• Ocean and Polar Research
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• 제33권2호
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• pp.159-169
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• 2011

Antifreeze proteins (AFPs) have ice binding affinity, depress freezing temperature and inhibit ice recystallization which protect cellular membranes in polar organisms. Recent structural studies of antifreeze proteins have significantly expanded our understanding of the structure-function relationship and ice crystal growth inhibition. Although AFPs (Type I-IV AFP from fish, insect AFP and Plant AFP) have completely different fold and no sequence homology, they share a common feature of their surface area for ice binding property. The conserved ice-binding sites are relatively flat and hydrophobic. For example, Type I AFP has an amphipathic, single ${\alpha}$-helix and has regularly spaced Thr-Ala residues which make direct interaction with oxygen atoms of ice crystals. Unlike Type I AFP, Type II and III AFP are compact globular proteins that contain a flat ice-binding patch on the surface. Type II and Type III AFP show a remarkable structural similarity with the sugar binding lectin protein and C-terminal domain of sialic acid synthase, respectively. Type IV is assumed to form a four-helix bundle which has sequence similarity with apolipoprotein. The results of our modeling suggest an ice-binding induced structural change of Type IV AFP. Insect AFP has ${\beta}$-helical structure with a regular array of Thr-X-Thr motif. Threonine residues of each Thr-X-Thr motif fit well into the ice crystal lattice and provide a good surface-surface complementarity. This review focuses on the structural characteristics and details of the ice-binding mechanism of antifreeze proteins.

• Journal of Pharmaceutical Investigation
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• 제41권1호
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• pp.9-17
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• 2011

The objective of this work is to study transdermal delivery of calcitonin using iontophoresis and to evaluate various factors which affect the transdermal transport. We have studied the effect of polarity, current density, drug concentration, penetration enhancers (isopropyl myristate [IPM] and ethanol) and laser treatment on transdermal flux and the results were compared. We also investigated the iontophoretic flux from microemulsions containing calcitonin together with oleic acid (OA) or IPM. In vitro flux study was performed at $33^{\circ}C$, using side-by-side diffusion cell and full thickness hairless mouse skin. Anodal delivery at pH 3.0 was much larger than cathodal and passive delivery, due to the positive charge of calcitonin. Cumulative amount delivered (CUM) by cathodal or passive delivery was close to zero for 10 hours. The pretreatment of skin by neat IPM markedly increased the CUM anodically. CUM increased as the current density, drug concentration or the duration of IPM treatment increased. Microemulsion containing IPM or oleic acid was prepared and the phase diagram was constructed. CUM also increased when IPM was incorporated into a microemulsion. OA microemulsion showed similar enhancing effect to IPM microemulsion. The delivery of calcitonin from 70% (v/v) ethanol aqueous solution showed a large increase in flux. Laser treatment of skin before flux experiment exhibited about 2 fold increase in total calcitonin amount transported for 12 hours, when compared to that delivered by IPM microemulsion. Based on these results, we have evaluated the possibility of delivering enough amount of calcitonin to reach the therapeutic level. The data suggest that it is highly possible to deliver clinically effective amount of calcitonin using iontophoresis patch with small area (<10 $cm^2$).

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.175-175
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• 1996

The spinal dorsal horn is the area where primary afferent fibers terminate and cutaneous sensory information is Processed. A number of putative neurotransmitter substances, including excitatory and inhibitory amino acids and peptides, are present in this region and sites and cellular mechanisms of their actions have been a target of numerous studies. In this study, single neurons were acutely isolated and the properties of whole cell current and responses to excitatory and inhibitory neurotransmitters were studied by the patch clamp method. Young rats (7-14 days) were anesthetized with diethyl-ether, and the lumbar spinal cord was excised and cut transversely at a thickness of 30$\mu\textrm{m}$ by Vibroslicer. The treatment of spinal slices with low concentration of proteases (pronase and thermolysin 0.75 mg/$m\ell$) and mechanical dissociation yielded isolated neurons with near intact morphology. Multipolar, ellipsoidal and bipolar, and pyramidal cells were shown. By applying step voltage pulses to neurons held at -70 mV, two types of inward currents and one outward currents observed. The fast activating and inactivating inward current was the Na$\^$+/ current because of its fast kinetics and blocking by 0.5${\mu}$M TTX, a specific blocker of Na$\^$+/ channel. The second type of inward currents were sustained. Based on their kinetics and current-voltage relations, it was likely that the second type of inward current was the voltage-dependent Ca$\^$2+/ current. In the presence of TTX, the steady-state currents mainly represented outward K$\^$+/ current which looked like the delayed rectifier K$\^$+/ current. In addition, the membrane currents produced by agonist of excitatory amino acid (EAA) receptor and the endogenous transmitter candidate L-glutamate were recorded in isolated whole-cell voltage clamped neurons as well as responses to inhibitory amino acids (${\gamma}$-amino butyric acid, glycine). Drugs were applied by a method that allows complete exchange of the solution within 1 sec; an infinite number of solutions can be applied to a single cell.

