• 동국한의학연구소논문집
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• 제5권
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• pp.1-13
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• 1996

This study was done in order to investigate the protective effects of A.G.R.(Angelicae gigantis Radix) aqua-acupuncture on acetaminophen induced liver damage in rats. The liver damage was induced by acetaminophen (500mg/kg) injection into the peritoneum. The A.G.R. aqua-acupuncture solution was injected into the corresponding loci to Ganshu($BL_{18}$) and Qimen($LR_{14}$) of human body and a blank locus of the root of tail on four consecutive times at 0, 3, 6, and 12 hours after acetaminophen injection. And the serum GOT, GPT, LDH, ALP activities, total bilirubin, direct bilirubin levels were measured in the rats. The serum GOT, GPT, LDH ALP activities and bilirubin level were decreased comparing with that of a control group in case of A.G.R. aqua-acupuncture treated group, specially Ganshu and Qimen aqua-acupuncture treated groups showed an obvious significant decrease.

• 대한세포병리학회지
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• 제19권2호
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• pp.126-135
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• 2008

Malignant mesothelioma (MM) is a highly lethal neoplasm arising in pleura and the peritoneum and a rapid and accurate diagnosis is crucial for treatment of the disease. However, the sensitivity of cytological analysis using pleural or ascitic fluid is relatively low, yielding an accurate diagnosis in only $32{\sim}79%$ of cases. We tested the diagnostic value of epigenetic alterations in body fluid cytology as a supplement to conventional methods. Paraffin-embedded tissue blocks from 21 MM patients and associated body fluid cytology slides considered no evidence of malignancy were used to test for epigenetic alteration. Using methylation-specific PCR, we detected methylation of RASSF1A and p16 in 47.6% (10/21) of both surgically resected tumor samples, respectively. Body fluid samples of MM also showed abnormal methylation of RASSF1A and p16INK4a genes in 38.1% (8/21) and 33.3% (7/21) of cases. The concordance in the rates of RASSF1A and p16INK4a gene-methylation abnormalities determined from cytology samples and tissue samples were 61.9% (13/21) and 66.7% (14/21), respectively. Combining both genes increases the sensitivity of the test to 57.1 % (12 of 21) of cases. Our results suggest that testing for methylation abnormalities in selected individual genes or gene combinations has diagnostic value as an alternative or adjunct method to conventional cytological diagnosis.

• 한국발생생물학회지:발생과생식
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• 제10권3호
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• pp.193-196
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• 2006

이 연구는 태생경골어인 쏨뱅이의 번식생태학적 기초자료를 제공하고자 체내 자어와 출산 후의 시원생식세포의 출현, 원시 생식소와 난소 분화과정을 조직학적으로 조사하였다. 약 $10\;{\mu}m$의 시원생식 세포는 체내 자어의 초기 소화관과 중심신관 사이 복막에 위치하였다. 출산 후 31일째부터 생식원세포가 생식원기에 도달하였으며, 체세포들이 분열 증식하여 원시생식소를 형성하였고, 출산 후 49일째에 난소강이 형성되기 시작하였으며, 출산 후 60일째에 생식원기의 양쪽 끝에서부터 난소강 형성이 관찰되었다. 출산 후 79일째에는 난소강 안쪽을 따라 생식세포들이 분열 증식하기 시작하였다.

• 한국동물위생학회지
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• 제43권2호
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• pp.99-105
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• 2020

This case describes outbreaks of acute aspergillosis in a red-crowned crane. A six-month-old, male, crane had showed clinical signs (i.e. anorexia, performance loss, ruffled feathers and drooped wings and open mouth breathing, etc.) before death. In necropsy examination, spherical to oval nodules disseminated from the respiratory tract to other organs. Those nodules were formed predominantly in air sacs, lung, peritoneum, serosa of esophagus and trachea. The nodules varied in size from 1 mm to over 1cm and the color was white to yellow. Microscopically, most of lung architecture were replaced by multiple foci which were characterized by well demarcated eosinophilic and karyorrhetic debris and surrounded by numerous Inflammatory cell. Most within necrotic center of the nodules, large numbers of fungal hyphae were present. Microbiology result indicated fungal growths on sabroud dextrose agar and bacterial growths on blood agar. Bacteria identified as E. rhusiopathiae using MALDI-TOF (microflex, BRUKER, USA) and fungi identified as A. fumigatus, A. terreus by sequencing the ITS1 and ITS4 regions. To confirm the route of infection, we checked the existence of the same pathogens in cohabitant (i.e. mother crane). The young age and weakened immunity (i.e. bacterial infection, etc.) causes fatal aspergillosis in birds.

