• 한국자원식물학회지
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• 제25권4호
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• pp.363-371
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• 2012

본 연구에서는 딱총나무의 항산화 활성을 조사해 보았다. 딱총나무 잎과 줄기의 물추출물에는 6.6 mg/g과 2.0 mg/g의 phenolic compound가 함유되어 있었다. 추출물의 항산화 활성 실험에서 전자공여능은 딱총나무 잎의 물과 에탄올 추출물 phenolic 200 ${\mu}g/mL$의 함량에서는 99.5%, 89.7%, 줄기의 물과 에탄올 추출물 phenolic 200 ${\mu}g/mL$의 함량에서는 92.2%, 94.3%의 높은 항산화 효과를 나타내었다. 또한 ABTS radical cation decolorization 측정에서도 딱총나무 잎의 물과 에탄올 추출물 phenolic 200 ${\mu}g/mL$의 함량에서는 79.8%, 99.1%, 줄기의 물과 에탄올 추출물 phenolic 200 ${\mu}g/mL$의 함량에서 90.8%, 97.2%의 매우 높은 항산화 효과를 나타내었다. Antioxidant protection factor에서는 딱총나무 잎의 물과 에탄올 추출물 phenolic 200 ${\mu}g/mL$의 함량에서는 1.1과 1.1 PF, 줄기의 물과 에탄올 추출물 phenolic 200 ${\mu}g/mL$의 함량에서는 1.4와 1.0 PF의 항산화효과를 나타내었다. Thiobarbituric acid reaction substance 측정에서도 딱총나무 잎의 물과 에탄올 추출물 phenolic 200 ${\mu}g/mL$의 함량에서는 88.7%, 98.1%, 줄기의 물과 에탄올 추출물 phenolic 200 ${\mu}g/mL$의 함량에서는 93.6%, 90.6%로 positive control인 BHT 및 vitamin C보다 우수한 항산화 효과를 나타내었다. 본 연구의 결과로 딱총나무의 항산화 활성을 이용한 천연 기능성 식품소재로서의 활용이 가능할 것으로 판단되었다.

• Journal of Ginseng Research
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• 제26권3호
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• pp.165-169
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• 2002

고려인삼의 적변내성 지표성분을 선발하기 위하여 적변지수가 낮아 적변에 강한 건전계통(LRL) 7계통과 적변지수가 높아 적변에 약한 적변계통(HRL) 7계통을 선발하여 시료로 사용하였다. 4년생과 6년생의 적변지수 상관관계는 고도의 유의성을 나타내었다. phenolic compounds 함량이 중심주 및 지세근 조직에서는 차이가 없었다. 피층조직에서는 적변인삼은 건전인삼보다 phenolic compounds 성분 함량이 높았으나, 적변의 정도를 동일하게 한적변계통군와 건전계통군간에는 phenolic compounds 함량의차이가 없어서 지표성분으로 활용 가능성이 없었다. 표피조직에서 적변삼은 건전삼에 비해 phenolic compounds 함량이 높았고, 적변삼이나 건전삼을 시료로 사용 했을 때 모두 적변계통군(HRL)은 건전계통군(LRL)보다 phenolic compounds 함량이 높아서, 적변삼 선발 지표성분으로 활용 가능성이 제시되었다.

• 대한본초학회지
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• 제21권2호
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• pp.143-150
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• 2006

Oriental pear was used as treatment of asthma, control of blood pressure tonic medicine diabetes in oriental medicine, Pear Pectin was effective on control of blood pressure in previous report. In this study, it was investigated that Woohwang with pear extractions effects on cardiovascular system as blood pressure and renin and Atrial natriuretic peptide(ANP) in plasma. The 2K1C hypertension model was prepared by constricting the left renal artery with a sliver dip. Animals were then divided into three groups, control, Woowhang:Pear Phenolic compound(1:1), Woowhang:Pear Phenolic compound(2:1),Woowhang:Pear Phenolic compound(1:2) were supplied with them. Direct-blood pressure was measured at femoral vein, Indirect-blood pressure was measured at rodent tail. The results are as follows. The blood pressure was more significantly decreased at 1:2(woohwang:pear phenolic compound)group than other groups. On 6,9,12,15days, the blood pressure was significantly decreased in 1:2(woohwang:pear phenolic compound)group. The plasma ANP was significantly increased in 1:2(woohwang:pear phenolic compound)group. It tenders to decrease in 1:2(woohwang:pear phenolic compound)group on plasma renin. Based on the above results it is assumed that oral administration of Woohwang with Pear Phenolic compound(1:2) can help the treatment of hypertension.