• 한국전자파학회논문지
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• 제20권12호
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• pp.1316-1324
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• 2009

본 논문은 충분한 대역폭과 이득을 갖는 5.8 GHz 대역의 무선 LAN용 십자형 개구 결합 마이크로스트립 원형 편파 $2\times2$배열 안테나를 설계 제작하였다. 설계한 안테나는 정사각형 패치를 사용하고 직렬 형태의 급전 선로위에 $\lambda_g$/4 위상차가 나는 곳에 슬롯이 위치하게 하여 전류 방향이 최대 최소가 반복되게 하여 원형 편파를 발생시키게 된다. 기존의 십자형 슬롯 안테나 구조를 수정하여 두 개의 슬롯이 직각으로 교차하는 부분을 없애고 분리된 4개의 슬롯으로 안테나를 설계함으로써 후방 방사를 줄이고 배열을 통해 안테나의 지향성도 개선할 수 있다. 측정된 $2\times2$ 배열 안테나의 대역폭은 5.67~5.95 GHz로 나타났으며, 최대 방사 이득은 10.59 dBi를 얻었다.

• 한국조경학회지
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• 제35권1호
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• pp.79-87
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• 2007

The objective of this study was to delineate forest patches in the cities around the Geum-buk mountains at the north of the Geum River using multiplesatellite remote sensing data. Landsat visible and near-infrared satellite images obtained at multiple dates in the growing season were used to create a forest distribution map. Fragstats 3.3 was used to get the landscape indices delineating the distribution of forest patches. Additional ground truth data was used to assess the accuracy of the classification. Factor analysis was used to get the 26 landscape indices clustered into 4 factors. Factor I was labeled as' size of forest patches', factor II as 'fragmentation of forest patches', factor III as 'shape of forest patches', and factor IV as 'complexity of forest patches'. Factor I described large patches and their core area, while others did small patches and their shape and complexity. Cities including Cheonan, Gongju, Cheongyang, and Boryeong near the main ridge of the Geumbuk Mtns. had a small number of large-sized forest patches. However, cities including Taean, Seosan, Dangjin, Hongseong near the ridge of the western Geumbuk Mtns. had a large number of small-sized forest patches. Finally, this study showed that the region near the coast line in Chung-nam province has various types of forest patches having an irregular forest edge due to the elevation and slope lower than the one of the region far from the coast line which is near the ridge of the Geum-buk Mountains. Remote sensing data were useful to understand the distribution of forest patches, and landscape indices could be keys to delineate the relationship between forest patches. And the factor analysis, which simplified 26 landscape indices into 4 landscape patterns allowed us to understand the distribution and relationship of forest patches in an easy way.

• 한국항공우주학회지
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• 제42권7호
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• pp.567-575
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• 2014

본 논문에서는 스마트 스킨 시제품을 항공기에 장착하고 비행데모시험을 수행하는 절차와 시험결과를 제시하였다. 통신항법용 4개의 안테나를 한 개의 안테나 내장 스킨구조(CLAS)에 삽입하였다. 대수주기 패치형 안테나를 4개의 안테나 주파수 대역을 포함하도록 다중대역 안테나로 설계하였다. 항공기에 장착하기 전에 안테나 내장 스킨구조의 요구조건은 지상시험으로 입증하였다. CLAS 스피드브레이크를 KT-1 항공기에 장착하고 2개 이상의 안테나를 작동시키는 다중 안테나 시험을 지상에서 수행하였다. 항공기에 장착된 기존 장비들과 CLAS와의 호환성을 확인하기 위해 전자파 적합성시험을 수행하였다. 비행데모시험은 4개의 안테나에 대하여 각 1회의 비행을 수행하였다. 비행 중 안테나 통신항법 신호의 작동상태와 지속성, 항공기 원형 비행 시 사각지대 유무 확인을 하였다. 안테나 내장 스킨구조의 안테나는 4회 비행데모시험 동안 기대 이상의 성능을 보여주었다.

