• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.170-171
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• 2006

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.08a
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• pp.200-201
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• 2005

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.189-189
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• 2005

• Journal of Environmental Science International
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• v.19 no.10
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• pp.1301-1305
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• 2010

This study was carried out to investigate the effect of KEM and SAF appication on contents change of fatty acids and organic acid of perilla(Perilla Frutescens Britton). Contenst of squalene in perilla leaves on control and KEM/SAF treated were 3.39 mg and 4.22 mg, respectively. Therefore the squalene quantity of KEM/SAF treated leaves was 24.2% more than that of control. A total 6 fatty acids in perilla leaves were analyzed in this study. Percentage of the saturated and unsaturated fatty acid in perilla leaves were 20 and 80%, respectively. Contents of phytosterols in perilla leaf such as campesterol and sitosterol were 2.0 and 20.0 mg, respectively. Therefore sitosterol content was 10 fold more than that of campesterol. The KEM/SAF application on perilla leaf was effective on the change of squalene or phytosterol contents. However effect of that was negligible on the change of fatty acid content.

• Korean Journal of Agricultural Science
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• v.49 no.2
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• pp.357-366
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• 2022

Biochar can be obtained by using various types of biomass under an oxygen-limited condition. Biochar can be utilized for various applications such as soil improvement, waste management, growth promotion, and adsorption. Wood vinegar is produced by the process of pyrolysis wood biomass and is used as a growth promoter, for soil improvement, and as a feed additive. When wood vinegar is treated on soil, it acts to control soil pH, improve nutrient availability, and alleviate N₂O and NH₃ volatilization. The objective of this study was to evaluate the effect of biochar and wood vinegar on the growth of perilla and soil quality. The experiment was conducted by using a Wagner pot (1·5,000 a^-1) in a glass greenhouse. The biochar was produced by pyrolysis at 450℃ for 30 minutes using rice husk and rice straw. Wood vinegar was diluted to 1 : 500 (v·v^-1) and used in this experiement. In the results of a cultivation experiment, co-application of biochar and wood vinegar enhanced the growth of perilla. In particular, rice husk biochar affected the leaves of the perilla, and rice straw biochar influenced the stems of the perilla. In addition, soil quality after treatment with biochar and wood vinegar applied together was highest compared to other units. Therefore, it is anticipated that co-application of biochar and wood vinegar will be more productive and improve soil quality compared to individual utilization of biochar and wood vinegar.

• Biomolecules & Therapeutics
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• v.24 no.3
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• pp.338-345
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• 2016

Neurodegenerative diseases are often associated with oxidative damage in neuronal cells. This study was conducted to investigate the neuro-protective effect of methanolic (MeOH) extract of Perilla frutescens var. japonica and its one of the major compounds, rosmarinic acid, under oxidative stress induced by hydrogen peroxide ($H_2O_2$) in C6 glial cells. Exposure of C6 glial cells to $H_2O_2$ enhanced oxidative damage as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and thiobarbituric acid-reactive substance assays. The MeOH extract and rosmarinic acid prevented oxidative stress by increasing cell viability and inhibiting cellular lipid peroxidation. In addition, the MeOH extract and rosmarinic acid reduced $H_2O_2-indcued$ expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the transcriptional level. Moreover, iNOS and COX-2 protein expression was down-regulated in $H_2O_2-indcued$ C6 glial cells treated with the MeOH extract and rosmarinic acid. These findings suggest that P. frutescens var. japonica and rosmarinic acid could prevent the progression of neurodegenerative diseases through attenuation of neuronal oxidative stress.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.4
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• pp.581-585
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• 2011

This study investigated the quality of muffin prepared with various concentrations of freeze dried-perilla leaves (Perilla frutescens var. japonica HARA) powder. Muffin was prepared by addition of 0, 3, 6 and 9% powder to the flour of basic formulation. The weight of muffin decreased with increase in freeze dried-perilla leaves powder concentration, but no constant tendency in the bottom and upper dimensions by its addition was found. The height and baking loss rate of the muffin increased with increase in the powder concentration but with no significant differences. Lightness and yellowness of muffin decreased as the concentration of the powder increased, whereas no significant differences in the redness was found with increased powder concentration. The hardness of muffin increased, and springiness decreased with increase in the powder concentration, although not significant. The cohesiveness, gumminess and chewiness showed no consistent tendency by addition of the powder. The antioxidative activity measured by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of muffin increased as the concentration of the powder increased. The muffin contained 0~6% freeze dried-perilla leaves powder had acceptable sensory properties, such as color, taste, and overall acceptability. Results indicated that optimal concentration of freeze dried-perilla leaves powder into the muffin formula was 3~6% (w/w).

