• Title/Summary/Keyword: Particle separation

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Leukocyte Segmentation using Saliency Map and Stepwise Region-merging (중요도 맵과 단계적 영역병합을 이용한 백혈구 분할)

  • Gim, Ja-Won;Ko, Byoung-Chul;Nam, Jae-Yeal
    • The KIPS Transactions:PartB
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    • v.17B no.3
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    • pp.239-248
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    • 2010
  • Leukocyte in blood smear image provides significant information to doctors for diagnosis of patient health status. Therefore, it is necessary step to separate leukocyte from blood smear image among various blood cells for early disease prediction. In this paper, we present a saliency map and stepwise region merging based leukocyte segmentation method. Since leukocyte region has salient color and texture, we create a saliency map using these feature map. Saliency map is used for sub-image separation. Then, clustering is performed on each sub-image using mean-shift. After mean-shift is applied, stepwise region-merging is applied to particle clusters to obtain final leukocyte nucleus. The experimental results show that our system can indeed improve segmentation performance compared to previous researches with average accuracy rate of 71%.

PIV Measurement of Viscous Flow Field in the Wake of Transom Stern (PIV기법을 이용한 트랜섬 선미 후류 점성유동장 계측)

  • Lee, Gyoung-Woo;Gim, Ok-Sok
    • Journal of Navigation and Port Research
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    • v.35 no.10
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    • pp.805-810
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    • 2011
  • An experiment was carried out to figure out the instantaneous flow characteristics in the wake of the transom stern's 2-dimensional section by 2-frame grey level cross correlation PIV method at $Re=3.5{\times}103$, $Re=7.0{\times}103$. The stern angles of models were learning at $45^{\circ}$(Model "A"), $90^{\circ}$(Model "B") and $135^{\circ}$(Model "C") respectively based on the survey results of real ships. The depth of wetted surface is 40mm from free surface. As Reynolds number increases, vortices increase in volume and move their way to the downstream. Flow separation appeared at the end of model's bottom.

Control Effect of Hydro-kinetic Force of a Special Rudder attached Flap (플랩이 부착된 특수타의 동유체력 제어효과에 관한 연구)

  • Gim, Ok-Sok;Lee, Gyoung-Woo;Ahn, Young-Kyu
    • Journal of Navigation and Port Research
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    • v.32 no.10
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    • pp.771-776
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    • 2008
  • The main purpose in having a control surface on a ship is to control the motion of it. It is the important element to determine the maneuvering characteristics of the ship. In this paper, the measured results has been compared with each other to predict the performance characteristics of flapped rudder's 2-dimensional section at $Re=3.0{\times}10^4$ using 2-frame grey level cross correlation PIV method. The side force of the rudder could be mainly improved by the lift force at 10 degrees angle of attack and the drag force at 20 degrees angle of attack. The separation point and boundary layer could be controlled by the change of the only flap's angle at 10 degrees angle of attack.

Method development and validation of spectrophotometric and RP-HPLC methods for simultaneous estimation of spironolactone and furosemide in bulk and combined tablet dosage forms

  • Chavan, Rohankumar R.;Bhinge, Somnath D.;Bhutkar, Mangesh A.;Randive, Dheeraj S.;Salunkhe, Vijay R.
    • Analytical Science and Technology
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    • v.34 no.5
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    • pp.212-224
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    • 2021
  • The intent of the present work was to develop a simple, sensitive, accurate, precise, rapid and economical UV- spectrophotometric and reverse phase high pressure liquid chromatographic method for the simultaneous estimation of Spironolactone and Furosemide in bulk and combined tablet dosage forms. UV-Spectrophotometry was carried out by simultaneous equation method using 0.02 M potassium dihydrogen phosphate buffer pH 3.5: Acetonitrile (50:50) v/v as a solvent. The linearity range was 2-14 ㎍ mL-1 for Spironolactone and Furosemide with a correlation coefficient > 0.99. The chromatographic separation was achieved on 250 mm × 4.6 mm, hypersil BDS C18 column with particle size 5 ㎛, by using an isocratic mixture of 0.02 M potassium dihydrogen phosphate buffer pH 3.5: Acetonitrile: tert butyl methyl ether (49:50:1) v/v/v as a solvent at a flow rate of 1 mL min-1 and UV detection was carried out at 254 nm. The retention time were observed to be 3.666 and 6.661 minutes for Furosemide and Spironolactone respectively. The two developed methods were validated according to the ICH guidelines for accuracy, precision, linearity, LOD, LOQ and were found to be within the limits. It can be concluded that these two methods could be successfully used for the simultaneous estimation of Spironolactone and Furosemide in bulk and combined tablet dosage forms.

