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Antioxidative Activities of Phenolic Compounds Isolated from Inonotus obliquus  

Kim, Hyoung-Ja (Bioanalysis and Biotransformation Research Center, Division of Life Sciences, Korea Institute of Science & Technology, Kyung Hee East-West Pharmaceutical Research Institute, Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University)
Jin, Chang-Bae (Bioanalysis and Biotransformation Research Center, Division of Life Sciences, Korea Institute of Science & Technology, College of Pharmacy, Kyung Hee University)
Lee, Yong-Sup (Kyung Hee East-West Pharmaceutical Research Institute, Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.2, 2007 , pp. 164-169 More about this Journal
Abstract
ln our continued search for biologically active compounds from traditional medicine, we found that ethyl acetate fraction from Inonotus obliquus showed potent antioxidant activities on three different cell-free bioassay systems. Bioassay-directed chromatographic fractionation of the ethyl acetate fraction from Inonotus obliquus afforded four phenolic compounds, 4-(3,4-dihydroxyphenyl)-(E)-3-buten-2-one (1) , 3,4-dihydroxybenzaldehyde (2), protocatechuic acid (3) and caffeic acid (4) along with three sterols such as lanosterol (5), ergosterol peroxide (6) and 9,11-dehydroergosterol peroxide (7). Among isolates, phenolic compounds (1-4) were assessed for antioxidant activities. Almost phenolic compounds showed potent DPPH and superoxide anion radicals scavenging and lipid peroxidation inhibitory activities indicating that these phenolic compounds contribute to the antioxidative activities of I. obliquus. Compounds 2-3 and 7 were isolated for the first time from this plant.
Keywords
antioxidant; hymenochaetaceae; Inonotus obliquus; phenolic compounds; sterols;
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