• 한국응용과학기술학회지
• /
• 제35권1호
• /
• pp.12-19
• /
• 2018

자소엽(perilla frutescens)은 꿀풀과(Labiatae)에 속하며 널리 알려져 있는 약용 식물이다. 본 연구에서는 자소엽을 물, 열수, 초음파 추출 방법으로 추출하여 항산화 효과를 비교하고 가장 효과적인 추출방법을 제시하고자 한다. 물, 열수, 초음파 처리를 통해 각각의 자소엽 추출물을 제조 하였고, DPPH 라디칼 소거능 및 총 페놀 함량을 통해 항산화 효과를 검증하고, 인간 간세포인 HepG2에 대한 세포 독성효과와 hydrogen peroxide ($H_2O_2$)로 유도된 산화적 스트레스로부터 간세포 보호효과를 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay로 확인하였다. 자소엽 초음파 추출물은 $5000{\mu}g/mL$농도에서 69.07%의 DPPH 라디칼 소거능을 나타내며 물, 열수 추출물과 비교하여 우수한 항산화 효과를 나타내었다. 또한 총 페놀 함량 측정 결과 $51.60{\pm}1.06mg\;GAE/g$ extract 로서 물, 열수 추출물 보다 높은 총 페놀 함량을 확인하였다. 그러나 산화적 스트레스에 의한 세포 보호효과는 미비하였다. 본 연구를 통해 추출 방법에 따른 항산화 효과의 차이를 확인하였으며, 우수한 항산화 효과를 나타낸 자소엽 초음파 추출물을 이용하여 추가적인 연구가 필요 할 것으로 사료된다.

• 한국식품영양학회지
• /
• 제28권1호
• /
• pp.66-72
• /
• 2015

This study was conducted to investigate the effect of Perilla frutescens powder on physiological and sensory characteristics in macaron$\grave{e}$. The perilla powders were added to macaron$\grave{e}$ at a weight percentage of 0, 2.5 and 4%. Color values (L-value, redness and yellowness index), total phenolics, DPPH radical scavenging activity, textures, total sugar contents and sensory characteristics of macaron$\grave{e}$ made with varying levels of perilla powder were measured. In sensory evaluation, significant differences (p<0.05 and p<0.01) were shown in color, sweetness, nuttyflavor, texture and overall acceptability depending on the addition level of perilla powders.

• Journal of Microbiology
• /
• 제45권2호
• /
• pp.128-132
• /
• 2007

The red yeast Xanthophyllomyces dendrorhous (previously named Phaffia rhodozyma) produces astaxanthin pigment among many carotenoids. The mutant X. dendrorhous G276 was isolated by chemical mutagenesis. The mutant produced about 2.0 mg of carotenoid per g of yeast cell dry weight and 8.0 mg/L of carotenoid after 5 days batch culture with YM media; in comparison, the parent strain produced 0.66 mg/g of yeast cell dry weight and a carotenoid concentration of 4.5 mg/L. We characterized the utilization of carbon sources by the mutant strain and screened various edible plant extracts to enhance the carotenoid production. The addition of Perilla frutescens (final concentration, 5%) or Allium fistulosum extracts (final concentration, 1%) enhanced the pigment production to about 32 mg/L. In a batch fermentor, addition of Perilla frutescens extract reduced the cultivation time by two days compared to control (no extract), which usually required five-day incubation to fully produce astaxanthin. The results suggest that plant extracts such as Perilla frutescens can effectively enhance astaxanthin production.

• 한국환경복원기술학회지
• /
• 제7권3호
• /
• pp.73-77
• /
• 2004

차즈기의 관상가치 증진 및 광도에 따른 생육반응을 알아보기 위하여 실험을 실시한 결과 차광정도에 따라 생육상태가 매우 다르게 나타났다. 초장, 수관폭, 엽병장, 엽장, 엽폭, 줄기굵기, 건물중 등 대부분이 30% 차광 시에 높게 나타나 생육이 가장 좋았으며, 차광율이 높아질수록 모두 감소하였다. 엽록소함량도 30% 차광시에 가장 높았으며, 안토시아닌함량의 경우 70% 차광시에만 감소하였을 뿐 나머지 처리구에서는 큰 차이가 없었다. 또한 70% 차광 시에는 엽색이 암자적색에서 황록색으로 변하였고, 생육이 저조하였을 뿐만 아니라 식물체가 도복하여 차즈기 고유의 관상가치가 떨어지는 결과를 보였다.