• Clinical and Experimental Reproductive Medicine
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• 제48권4호
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• pp.337-346
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• 2021

Objective: Red ginseng (RG) exerts anti-inflammatory, anti-proliferative, and immunomodulatory effects on endometriosis through the regulation of microRNA (miRNA) expression. It may also ameliorate endometriosis by affecting the expression of multiple miRNAs simultaneously, rather than acting on a single miRNA at a given time. Since studies on the overall effects of RG on endometriosis via the regulation of miRNA expression are lacking, the current study aimed to explore the global effect of RG on miRNA expression in a mouse model of endometriosis. Methods: To establish the mouse model, the uterine horn of donor mice was implanted into the lateral side of the recipients' peritoneum, followed by vehicle or RG treatment for 8 weeks. Results: To confirm the effects of RG on the established mouse model, the size of the implanted uterus was measured; it was found to be lower in mice from the RG group than in mice from the control group. miRNA expression profiles in the implanted uterus of the mouse model of endometriosis after vehicle or RG administration were analyzed using microarray technology. Thereafter, seven candidate miRNAs and 125 candidate genes (miRNA targets) were identified through a bioinformatics analysis. Conclusion: The present findings suggest that RG regulates the expression of multiple miRNAs and mRNAs, thereby alleviating endometriosis in a mouse model of the disease.

• 대한한방내과학회지
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• 제41권6호
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• pp.1274-1281
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• 2020

Objective: This case report shows symptom improvements in stage IV metastatic colorectal cancer treated with a Korean medicine-based integrative cancer treatment (ICT). Methods: A 61-year-old male patient diagnosed with colorectal cancer in November 2017 and metastasis to the lung, peritoneum, and liver in September 2020 was treated with Integrative Cancer Treatment (ICT) for abdominal pain, abdominal discomfort, and anorexia for 1 month. Clinical outcomes were measured with the National Cancer Institute Common Terminology Criteria for Adverse Event (NCI-CTCAE), a numeral rating (NRS), the Eastern Cooperative Oncology Group (ECOG), and the Functional Assessment of Cancer Therapy-General (FACT-G) scales. Results: After treatment, the patient's abdominal pain was improved from NRS 8 to 1, and the ECOG score of the patient was improved from grade 3 to 2. Integrative Cancer Treatment. Also the score improved on the FACT-G test, and there were no serious side effects of grade 3 or higher according to the NCI-CTCAE. Conclusion: This case study suggests that Korean medicine-based ICT may help to improve abdominal pain and quality of life in patients with metastatic colorectal cancer.

• Journal of Digestive Cancer Research
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• 제5권2호
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• pp.91-96
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• 2017

Background: To evaluate whether DM affects the SUVmax of metastatic lesions on ¹⁸F-FDG PET/CT and whether the SUVmax can influence the prognosis of metastatic PDAC patients. Methods: We conducted a retrospective study of 86 patients with metastatic PDAC who underwent PET/CT before treatment. The SUVmax of primary and metastatic lesions and the ratios of the SUVmax were measured. Long-term survival was evaluated using clinical parameters. Results: The mean SUVmax of primary lesion was lower in the DM group than in the non-DM group (4.74 vs. 5.96, p=0.009). The SUVmax for all metastatic lesions, except those in the lung, were lower in the DM group than in the non-DM group, and these differences were statistically significant in the lymph nodes and peritoneum. In the 35 patients with hepatic metastasis, higher ratios of the liver SUVmax significantly correlated with shorter OS (HR, 2.625; p=0.013). Conclusion: DM can influence the lower SUVmax of metastatic lesions as well as primary lesions. The SUVmax ratio of hepatic metastasis could influence on prognosis in metastatic PDAC patients.

