• 제목/요약/키워드: Periodontium

검색결과 180건 처리시간 0.022초

사람 치주인대줄기세포의 교원질 형성에 대한 법랑기질 유도체의 영향 (The Effect of Enamel Matrix Derivatives on the Collagen Formation by Human Periodontal Ligament Stem Cells both in vitro and in vivo Analysis)

  • Cha, Jae-Kook;Oh, Sang-Yeob;Park, Jung-Chul;Kim, Dong-Jun;Park, So-Yon;Cho, Kyoo-Sung;Kim, Chang-Sung
    • 대한치과의사협회지
    • /
    • 제53권12호
    • /
    • pp.935-948
    • /
    • 2015
  • 목적: 법랑기질 유도체(EMD)가 사람 치주인대 줄기세포(hPDLSC)의 조직 형성능에 미치는 영향을 in vitro와 in vivo 분석 모델을 이용해 평가한다. 재료 및 방법: hPDLSC를 배양하여 운반체와 함께 면역 억제된 쥐 등에 이식하였다; (1)대조군: EMD 처치하지 않은 운반체에 심어진 hPDLSC군 ($EMD^-/hPDLSC^+$), (2)실험군: EMD 처치한 운반체에 심어진 hPDLSC군 ($EMD^+/hPDLSC^+$). 각 군당 5마리씩 시행하고 8주 후 희생하였다. 조직학적, 조직계측학적 분석을 통해 형성된 백악질의 면적과 백악세포의 수 그리고 샤피 섬유의 수를 계측하였으며 면역조직화학적 분석을 통해 백악질과 교원질 형성을 평가하였다. 또한 in vitro에서 hPDLSC의 수용성 교원질과 glycosaminoglycan 형성에 대한 EMD의 효과를 분석하였다. 결과: 조직학적 분석에서 교원질성 치주 인대 조직이 실험군에서 현저하게 많이 생성된 것을 관찰할 수 있었다. 형성된 백악질의 면적과 백악세포의 수는 군 간 차이가 없었으나, 새롭게 형성된 샤피 섬유의 수는 실험군에서 대조군보다 유의하게 많았다(p<0.05). 교원질 형성에 대한 면역조직 화학적 분석 결과, 실험군에서 I, III형 교원질과 hydroxyproline의 발현이 높았다. 또한 in vitro에서 hPDLSC에 의한 수용성 교원질과 glycosaminoglycan 형성이 EMD의 농도에 비례하여 증가하였다 (p<0.05). 결론: EMD는 hPDLSC에 의한 샤피 섬유 및 교원질 생성을 증가시키고, 이는 새로운 백악질의 기능적 부착과 치주조직 재생에 중요한 역할을 한다.

성견 열개형 결손부에 DFDB이식과 Dura Mater막의 효과 (The Effects of DFDB combined with Dura mater on the Periodontal Wound Healing of Dehiscence Defects in Dogs)

  • 최성호;김일영;김영희;서종진;정현철;조규성;채중규
    • Journal of Periodontal and Implant Science
    • /
    • 제28권2호
    • /
    • pp.205-221
    • /
    • 1998
  • The present study invetigates the effects of root planing only(control group), DFDBA alone(test group 1) and combined use of DFDB and Dura mater(test group 2) in dehiscence defects in dogs. The results of 8weeks post-surgery by histological comparison between the three groups are as follows. 1. The contol group showed minimum regeneration of new cementum and new bone with limited migration of epitheilal cells, and healed by connective tissue attachment. 2. The test group 1 showed minimum regeneration of new cementum and new bone with limited migration of epitheilal cells, and healed by connective tissue attachment. 3. The test group 2 showed significant amount of the new cementum and new bone. 4. Both control and test groups healed without any observable root resorption and ankylosis. The above the results suggest that the use of resorbable Dura mater only does not improve the regeneration of new bone and periodontal ligament due to difficulties of space making, but the combined use with DFDB may be more effective.

  • PDF

초기치료 시행 후 치아동요도와 교합력의 변화에 관한 연구 (A STUDY OF THE CHANGES OF THE TOOTH MOBILITY AND MAXIMAL BITE FORCE FOLLOWING INITIAL THERAPY)

