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Induction of Sesquiterpene Cyclase During Integrated Extraction of Sesquiterpenes from Hairy Root Cultures of Hyoscyamus muticus (Hyoscyamus muticus의 모상근배양으로부터 Sesquiterpene 화합물의 Intergration 추출시 Sesquiterpene Cyclase의 유도)

  • BACK, Kyoungwhan;SHIN, Dong Hyun;KIM, Kil Ung;De HAAS, Cynthia R.;CHAPPELL, Joseph;CURTIS Wayne R.
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.5
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    • pp.273-277
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    • 1997
  • The sesquiterpene cyclase (SC) was induced and its products were accumulated in the culture media of Hyoscyamus muticus hairy roots by addition of Rhizoctonia solani extracts. The cumulative production of solavetivone was nearly doubled by integrated extraction of the products from the media during the 24 h accumulation period. Western blots with monoclonal antibodies against SC show that the enzyme levels are the same for both extracted and non-extracted cultures. SC activities measured in vitro with radioactive substrate are not significantly different. These results suggest that productivity is controlled by substrate availability within the terpenoid pathway, and feedback regulation precedes the branch-point enzyme sesquiterpene cyclase.

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Biosynthesis of Sesquiterpene in Hairy Root and Cell Suspension Cultures of Hyoscyamus muticus by Elicitation Using Rhizoctonia solani Extracts (Rhizoctonia solani 추출액 첨가에 의한 Hyoscyamus muticus의 현탁세포배양 및 모상근배양에서 Sesquiterpene 생합성)

  • BACK, Kyoungwhan;SHIN, Dong Hyun;KIM, Kil Ung;De HAAS Cynthia R.;CHAPPELL Joseph;CURTIS Wayne R.
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.5
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    • pp.279-284
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    • 1997
  • The extracellular sesquiterpenoids were accumulated in cell and hairy root cultures of Hyoscyamus muticus by elicitation using extracts of Rhizoctonia solani. The vetispiradiene synthase (VS) which is the first committed step in biosynthetic pathway leading to formation of solavetivone, lubimin, and rishitin from isoprenoid intermediate farnesyl pyrophosphate was induced upon elicitation, whereas no sesquiterpenoids and VS activity were detected in both control cell and hairy root cultures. VS activity increased rapidly and reached its maximum 12 h in both cell and hairy root cultures upon elicitor treatment. VS activities were paralleled with the absolute levels of VS polypeptide(s). Interestingly, the profiles of sesquiterpenoid accumulation in hairy root cultures were different from those in cell cultures. The hairy root culture seemed to fail to metabolize solavetivone further to lubimin.

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Recent Development in Ultrasonic Guided Waves for Aircraft and Composite Materials

  • Rose, Joseph L.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.29 no.6
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    • pp.525-533
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    • 2009
  • Emphasis in the paper is placed on describing guided wave successes and challenges for applications in aircraft and composite materials inspection. Guided wave imaging methods discussed includes line of sight, tomography, guided wave C-scan, phased array, and ultrasonic vibration methods. Applications outlined encircles lap splice, bonded repair patch, fuselage corrosion, water loaded structures, delamination, and ice detection and de-icing of various structures.

Ignition Transient Mechanism in an Entire Integrated Rocket Ramjet Engine (램제트 엔진의 점화 천이에 관한 연구)

  • ;Vigor Yang
    • Journal of the Korean Society of Propulsion Engineers
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    • v.4 no.2
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    • pp.12-20
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    • 2000
  • The numerical analysis, including chemical reaction of an entire ramjet engine is studied to understand the ignition transient mechanism and the dynamic characteristics of the Integrated Rocket Ramjet System comprehensively. Details of how a subsonic combustion environment is established from the supersonic ram air after removal of the inlet port cover, are examined during the ignition transient. Various physical processes are investigated systemically, including ignition, flame propagation, flame dynamics, and vorticity evolution.

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Behavior of Weld Pool Shape and Weld Surface Deformation as a Function of Spot-GTA Welding Position for 304 Stainless Steel (Spot-GTA 용접자세에 따른 304 스테인리스강 용융지 표면 및 용접부 형상 거동)

  • Kang, Nam-Hyun;Park, Yeong-Do;Cho, Kyung-Mox;Singh, Jogender;Kulkarni, Anil
    • Journal of Welding and Joining
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    • v.26 no.2
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    • pp.62-68
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    • 2008
  • Effects of gravitational orientation on gas tungsten arc welding (GTAW) for 304 stainless steel were studied to determine the critical factors for weld pool formation, such as weld surface deformation and weld pool shape. This study was accomplished through an analytical study of weld pool stability as a function of primary welding parameters (arc current and arc holding time), material properties (surface tension and density), and melting efficiency (cross-sectional area). The stability of weld pool shape and weld surface deformation was confirmed experimentally by changing the welding position. The arc current and translational velocity were the major factors in determining the weld pool stability as a function of the gravitational orientation. A 200A spot GTAW showed a significant variation of the weld pool formation as the arc held longer than 3 seconds, however the weld pool shape and surface morphology for a 165A spot GTAW were 'stable', i.e., constant regardless of the gravitational orientation. The cross-sectional area of the weld (CSA) was one of the critical factors in determining the weld pool stability. The measured CSA ($13.5mm^2$) for the 200A spot GTAW showed a good agreement with the calculated CSA ($14.9mm^2$).