• Journal of Life Science
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• v.8 no.3
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• pp.318-325
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• 1998

To investigate changes in protein and total lipid contents, seed storage protein pattern, and fatty acid composition of germination perilla(Perilla frutescens) seeds. Also, the corresponding value components in cotyledons, hypocotyles and roots were measured according to germination stage. The results were summarized as follows ; During germination, pertein and total lipid contents of Yepsilldalggae and Kwangyang cultivar were decreased continuously. In particular, protein contents rapidly decreased to the 3 days after germination(DAG), and then total lipid contents rapidly decreased between 3 DAG and 10 DAG. In changes of protein and total lipid contents of cotyledons, protein contents of Yeupsildalggae was increased during the germination, but Kwangyang cultivar was decreased during the same periods. The total lipids contents of Yeupsildalggae and Kwangyang cultivar were decreased during the germination. According to SDS-PAGE analysis, there was no detectible polypeptide bands on the gel before seed germination suggesting that this may be due to the rapid degradation of the storage proteins in the mature seed by hydrolyttic enzymes during the stage. During germinatation , the polypeptide band with 27$\sim$28KD of Yeupsildalggae and Kwangyang cultivar were accumulated gradually. In changes of fatty acid composition of total lipid of Yeupsildalggae and Kwangyang cultivar , saturated fatty acids such as palmitic acid and stearic acid increased during the germination. On the other hand, unsaturated fatty acid such as linoleic acid and linolenic acid decreased during the same periods. However, oleic acid increased to the 5 DAG, and then was repidly decreased.

• Korean Journal of Environmental Agriculture
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• v.7 no.1
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• pp.43-51
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• 1988