OGLE-2017-BLG-1049: ANOTHER GIANT PLANET MICROLENSING EVENT

  • Kim, Yun Hak;Chung, Sun-Ju;Udalski, A.;Bond, Ian A.;Jung, Youn Kil;Gould, Andrew;Albrow, Michael D.;Han, Cheongho;Hwang, Kyu-Ha;Ryu, Yoon-Hyun;Shin, In-Gu;Shvartzvald, Yossi;Yee, Jennifer C.;Zang, Weicheng;Cha, Sang-Mok;Kim, Dong-Jin;Kim, Hyoun-Woo;Kim, Seung-Lee;Lee, Chung-Uk;Lee, Dong-Joo
    • Journal of The Korean Astronomical Society
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    • v.53 no.6
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    • pp.161-168
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    • 2020
  • We report the discovery of a giant exoplanet in the microlensing event OGLE-2017-BLG-1049, with a planet-host star mass ratio of q = 9.53 ± 0.39 × 10-3 and a caustic crossing feature in Korea Microlensing Telescope Network (KMTNet) observations. The caustic crossing feature yields an angular Einstein radius of θE = 0.52 ± 0.11 mas. However, the microlens parallax is not measured because the time scale of the event, tE ≃ 29 days, is too short. Thus, we perform a Bayesian analysis to estimate physical quantities of the lens system. We find that the lens system has a star with mass Mh = 0.55+0.36-0.29 M⊙ hosting a giant planet with Mp = 5.53+3.62-2.87 MJup, at a distance of DL = 5.67+1.11-1.52 kpc. The projected star-planet separation is a⊥ = 3.92+1.10-1.32 au. This means that the planet is located beyond the snow line of the host. The relative lens-source proper motion is μrel ~ 7 mas yr-1, thus the lens and source will be separated from each other within 10 years. After this, it will be possible to measure the flux of the host star with 30 meter class telescopes and to determine its mass.

Preparation of Nanoflake Bi2MoO6 Photocatalyst Using CO(NH2)2 as Structure Orientation and Its Visible Light Degradation of Tetracycline Hydrochloride

  • Hu, Pengwei;Zheng, Dewen;Xian, Yuxi;Hu, Xianhai;Zhang, Qian;Wang, Shanyu;Li, Mingjun;Cheng, Congliang;Liu, Jin;Wang, Ping
    • Korean Journal of Materials Research
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    • v.31 no.6
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    • pp.325-330
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    • 2021
  • Bi2MoO6 (BMO) via the structure-directing role of CO(NH2)2 is successfully prepared via a facile solvothermal route. The structure, morphology, and photocatalytic performance of the nanoflake BMO are characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), fluorescence spectrum analysis (PL), UV-vis spectroscopy (UV-vis) and electrochemical test. SEM images show that the size of nanoflake BMO is about 50 ~ 200 nm. PL and electrochemical analysis show that the nanoflake BMO has a lower recombination rate of photogenerated carriers than particle BMO. The photocatalytic degradation of tetracycline hydrochloride (TC) by nanoflake BMO under visible light is investigated. The results show that the nanoflake BMO-3 has the highest degradation efficiency under visible light, and the degradation efficiency reached 75 % within 120 min, attributed to the unique hierarchical structure, efficient carrier separation and sufficient free radicals to generate active center synergies. The photocatalytic reaction mechanism of TC degradation on the nanoflake BMO is proposed.

Extraction of dietary fibers from cassava pulp and cassava distiller's dried grains and assessment of their components using Fourier transform infrared spectroscopy to determine their further use as a functional feed in animal diets

  • Okrathok, Supattra;Thumanu, Kanjana;Pukkung, Chayanan;Molee, Wittawat;Khempaka, Sutisa
    • Animal Bioscience
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    • v.35 no.7
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    • pp.1048-1058
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    • 2022
  • Objective: The present study was to investigate the extraction conditions of dietary fiber from dried cassava pulp (DCP) and cassava distiller's dried grains (CDG) under different NaOH concentrations, and the Fourier transform infrared (FTIR) was used to determine the dietary fiber components. Methods: The dried samples (DCP and CDG) were treated with various concentrations of NaOH at levels of 2%, 4%, 6%, and 8% using a completely randomized design with 4 replications of each. After extraction, the residual DCP and CDG dietary fiber were dried in a hot air oven at 55℃ to 60℃. Finally, the oven dried extracted dietary fiber was powdered to a particle size of 1 mm. Both extracted dietary fibers were analyzed for their chemical composition and determined by FTIR. Results: The DCP and CDG treated with NaOH linearly or quadratically or cubically (p<0.05) increased the total dietary fiber (TDF) and insoluble fiber (IDF). The optimal conditions for extracting dietary fiber from DCP and CDG were under treatment with 6% and 4% NaOH, respectively, as these conditions yielded the highest TDF and IDF contents. These results were associated with the FTIR spectra integration for a semi-quantitative analysis, which obtained the highest cellulose content in dietary fiber extracted from DCP and CDG with 6% and 4% NaOH solution, respectively. The principal component analysis illustrated clear separation of spectral distribution in cassava pulp extracted dietary fiber (DFCP) and cassava distiller's dried grains extracted dietary fiber (DFCDG) when treated with 6% and 4% NaOH, respectively. Conclusion: The optimal conditions for the extraction of dietary fiber from DCP and CDG were treatment with 6% and 4% NaOH solution, respectively. In addition, FTIR spectroscopy proved itself to be a powerful tool for fiber identification.

