• Journal of Microbiology
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• v.45 no.3
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• pp.272-274
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• 2007

During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type $metallo-{\beta}-lactamase$ gene and PER-1 type extended spectrum ${\beta}-lactamase$ gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying $bla_{VIM-2},\;bla_{PER-1}$, and armA genes.

• Molecules and Cells
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• v.22 no.3
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• pp.285-290
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• 2006

The light sensing system in the eye directly affects the circadian oscillator in the mammalian suprachiasmatic nucleus (SCN). To investigate this relationship in the rat, we examined the circadian expression of clock genes in the SCN and eye tissue during a 24 h day/night cycle. In the SCN, rPer1 and rPer2 mRNAs were expressed in a clear circadian rhythm like rCry1 and rCry2 mRNAs, whereas the level of BMAL1 and CLOCK mRNAs decreased during the day and increased during the night with a relatively low amplitude. It seems that the clock genes of the SCN may function in response to a master clock oscillation in the rat. In the eye, the rCry1 and rCry2 were expressed in a circadian rhythm with an increase during subjective day and a decrease during subjective night. However, the expression of Opn4 mRNA did not exhibit a clear circadian pattern, although its expression was higher in daytime than at night. This suggests that cryptochromes located in the eye, rather than melanopsin, are the major photoreceptive system for synchronizing the circadian rhythm of the SCN in the rat.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.1
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• pp.453-461
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• 2015

The aim of this study was to find out the relationship between hospital structural factors and nurses' turnover rate. Nurse turnover rate and the hospital's characteristics was using Statistics for Hospital Management Data of Korean Health Industry Development Institute. The data were reported 247 hospitals in 2008 all over the country. The turnover rate among nurses was 32.0% in Korea as of 2008; those factors actually affecting the rate included whether the hospital was managed publicly or privately, the hospital size, region, the average salary for nurses, and the number of nurses per 100 beds. While each hospital had the turnover rate correlated with the total number of beds (r=-.322), the average salary for nurses(r=-.186) and the number of nurses per 100 beds (r=-.390), variables explaining the turnover rate were whether the hospital was managed publicly or privately, the number of beds, and the number of nurses per 100 beds ($R^2=.257$). In conclusion, nurses working in a privately-managed hospital and hospitals which had a smaller number of beds and which had a smaller number of nurses per 100 beds showed a higher turnover rate. Further study is required to elucidate the factors of the nurses' turnover.

• Development and Reproduction
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• v.8 no.1
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• pp.57-64
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• 2004

This study was carried out to examine the expression of the circadian clock genes in the mouse ovary and testis at different developmental stages. Expression of Period1(Per 1), Period2(Per2), Period3(Per3), Cryptochrome1(Cry1), Cyptochrome2(Cry2), Clock Small and Prokineticin1 and Prokineticin2 receptor(Prok1r, Prok2r) genes in mouse ovary was explored by semiquantitative reverse transcription Polymerase chain reaction(RT-PCR) according to the developmental stage(post partum day; ppd 1, 7, 10, 21 and 35). Immunohistochemistry using PER1 antibody was also analyzed. The differential expression pattern of clock genes was presented according to stages of the mouse ovarian development (ppd 1, 7, 10, 21 and 35). In the cases of ovaries, at the starting point of follicle growth at ppd 7 and 10, the clock gene expression patterns were changed vastly. According to the developmental stages, the clock genes were highly expressed at ppd 7 and 10 in mouse testis also. Receptors for Prok2, the circadian output molecule of SCN, were also expressed in ovary at ppd 7 and in testis at ppd 1 and 7, respectively. Immnunohistochemical analysis of PER1 showed positive signals in the cytoplasm of oocytes and granulosa cells. The level or PER1 expression was increased in cells at the spermatogonia and the condensing spermatids. The expression pattern of Perl and localization of PER1 were showed similar patterns according to the developmental stages in ovary and testis. Taken together, it could be observed that the expression of clock genes was highly correlated with gonadal development and germ cell differentiation in mice. Therefore, in this study, circadian programming of the genes in the ovary and testis is strongly imposed across a wide range of core reproductive cycles and normal development of gametes. Although the existence of circadian genes is clearly investigated, further studies on the direct evidence is required for the understanding of the relationship between circadian genes and regulation of gonadal differentiation and germ cell development.

• 한국가스학회:학술대회논문집
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• 2005.10a
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• pp.141-145
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• 2005

DME (Di-Methyl Ether) is a new clean fuel and an environmental-friendly energy resource, also is recently increasing with an alternative interest because of the industrial use. DME has been shown to have excellent properties as a diesel fuel giving emission level better than ULEV standard. So it has been attracting considerable as an alternative diesel fuel. In this study, we carried out simulation of separation and purification process of demo plant for 101on per day DME production, which cause the effect that is important in productivity, from operation results of pilot plant for 50kg per day DME production. The liquefied stream, which was separated by gas-liquid separator after DME reactor, includes $CO_2$, DME, Methanol and $H_2O$. We established three distillation columns for separation and purification of the stream. $CO_2$ was extracted from the stream by first distillation column, DME was extracted by second column and Methanol was extracted by third column. We investigated and analyzed the effect which the actual operation variables cause in efficiency of process and optimized process, finally we got the DME of purity $100\%$.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.11
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• pp.1634-1637
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• 2001