• 대한영상의학회지
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• 제82권5호
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• pp.1304-1309
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• 2021

미분화성 다형성 육종은 중간엽조직에서 기원하는 고등급의 연조직 육종이다. 소장에서 기원한 미분화성 다형성 육종은 매우 드물며 산발적인 증례 보고가 대부분이다. 소장 미분화성 다형성 육종의 증상은 대개는 비특이적이며, 일부에서는 장중첩증으로 발현되어 간헐적 복통을 일으키기도 한다. 미분화성 다형성 육종은 공격적인 성장을 보이는 종양으로 진단 당시에 원격 전이를 동반한 경우가 많다. 가장 흔한 원격 전이 부위는 복막이며, 그 외에도 임파선, 복강 내 고형 장기, 폐나 뇌 등으로 전이할 수 있다. 저자들이 아는 한도에서는, 기관지 내 전이는 현재까지 보고된 바 없다. 저자들은 장중첩증으로 발현한 소장의 미분화성 다형성 육종이 기관지 내로 전이한 드문 증례를 경험하였기에 이를 보고하고자 한다.

• 대한영상의학회지
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• 제84권4호
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• pp.946-951
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• 2023

평활근종은 평활근세포에서 발생할 수 있는 흔한 양성종양으로, 주로 자궁에서 발생한다. 복막평활근종은 매우 드물며 대부분 복막성 평활근증으로 보고되었다. 저자들이 아는한 단일 복막평활근종의 영상의학적 소견은 영문으로 아직까지 보고된 바가 없다. 전산화단층촬영에서 비균질한 조영증강, 자기공명영상에서 점진적 조영증강 및 확산제한을 보여 영상의학적으로 간의 변성된 혈관종, 간선종, 혹은 염증성 근섬유아세포종 등을 감별하였으나 수술 후 병리학적으로 복막평활근종으로 진단된 34세 여성의 증례 및 영상의학적 소견을 보고하고자 한다.

• 한국임상수의학회지
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• 제20권1호
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• pp.12-21
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• 2003

이 연구에서는 개의 소장에 세가지 장문합법이 유착에 어떠한 영향을 미치는 지 알아보았다. 스물 여섯 마리의 개를 무작위로 세 군으로 나누었다. Group I은 단순 연속봉합을 실시하였고, Group II은 단순 결절 접합봉합을 실시하였으며, Group III는 단층 연속 코넬 봉합을 실시하였다. 모든 실험견에 단단장문합술 후 대망막이식을 실시하고, 복막을 닫기 바로 전에 1% Sodium carboxymethylcellulose을 체중 kg 당 5ml되는 용량으로 복강안에 주입하였다. 수술후 14일째 되는날 실험견들의 수술후의 유착소견을 관찰하였다. 모든 개에서 장문합 부위와 대망막 이식 부위 사이의 유착이 발견되었으며, 장막끼리의 유착이 Group I에서 두 마리, Group II에서 세미리, Group III에서 세 마리가 관찰되었다. 모든 군의 평균 유착 점수는 2이하였다. 장문합부와 대망막사이의 유착길이는 Group I에서 13.13$\pm$4.97mm(mean$\pm$S.D.), Group II에서 17.29$\pm$4.68mm, Group III에서 14.64$\pm$3.80mm가 관찰되었다. 단순 연속 봉합법이 유착을 가장 적게 일으켰고, 단순 결절 접합 봉합법이 가장 유착을 많이 일으켰다. 그러나 유착정도에 있어서 세가지 봉합법의 유의성 있는 큰 차이는 나타나지 않았다. 수술후 합병증인 장중적이 한 마리에서만 나타났고, 수술후 생존하였다. 14일간 매일 체온, 활력, 식욕, 배변 및 배뇨를 관찰하였다. 모두 정상범위에 속했으며, 군간의 뚜렷한 차이는 없었다. 결론적으로 개에서 세가지 단단장문합 봉합법이 유착정도에 미치는 영향에서 군간 유의성 있는 차이가 관찰되지 않았지만, 단순 연속 봉합법이 유착을 가장 적게 일으키므로 단단장문합법시에 다른 두기지 봉합법보다 더 권장될 수 있을것이라 사료된다.