• Journal of Korean Society of Forest Science
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• v.101 no.4
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• pp.695-702
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• 2012

The chestnut management standard diagnostic table is developed and would be utilized in order that a manager raising chestnuts checks where the own level of management is and grasps current state of own for the purpose of planning aims and advancing toward a higher level. The developed 'Chestnut management standard diagnostic table' consisted of 3 first classified items, 19 second classified items and 2 product indicator items by the chestnut experts consultative meeting. A survey of 212 farmhouses in 4 major producing area was conducted. Except invalid survey of 53 farmers, 159 farmhouses interviewed were used in analysis. Total score was calculated with sum of each item's score. According to the survey results, average score is 68.0 and Buyeo received the highest score of 69.7 and Suncheon received the lowest score of 61.8 by regional groups. The higher the group in score, the better it is in output per hectare. But the property of 'the ratio of the best products in total products' does not show a statistical correlation. Generally the score of 'management-based evaluation indicator part' and 'management and sale capacity indicator part' in Suncheon was low because of many elderly people. In part of 'manufacturing technology indicator' as Environmentally-Friendly production is progressed in over 70% of four regions, when comes to a disease and insect pest control there are rarely farmhouses having a way of crop dusting.

• Korean journal of applied entomology
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• v.58 no.2
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• pp.143-149
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• 2019

This study was conducted to examine the attractiveness of yellow sticky trap (YST) for insect pests by the angle of inclination of the trap surface. In strawberry farms with high bed system, YSTs were installed to attract insect pests in vertical direction with one surface, horizontal direction with upper and under surface, and angle of $45^{\circ}$ with upper and lower sticky surface. Thrips (Frankliniella occidentalis in dominance) and Sciaridae (Bradysia agrestis in dominance) species were more attracted on upper surface of $45^{\circ}$ angle trap, vertical surface and upper surface of horizontal trap than on lower surface of $45^{\circ}$ angle trap or horizontal trap. Cicadellidae (Empoasca vitis in dominance) species were caught more on vertical surface and upper surface of $45^{\circ}$ angle trap than on other traps. There were no specific trend in capture of aphid (Aphis gossypii in dominance) and white fly (Trialeurodes packardi in dominance) species among traps, probably because of a low density of the pest species.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• Journal of Ecology and Environment
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• v.36 no.2
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• pp.125-129
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• 2013

The study on the major insect pests community of Hibiscus syriacus. There were three orders, seven families, and thirteen species of insects harmful to H. syriacus. When the harmful insects eating H. syriacus were classified according to which part of the flower they were eating, insects eating leaves, which considered of nine species four families and one order, accounted for 69.23 %. Insects sucking juice from leaves and stems comprised 15.38 %, with two species two families and two orders, while insects eating seeds comprised 15.38 %, with two species two families and two orders. Aphis gossypii appeared once per H. syriacus from the fourth week of April to the second week of June, appearing the most at a rate of 69.05 per H. syriacus during the first week of May. Dichocrocis surusalis appeared at a rate of 0.05 per H. syriacus during the third week of April and regularly appeared from the first week of July to the second week of September. Nortacha derogata appeared at a rate of 0.1 per tree during the first week of May, after which it continuously appeared until the third week of October. Anomis privata appeared at a rate of 0.05 per tree during the first week of May and continuously occurred from the fourth week of June to the fourth week of October. Its occurrence was most frequent at 2.30 per tree during the third week of September.

• Korean Journal of Ecology and Environment
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• v.52 no.4
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• pp.394-402
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• 2019

The Agrobacterium tumefaciens mediated gene transfer is widely used to generate genetic transformation of plants and transient assay of temporal exogenous gene expression. Syringe infiltration system into tobacco (Nicotiana benthamiana) leaves is a powerful tool for transient expression of target protein to study protein localization, protein-protein binding and protein production. However, the protocol and technical information of transient gene expression, especially double strand RNA (dsRNA), in tobacco using Agrobacterium is not well known. Recently, dsRNA is crucial for insecticidal effect on destructive agronomic pest such as Corn rootworm. In this study, we investigated the factor influencing the dsRNA expression efficiency of syringe agro-infiltration in tobacco. To search the best combination for dsRNA transient expression in tobacco, applied two Agrobacterium cell lines and three plant vector systems. The efficiency of dsRNA expression has estimated by real-time PCR and digital PCR. As a result, pHellsgate12 vector constructs showed the most effective accumulation of dsRNA in the cell. These results indicated that the efficiency of dsRNA expression was depending on the kind of vector rather than Agrobacterium cells. In summary, the optimized combination of transient dsRNA expression system in tobacco might be useful to in vivo dsRNA expression for functional study and risk assessment of dsRNA.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.2
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• pp.131-136
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• 2005

