• Title/Summary/Keyword: Phase Feeding

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Supply of Tryptophan and Tryptamine Influenced the Formation of Melatonin in Viola Plants (제비꽃속(Viola) 식물에서 tryptophan과 tryptamine 공급이 멜라토닌 생성에 미치는 영향)

  • Kim, Yeo-Jae;Yoon, Young-Ha;Park, Woong-June
    • Journal of Life Science
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    • v.21 no.2
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    • pp.328-333
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    • 2011
  • Melatonin has been known as an animal hormone. However, melatonin exists in diverse organisms including higher plants. The biosynthesis and physiological roles for melatonin in plants is still largely unknown, although both dicot and monocot plants have melatonin and some medicinal plants even contain large amounts of melatonin. In this study we detected melatonin in diverse Viola plants, in which melatonin had not been examined so far, by reverse phase HPLC analysis, demonstrating the wide existence of melatonin in the genus of Viola. We then fed tryptophan (Trp) and tryptamine (TAM) to the incubation medium for Viola leaf sections to test their effects on melatonin formation. Trp is also the hypothesized starting material of melatonin in plants, and TAM is the following intermediate produced by the decarboxylation of Trp. Trp feeding did not affect the contents of melatonin. In contrast, TAM feeding clearly increased the level of melatonin in Viola leaves. Because TAM is derived from Trp, we concluded that the Trp-TAM pathway exists in Viola plants as well. Ineffectiveness of Trp feeding to the change of melatonin contents supports the hypothesis that the decarboxylation step from Trp to TAM is the rate-limiting step in plant melatonin biosynthesis.

Effect of biogas production to different anaerobic digestion systems and feeding stocks (혐기소화 공정 및 원료 유형별 바이오가스 생산에 미치는 영향)

  • Shin, JoungDu;Hong, Seung-Gil;Park, Woo-Kyun;Park, SangWon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.19 no.4
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    • pp.66-73
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    • 2011
  • Objective of this study was to investigate the effect of biogas production to different systems and feeding stocks. For the biogas production through operating the temperature phase anaerobic digestion(TPAD) with different feeding stocks, the stage state of biogas production with 70% of methane concentration in the thermophilic digestion tank with co-digestion of food waste and swine manure(40 : 60) was delayed at 3.5 times, but its mesophilic tank was short for 5 days as relative to the swine manure. The cumulative methane production in the thermophilic digestion tank with co-digestion of food waste and swine manure was started with greater than its swine manure at 60 days after digestion periods. However, its mesophilic tank with swine manure was great at 3 days after digestion periods. For aspect of anaerobic digestion processes with swine manure, it was appeared that the stage state of biogas production rate in TPAD was shorter than the two phase anaerobic digestion system.

Growth Performance, Carcass Characteristics and Plasma Mineral Chemistry as Affected by Dietary Chloride and Chloride Salts Fed to Broiler Chickens Reared under Phase Feeding System