  • 정효선;이만섭
    • Journal of Periodontal and Implant Science
    • /
    • 제23권3호
    • /
    • pp.526-534
    • /
    • 1993
  • The purpose of this study was to evaluate the changes of the tooth mobility and maximal bite force over 4 weeks following initial therapy on the periodontal disease. Tooth mobility and maximal bite force due to change of viscoelastic property of periodontium were influenced by inflammation of periodontal tissue. 10 patients with the chronic adult periodontitis participated in this study. Each tooth was divided into anterior areas, premolar areas and molar areas. Tooth mobility was tested using Periotest(Siemens Co. Germany) and maximal bite force was evaluated with MPM-3000(Nihon kohden Co. Japan). Tooth mobility and maximal bite force were recorded at the initial examination, 1, 2, 3 and 4 weeks following initial therapy. All data were analyzed statistically. The obtained results were as follows ; 1. The changes of the tooth mobility following initial therapy were generally decreased in maxilla, showing the significant decrease at 1 and 4 weeks on premolar areas (p<0. 05). 2. The changes of the tooth mobility following initial therapy were generally decreased in mandible, however this changes were not statistically significant. 3. The changes of the maximal bite force following initial therapy in maxilla were significantly increased at 3 and 4 weeks on anterior areas, at 4 weeks on premolar areas (p<0. 05). These were decreased at 1 week on molar areas, but generally increasing with time. 4. The changes of the maximal bite force following initial therapy in mandible were significantly increased at 3 and 4 weeks on anterior areas (p<0. 05, p<0. 01). These were decreased at 1 week on premolar but molar areas, and generally increasing with time. 5. As tooth mobility increased, maximal bite force decreased with significance (p<0. 01), and they had high negative correlation on anterior areas but low negative correlation on premolar and molar areas.

  • PDF

한국인의 상악 전치부 심미 인지 선호도 및 정상 치은의 형태 분포 (Perception of maxillary anterior esthetics by dental professionals and lay people and topographical tooth-gingiva interface)

  • 안기연;이주연;김성조;최점일
    • Journal of Periodontal and Implant Science
    • /
    • 제36권4호
    • /
    • pp.817-827
    • /
    • 2006
  • Purpose : This study was designed 1) to compare the perception of dental professionals and lay people with respect to minor variations in maxillary anterior tooth size and alignment and their relation to the surrounding soft tissues, and 2) to evaluate the normal tooth-gingiva topographical relationships in periodontally healthy young subjects, Materials and methods : Maxillary anterior teeth were intentionally diagrammed in varying degree of deviation with respect to one of three common anterior esthetic discrepancies including variations in crown length, shape of gingival margin, and length of interproximal contact, 17 images were generated to be preferentially selected by 2 groups consisting of dental professionals and lay people (total of 740). Smiling photographs of 120 dental students who had healthy periodontium were taken and the photographic images were analyzed to be classified as 17 kinds of altered image groups. Results : The results demonstrated noticeable difference between the varying levels of discrepancy. Both group preferred gingival margin of lateral incisor to be 0.5mm lower than that of central incisor. Lay people preferred the gingival margin shape that has 2/9 horizontal component of the crown width, while dental professionals preferred the gingival margin shape that has 1/9 horizontal component of the crown width. Lay people preferred longer length of the interproximal contact (two thirds of the crown length), whereas dental professionals preferred shorter length of the interproximal contact (half of the crown length). Photographic analysis of normal esthetic gingival topography revealed 2/9 horizontal component and short length of the interproximal contact which was of the hybrid nature of the preferences shared by lay people and dental professionals. Conclusion: The results of this study show that dental professionals and lay people demonstrated significant difference in their preference of dental esthetic components, which may then influence the decision making process by dental professionals with respect to designing the anterior esthetic gingival line.

중증 치주염에 의해 발거된 치아의 수평부착상실에 대한 연구 (Horizontal attachment loss in extracted teeth due to severe periodontitis)

  • 김진숙;김성조;최점일;이주연
    • Journal of Periodontal and Implant Science
    • /
    • 제38권1호
    • /
    • pp.15-22
    • /
    • 2008
  • Purpose: The attachment level is strongly associated with tooth loss and provides useful information on patterns of destruction of the periodontium. The presence of horizontal attachment loss would not be detected in clinical measurement. Therefore, the purpose of the present study was to estimate the patterns of periodontal destruction based on the attachment area and horizontal attachment loss in extracted teeth due to severe periodontitis. Materials and Methods: 307 teeth satisfied the criteria for assessment. An indirect method, based on digital images obtained from a digital camera and an image analysis program, was used to calculate the area of root surface and attachment loss and the extent of horizontal attachment loss. The data were analysed using SPSS. Results: No statistically significant differences among root surfaces were observed in anterior teeth on the loss of attachment area. However, in posterior teeth statistically significant differences in palatal surfaces of maxillary and mandibular premolar and molar surfaces compared with buccal surfaces were observed. Horizontal attachment loss was observed in 21.5% of the teeth examined. Frequency of horizontal attachment loss was highest in the maxillary first premolar (34.8%), followed by the maxillary second premolar (27.3%) and maxillary canine (25%). The mean length of horizontal attachment loss was 1.5mm. Conclusion: More meticulous examination will be needed of the palatal surfaces of maxillary and mandibular premolar and molar teeth. The percentage of teeth with horizontal attachment loss greater than 2.1 mm was 5.2%. Considering the length of curette blades, about 5.2% of teeth were not properly debrided. Therefore, Additional supportive therapy such as local drug delivery has to be considered in treatment of the first maxillary, second premolar and canine due to the high prevalence of horizontal attachment loss.