$SO_2$ gas injuries for 19 varieties of 10 species of plants were investigated within a environmentally controlled growth chamber. Visible injuries were observed when exposing the plants either 0, 0.2, 0.4, 0.7 or 1.5ppm of $SO_2$ gas for 5 days from 9 : 00 to 17 : 00 everyday. The results obtained were as follows: 1. Light brown spots were shown on Raphanus sativus(Chunchu, Taeback, Jinjudaepyung), Brassica campestris(Manchun, Josaengmiho, Seoul, Jeonseung), and Capsicum annum(Searona, Hongsanho, Hongilpum); brown spots on Sesamum indicum(Pungnyun, Kwangsan); reddish brown shades on Cleosia cristata; and black brown spots on Perilla frutescens, Calendula officinalis, Chrysanthemum morifolium, and Salvia splendens, respectively. 2. The spotty injuries induced by $SO_2$ gas injuries for 19 varieties of 10 species of plants were investigated within a environmentally controlled growth chamber. Visible injuries were observed when exposing the plants either 0, 0.2, 0.4, 0.7 or 1.5ppm of $SO_2$ gas for 5 days from 9 : 00 to 17 : 00 everyday. The results obtained were as follows: 1. Light brown spots were shown on Raphanus sativus(Chunchu, Taeback, Jinjudaepyung), Brassica campestris(Manchun, Josaengmiho, Seoul, Jeonseung), and Capsicum annum(Searona, Hongsanho, Hongilpum); brown spots on Sesamum indicum(Pungnyun, Kwangsan); reddish brown shades on Cleosia cristata; and black brown spots on Perilla frutescens, Calendula officinalis, Chrysanthemum morifolium, and Salvia splendens, respectively. 2. The spotty injuries induced by SO₂ gas on Raphanus sativus, Callistephus chinensis, Capsicum annum, Perilla frutescens, Calendula officinalis, Salvia splendens, and Sesamum indicum; the many small spots on Chrysanthemum morifolium; and the brown shade on Celosia cristata appeared on the upper surface of the intervein, on the leaf apex area and on the entire upper surface of leaves, respectively. 3. Visual injuries of Capsicum annum(Chunchu), Perilla frutescens, Sesamum indicum(Pungnyun, Kwangsan), and Salvia splendens were developed at 0.4ppm of SO₂ gas fumigation. Brassica campestris(Jeonseung), Capsicum annum(Searona, Hongilpum), and Cleosia cristata, however, showed the injury at 1.5ppm of $SO_2$ gas concentration. 4. Based on the tolerance grade(time when the 1st injury appeared), Raphanus sativus, Perilla frutescens, Sesamum indicum, and Salvia splendens were the most sensitive plants and Chrysanthemum morifolium, Callistephus chinensis, Cleosia cristata, and Calendula officinalis were the plants most tolerant of $SO_2$ gas. gas on Raphanus sativus, Callistephus chinensis, Capsicum annum, Perilla frutescens, Calendula officinalis, Salvia splendens, and Sesamum indicum; the many small spots on Chrysanthemum morifolium; and the brown shade on Celosia cristata appeared on the upper surface of the intervein, on the leaf apex area and on the entire upper surface of leaves, respectively. 3. Visual injuries of Capsicum annum(Chunchu), Perilla frutescens, Sesamum indicum(Pungnyun, Kwangsan), and Salvia splendens were developed at 0.4ppm of $SO_2$ gas injuries for 19 varieties of 10 species of plants were investigated within a environmentally controlled growth chamber. Visible injuries were observed when exposing the plants either 0, 0.2, 0.4, 0.7 or 1.5ppm of $SO_2$ gas for 5 days from 9 : 00 to 17 : 00 everyday. The results obtained were as follows: 1. Light brown spots were shown on Raphanus sativus(Chunchu, Taeback, Jinjudaepyung), Brassica campestris(Manchun, Josaengmiho, Seoul, Jeonseung), and Capsicum annum(Searona, Hongsanho, Hongilpum); brown spots on Sesamum indicum(Pungnyun, Kwangsan); reddish brown shades on Cleosia cristata; and black brown spots on Perilla frutescens, Calendula officinalis, Chrysanthemum morifolium, and Salvia splendens, respectively. 2. The spotty injuries induced by SO₂ gas on Raphanus sativus, Callistephus chinensis, Capsicum annum, Perilla frutescens, Calendula officinalis, Salvia splendens, and Sesamum indicum; the many small spots on Chrysanthemum morifolium; and the brown shade on Celosia cristata appeared on the upper surface of the intervein, on the leaf apex area and on the entire upper surface of leaves, respectively. 3. Visual injuries of Capsicum annum(Chunchu), Perilla frutescens, Sesamum indicum(Pungnyun, Kwangsan), and Salvia splendens were developed at 0.4ppm of SO₂ gas fumigation. Brassica campestris(Jeonseung), Capsicum annum(Searona, Hongilpum), and Cleosia cristata, however, showed the injury at 1.5ppm of $SO_2$ gas concentration. 4. Based on the tolerance grade(time when the 1st injury appeared), Raphanus sativus, Perilla frutescens, Sesamum indicum, and Salvia splendens were the most sensitive plants and Chrysanthemum morifolium, Callistephus chinensis, Cleosia cristata, and Calendula officinalis were the plants most tolerant of $SO_2$ gas. gas fumigation. Brassica campestris(Jeonseung), Capsicum annum(Searona, Hongilpum), and Cleosia cristata, however, showed the injury at 1.5ppm of $SO_2$ gas concentration. 4. Based on the tolerance grade(time when the 1st injury appeared), Raphanus sativus, Perilla frutescens, Sesamum indicum, and Salvia splendens were the most sensitive plants and Chrysanthemum morifolium, Callistephus chinensis, Cleosia cristata, and Calendula officinalis were the plants most tolerant of $SO_2$ gas.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.1
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• pp.61-79
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• 2009

This study is for incompatible element contents of Perilla frutescens and Sesamum indicum from the Keumsan: biotite granite, phyllite and shale areas. In the soils, high elements are shown in the granite and phyllite areas, and in the areas of the Perilla frutescens. Positive correlations are distinctive within the granite for the Perilla frutescens, but the shale for the Sesamum indicum. These relationships can be explained with relative propositions of minerals containing the incompatible element. In the plants, high elements are shown in the shale and the Sesamum indicum are high in the comparisons of the same soil types. The low parts are mainly high. Regardless of the soil types, the lower and upper parts, respectively, are high in the Y, Zr and Rb contents for the Perilla frutescens, but, Ta, Nb, Th and U contents for the Sesamum indicum. Positive correlations are distinctive within plants of the phyllite. Comparing with the soil types, all HFS and Cs contents of the LFS in the plants are low with differences of several to hundred times, but high in the Sr contents with differences of several times. In the comparisons between plants and soil types, Y, Zr, Hf, Ta, Nb, Rb, and Sr of the phyllite and Th, U, Ba and Cs of the shale for the Perilla frutescens as well as Y, Zr, Hf, Rb, Sr, Ba and Cs of the phyllite and Ta, Nb, Th and U of the shale for the Sesamum indicum are chemically similar to the soils. In the comparisons of the each parts for the plant types, differences with the soils are big in the granite.