Browse > Article
http://dx.doi.org/10.5487/TR.2013.29.2.129

A Study on the Prevention of Salmonella Infection by Using the Aggregation Characteristics of Lactic Acid Bacteria  

Kim, Min-Soo (R&D Center, Cellbiotech, Co. Ltd.)
Yoon, Yeo-Sang (R&D Center, Cellbiotech, Co. Ltd.)
Seo, Jae-Gu (R&D Center, Cellbiotech, Co. Ltd.)
Lee, Hyun-Gi (R&D Center, Cellbiotech, Co. Ltd.)
Chung, Myung-Jun (R&D Center, Cellbiotech, Co. Ltd.)
Yum, Do-Young (R&D Center, Cellbiotech, Co. Ltd.)
Publication Information
Toxicological Research / v.29, no.2, 2013 , pp. 129-135 More about this Journal
Abstract
Salmonella is one of the major pathogenic bacteria that cause food poisoning. This study investigated whether heat-killed as well as live Lactobacillus protects host animal against Salmonella infection. Live and heat-killed Lactobacillusacidophilus was administered orally to Sprague-Dawley rats for 2 weeks before the rats were inoculated with Salmonella. Rise in body temperature was moderate in the group that was treated with heat-killed bacteria as compared to the Salmonella control group. The mean amount of feed intake and water consumption of each rat in the heat-killed bacteria group were nearly normal. The number of fecal Salmonellae was comparable between the live and the heat-killed L. acidophilus groups. This finding shows that L. acidophilus facilitates the excretion of Salmonella. Moreover, the levels of pro inflammatory cytokines, including tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta, in the heat-killed L. acidophilus group were significantly lower when compared to the levels in the Salmonella control group. These results indicate that nonviable lactic acid bacteria also could play an important role in preventing infections by enteric pathogens such as Salmonella.
Keywords
Salmonella; Lactobacillus acidophilus; Food poisoning; Heat-killed bacteria; Probiotics;
Citations & Related Records
연도 인용수 순위
  • Reference
1 McClelland, M., Sanderson, K.E., Spieth, J., Clifton, S.W., Latreille, P., Courtney, L., Porwollik, S., Ali, J., Dante, M., Du, F., Hou, S., Layman, D., Leonard, S., Nguyen, C., Scott, K., Holmes, A., Grewal, N., Mulvaney, E., Ryan, E., Sun, H., Florea, L., Miller, W., Stoneking, T., Nhan, M., Waterston, R. and Wilson, R.K. (2001) Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature, 413, 852-856.   DOI   ScienceOn
2 Mennechet, F.J., Kasper, L.H., Rachinel, N., Li, W., Vandewalle, A. and Buzoni-Gatel, D. (2002) Lamina propria CD4+ T lymphocytes synergize with murine intestinal epithelial cells to enhance proinflammatory response against an intracellular pathogen. J. Immunol., 168, 2988-2996.   DOI
3 Hack, C.E., Aarden, L.A. and Thijs, L.G. (1997) Role of cytokines in sepsis. Adv. Immunol., 66, 101-195.   DOI
4 Bhutta, Z.A., Mansoorali, N. and Hussain, R. (1997) Plasma cytokines in paediatric typhoidal salmonellosis: correlation with clinical course and outcome. J. Infect., 35, 253-256.   DOI   ScienceOn
5 Kim, T.S., Hur, J.W., Yu, M.A., Cheigh, C.I., Kim, K.N., Hwang, J.K. and Pyun, Y.R. (2003) Antagonism of Helicobacter pylori by bacteriocins of lactic acid bacteria. J. Food Prot., 66, 3-12.   DOI
6 Park, J.H., Lee, Y., Moon, E., Seok, S.H., Cho, S.A., Baek, M.W., Lee, H.Y., Kim, D.J. and Park, J.H. (2005) Immunoenhancing effects of a new probiotic strain, Lactobacillus fermentum PL9005. J. Food Prot., 68, 571-576.   DOI
7 Handley, P.S., Harty, D.W., Wyatt, J.E., Brown, C.R., Doran, J.P. and Gibbs, A.C. (1987) A comparison of the adhesion, coaggregation and cell-surface hydrophobicity properties of fibrillar and fimbriate strains of Streptococcus salivarius. J. Gen. Microbiol., 133, 3207-3217.