  • Mushtaq, M.M.H.;Pasha, T.N.;Akram, M.;Mushtaq, T.;Parvin, R.;Choi, H.C.;Hwangbo, J.;Kim, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.6
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    • pp.845-855
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    • 2013
  • Requirements of dietary chloride (dCl) and chloride salts were determined by using $4{\times}2$ factorial arrangement under four phase feeding program. Four levels (0.31, 0.45, 0.59 and 0.73%) and two sources ($NH_4Cl$ and $CaCl_2$) of the dCl were allocated to 1,472 chicks in eight dietary treatments in which each treatment was replicated four times with 46 birds per replicate. The four phase feeding program was comprised of four dietary phases: Prestarter (d 1 to 10), Starter (d 11 to 20), Grower (d 21 to 33) and Finisher (d 34 to 42); and diets were separately prepared for each phase. The cations, anions, pH, dissolved oxygen (DO), temperature, electrical conductivity (EC), total dissolved solids (TDS) and salinity were analyzed in drinking water and were not affected by dietary treatments. BW gain (BWG; $p{\leq}0.009$) and feed:gain (FG; $p{\leq}0.03$) were improved in $CaCl_2$ supplemented diets during d 1 to 10. The maximum response of BWG and FG was observed at 0.38% and 0.42% dCl, respectively, for d 34 to 42. However, the level of dCl for BWG during d 21 to 33 ($p{\leq}0.04$) and d 34 to 42 ($p{\leq}0.009$) was optimized at 0.60% and 0.42%, respectively. The level of dCl for optimized feed intake (FI; $p{\leq}0.006$), FG ($p{\leq}0.007$) and litter moisture (LM; $p{\leq}0.001$) was observed at 0.60%, 0.38% and 0.73%, respectively, for d 1 to 42. Water intake (DWI) was not affected by increasing dCl supplementation (p>0.05); however, the ratio between DWI and FI (DWI:FI) was found highest at 0.73% dCl during d 1 to 10 ($p{\leq}0.05$) and d 21 to 33 ($p{\leq}0.009$). Except for d 34 to 42 ($p{\leq}0.006$), the increasing level of dCl did not result in a significant difference in mortality during any phase. Blood pH and glucose, and breast and thigh weights (percentage of dressed weight) were improved while dressing percentage (DP) and gastrointestinal health were exacerbated with $NH_4Cl$ as compared to $CaCl_2$ supplemented diets ($p{\leq}0.001$). Higher plasma $Na^+$ and $HCO_3{^-}$ and lower $Cl^-$ and $Ca^{{+}{+}}$ were observed in $NH_4Cl$ supplemented diets ($p{\leq}0.001$). Increasing supplementation of dCl increased plasma $Cl^-$ ($p{\leq}0.04$; quadratically) and linearly reduced plasma $K^+$ ($p{\leq}0.001$), $Ca^{{+}{+}}$ ($p{\leq}0.003$), $HCO_3{^-}$ ($p{\leq}0.001$), and $Na^+$ ($p{\leq}0.001$; quadratically). Consequently, higher requirements of dietary chloride are suggested for feed intake; nevertheless, lower levels of dietary chloride are sufficient to support optimal BWG and FG with increasing age. The $NH_4Cl$ supplemented diets ameliorate breast and thigh meat yield along with overall energy balance (glucose).

Production of L-Tryptophan by Enzymatic Processes (효소공정에 의한 트립토판 생산)

  • 이인영;안경섭;김의환;이선복
    • Microbiology and Biotechnology Letters
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    • v.20 no.1
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    • pp.73-78
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    • 1992
  • - Enzymatic synthesis of L-tryptophan(Trp) using E. coli tryptophanase has been investigated. In order to reduce the substrate inhibition by indole and to increase the product yield of L-tryptophan three different approaches have been made in this work. First, indole was intermittently fed to the reaction mixture in order to control the indole concentration at lower level. When 15 mM of indole was used as a total amount of substrate, conversion yield of 80% has been obtained with intermittent feeding while only 20% of indole was converted into L-tryptophan by conventional batch operation, The second method employed in this work was the use of cyclohexane-phosphate buffer organic two-phase system. In this system, indole was mainly partitioned into the organic-solvent phase and therefore substrate inhibition was expected to be reduced. L-Tryptophan production in organic two-phase system was, however, unexpectedly lower than that obtained in aqueous buffer solution. As a third method cyclodextrins have been added to the aqueous reaction mixture. It was found that the addition of $\beta$-cyclodextrin enhanced the tryptophan synthesis noticeably while $\alpha$-cycfodextrin showed little effect on tryptophan production.

