• 월간양계
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• 통권122호
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• pp.111-122
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• 1979

• Bulletin of the Korean Chemical Society
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• 제21권12호
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• pp.1199-1201
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• 2000

31-Hydroxycarbowyacetylquercinic acid, a lanostenoid hydroxyl acid has been isolated from D. dickinsii by solbent extraction, silica gel column chromatography and recrystallization. The structure of this compound has been determined to be 31-hydroxycarboxyacetylquercinic acid by a combinationof spectral data and by HM-BC. This compound showed antimicrobial activities against human pathogenic fungi and bacteria.

• 생명과학회지
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• 제32권12호
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• pp.1013-1013
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• 2022

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2002년도 총회 및 추계학술발표회
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• pp.56.2-57
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• 2002

• 한국식품위생안전성학회지
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• 제18권4호
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• pp.229-236
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• 2003

본 연구는 식품을 오염시켜 식중독을 유발하는 주요 식중독 세균인 Escherichi, Salmonella, Shigella, Vibrio, Listeria등 5속의 병원성 세균들을 반복성 염기서열을 이용한 ERIC-PCR을 이용하여 동시에 동정할 때 이용되는 주요 PCR 반응성분인 $MgCl_2$, dNTPs, primers, template DNA의 최적 농도를 결정하였다. ,$MgCl_2$ 반응성분은 2 mM을 적용하였을 때부터 일정한 fingerprinting pattern을 얻을 수 있었으므로 2 mM을 $MgCl_2$의 최적농도로 결정하였다. dNTPs의 농도는 250 ${\mu}M$까지 증가함에 따라 6균주 모두 200 ${\mu}M$까지는 농도 증가와 비례하여 단편의 수와 강도가 점증하였으나 그 이상의 농도에서는 단편의 수와 강도가 일정하였다. 그러므로 일정한 ,fingerprinting pattern 을 얻기 위하여 200 ${\mu}M$의 dNTPs만으로도 충분하였다. ERIC primer들은 2 ${\mu}M$의 농도를 적용했을 때부터 일정한 fingerprinting pattern을 나타낼 수 있었으며, 그 이상의 농도를 적용했을 때 단지 단편들의 강도만이 증가하였다. Template DNA도 다른 PCR 반응성분에 대한 실험과 마찬가지로 적용한 DNA 양의 증가에 따라 단편의 간도가 증가하였다. 그러나 대부분의 적용 균주들에 대하여 DNA의 양이 최고일 때와 최대일 때 각각의 얻어지 fingerprinting pattern들을 비교할 때 단편의 수는 별 차이가 없었다.

• Journal of Animal Science and Technology
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• 제53권1호
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• pp.67-74
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• 2011

본 연구는 한우, 젖소, 돼지, 산란계 HACCP 적용 농장의 분변, 원유 및 사육환경에서 병원성세균 등 분석을 통하여 HACCP 적용 농장의 생물학적 위해 관리 실태를 파악하고자 하고 이를 농장 HACCP 시스템 적용에 관한 기초 연구 자료로 사용하는 것을 목적으로 하였다. 미생물 시료는 HACCP을 적용 중인 한우, 젖소, 돼지, 산란계농장의 축사바닥분뇨, 퇴비사, 원유탱크, 집란기, 분변 벨트 등에서 채취 하였으며 축산식품의 생물학적 위해인 Pathogenic E. coli, Salmonella spp. S. aureus 등을 분석하였다. 각 축종별 대장균은 분변에서 100% 검출되었으나 Salmonella spp.는 검출되지 않았다. 또한 젖소농장의 원유 냉각기에서도 S. aurues가 검출되지 않았다. 그러나 돼지농장의 돈사바닥 및 퇴비장에서는 검사건수 30건당 각각 2건, 1건의 Salmonella spp.가 검출되었다. 마찬가지로 산란계농장의 계사바닥 및 분변벨트에서도 전체분석 건수 대비 10~20%의 Salmonella spp.가 검출되었다. 이러한 결과는 문헌상 보고되고 있는 일반축산농가의 병원성세균 관리실태와 간접 비교하였을 경우 HACCP 시스템을 적용중인 축산농가에서의 일반축산농가보다 병원성 세균관리가 더욱 철저히 이루어지고 있는 것으로 판단된다.