• Journal of Ginseng Research
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• 제20권3호
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• pp.284-290
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• 1996

To Identify phenolic components known to exist in Korean ginseng (Panax ginseng C.A. Meyer) by GC/MS, three derivatization methods were employed for their analyses. First, phenolic components in ether soluble acidic fraction prepared from Korean red ginseng powder were taimethylsilylated. Secondly, phenolic acids in the same fraction were esterified with diazomethane followed by trlmethylsilylation. Thirdly, acidic components in ginseng powder were extracted and esterified concurrently by methanolic sulfuric acid, followed by fractionation of phenolic components with Silica Sep-Paka and trimethylsilylatlon. All phenolic components found in ginseng except gen tisic acid were identified by retention times and mass spectrums of standards. Besides, 5 phenolic components including salicyl alcohol and 1-H-indole-2-carboxylic acid were first identified from Korea an red ginseng by GC/MS.

• 한국약용작물학회지
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• 제20권6호
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• pp.447-453
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• 2012

This study was conducted to determine the contents of phenolic compounds and astragalosides in Astragalus membranaceus. Between the phenolic compound groups, flavonoids compounds (51.13%) had higher average concentrations than the phenolic acids groups. Among the 30 phenolic compounds, quercetin ($353.11{\mu}g\;g^{-1}$) provided the highest concentrations. According to different cultivation year, 3-year-cultivated Astragalus ($2612.57{\mu}g\;g^{-1}$) showed the highest concentrations of phenolic compounds. According to different harvest days, 6/5 harvesting Astragalus ($2615.80{\mu}g\;g^{-1}$) showed the highest concentrations of phenolic acids. Comparison between the top and subterranean parts of harvested Astragalus plants cultivated for 2 years, 3 years, and 5 years showed big difference of total phenolic compounds in concentrations. Further, the top part had higher amounts of the total phenolic compounds than the subterranean part among all Astragalus. This tendency was similar to those of the top root and lateral root. The concentration of the phenolic compounds in the top root was higher than that of the lateral root.

• Food Science and Biotechnology
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• 제14권3호
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• pp.371-374
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• 2005

The compositions and antioxidant activities of tree and hydrolyzed phenolic acids, which are aglycones of esterified phenolic acids, in wild ginseng leaves were investigated. The contents of tree and hydrolyzed phenolic acids in the wild ginseng leaves were $422.4\;{\pm}\;3.5$ and $319.6\;{\pm}\;5.7\;mg/100\;g$, respectively, as gallic acid equivalents. Free phenolic acids were composed of 55.3% benzoic acid derivatives and 44.6% phenylpropanoids. The major constituents of free phenolic acids in the ginseng leaves were syringic (139.4 mg/l00 g) and sinapic (131.2 mg/100 g) acids. On the other hand, hydrolyzed phenolic acids in the ginseng leaves were mainly composed of caffeic (59.4 mg/100 g), ferulic (49.5 mg/100 g), and p-coumaric (33.8 mg/100g) acids. Phenylpropanoid content was higher (82.7%) than benzoic acid derivatives (17.3%). $IC_{50}$ values of DPPH radical scavenging activity were $10.2\;{\mu}g/mL$ for tree phenolic acids and 8.0 mg/mL for hydrolyzed phenolic acids, as gallic acid equivalents. Hydrolyzed phenolic acids also exhibited higher hydroxyl and superoxide radical scavenging activities than free phenolic acids did. These results indicated that the antioxidant activities of the wild ginseng leaves were correlated more closely with phenylpropanoid contents than with total amount of phenolics.

• Preventive Nutrition and Food Science
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• 제2권4호
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• pp.316-320
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• 1997

Phenolic acids are regarded as harmful materials in food and environment science but recently, as useful materials, and thus adsorption is recommended as an effective separation technique to recover or remove phenolic acids from diluted solution. If the adsorbed phenolic compounds were useful materials, the materials should be recovered through desorption. Desorption using supercritical carbon dioxide(SC-$CO_2$) was tried to separate food-borne phenolic acids from charcoal in single solute system. In the comparisons of desorption amounts, gallic acid had the lowest lolubiligy to SC-$CO_2$. Gallic acid has more hydroxy functional groups than the other phenolic acids, which was immiscible with nonpolar SC-$CO_2$. Ferulic acid was yielded more than p-coumaric acid, because ferulic acid had much bigger molecular weight, which was affected more by van der Waas force. It was found that the most affecting factor on desorption amounts was the solubility of phenolic acids to SC-$CO_2$. The second affecting factor was van der Waals force. Response surface methodology(RSM) was conducted to read the trend of desorption. Increasing density of SC-$CO_2$ raised solubility of phenolic acids.