• 한국환경복원기술학회지
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• 제4권2호
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• pp.52-61
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• 2001

At least, there are intense pressures on the natural habitats from various disturbance, including urbanization, extension of industrial area, and road construction. These human land use result in fragmentation of landscape and natural habitat. The ecological consequences of habitat fragmentation include the direct effects of habitat loss and the indirect effects of reduced inter-patch dispersal. The decline of biological diversity has been rapidly declined by the habitat loss and fragmentation. Conservation strategists should consider not only the habitat amount of that must be preserved, but also the spatial configuration of habitat across the landscape. But, the paucity of available data for most species forces landscape ecologists to develop the concept, model, and theory. The developed theories are often misused in academic papers and practical applications. The development history, presumption of concept, model, theory is ignored. This tendency have leaded to failure of landscape restoration and the use of theory in conservation practice have come under increasing attacks. This paper will highlight the ecological theory that have proven the most influential in landscape ecology, restoration and conservation : the theory of island biogeography, the theory of nested subset analysis, minimum viable population(MVP), the theory of metapopulation dynamics. And, it find the problem and usefulness of four theory in application to real world. Consequently, the understanding of theoretical implication about landscape ecological theory is required. We must carefully apply the theory after examining the problem and availability of various theory because of no existence of only one general theory.

• Journal of Chest Surgery
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• 제29권4호
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• pp.440-443
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• 1996

삼첨 판막 심내막염은 감수성이 있는 항생제에 효과적으로 치료될 수 있다. 그러나 패혈증이 지속되거 나, 중증의 심부전, 다발성 폐 색전증 그리고 실 초음파상 증식증 (vegetation)이 있는 경우는 수술이 필요 하다. . 19세횝 남자환자가 감염성심내막염으로 입원하였다. 환자는 9년전에 perimembranous type의 VSD로 수술받은 병 력 이 있다. 심초음파상 삼첨판의 전엽부에 커다란 증식증의 소견이 보였고,과거에 VSD를 봉합했던 부위에 누 출 (leakage)을 통한 좌우단락이 관찰되었다. 항생제치료와 함께 인공판막치 환술을 시행하였다. St. Jude Medical 양엽판막 (size 33 mm으로 삼첨 판막 대 치술을 시 행하였고 잔여 VSD는 단순봉합으 로 폐쇄하였다. 술후 시행한 심초음파검사에서 치환된 삼첨 판막의 기능은 좋았고, 증식증과 좌우단락의 소견은 보이지 않았다. 환자는 합병증없이 술후 25일째에 퇴원하였다. 저자들이 경험한 본 증례는 잔여 VSD로 인해 생긴 감염성심내막염을 초기에 적극적인 수술을 시행 하여 좋은결과를 얻었음을 보여준다. 이 에 문헌고찰과 더불어 보고하는 바이다.

• 대한전자공학회논문지TC
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• 제41권6호
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• pp.93-99
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• 2004

무선랜에 대한 수요의 급격한 증가로 HyperLAN(5.1-533GHz, 5.725-5.825GHz)이 전세계 적으로 무선랜을 위하여 새롭게 할당되고 있다. 또한 대부분의 이동통신기기는 크기가 작으므로 안테나의 크기는 효과적인 설계를 위해 매우 중요하다. 본 논문에서는 5GHz 무선랜을 위해 소형 광대역 안테나를 설계하였다. U슬롯 안테나는 간단한 구조이지만 $10-40\%$의 광대역을 가지고 있으며, 또한 좋은 방사특성을 가지고 있다. 그러나, 일반적으로 U슬롯 안테나의 크기는 크므로 PDA나 노트북과 같은 무선통신기기에 적용하기에 어려움이 있다. 제안된 안테나는 사각패치에 U형 슬롯을 이용하여 광대역 특성을 얻었고, 두 개의 유전체 기판과 스티로폼을 이용함으로써 소형화하였다. U슬롯 안테나의 설계를 위해 유한차분시간영역(FDTD) 법이 적용되었고 MPI 병렬화기법을 이용하여 FDTD법의 분석속도를 향상하였다.

• 대한화장품학회지
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• 제25권4호
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• pp.145-154
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• 1999

A novel retinol derivative, polyethoxylated retinamide(Medimin A) was synthesized, as an anti-aging agent. Collagen synthesis, skin permeation, stability, and toxicity of Medimin A were evaluated and compared with those of retinol and retinyl palmitate. In vitro collagen synthesis was evaluated by quantitative assay of $[^3H]-proline$ incorporation into collagenase sensitive protein in fibroblast cultures. For in vitro skin permeation experiments, Franz diffusion cells(effective diffusion area: 1,766 $cm^2$) and the excised skin of female hairless mouse aged 8 weeks were used, The stabilities of retinoids were evaluated at two different temperature($25^{\circ}C\;and\;40^{\circ}C$) and under UV in solubilized state and in O/W emulsion. To estimate the safety, acute oral toxicity, acute dermal toxicity, primary skin irritation, acute eye irritation and human patch test were performed. The effect of Medimin A on collagen synthesis was similar to that of retinol. The skin permeability of Medimin A was higher than those of retinol and retinyl palmitate. The Medimin A was more stable than retinol and retinyl palmitate. Medimin A was nontoxic in various toxicological tests. These results suggest that Medimin A would be a good anti-aging agent for enhancing bioavailability and stability.