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Efficient Bioreduction of Ethyl 4-chloro-3-oxobutanoate to (S)4-chloro-3-hydrobutanoate by Whole Cells of Candida magnoliae in Water/ n-Butyl Acetate Two-phase System

  • Xua Zhinan;Fang Limei;Lin Jianping;Jiang Xiaoxia;Liu Ying;Cen Peilin
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.1
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    • pp.48-53
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    • 2006
  • The asymmetric biosynthesis of ethyl (S)-4-chloro-3-hydrobutanoate from ethyl 4-chloro-3-oxobutanoate was investigated by using whole cells of Candida magnoliae JX120-3 without the addition of glucose dehydrogenase or $NADP^+/NADPH$. In a one-phase system, the bioconversion yield was seriously affected on the addition of 12.1 g/L ethyl 4-chloro-3-oxobutanoate. In order to reduce this substrate inhibition, a water/ n-butyl acetate two-phase system was developed, and the bioreduction conditions optimized with regard to the yield and product enantiometric excess value. The optimal conditions were as following: water to n-butyl acetate volume ratio of 1:1, 4.0 g DCW/L active cells, 50 g/L glucose and $35^{\circ}C$. By adopting a dropwise substrate feeding strategy, high concentration of ethyl 4-chloro-3-oxobutanoate (60 g/L) could be asymmetrically reduced to ethyl (S)-4-chloro-3-hydrobutanoate with high yield (93.8%) and high enantiometric excess value (92.7%).

Study on Analysis of Operating Characteristics of Motor Block While KTX is Moving at Neutral Section of Kyung-Bu High Speed Line (경부고속선 절연구간에서 KTX 운행중 모터블럭의 동작특성 분석)

  • Choi, Chang Hyun;Lho, Young Hwan
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.64 no.10
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    • pp.1523-1527
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    • 2015
  • Traction power is supplied by three-phase alternating current of 154 kV power grid and electric trains are operated on single phase feeding system. It becomes important to use all the three phases equally and convert them into two-phase electric power (90 degree phase rotation) for traction supply. This is achieved by special transformer from the adjacent traction substation which is separated by a neutral section. Neutral section locations are in front of the substation and between the two substations. The first stage of the Seoul-Busan high-speed railway, design curve radius is larger than 7,000 m and the greatest slope is 25‰. The railway track conditions are evaluated as good enough to install a neutral section at the first stage, but a few factors of coasting operation of the train should be considered at the second stage of Seoul-Busan high-speed railway. The neutral section was located at Kim-cheon substation, which made some neutral section problems produced by the operating train, and the neutral section was moved about 1.5 km to the south toward Dong Dae-gu station due to the track operation condition. Some of the trains which stopped at the existing Kim-cheon Gu-mi station produced another motor block failure after moving the neutral section. In this paper, power quality, system performance and track condition, etc. are suggested to solve the problems.

Effect of Agricultural Byproduct Supplementation on Growth Performance and Blood Parameters of Broiler Chicken: Meta-analysis (농산부산물의 사료첨가급여가 육계 성장효율과 혈액성상에 미치는 영향: 메타분석)

  • Lee, Jaehun;Kim, Do Hyung;Lee, Ji Hong;Kim, Eun Joong;Cho, Sangbuem;Lee, Sang Moo
    • Korean Journal of Poultry Science
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    • v.45 no.2
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    • pp.81-88
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    • 2018
  • The present study investigated effect of supplementation of agricultural byproduct on growth performance and blood parameters of broiler chicken using meta-analysis. Total 11 studies (32 experiments) where broiler feeding trials with agricultural byproducts were included were employed in analysis. All studies were conducted at commercial farm placed in South Korea and under commercial feeding programs. Growth phases were divided into two groups based on slaughter day. For growth performance, body weight, body weight gain, feed intake and feed conversion rate (feed/gain) were investigated. Carcass weight, dressing percentage, liver and spleen weight, blood glucose, total cholesterol and triglyceride, HDL (High density lipoprotein) and LDL (Low density lipoprotein) cholesterol contents in blood were investigated. Body weight of broiler of treatment was significantly increased than the control (P<0.05), regardless of growth phase. However, body weight gain during late growth phase did not show significant difference between control and treatment. Effect evaluated by random effect model on feed intake was not significant. Effect on feed conversion rate during early growth phase was significantly negative (P<0.05). Effects on carcass weight, dressing percentage, liver and spleen weight were not significant in random effect model. Effect on abdominal fat was significantly negative (P<0.05). In blood parameters, significant effects were found at total cholesterol, triglyceride and HDL cholesterol (P<0.05). Effect on HDL cholesterol only was positive effect, and others were negative.

