• 제목/요약/키워드: Passive resistance

Search Result 330, Processing Time 0.027 seconds

Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa

  • Li, Guoliang;Zhang, Shifan;Li, Fei;Zhang, Hui;Zhang, Shujiang;Zhao, Jianjun;Sun, Rifei
    • The Plant Pathology Journal
    • /
    • v.37 no.1
    • /
    • pp.47-56
    • /
    • 2021
  • Plants protect against viruses through passive and active resistance mechanisms, and in most cases characterized thus far, natural recessive resistance to potyviruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimolecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and translation initiation factors to reveal the working of key amino acids.

A Study on Chloride Threshold Level of Blended Cement Mortar Using Polarization Resistance Method (분극저항 측정기법을 이용한 혼합 시멘트 모르타르의 임계 염화물 농도에 대한 연구)

  • Song, Ha-Won;Lee, Chang-Hong;Lee, Kewn-Chu;Ann, Ki-Yong
    • Journal of the Korea Concrete Institute
    • /
    • v.21 no.3
    • /
    • pp.245-253
    • /
    • 2009
  • The importance of chloride ions in the corrosion of steel in concrete has led to the concept for chloride threshold level (CTL). The CTL can be defined as the content of chlorides at the steel depth that is necessary to sustain local passive film breakdown and hence initiate the corrosion process. Despite the importance of the CTL, due to the uncertainty determining the actual limits in various environments for chloride-induced corrosion, conservative values such as 0.4% by weight of cement or 1.2 kg in 1 $m^3$ concrete have been used in predicting the corrosion-free service life of reinforced concrete structures. The paper studies the CTL for blended cement concrete by comparing the resistance of cementitious binder to the onset of chloride-induced corrosion of steel. Mortar specimens were cast with centrally located steel rebar of 10 mm in diameter using cementitious mortars with ordinary Portland cement (OPC) and mixed mortars replaced with 30% pulverized fuel ash (PFA), 60% ground granulated blast furnace slag (GGBS) and 10% silica fume (SF), respectively, at 0.4 of a free W/B ratio. Chlorides were admixed in mixing water ranging 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.5, 2.0, 2.5 and 3.0% by weight of binder(Based on $C1^-$). Specimens were curd 28 days at the room temperature, wrapped in polyethylene film to avoid leaching out of chloride and hydroxyl ions. Then the corrosion rate was measured using the polarization resistance method and the order of CTL for binder was determined. Thus, CTL of OPC, 60%GGBS, 30%PFA and 10%SF were determined by 1.6%, 0.45%, 0.8% and 2.15%, respectively.

Resistance to Naegleria fowleri infection passively acquired from immunized splenocyte, serum or milk (면역시킨 마우스의 비장세포, 혈청 또는 모유를 통해 얻을 수 있는 Naegleria fowleri 감염에 대한 방어 능력)

  • 안명희;민득영
    • Parasites, Hosts and Diseases
    • /
    • v.27 no.2
    • /
    • pp.79-86
    • /
    • 1989
  • A pathogenic free-living amoeba, Naegleria fowleri, causes primary amoebic meningoencephalitis to human and experimental animals. This infection is rare, but the mortality is very high. Nowadays, drug treatment or active immunization of human or mice are being tried with partial effectiveness. This study shows passive immunization effect by transfer of immunized spleen cells, serum, or milk from immunized mother in mouse experimental model. Young BALB/c mice were immunized intraperitoneally with $2~3{\times}10^{6}$ trophozoites of N. fowleri, and spleen cells and sera were collected for injection to recipient mice. There were seven transfer groups, i.e., immunized mouse serum, spleen cells, serum and spleen cells, normal mouse serum, spleen cells, serum and spleen cells, and control group. Three days later, BALB/c mice were inoculated with $1{\times}10^{4}$ trophozoites of N. fowleri intranasally. After infection, decreased mortality ana prolonged survival time of mice were noted in immunized Bloops compared with non.immuniBed control group. The groups Injected with immunized spleen cells or normal serum shewed lower moltality than that of controls bult showed no changes of Serum IgG level. The groups injected with immunized serum or normal spleen cells showed increased serum IgG level after immunization but hundred percent mortality was observed. Mother mice were ifnfnunised increperitqneeliy with $2~3{\times}10^{6}$ trephozoites of N. fowleri at the end of pregnancy and weaning Period. Soon after the delivery, Jitters born of non-immunszed mother were matched with immunized mother for feeding immune milk. After three weeks, the litters were infected with $1{\times}10^{4}$ trophozeites of N. fowleri or sacrificed for serum collection to measure the IgG levels. The results show that anti-JV. fowleri IgG from mother was transferred to litter through milk but this IgG did not inauence the mortality or survival time of the infected mice.