• Toxicological Research
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• 제29권2호
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• pp.129-135
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• 2013

Salmonella is one of the major pathogenic bacteria that cause food poisoning. This study investigated whether heat-killed as well as live Lactobacillus protects host animal against Salmonella infection. Live and heat-killed Lactobacillusacidophilus was administered orally to Sprague-Dawley rats for 2 weeks before the rats were inoculated with Salmonella. Rise in body temperature was moderate in the group that was treated with heat-killed bacteria as compared to the Salmonella control group. The mean amount of feed intake and water consumption of each rat in the heat-killed bacteria group were nearly normal. The number of fecal Salmonellae was comparable between the live and the heat-killed L. acidophilus groups. This finding shows that L. acidophilus facilitates the excretion of Salmonella. Moreover, the levels of pro inflammatory cytokines, including tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta, in the heat-killed L. acidophilus group were significantly lower when compared to the levels in the Salmonella control group. These results indicate that nonviable lactic acid bacteria also could play an important role in preventing infections by enteric pathogens such as Salmonella.

• 한국축산식품학회지
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• 제35권2호
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• pp.272-276
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• 2015

The microbial distribution of raw materials and beef jerky, and the effect of nisin on the growth of Bacillus cereus inoculated in beef jerky during storage, were studied. Five strains of pathogenic B. cereus were detected in beef jerky, and identified with 99.8% agreement using API CHB 50 kit. To evaluate the effect of nisin, beef jerky was inoculated with approximately 3 Log CFU/g of B. cereus mixed culture and nisin (100 IU/g and 500 IU/g). During the storage of beef jerky without nisin, the number of mesophilic bacteria and B. cereus increased unlikely for beef jerky with nisin. B. cereus started to grow after 3 d in 100 IU nisin/g treatment, and after 21 d in 500 IU nisin/g treatment. The results suggest that nisin could be an effective approach to extend the shelf-life, and improve the microbial safety of beef jerky, during storage.

• Journal of Plant Biotechnology
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• 제37권3호
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• pp.327-336
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• 2010

Cysteine proteases have been known as a critical factor in plant defense mechanisms in pineapple, papaya, or wild fig. Papain or ficin is one kind of cysteine proteases that shows toxic effects to herbivorous insects and pathogenic bacteria. However, resistance to bacterial soft rot of plants genetically engineered with cysteine protease has been little examined thus far. We cloned a cysteine protease cDNA from Ananas comosus and introduced the gene into Chinese cabbage (Brassica rapa) under the control of the cauliflower mosaic virus 35S promoter. The transgene was stably integrated and actively transcribed in transgenic plants. In comparisons with wild-type plants, the $T_2$ and $T_3$ transgenic plants exhibited a significant increase in endo-protease activity in leaves and enhanced resistance to bacterial soft rot. A cDNA microarray analysis revealed that several genes were more abundantly transcribed in the transgenic than in the wild type. These genes encode a glyoxal oxidase, PR-1 protein, PDF1, protein kinase, LTP protein, UBA protein and protease inhibitor. These results suggest an important role for cysteine protease as a signaling regulator in biotic stress signaling pathways, leading to the build-up of defense mechanism to pathogenic bacteria in plants.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.968-978
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• 2007

A pathogenic nematode, Butlerius sp., was isolated from Oriental beetle, Blitopertha orientalis. The infective juveniles exhibited dose-as well as time-dependent entomopathogenicity on the larvae of B. orientalis. Two bacterial species, Providencia vermicola (KACC 91278) and Flavobacterium sp. (KACC 91279), were isolated from the infective juveniles and identified. P. vermicola outnumbered Flavobacterium sp. in the nematode host, in which the colony density of P. vermicola was found to be 21 times higher than that of Flavobacterium sp. However, when the two bacterial species were cocultured in culture media without the nematode host, they showed similar growth rates. Both bacteria induced significant entomopathogenicity against Spodoptera exigua larvae infesting economically important vegetable crops, where P. vermicola was more potent than Flavobacterium sp.