• 한국환경과학회지
• /
• 제5권2호
• /
• pp.171-178
• /
• 1996

This experiment was performed with the purpose of finding out the effect of simulated acid rain at various pH levels on the morphology and enzyme of Perilla frutescens var. japonica hara. The pH levels of simulated acid rain ranged from pH 2.0 to pH 6.0. The experiment showed the anion concentrations in the order of $SO_4^{2-}$, Cl^-$, $NO_3^-$, and $F^-$, $SO_4^{-2}$ was found out to be the main factor which contributed to the rainwater acidification. A general decrease of growth in Perilla frutescens var. japonica hara growth was shown with the decreas of pH concentration. As acidity increases a definite reduction in the rates of germination, heigth of plant, malate dehydrogenase, and 6-phosphogluconate dehydrogenase was ovserved, but the density of spots on the leaf apex was increased.

• 한국자원식물학회지
• /
• 제8권3호
• /
• pp.335-339
• /
• 1995

최근 소비자들의 신선한 채소 요구에 따른 수요증가로 볼 때 어린순의 소비는 크게 늘어 날 것으로 보나 국내에서는 무순외에는 거의 개발이 안된 실정이다. 이에 본 연구에서는 약용식물중에서 어린순을 채소로 개발할 수 있는 것을 선발하고자 기초연구로써 수행한 결과는 다음과 같았다. 1. 실험에 사용된 12종의 식물중에서 가걱은 들깨, 메밀, 무등이 낮은 값을 보였다. 2. 발아율은 전체적으로 낮았으나 그중 무, 소엽, 메밀, 항금 및 들깨가 상대적으로 높은 발아율을 보였다. 3. 발아세는 발아율보다 낮으며 그중 소엽, 무, 메밀 및 황금이 상대적으로 높은 발아세를 보였다. 4. 식미특성중 외관상으로 무가 가장 높은 점수를 보였다. 5. 맛은 황금이 가장 높은 점수를 보였다. 6. 조직감은 메밀이 가장 높은 점수를 보였다. 7. 기호도는 소엽이 가장 높은 점수를 보였다.

• Genes and Genomics
• /
• 제40권12호
• /
• pp.1319-1329
• /
• 2018

SSRs were successfully isolated from the Perilla crop in our current study, and used to analyze Perilla accessions from East Asia. Analyses of the clear genetic diversity and relationship for Perilla crop still remain insufficient. In this study, 40 new simple sequence repeat (SSR) primer sets were developed from RNA sequences using transcriptome analysis. These new SSR markers were applied to analyze the diversity, relationships, and population structure among 35 accessions of the two cultivated types of Perilla crop and their weedy types. A total of 220 alleles were identified at all loci, with an average of 5.5 alleles per locus and a range between 2 and 10 alleles per locus. The MAF (major allele frequency) per locus varied from 0.229 to 0.943, with an average of 0.466. The average polymorphic information content (PIC) value was 0.603, ranging from 0.102 to 0.837. The genetic diversity (GD) ranged from 0.108 to 0.854, with an average of 0.654. Based on population structure analysis, all accessions were divided into three groups: Group I, Group II and the admixed group. This study demonstrated the utility of new SSR analysis for the study of genetic diversity and population structure among 35 Perilla accessions. The GD of each locus for accessions of cultivated var. frutescens, weedy var. frutescens, cultivated var. crispa, and weedy var. crispa were 0.415, 0.606, 0.308, and 0.480, respectively. Both weedy accessions exhibited higher GD and PIC values than their cultivated types in East Asia. The new SSR primers of Perilla species reported in this study may provide potential genetic markers for population genetics to enhance our understanding of the genetic diversity, genetic relationship and population structure of the cultivated and weedy types of P. frutescens in East Asia. In addition, new Perilla SSR primers developed from RNA-seq can be used in the future for cultivar identification, conservation of Perilla germplasm resources, genome mapping and tagging of important genes/QTLs for Perilla breeding programs.