  • PDF

Numerical Analysis of Self-Supported Earth Retaining Wall with Stabilizing Piles (2열 자립식 흙막이 공법의 거동특성에 관한 수치해석적 연구)

  • Sim, Jae-Uk;Jeong, Sang-Seom;Lee, Jun-Hwan
    • Journal of the Korean Geotechnical Society
    • /
    • v.31 no.5
    • /
    • pp.35-46
    • /
    • 2015
  • In this study, the behavior of self-supported earth retaining wall with stabilizing piles was investigated by using a numerical study and field tests in urban excavations. This earth retaining wall can provide stable support against lateral earth pressures through its use of stabilizing piles that provide passive resistance to lateral earth pressures arising due to ground excavations. Field tests at two sites were performed to verify the performance of instrumented retaining wall with stabilizing piles. Furthermore, detailed 3D numerical analyses were conducted to provide insight into the in situ wall behavior. The 3D numerical methodology in the present study represents the behavior of the self-supported earth retaining wall with stabilizing piles. A number of 3D numerical analyses were carried out on the self-supported earth retaining wall with stabilizing piles to assess the results stemming from wide variations of influencing parameters such as the soil condition, the pile spacing, the distance between the front pile and the rear pile, and the pile embedded depth. Based on the results of the parametric study, the maximum horizontal displacement and the maximum bending moment significantly decreased when the retaining wall with stabilizing piles is used. Moreover, the horizontal displacement reduction effect of influencing parameters such as the pile spacing and the distance between the front pile and the rear pile is more sensitive in sandy soil, with a higher friction angle compared to clayey soil. In engineering practice, reducing the pile spacing and increasing the distance between the front pile and the rear pile can effectively improve the stability of the self-supported earth retaining wall with stabilizing piles.

Input Balun Design Method for CMOS Differential LNA (차동 저 잡음 증폭기의 입력 발룬 설계 최적화 기법)

  • Yoon, Jae-Hyuk
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.28 no.5
    • /
    • pp.366-372
    • /
    • 2017
  • In this paper, the analysis of baluns that are inevitably required to design a differential low noise amplifier, The balun converts a single signal input from the antenna into a differential signal, which serves as an input to the differential amplifier. In addition, it protects the circuit from ESD(Electrostatic Discharge) coming through the antenna and helps with input matching. However, in the case of a passive balun used in general, since the AC signal is transmitted through electromagnetic coupling formed between two metal lines, it not only has loss without gain but also has the greatest influence on the total noise figure of the receiving end. Therefore, the design of a balun in a low-noise amplifier is very important, and it is important to design a balun in consideration of line width, line spacing, winding, radius, and layout symmetry that are necessary. In this paper, the factors to be considered for improving the quality factor of balun are summarized, and the tendency of variation of resistance, inductance, and capacitance of the balun according to design element change is analyzed. Based on the analysis results, it is proved that the design of input balun allows the design of low noise, high gain differential amplifier with gain of 24 dB and noise figure of 2.51 dB.

Evaluation of the Stability of Quay Wall under the Earthquake and Tsunami (지진 및 지진해일파 작용하의 해안안벽의 안정성평가)

  • Lee, Kwang-Ho;Ha, Sun-Wook;Lee, Kui-Seop;Kim, Do-Sam;Kim, Tae-Hyung
    • Journal of the Korean Geotechnical Society
    • /
    • v.27 no.3
    • /
    • pp.41-54
    • /
    • 2011
  • The present study analyzes the stability of waterfront quay wall under the combined action of earthquake and tsunami. Adopting the limit equilibrium method, the stability of waterfront quay wall is checked for both the sliding and overturning. Forces due to tsunami are compared with the proposed formula and the 3-D one-field Model for immiscible TWO-Phase flows (TWOPM-3D). Variations of the stability of wall are also proposed by the parametric study including tsunami water height, horizontal seismic acceleration coefficient, internal friction angle of soil, friction angle between the wall and the soil and the pore water pressure ratio. The present study about the stability of wall is also compared with the case when earthquake and tsunami are not considered. As a result, the result of numerical analysis about the tsunami force is similar to that of proposed formula. When earthquake and tsunami are simultaneously considered, the stability of wall in passive case significantly decreases and tsunami forces in active case are affected as a resistance force on the wall and so the stability of wall increases.

Legal Interpretation on Management Power of Article 7 Section 1 of Security Business Act (경비업법 제7조 제1항 "관리권 범위"에 대한 법적 해석)

  • Lee, Jong-Hwan;Lee, Min-Hyung
    • Korean Security Journal
    • /
    • no.26
    • /
    • pp.59-87
    • /
    • 2011
  • Security Business Act of Korea is leaning toward the aspect of regulation, while it provides few provisions about vesting the authority, which causes problems such as legal loopholes to be raised in regard to the rules about authorizing the private security personnel to perform security affairs properly and defining the limit of power. So, it should be done to interpret the law in order to draw the legal basis of empowering as well as to set limits of the authority of security main agent, and Article 7, Section 1, of Security Business Act is the very provision that involves the legal basis. In the scope of 'Management Power', the statutory authority in the first clause of Article 7 of Security Business Act, the security personnel can use force for self-defense, defense of others and property, and prevention of crimes. In addition, the powers of interrogation, access control, and eviction notice are involved in its scope. The private security personnel as the occupation assistant can take precautions and if the infringement on the benefit and protection of the law is imminent or done, he or she can use force within the limits of the passive resistance and the means of defiance on the basis of 'Management Power'. The private security personnel, however, can exercise the force only if the necessary conditions of legal defense, emergency evacuation, and legitimate act of criminal law are fulfilled.