• 원예과학기술지
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• 제30권1호
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• pp.34-41
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• 2012

본 연구는 새롭게 개발된 무기물인 phenolic foam 배지를 이용한 파프리카 파종용 플러그묘의 생산 가능성을 알아보기 위해 유리온실에서 수행하였다. Grodan 암면, UR 암면, phenolic foam LC, phenolic foam RC, phenolic foam 3813-4의 5종류 펠릿형 배지에 파프리카 'Fascinato'를 파종하였다. 종자는 식물생장상($25^{\circ}C$, 상대습도 90%, 암상태)에서 4-5일 동안 발아시켰고, 발아가 시작된 후에는 유리온실로 옮겨 두상관수로 양액을 관주하였다. 파프리카의 생육은 파종 20일 후에 측정하였다. 배지의 pH를 측정한 결과 Grodan 암면이 가장 높았고, EC는 phenolic foam 3813-4가 가장 높았지만 식물의 독성 증상은 관찰되지 않았다. 모든 배지에서 90% 이상의 발아율을 보였다. 초장, 경경, T/R률, 엽면적, 엽록소 함량은 암면 배지와 비교하였을 때 생육이 유사한 것으로 나타났다. 엽수, 최대근장, 지상부의 건물중은 유의적인 차이가 없었다. 전체적으로 phenolic foam 배지인 LC와 RC는 암면배지와 비교하여 유사한 생육을 보였다. 본 실험결과 파프리카 'Fascinato'의 상업적 규모의 육묘를 위해 암면을 신개발 물질인 페놀릭 폼으로의 대체 가능성을 확인하였다.

• 한국식품과학회지
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• 제25권1호
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• pp.9-14
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• 1993

탈지들깨박으로부터 추출한 유리페놀산, 페놀산 에스터 및 불용성 결합형 페놀산 형태의 페놀화합물들의 항산화효과를 일반 시판용 합성항산화제들과 비교하였다. Chlorogenic acid를 표준물로써 비색법으로 측정한 페놀화합물들의 총함량은 0.75%였고 총페놀산중 유리페놀산, 페놀산 에스터 및 불용성 페놀산 추출물이 차지하는 비율은 각각 87.5, 7.5, 5.0%였다. 이차원 전개법에 의한 paper chromatography 성분분석 결과 유리페놀산 형태의 추출물에서는 대부분의 폐놀화합물이 chlorogenic acid와 일치하였고 소량의 caffeic acid와도 일치되었으나, 페놀산 에스터와 불용성 결합형 페놀산 형태의 추출물에서는 나타나지 않았다. 30g의 탈지들깨 종자박에서 추출된 각 형태의 페놀산 추출물들은 각각의 페놀함량 차이가 큼에도 불구하고 식용대두유 기질에서 0.02%(w/w) 농도로 첨가된 BHT와 비슷한 정도의 항산화 효과를 나타냈다.

• 접착 및 계면
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• 제8권4호
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• pp.14-22
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• 2007

본 연구에서는 페놀수지의 열안정성 그리고 glass braid/페놀수지 복합재료의 동역학적 열특성에 미치는 유리분말 및 액상 니트릴고무(NBR)의 영향을 열중량분석기와 동역학적 열분석기를 이용하여 조사하였다. 수용성 페놀수지에 충진된 유리분말과 NBR은 페놀수지의 열안정성과 복합재료의 저장탄성률 그리고 tan ${\delta}$ 변화에 중요한 영향을 미쳤다. 유리분말의 충진은 페놀수지의 열안정성을 향상시키는 반면, NBR의 존재는 특정 온도영역에서 페놀수지의 중량감소를 초래하였다. 충진제의 유 무에 따른 페놀수지의 열안정성은 경화온도뿐만 아니라 경화시간에도 의존하였다. 스트립형태의 glass braid/페놀수지 복합재료의 저장탄성률과 tan ${\delta}$의 변화는 유리분말과 NBR이 혼합된 매트릭스의 도입과 경화조건에 의해 크게 영향을 받았다.