Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Administration of red ginseng regulates microRNA expression in a mouse model of endometriosis

  • Lee, Jae Hoon (Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine) ;
  • Park, Ji Hyun (Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine) ;
  • Won, Bo Hee (Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine) ;
  • Im, Wooseok (Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine) ;
  • Cho, SiHyun (Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine)
  • Received : 2021.01.27
  • Accepted : 2021.07.12
  • Published : 2021.12.31
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Abstract

Objective: Red ginseng (RG) exerts anti-inflammatory, anti-proliferative, and immunomodulatory effects on endometriosis through the regulation of microRNA (miRNA) expression. It may also ameliorate endometriosis by affecting the expression of multiple miRNAs simultaneously, rather than acting on a single miRNA at a given time. Since studies on the overall effects of RG on endometriosis via the regulation of miRNA expression are lacking, the current study aimed to explore the global effect of RG on miRNA expression in a mouse model of endometriosis. Methods: To establish the mouse model, the uterine horn of donor mice was implanted into the lateral side of the recipients' peritoneum, followed by vehicle or RG treatment for 8 weeks. Results: To confirm the effects of RG on the established mouse model, the size of the implanted uterus was measured; it was found to be lower in mice from the RG group than in mice from the control group. miRNA expression profiles in the implanted uterus of the mouse model of endometriosis after vehicle or RG administration were analyzed using microarray technology. Thereafter, seven candidate miRNAs and 125 candidate genes (miRNA targets) were identified through a bioinformatics analysis. Conclusion: The present findings suggest that RG regulates the expression of multiple miRNAs and mRNAs, thereby alleviating endometriosis in a mouse model of the disease.

Keywords

Acknowledgement

This study was supported by the Korean Ginseng Cooperation. And, this study was financially supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI18C2047).

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