Comparisons of the diagnostic accuracies of optical coherence tomography, micro-computed tomography, and histology in periodontal disease: an ex vivo study

  • Park, Jin-Young;Chung, Jung-Ho;Lee, Jung-Seok;Kim, Hee-Jin;Choi, Seong-Ho;Jung, Ui-Won
    • Journal of Periodontal and Implant Science
    • /
    • 제47권1호
    • /
    • pp.30-40
    • /
    • 2017
  • Purpose: Optical coherence tomography (OCT) is a noninvasive diagnostic technique that may be useful for both qualitative and quantitative analyses of the periodontium. Micro-computed tomography (micro-CT) is another noninvasive imaging technique capable of providing submicron spatial resolution. The purpose of this study was to present periodontal images obtained using ex vivo dental OCT and to compare OCT images with micro-CT images and histologic sections. Methods: Images of ex vivo canine periodontal structures were obtained using OCT. Biologic depth measurements made using OCT were compared to measurements made on histologic sections prepared from the same sites. Visual comparisons were made among OCT, micro-CT, and histologic sections to evaluate whether anatomical details were accurately revealed by OCT. Results: The periodontal tissue contour, gingival sulcus, and the presence of supragingival and subgingival calculus could be visualized using OCT. OCT was able to depict the surface topography of the dentogingival complex with higher resolution than micro-CT, but the imaging depth was typically limited to 1.2-1.5 mm. Biologic depth measurements made using OCT were a mean of 0.51 mm shallower than the histologic measurements. Conclusions: Dental OCT as used in this study was able to generate high-resolution, cross-sectional images of the superficial portions of periodontal structures. Improvements in imaging depth and the development of an intraoral sensor are likely to make OCT a useful technique for periodontal applications.

상악 유중절치의 병적 치근 흡수 (PATHOLOGIC ROOT RESORPTION OF PRIMARY CENTRAL INCISORS)

  • 최병재;정주현;최형준;손흥규
    • 대한소아치과학회지
    • /
    • 제30권4호
    • /
    • pp.605-610
    • /
    • 2003
  • 계승 영구치의 맹출시 상방에 위치한 유치의 치에서는 영구치와 근접한 치근첨에서부터 치근의 흡수가 일어나며 이를 생리적 치근 흡수라고 한다. 정상적인 영구치의 맹출과 관계없이 외상, 재식술, 교정치료, 치아의 지연 맹출, 불규칙적 맹출, 또는 종양이나 낭종의 성장 등과 관련되어 나타나는 치근의 흡수를 병적 치근 흡수라고 하며 생리적 치근 흡수와는 그 양상이 상이하고 다양하게 나타난다. 본 증례에서는 외상을 주소로 내원한 어린이를 대상으로 치근단 방사선 사진상에 나타나는 다양한 상악 유중절치의 병적 치근 흡수 양상을 관찰하여 다음과 같은 결과를 얻었다. 1. 외상성 손상을 받은 10개의 상악 유중절치에서 여러 가지 병적 치근 흡수 양상을 관찰하였다. 2. 병적 치근 흡수된 치아에서 치수절제술을 시행한 후에도 흡수는 지속적으로 진행되었다.

  • PDF

백서 치주인대세포의 분화에 대한 Bone morphogenetic protein-7의 영향 (Effect of BMP-7 on osteoblastic differentiation of rat periodontal ligament cells)