8 Kumar, A., Henderson, A., Forster, G.M., Goodyear, A.W., Weir, T.L., Leach, J.E., Dow, S.W. and Ryan, E.P. (2012) Dietary rice bran promotes resistance to Salmonella enteric serovar Typhimurium colonization in mice. BMC Microbiol., 12, 71.   DOI
9 Collado, M.C., Surono, I., Meriluoto, J. and Salminen, S. (2007) Indigenous dadih lactic acid bacteria: cell-surface properties and interactions with pathogens. J. Food Sci., 72, M89-M93.   DOI   ScienceOn
10 Jacobsen, C.N., Rosenfeldt Nielsen, V., Hayford, A.E., Møller, P.L., Michaelsen, K.F., Paerregaard, A., Sandstrom, B., Tvede, M. and Jakobsen, M. (1999) Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl. Environ. Microbiol., 65, 4949-4956.
11 Lievin-Le Moal, V., Amsellem, R., Servin, A.L. and Coconnier, M.H. (2002) Lactobacillus acidophilus (strain LB) from the resident adult human gastrointestinal microflora exerts activity against brush border damage promoted by a diarrhoeagenic Escherichia coli in human enterocyte-like cells. Gut, 50, 803-811.   DOI   ScienceOn
12 Ishikawa, H., Kutsukake, E., Fukui, T., Sato, I., Shirai, T., Kurihara, T., Okada, N., Danbara, H., Toba, M., Kohda, N., Maeda, Y. and Matsumoto, T. (2010) Oral administration of heat-killed Lactobacillus plantarum strain b240 protected mice against Salmonella enterica Serovar Typhimurium. Biosci. Biotechnol. Biochem., 74, 1338-1342.   DOI   ScienceOn
13 Rodriguez, I., Rodicio, M.R., Guerra, B. and Hopkins, K.L. (2012) Potential international spread of multidrug-resistant invasive Salmonella enterica serovar enteritidis. Emerg Infect. Dis., 18, 1173-1176.   DOI
14 Maisnier-Patin, S., Berg, O., Liljas, L. and Andersson, D.I. (2002) Compensatory adaptation to the deleterious effect of antibiotic resistance in Salmonella typhimurium. Mol. Microbiol., 46, 355-366.   DOI   ScienceOn
15 Pelto, L., Isolauri, E., Lilius, E.M., Nuutila, J. and Salminen, S. (1998) Probiotic bacteria down-regulate the milk-induced inflammatory response in milk-hypersensitive subjects but have an immunostimulatory effect in healthy subjects. Clin. Exp. Allergy, 28, 1474-1479.   DOI   ScienceOn
16 van Eerden, E., van den Brand, H., De Vries Reilingh, G., Parmentier, H.K., de Jong, M.C. and Kemp, B. (2004) Residual feed intake and its effect on Salmonella enteritidis infection in growing layer hens. Poult. Sci., 83, 1904-1910.   DOI
17 Wang, K., Waselenchuk, L. and Evered, M.D. (1993) Stimulation of drinking by bacterial endotoxins in the rat. Physiol. Behav., 54, 1005-1009.   DOI   ScienceOn
18 Arvizu-Medrano, S.M. and Escartín, E.F. (2005) Effect of acid shock with hydrochloric, citric, and lactic acids on the survival and growth of Salmonella typhi and Salmonella typhimurium in acidified media. J. Food Prot., 68, 2047-2053.   DOI
19 Santos, R.L., Zhang, S., Tsolis, R.M., Kingsley, R.A., Adams, L.G. and Baumler, A.J. (2001) Animal models of Salmonella infections: enteritis versus typhoid fever. Microbes Infect., 3, 1335-1344.   DOI   ScienceOn
20 Tsolis, R.M., Young, G.M., Solnick, J.V. and Baumler, A.J. (2008) From bench to bedside: stealth of enteroinvasive pathogens. Nat. Rev. Microbiol., 6, 883-892.   DOI   ScienceOn
21 Banerjee, S., Ooi, M.C., Shariff, M. and Khatoon, H. (2012) Antibiotic resistant Salmonella and Vibrio associated with farmed Litopenaeusvannamei. Sci. World J., 2012, 130-136.
22 Van Meervenne, E., Van Coillie, E., Kerckhof, F.M., Devlieghere, F., Herman, L., De Gelder, L.S., Top, E.M. and Boon, N. (2012) Strain-specific transfer of antibiotic resistance from an environmental plasmid to food borne pathogens. J. Biomed. Biotechnol., 2012, 834598.