An instrumented glove for grasp specification in virtual reality based point-and-direct telerobotics

  • Yun, Myung Hwan;Cannon, David;Freivalds, Andris
    • Journal of the Ergonomics Society of Korea
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    • v.15 no.2
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    • pp.165-176
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    • 1996
  • Hand posture and force, which define aspects of the way an object is grasped, are features of robotic manipulation. A means for specifying these grasping "flavors" has been developed that uses an instrumented glove equipped with joint and force sensors. The new grasp specification system is being used at the Pennsylvania State University (Penn State) in a Virtual Reality based Point-and-Direct (VR-PAD) robotics implementation. In the Computer Integrated Manufacturing (CIM) Laboratory at Penn State, hand posture and force data were collected for manipulating bricks and other items that require varying amounts of force at multiple pressure points. The feasibility of measuring desired grasp characteristics was demonstrated for a modified Cyberglove impregnated with FSR (Force Sensitive Resistor) pressure sensors in the fingertips. A joint/force model relating the parameters of finger articulation and pressure to various lifting tasks was validated for the instrumented "wired" glove. Operators using such a modified glove may ultimately be able to configure robot grasping tasks in environments involving hazardous waste remediation, flexible manufactruing, space operations and other flexible robotics applications. In each case, the VR-PAD approach improved the computational and delay problems of real-time multiple- degree-of-freedom force feedback telemanipulation.

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Water Quality Modeling in the Delaware River Basin by SWAT(Soil and Water Assessment Tools) (SWAT를 이용한 델라웨어강 유역의 수질모델링)

  • Cho, Sung-Min;Lee, Myung-Woo
    • Journal of Environmental Impact Assessment
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    • v.4 no.2
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    • pp.39-57
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    • 1995
  • The water quality model SWAT (Soil and Water Assessment Tool) was used in combination with GIS, Arc/Info and GRASS, to evaluate land use impacts in the Delaware River Basin in Pennsylvania. This paper describes application of GIS with the water quality model in the 250 square kilometer Brodhead Creek Watershed. Date used in water quality modeling include 1:250,000 digital elevation models (DEM), soil data, and monitored streamflow and curve numbers, and other input variables.

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Improvements to the stability of electric field sensors

  • Lee, Dong-Oh;Robert Boston;Dietrich W. Langer;Joel Falk
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.06a
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    • pp.495-496
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    • 1998
  • The measurement of the amplitude and phase of electric fields on high voltage transmission lines is important for several reasons including a) Metering and determination of power flow, b) protective relaying. and c) fault sensing. The work reported here is directed toward a major improvement to optically based, electric-field sensors. This is a signal processing based technique for overcoming the instabilities of conventional, optically-based, electric-field sensors to changes in optical power or temperature.

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Anaerobic Metabolism of the Herbicide, Butachlor in soil (토양중에 있어서 제초제 Butachlor의 혐기적 대사)

  • Lee, Jae-Koo;Minard, Robert D.;Bollag, Jean-Marc
    • Applied Biological Chemistry
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    • v.25 no.2
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    • pp.83-92
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    • 1982
  • In an anaerobic incubation of uniformly ring-labeled $^{14}C-butachlor$ in two Korean soils for 3 months, very little $^{14}CO_2$ and volatile products were measured. In soil A, 77.52 and 45.36% of the sterile and viable soil radioactivity, respectively, were methanol-extractable and the rest were adsorbed in soil; whereas in soil B, 58.85 and 37.23%, respectively, were methanol-extractable and the rest remained in soil. The adsorption of $^{14}C-butachlor$ depends on the characteristics of the soils. The major metabolite was 2,6-diethyl-N-(butoxymethyl) acetanilide. 2,6-Diethylaniline and 2,6-diethylacetanilide turned out to be the minor metabolites on GLC-MS.

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Mineral-Based Slow Release Fertilizers: A Review

  • Noh, Young Dong;Komarneni, Sridhar;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.1
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    • pp.1-7
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    • 2015
  • Global population is expected to reach nine billion in 2050 and the total demand for food is expected to increase approximately by 60 percent by 2050 as compared to 2005. Therefore, it is important to increase crop production in order to meet the global demand for food. Slow release fertilizers have been developed and designed in order to improve the efficiency of fertilizers. Mineral-based slow release fertilizers are useful because the minerals have a crystalline structure and are environmentally friendly in a soil. This review focuses on slow release fertilizers based on montmorillonite, zeolite, and layered double hydroxide phases as a host for nutrients, especially N. Urea was successfully stabilized in the interlayer space of montmorillonite by the formation of urea-Mg or Ca complex, $[(Urea)_6Mg\;or\;Ca]^{2+}$ protecting its rapid degradation in soils. Naturally occurring zeolites occluded with ammonium nitrate and potassium nitrate by molten salt treatment could be used as slow release fertilizer because the occlusion process increased the capacity of zeolites to store nutrients in addition to exchangeable cations. Additionally, surface-modified zeolites could also be used as slow release fertilizer because the modified surface showed high affinity for anionic nutrients such as nitrate and phosphate. Moreover, there were attempts to develop and use synthetic layered double hydroxide as a carrier of nitrate because it has positively charged layers which electrostatically bond nitrate anions. Kaolin was also tested by combining with a polymer or through the mechanical-chemical process for slow release of nutrients.