A Smooth LVRT Control Strategy for Single-Phase Two-Stage Grid-Connected PV Inverters

  • Xiao, Furong;Dong, Lei;Khahro, Shahnawaz Farhan;Huang, Xiaojiang;Liao, Xiaozhong
    • Journal of Power Electronics
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    • v.15 no.3
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    • pp.806-818
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    • 2015
  • Based on the inherent relationship between dc-bus voltage and grid feeding active power, two dc-bus voltage regulators with different references are adopted for a grid-connected PV inverter operating in both normal grid voltage mode and low grid voltage mode. In the proposed scheme, an additional dc-bus voltage regulator paralleled with maximum power point tracking controller is used to guarantee the reliability of the low voltage ride-through (LVRT) of the inverter. Unlike conventional LVRT strategies, the proposed strategy does not require detecting grid voltage sag fault in terms of realizing LVRT. Moreover, the developed method does not have switching operations. The proposed technique can also enhance the stability of a power system in case of varying environmental conditions during a low grid voltage period. The operation principle of the presented LVRT control strategy is presented in detail, together with the design guidelines for the key parameters. Finally, a 3 kW prototype is built to validate the feasibility of the proposed LVRT strategy.

PFC Bridge Converter for Voltage-controlled Adjustable-speed PMBLDCM Drive

  • Singh, Sanjeev;Singh, Bhim
    • Journal of Electrical Engineering and Technology
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    • v.6 no.2
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    • pp.215-225
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    • 2011
  • In this paper, a buck DC-DC bridge converter is used as a power factor correction (PFC) converter for feeding a voltage source inverter (VSI) based permanent magnet brushless DC motor (PMBLDCM) drive. The front end of the PFC converter is a diode bridge rectifier (DBR) fed from single phase AC mains. The PMBLDCM is used to drive the compressor of an air conditioner through a three-phase voltage source inverter (VSI) fed from a variable voltage DC link. The speed of the air conditioner is controlled to conserve energy using a new concept of voltage control at a DC link proportional to the desired speed of the PMBLDC motor. Therefore, VSI operates only as an electronic commutator of the PMBLDCM. The current of the PMBLDCM is controlled by setting the reference voltage at the DC link as a ramp. The proposed PMBLDCM drive with voltage control-based PFC converter was designed and modeled. The performance is simulated in Matlab-Simulink environment for an air conditioner compressor load driven through a 3.75 kW, 1500 rpm PMBLDC motor. To validate the effectiveness of the proposed speed control scheme, the evaluation results demonstrate improved efficiency of the complete drive with the PFC feature in a wide range of speed and input AC voltage.

Spectral Analysis of DC Link Ripple Currents in Three-Phase AC/DC/AC PWM Converters (3상 AC/DC/AC PWM 컨버터의 직류링크 리플전류의 주파수 영역 해석)

  • 이동춘;박영욱;석줄기
    • The Transactions of the Korean Institute of Power Electronics
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    • v.7 no.3
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    • pp.244-252
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    • 2002
  • In this paper, do link ripple currents for three-phase ac/dc/ac PWM converters feeding adjustable speed ac machine drives are analysed in a frequency domain. The expression of the harmonic currents is developed by using switching functions of the converter and exponential courier series expansion. The effect of the displacement angle between the switching Periods of line-side converters and motor-side inverters on the dc link ripple currents is Investigated. Also, the influence of asynchronization of PWM is observed. The result of analysis is compared with frequency spectrum which results from PSIM simulation. The proposed analysis technique is useful to understand the principles of P% and to derive an equivalent model of the dc link capacitors in a high frequency range.