Allantoin and uric acid were estimated in milk to study the association between the levels of these purine derivatives and milk production per day under given feeding regimens. Keeping the stage of lactation, parity and initial milk yield in view thirty lactating buffaloes were randomly selected from early lactating group. All the animals were fed 30 kg green, 2 kg straw and 5 kg concentrate mixture on per animal/day basis at basal level up to 8 1 produce. 1 kg concentrate mixture, soaked cotton seed and boiled cotton seed was fed for every 2 I milk, respectively in Group I (control), Group II and Group III animals. Average milk Allantoin and Uric acid levels were $120{\pm}11.7g/ml$ and $4.03{\pm}0.63g/ml$, respectively in milk. Cotton seed feeding enhanced the milk production significantly (p<0.01) in comparison to concentrate mixture fed control group animals. A significant difference (p<0.01) in milk allantoin levels was found over the different feeding management at higher level of production group animals. Study also revealed a significant negative correlation between the milk allantoin and production per day r=-0.43 (p<0.05).

• Journal of Life Science
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• v.25 no.11
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• pp.1304-1310
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• 2015

The period circadian clock gene 3 (PER3) plays a role in the mammalian circadian clocksystem. A regular exercise regime may affect the PER3 transcription in skeletal muscle. Although the effects of day length on circadian and circannual processes are well established in humans and mice, the influence of exercise on these processes in the horse has not been investigated. The present study investigated the expression of the PER3 gene following exercise in a thoroughbred breed of Korean horse. In addition, a comprehensive in silico nonsynonymous single nucleotide polymorphism (nsSNP) analysis of the horse PER3 gene and predicted effects of nsSNPs on proteins were examined. The expression of PER3 in skeletal muscle was significantly upregulated after exercise. Four nsSNPs were functionally annotated and analyzed by computational prediction. The total free energy and RMSD values of PER3 gene showed causative mutations. The results showed that nsSNP s395916798 (G72R) was associated with residues that have stabilizing effects on structure and function of PER3 gene. This study documented role of PER3 gene in phenotypic adaptation related to exercise in skeletal muscle. Further, the SNPs in PER3 could serve as useful biomarkers of early recovery after exercise in racehorses.

• Journal of Technology Innovation
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• v.17 no.1
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• pp.179-204
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• 2009

This paper aims to analyze the relationship between R&D outsourcing ratio (external R&D expenditures out of total R&D expenditures) and firm characteristics. Four hypotheses are tested using firm-level data with Tobit regression method. The major findings of this paper are as follows. First, firms with high technological capabilities, which are measured by R&D expenditures per sale and the number of patents per employee, rely more on R&D outsourcing. Nonetheless, firms that own R&D centers tend to rely less on external outsourcing of R&D. Second, firms with high managerial innovation capabilities, which is measured by the introduction of IT solutions, show higher R&D outsourcing ratio. Third, bigger firms are likely to spend more in external R&D than in internal R&D. This paper also discusses implications on R&D policy of Korea.

• Journal of Korean Library and Information Science Society
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• v.2
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• pp.1-21
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• 1975

Thc purposc of this .study is for ri~aking a model of building a library collcction dcnizn~cd on th.3 curriculum through thc inter-comparison of 15 standards for collcgc ant1 anivcrsily libraries in 5 countries (U. S. A.. Great Ikitain, Canada, Japan, and Icorca) Thc rcsult of thcscl colnpsrisons is as follow.. (1) Qu;~nlitalivc scope: of thc cull(:ction: (r/ Standards for a minimal collcction arc presented to about 100,000 vols (20-30 vols per student) for a ncw university and about 40,000 vols (40 vols pcr studcnt) for a nrnr collcge. $)$ Standards for a working collcction arc prescnted to 70-80 vols per student for a univer:aity and 50-60 vols per student for a college. $$ Standard for an intcnsive rescc~rch collcction is presented to morc than 100 vols p::r studcnt. (2) Stop: of the annual incrc;lsc cf thc collection: (i: Standard for a minimal collcction is ~~rescntcdto 1.4-2 vols per studcnt. $$ Standard for an intensive rrscarch or working collection is pcrsented to 4-5 vols pcr studcnt. (3) Scope of thc anni.1a1 budgetary standards: In Grcat I3ritain and U.S. A.. th-re aro assigned to 1 . 5 ~ 2 % of the collcge and university cxpcnditurr for books. $,$ In our country, it is prcscntcd to 3.5% of thc collcge and university expenditure for books, whcn my calculating by thc various factor analysis.

• Journal of the Korean Home Economics Association
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• v.17 no.3
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• pp.35-43
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• 1979

The survey on food intake in Masan and Ulsan city in Gyeong -Sang nam -Do was done by college students of nutrition major under the direction of nutrition professors on August 2nd, 3rd and 4th, 1976. The number of subjects were 260 family members from 54 households. The results are as follow : 1. Daily caloric intake for adults was 1670 kal which was 61.8% of R.D.A.2. The intake of protein appears to be adequate (80g per adult per day ) but fat intake was about 23g which was 5.8% of total calory intake. 3. The level of calcium and iron intake was close to that of R.D.A. However the level of vitamin A & vitamin $B_{2}$ was below R.D.A.