 제14권5호
• /
• pp.377-383
• /
• 2001

사과에 분포하는 페놀계 물질의 총 함량을 분석하는데 가장 적절한 방법을 확립하기 위하여 우리나라에서 재배되는 4품종을 각각 5개 지역에서 수집한 20개 종류의 사과를 시료로 가장 효율적인 분석조건을 조사하였다. 천연재료 줌의 총 페놀 정량방법으로 이용되고 있는 분광광도법인 Foline-Dennis법, Prussian-blue법 그리고 vanillin-HCI법을 각각 시료에 적용하여 총 페놀 함량을 측정하여 고찰하였다. 그 결과 chlorogenic acid 상당량으로 산출한 Folin-Dennis법이 사과의 총 페놀 정량방법으로 가장 적합하였다.