Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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The Neuro-Protective Effect of the Methanolic Extract of Perilla frutescens var. japonica and Rosmarinic Acid against H2O2-Induced Oxidative Stress in C6 Glial Cells

  • Lee, Ah Young (Department of Food Science and Nutrition& Kimchi Research Institute, Pusan National University) ;
  • Wu, Ting Ting (Department of Food Science and Nutrition& Kimchi Research Institute, Pusan National University) ;
  • Hwang, Bo Ra (Department of Food Science and Nutrition& Kimchi Research Institute, Pusan National University) ;
  • Lee, Jaemin (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Myoung-Hee (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition& Kimchi Research Institute, Pusan National University)
  • Received : 2015.08.25
  • Accepted : 2016.02.19
  • Published : 2016.05.01
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Abstract

Neurodegenerative diseases are often associated with oxidative damage in neuronal cells. This study was conducted to investigate the neuro-protective effect of methanolic (MeOH) extract of Perilla frutescens var. japonica and its one of the major compounds, rosmarinic acid, under oxidative stress induced by hydrogen peroxide ($H_2O_2$) in C6 glial cells. Exposure of C6 glial cells to $H_2O_2$ enhanced oxidative damage as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and thiobarbituric acid-reactive substance assays. The MeOH extract and rosmarinic acid prevented oxidative stress by increasing cell viability and inhibiting cellular lipid peroxidation. In addition, the MeOH extract and rosmarinic acid reduced $H_2O_2-indcued$ expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the transcriptional level. Moreover, iNOS and COX-2 protein expression was down-regulated in $H_2O_2-indcued$ C6 glial cells treated with the MeOH extract and rosmarinic acid. These findings suggest that P. frutescens var. japonica and rosmarinic acid could prevent the progression of neurodegenerative diseases through attenuation of neuronal oxidative stress.

Keywords

References

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