• 한국자원식물학회지
• /
• 제25권6호
• /
• pp.762-772
• /
• 2012

The objective of this study was to analyze the genetic diversity using SSR marker and investigate the fatty acid composition of perilla (P. frutescens var. frutescens) germplasm. Genetic diversity among 95 accessions, which consisted of 29 weedy types and 66 landrace accessions, was evaluated based on 12 SSR markers carrying 91 alleles. The mean values of observed ($H_O$) and expected heterozygosities ($H_E$) were 0.574 and 0.640, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into two distinct groups, which were the landrace, moderate and weedy type, genetic distance (GD) value was 0.609. The physicochemical traits about crude oil contents and fatty acid compositions were analyzed using GC. Among tested germplasm, the total average oil contents (%) showed a range from 28.57 to 49.67 %. Five fatty acids and their contents in the crude oils are as follows: ${\alpha}$-linolenic acid (41.12%-51.81%), linoleic acid (15.38%-16.43%), oleic acid (18.93%-27.28%), stearic acid (2.56%-4.01%), and palmitic acid (7.38%-10.77%). The average oil content of wild types was lower than landrace, and the oil content of middle genotype accessions was higher than other germplasm, but no significant variation between landrace and wild types was shown. Nevertheless, IT117174, landrace of Korea, was highest in crude oil content (47.11%) and linolenic acid composition (64.58%) among the used germplasm. These traits of the selected accessions will be helped for new functional plant breeding in perilla crop.

• 한국식품저장유통학회지
• /
• 제6권2호
• /
• pp.239-244
• /
• 1999

자소잎중 물과 에탄올을 이용하여 얻은 추출물을 2종의 부패곰팡이(Aspergillus flavus KCTC 6143, Aspergillus jlavus KcrC 6961) 및 5종의 식중독세균 ( isteria monocytogenes ATCC 15313, Staphylococc따 aureus 196E ATCC 13565, Escherichia coli 0157:H7 ATCC 43895, Salmonella typhimurium ATCC 13311, Yersinia enterocolitica)에 대하여 항균효과를 조사하였다. 생육증식 저해농도를 disk method로 검색한 결과 에탄올추출물에서 그 효과가 인정되어 5종의 식중독 세균에 대하여 자소잎 에탄올 추출물을 농도별로 첨 가한 후 그 효과를 비교하였다. Gram양성균(L. monocytogenes, S. aureus)의 경우가 Gram음성균(E. coli 0157:H7, S. typhimurium, Y. enterocolitica)에 비하 여자소잎 에탄올추출물에 의한 증식억제효과가 추 출물 $1000\mu\textrm{g}$/mL 농도 첨가구에서 월둥히 컸으며 생 육증식억제효과의 크기는 L. monocytogenes, S. aureus, S. typhimurium의 순이었고, E. coli 0157:H7과 Y enterocolitica균에 대한 생육증식억제 효과는 농도첨 가별에 따라 생육증식이 비례적으로 억제되었다. 자 소잎 에탄올 추출물은 $121^{\circ}C$에서 15분간 가열처리한 후에도 항균력이 인정되었다.

• Natural Product Sciences
• /
• 제22권4호
• /
• pp.275-281
• /
• 2016

Perilla frutescens was empirically used for controlling airway inflammatory diseases in folk medicine. We investigated whether caffeic acid, myristicin and rosemarinic acid derived from Perilla frutescens significantly affect the gene expression and production of mucin from airway epithelial cells. Confluent NCI-H292 cells were pretreated with caffeic acid, myristicin or rosemarinic acid for 30 min and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h. The MUC5AC mucin gene expression and production were measured by reverse transcription - polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Additionally, we examined whether caffeic acid, myristicin or rosemarinic acid affects MUC5AC mucin production indued by epidermal growth factor (EGF) and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), the other two stimulators of production of airway mucin. The results were as follows: (1) Caffeic acid, myristicin and rosemarinic acid inhibited the gene expression and production of MUC5AC mucin induced by PMA from NCI-H292 cells, respectively; (2) Among the three compounds derived from Perilla frutescens, only rosemarinic acid inhibited the production of MUC5AC mucin induced by EGF or $TNF-{\alpha}$, the other two stimulators of production of airway mucin. These results suggest that rosemarinic acid derived from Perilla frutescens can regulate the production and gene expression of mucin, by directly acting on airway epithelial cells and, at least in part, explains the traditional use of Perilla frutescens as remedies for diverse inflammatory pulmonary diseases.