Mineralogical Characteristics and Fundamental Study of Flotation for Molybdenum Ore (몰리브덴광의 광물학적 특성 및 부선 기초연구)

  • Oyunbileg Purev;Hyun Soo Kim;Chul-Hyun Park
    • Resources Recycling
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    • v.31 no.6
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    • pp.73-80
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    • 2022
  • This study investigated the mineralogical characteristics and basic flotation properties of domestic molybdenum ores. The source mineral of molybdenum was identified as molybdenite, and the main gangue minerals in the raw ore were silicate minerals. Copper, lead, and zinc were also found in trace amounts. Based on the results of basic flotation properties, molybdenite's zeta potential showed negative charges in all pH ranges. The contact angle of molybdenite increased with pH, reaching a maximum of 74° at pH 9. In optimal conditions, the grade and recovery of the concentrate by unit flotation were MoS2 82.4% and 92.04%, respectively. Further investigation of the impurities in the concentrate revealed a sulfide mineral with surface characteristics similar to molybdenite and silicate minerals combined with molybdenite, which may degrade the quality of the concentrate. To improve the concentrate quality, we intend to control silicate minerals through regrinding and liberation and use column flotation to improve fine particle separation efficiency.

Particle-motion-tracking Algorithm for the Evaluation of the Multi-physical Properties of Single Nanoparticles (단일 나노입자의 다중 물리량의 평가를 위한 입자 모션 트랙킹 알고리즘)

  • Park, Yeeun;Kang, Geeyoon;Park, Minsu;Noh, Hyowoong;Park, Hongsik
    • Journal of Sensor Science and Technology
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    • v.31 no.3
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    • pp.175-179
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    • 2022
  • The physical properties of biomaterials are important for their isolation and separation from body fluids. In particular, the precise evaluation of the multi-physical properties of single biomolecules is essential in that the correlation between physical and biological properties of specific biomolecule. However, the majority of scientific equipment, can only determine specific-physical properties of single nanoparticles, making the evaluation of the multi-physical properties difficult. The improvement of analytical techniques for the evaluation of multi-physical properties is therefore required in various research fields. In this study, we developed a motion-tracking algorithm to evaluate the multi-physical properties of single-nanoparticles by analyzing their behavior. We observed the Brownian motion and electric-field-induced drift of fluorescent nanoparticles injected in a microfluidic chip with two electrodes using confocal microscopy. The proposed algorithm is able to determine the size of the nanoparticles by i) removing the background noise from images, ii) tracking the motion of nanoparticles using the circular-Hough transform, iii) extracting the mean squared displacement (MSD) of the tracked nanoparticles, and iv) applying the MSD to the Stokes-Einstein equation. We compared the evaluated size of the nanoparticles with the size measured by SEM. We also determined the zeta-potential and surface-charge density of the nanoparticles using the extracted electrophoretic velocity and the Helmholtz-Smoluchowski equation. The proposed motion-tracking algorithm could be employed in various fields related to biomaterial analysis, such as exosome analysis.

Characteristics of Particle Size Distribution in the Organic Fraction of Municipal Solid Waste by the Reaction of Super-heated Steam (과열증기 반응에 의한 생활폐기물 유기성분 입도특성)

  • Jang, Ha-Na;Min, Tai-Jin;Roh, Seon-Ah;Kim, Woo-Hyun;Sung, Hyun-Je;Park, Seong-Bum
    • Journal of the Korea Organic Resources Recycling Association
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    • v.18 no.4
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    • pp.64-68
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    • 2010
  • Recently, MBT(Mechanical Biological Treatment) facilities were built up and operated to separate and recycle MSW(Municipal Solid Wastes)in South Korea. However, the size distribution of MSW is very rough, and it is causing operation problem because MSW would be crushed in undersize diameter by mechanical equipment before feeding each seperation process. Also, the organic material should be pre-seperated to reuse recycle material in MSW. In this research, the reactor of 1ton/batch using the super-heated steam was tested to present the separation efficiency and the size distribution of MSW by experimental factors.