  • PDF

The study on corrosion of the inner area of closed box-girder for unpainted weathering steel bridges (무도장 내후성 강 교량의 밀폐형 박스거더 내부의 부식에 대한 고찰)

  • Ma, Seung-Hwan;Noh, Young-Tai;Jang, Gun-Ik
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.16 no.4
    • /
    • pp.2391-2400
    • /
    • 2015
  • Weather proof steels are used for steel bridges due to its high corrosion resistance under atmospheric conditions. However, instead of forming stabilized rust layers, general rust occurs on weather proof steels under high humidity condition close to seawater or shady places. In Japan, therefore, they perform rust stabilization treatment instead of unpainted treatment due to severe atmospheric conditions. However, most of domestic weather proof steels were constructed unpainted in the form of closed box-girder, which makes the periodical repetition of dry and wet hard to occur. For the steel bridges constructed on the Han river, the evaporation of water, dew condensation due to temperature change, and stagnant water due to rain affect harmfully on the formation of passive film on weather proof steels. Thus, in this research, in order to analyze corrosion properties inside the closed box-girder for the unpainted weather proof steel bridge in the waterworks safety zone, multiple ways of analysis such as observation with eyes, cellophane-tape test, steel thickness measurement, surface corrosion potential measurement, electron microscope analysis, and X-ray diffraction analysis of the rust were performed. As a result, unstable rust layer was observed inside the closed box-girder, and severe corrosion was observed on the top and bottom of the flanges due to the effects of stagnant water caused by rain, dew condensation, and de-icing materials.

The Effectiveness of the Direct and Indirect Contract-Relax Technique in PNF (고유수용성신경근촉진법(PNF) 수축-이완 기법의 직접적 간접적 방법의 비교)

  • Kim, Nan-Young;Kim, Eun-Hye;Kim, Hyeon-Ji;Kim, Hee-On;Park, Sun-U;Park, Seul-Gi;Yu, Seung-Yong;Yu, Ji-Yeon;Lee, Hyun-Ok
    • PNF and Movement
    • /
    • v.14 no.1
    • /
    • pp.7-14
    • /
    • 2016
  • Purpose: The purpose of this study was to determine the effectiveness of direct and indirect methods of contract-relax techniques in proprioceptive neuromuscular facilitation (PNF) stretching for improving hamstring flexibility. Methods: Twenty-six subjects were randomly assigned to either a direct contract-relax technique group or an indirect contract-relax group. For each group, stretching intervention was performed three times a week for a period of two weeks, with 12 seconds for the contraction and 10 seconds for the relaxation per once. The direct contract-relax technique for hamstring flexibility was applied by asking the subject to press down on the shoulder of the trainer in the straight leg-raising position. Conversely, the indirect contract-relax technique was performed by raising the leg with resistance. To facilitate a comparative analysis of hamstring flexibility between the two groups before and after the stretching intervention, two-way repeated ANOVA was performed. Hamstring flexibility for each group was measured using a passive straight leg-raising test. Results: The results showed significant improvement in hamstring flexibility for all subjects in the two groups. However, there was no significant difference between the groups. Conclusion: In conclusion, both direct and indirect contract-relax techniques are confirmed to be useful for improving hamstring flexibility. The choice of suitable technique has to be made individually according to the condition of each subject.

Dynamic Structural Response Characteristics of Stiffened Blast Wall under Explosion Loads (폭발 하중을 받는 보강된 방폭벽의 동적 구조 응답 특성에 관한 연구)

  • Kim, Sang Jin;Sohn, Jung Min;Lee, Jong Chan;Li, Chun Bao;Seong, Dong Jin;Paik, Jeom Kee
    • Journal of the Society of Naval Architects of Korea
    • /
    • v.51 no.5
    • /
    • pp.380-387
    • /
    • 2014
  • Piper Alpha disaster drew attention to the damage likely to arise from explosions and fires on an offshore platform. And great concerns have been increased to prevent these hazards. Blast wall is one of the passive safety systems; it plays a key part of minimizing the consequences. However, a buckling due to explosion loads is a factor which can reduce the strength of blast wall. The buckling often occurs between web and flange at the center of blast wall. This study aims to find a solution for reinforcing its strength by installing a flat plate at the spot where the buckling occurs. First of all, ANSYS finite element method is adopted to numerically compute the structural resistance characteristic of blast wall by using a quasi-static approach. Sequentially, the impact response characteristics of blast wall are investigated the effect on thickness of flat plate by using ANSYS/LS-DYNA. Finally, pressure-impulse diagrams (P-I diagram) are presented to permit easy assessment of structural response characteristics of stiffened blast wall. In this study, effective use is made to increase structural intensity. of blast wall and acquired important insights have been documented.