Beauveria bassiana F-101, which has high toxicity toward Acantholyda parki as well as Thecodiplosis japonensis, was an isolate to develop an alternative control system against the major forest pests. Up to now, in B. bassiana, only one pr1 gene has been isolated and characterized. Therefore, we here reported the identification of a pr1-like gene, which would be a factor of toxicity from B. bassiana F-101. The oligonucleotides for the amplification of the pr1-like gene, were chosen based on the conserved regions of the subtilisin family enzymes, pr1 genes of B. bassiana and Metarhizium anisopliae, and proteinase K of Tritirachium album. The cloned PCR fragment had 1111 bp including 52 bp intron. The deduced Pr1-like peptide showed a low identity with Pr1s of entomopathogenic fungi such as B. bassiana Pr1 (BbPr1) and M. anisopliae Pr1 (MaPr1) as well as the proteinase K of T. album (TaPrK). Instead, the deduced peptide had a substantially high amino acid sequence identity $(>65\%)$ with the serine proteases of Magnaporthe grisea (MgSPM1) and Podospora anserina (PaPspA). These results, therefore, appear to suggest that the putative Pr1-like peptide of B. bassiana F-101 belongs to the subtilisin-like serine protease family and may be a novel gene.

• Journal of Microbiology and Biotechnology
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• v.8 no.4
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• pp.341-346
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• 1998

Optimization for the production of Pest an was followed by the Plackett-Burman Design, using modified Czapek-dox medium as the starting point. At the flask level, $K_2HPO_4$, $MgSO_4{\cdot}7H_2O$, and aeration variables positively affected the Pestan production, DCW (dry cell weight), apparent viscosity, and flocculating activity response. KCI and $FeSO_4{\cdot}7H_2O$ negatively affected the Pestan production, DCW, apparent viscosity, and flocculating activity response. Aeration variable was shown to have a positive effect on only the flocculating activity response among Pestan production, DCW, and apparent viscosity responses. In comparison of the positive and negative variables media conditions, Pestan production and flocculating activity differed by about 9 and 125 times, respectively. In particular, at the jar fermentor level, the aeration variable was the most important factor of the all responses (pestan production, DCW, apparent viscosity, flocculating activity, and anionic charge density). The flocculating activity and apparent viscosity of Pestan were closely related to the molecular chain length and charge density.

• The Korean Journal of Pesticide Science
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• v.13 no.3
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• pp.165-170
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• 2009

Insecticidal efficacies of plant extracts and environmental friendly agro-materials against green peach aphids, two spotted spider mites, and diamondback moths were evaluated in the lab condition. Oxymatrine and matrine, derivatives of Sophora flavescens, were effective to all three tested pest insect species. Selected environmental friendly agro-materials available from the market mainly contained active ingredients of nicotine, azadirachtin, and matrine were all effective to control green peach aphids and two spotted spider mites.

• Journal of Ecology and Environment
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• v.38 no.3
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• pp.367-374
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• 2015

The aim of this study was to assess the occurrence and foraging of social and other wasps and bees in Jirisan National Park (JNP, South Korea), in particular in an apiary. Sixty one traps were placed in the southwestern part of JNP to identify social wasps from July to September 2014, and the damage to the apiary caused by wasps or bees was observed once a month from May to December 2014 between 10 a.m. and 5 p.m. In total, 10 species of Vespidae were collected by trapping. Vespa crabro was the most abundant (245 individuals, 28.3%), followed by Vespa velutina (162 individuals, 18.7%). In the apiary, however, V. velutina was the most frequent species. V. velutina visited a maximum of 167 times a day in September, which corresponded to one visit in 2.5 min. Accordingly, these data are in line with the most serious impact of V. velutina on the apiaries in South Korea. V. simillima was the second most frequent species; both Vespa species hawked honeybees. Even though the occurrence of V. mandarinia was low, this species caused serious damage by mass slaughter of honeybees. The occurrence of V. crabro, V. analis and V. ducalis was quite low and their impact on honeybees was negligible. There have been few reports of V. dybowskii foraging for honeybees, but they are considered to be a new pest because their impact on apiaries is considerable. Most Vespa species attacked the apiary from June to October, with a maximum in September. However, V. velutina visited until November to early December. Vespula species are not more serious pests than Vespa species, but many adults were observed stealing honey from beehives. Polistes, Orancistrocerus, and Bombus species had no impact on honeybee colonies in the apiary.

• Korean Journal of Agricultural Science
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• v.38 no.3
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• pp.413-419
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• 2011

Recently, the rate of cultivation of such as hairy vetch was increased as a way of reducing the current soil problem. However, the occurrence of insect pest such as bean bugs, Riptortus pedestris, were increased in the field of green manure crops and their injuries to hairy vetch were observed. While minimizing insecticide use for the environmental friendly agriculture, the control of the bean bug can be utilized environmental friendly agricultural materials (EFAMs) in the green manure crop fields. The control effects of some EFAMs and most of chemical insecticides to the bean bug were significantly high. As a result of direct spray of EFAM that contained sophora extract or neem extract, the control effects of six EFAMs were higher than 70% at 120 hours after treatments. Among them, three EFAMs were showed over 90% of control effects. On the other hand, most of chemical insecticides were showed 100% of control effect against the bean bug at 48 hours after treatment. As mortality effects of EFAMs were sloely observed until 120h after treatment, we must use selected EFAMs at the beginnibgs of occurrence and entrance in the field of green manure for effective control of bean bugs.