Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Comparison of Anti-Oxidative Activities of Perilla frutescens Extracts by Extraction Methods

추출 방법에 따른 자소엽 추출물의 항산화 효과 비교

  • Seo, In-Yeong (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Kim, Hee-Su (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Jang, Kyeong-Su (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Yeo, Min-Ho (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Kim, Hye-Ran (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Jung, Bo-Kyoung (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Chang, Kyung-Soo (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
  • 서인영 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 김희수 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 장경수 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 여민호 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 김혜란 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 정보경 (부산가톨릭대학교 보건과학대학 임상병리학과) ;
  • 장경수 (부산가톨릭대학교 보건과학대학 임상병리학과)
  • Received : 2018.01.15
  • Accepted : 2018.02.25
  • Published : 2018.03.30
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Abstract

Perilla frutescens (P. frutescens) is one of evergreen shrubs belonging to the Labiatae and is grown wildly in Korea. This study was carried out to evaluate the anti-oxidative effects of Perilla frutescens Extracts by Extraction Methods (water, heating and sonication). Anti-oxidative effects were measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and total phenol content. Cell viability and hepatoprotective effects were identified by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Among various extracts, P. rutescens extracts by sonication showed the highest DPPH radical scavenging activity at $5000{\mu}g/mL$. Total phenolic content in P. frutescens extracts by sonication was $51.60{\pm}1.06mg\;GAE/g$ extract. However, P. frutescens extracts did not show hepatoprotective effects. This study identified anti-oxidative effects of P. frutescens extracts by sonication, and it would be necessary to perform further studies of P. frutescens extracts by sonication.

자소엽(perilla frutescens)은 꿀풀과(Labiatae)에 속하며 널리 알려져 있는 약용 식물이다. 본 연구에서는 자소엽을 물, 열수, 초음파 추출 방법으로 추출하여 항산화 효과를 비교하고 가장 효과적인 추출방법을 제시하고자 한다. 물, 열수, 초음파 처리를 통해 각각의 자소엽 추출물을 제조 하였고, DPPH 라디칼 소거능 및 총 페놀 함량을 통해 항산화 효과를 검증하고, 인간 간세포인 HepG2에 대한 세포 독성효과와 hydrogen peroxide ($H_2O_2$)로 유도된 산화적 스트레스로부터 간세포 보호효과를 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay로 확인하였다. 자소엽 초음파 추출물은 $5000{\mu}g/mL$농도에서 69.07%의 DPPH 라디칼 소거능을 나타내며 물, 열수 추출물과 비교하여 우수한 항산화 효과를 나타내었다. 또한 총 페놀 함량 측정 결과 $51.60{\pm}1.06mg\;GAE/g$ extract 로서 물, 열수 추출물 보다 높은 총 페놀 함량을 확인하였다. 그러나 산화적 스트레스에 의한 세포 보호효과는 미비하였다. 본 연구를 통해 추출 방법에 따른 항산화 효과의 차이를 확인하였으며, 우수한 항산화 효과를 나타낸 자소엽 초음파 추출물을 이용하여 추가적인 연구가 필요 할 것으로 사료된다.

Keywords

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