  • 이호재;김영준;정현주
    • Journal of Periodontal and Implant Science
    • /
    • 제35권3호
    • /
    • pp.747-760
    • /
    • 2005
  • Periodontal therapy has dealt primarily with attempts at arresting progression of disease. however, more recent techniques have focused on regenerating the periodontal ligament having the capacity to regenerate the periodontium. Recombinant human bone morphogenetic protein-7(rhBMP-7) can differentiate the osteoprogenitor cells and induce bone formation. The purpose of this study was to evaluate the effect of BMP-7 on rat periodontal ligament cells differentiation, in vitro. In the control group, cells was cultured with DMEM media. In the experimental groups, cells were cultured with rhBMP-7 in concentration of 10, 25, 50 and 100 ng/ml. Each group was characterized by examining alkaline phosphatase activity at 3 and 5 days of culture and the ability to produce mineralized nodules of rat calvarial cells at 14 days of culture. Synthesis of type I collagen(COL-I), osteocalcin(OCN), and bone sialoprotein(BSP) was evaluated by RT-PCR at 7 days of culture. Activation of Smad proteins and p38 MAP kinase was determined by western blot analysis of the cell lysates. Alkaline phosphatase activity was significantly increased in the concentration of BMP-7 50 ng/ml and 100 ng/ml compared to the control(p<0.05). The mineralized bone nodule formation was greater with addition of 50 ng/ml and 100 ng/ml BMP-7 than the control(p<0.01). In 7 days' culture, the expressions of COL-I, BSP, and OCN was increased by BMP-7 in concentration of 10 $ng/ml{\sim}100$ ng/ml. In western blot analysis, BMP-7 treated culture cells expressed Smad 1,5,8 in dose-dependent manner, whereas BMP-7 did not activate phosphorylated form of p38 MAP kinase. These result suggested that BMP-7 stimulate rat periodontal ligament cells to differentiate toward osteoblast phenotype and increase bone matrix production by activation of BMP-Smad pathway.

Comparison of pain intensity of anterior middle superior alveolar injection with infiltration anesthetic technique in maxillary periodontal surge

  • Shirmohammadi, Adileh;Faramarzi, Masoumeh;Lafzi, Ardeshir;Kashefimehr, Atabak;Malek, Sepideh
    • Journal of Periodontal and Implant Science
    • /
    • 제42권2호
    • /
    • pp.45-49
    • /
    • 2012
  • Purpose: The aim of the present clinical trial was to compare pain during injection of anterior middle superior alveolar (AMSA) technique with that of infiltration injection technique in the maxilla in periodontal flap surgeries of patients referring to the Department of Periodontics, Faculty of Dentistry, Tabriz University of Medical Sciences. Methods: Twenty subjects with an age range of 20 to 40 years were selected for the present study. One side of the maxilla was randomly selected as the test side and the other as the control side using a flip of a coin. AMSA technique was used on the test side and infiltration technique was used on the control side for anesthesia. On both sides 2% lidocaine containing 1:80,000 epinephrine was used for anesthesia. The operator obtained the visual analogue scale for each patient immediately after the injection and immediately after surgery. Data was analyzed using descriptive statistical methods (frequency percentages, means and standard deviations) and Wilcoxon's test using SPSS ver. 13 (SPSS Inc.). Statistical significance was defined at P<0.05. Results: There were no statistically significant differences in pain during injection between the two techniques (P=0.856). There were statistically significant differences in postoperative pain between the two injection techniques (P=0.024). Conclusions: Postoperative pain in AMSA injection technique was less than that in the infiltration technique. Therefore, the AMSA technique is preferable in the periodontal surgeries for the anesthesia of palatal tissues given the fact that it has other advantages, too.

수종의 클래스프의 언더커트에 따른 인장력에 관한 연구 (A STUDY OF THE TENSILE LOAD OF SEVERAL CLASPS ACCORDING TO VARIOUS UNDERCUT AREA)

  • 김붕환;임주환;조인호
    • 대한치과보철학회지
    • /
    • 제35권3호
    • /
    • pp.470-485
    • /
    • 1997
  • A fundamental principle in clasp selection for a specific abutment is the reduction of the transmission of excessive forces to the abutment tooth. Those forces include tilting, tipping, and stress on the abutment tooth. The flexibility of a clasp was believed to directly affect the reduction of such forces. Opinions have been expressed concerning the proper type of clasp to be used to prevent stress on periodontium. In order to evaluate and compare the various designs of a clasp system, it is necessary to measure these forces. This study compared the average measurements of forces required to dislodge three kinds of circumferential clasps having different amount of undercuts : the first with a round retentive arm, the second with a half round retentive arm, the third with a wrought wire retentive arm under tensile load. Three commonly used undercuts( 0.01, 0.02, 0.03 inch) were created on nine cast crowns, premolars and molars. The test was run six times for a same clasp. The means of tensile load required to dislodge each of the different clasps were compared statistically using the ANOVA test and multiple range test (Duncan test). The results were as follows. 1. The amount of tensile load of the wrought wire clasp was significantly different from the cast round or half round clasp (p<0.05). 2. The more amount of the undercut, the more tensile load was needed to dislodge the clasps. There were significant differences among them (p<0.05). 3. The molar showed higher tensile load than the premolar, and there was significant difference (p<0.05). 4. The means of tensile load according to clasp types showed significant differences at the molar between wrought wire clasp and cast clasp (p<0.05), but did not at the premolar.

  • PDF