• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.259-259
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• 2004

Porcine sperm extract (PSE) supporting Ca/sup 2+/ osillation was microinjected into the in vitro matured porcine oocytes. In the presence of the capacitative Ca/sup 2+/ entry mechanism which can activate MII oocytes, preparation methods of sperm extraction were studied by many researchers. Such as freeze-thaw cycle, homogenation, sonication of boar sperm was used for certification of their activity of calcium signals. (omitted)

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.39-40
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• 2005

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.138-138
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• 2005

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.138-138
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• 2005

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.39-40
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• 2005

• 한국응용곤충학회지
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• 제30권2호
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• pp.101-105
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• 1991

멸구류의 난기생봉인 Anagrus incarnatus Haliday의 기주탐색, 고매행동, 산란습성 그리고 생색형태 조사한 결과는 다음과 같다. 난괴가 있는 엽초까지 도달하는데 소요되는 시간은 평균188.5초였고 기주에 도달후 안테나의 drumming시간은 17.5초, tapping 72.4초, 삽입하여 산란하는데 206.7초 걸렸으며 교미 시간은 16~21초 정도였다. 본 종은 양성생식과 단위생식을 겸하며 교미시의 암수 비율은 약 3:1이었고 자성단위 생식형이었다.

• Journal of Species Research
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• 제4권1호
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• pp.1-22
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• 2015

Surveys of easily accessible or moderately remote South Korean mountains has revealed several common exotic and early species-complexes [Amynthas corticis (Kinberg, 1867) with A. diffringens (Baird, 1869), A. gracilis (Kinberg, 1867) and Metaphire californica (Kinberg, 1867)], plus an unexpected number of new native taxa. Megascolecid Metaphire muuido sp. nov. and lumbricid Eisenia muuido sp. nov. are newly described from Muuido Island, Incheon. Montane taxa are parthenogenetic Amynthas tokioensis oculo sub-sp. nov. that lacks male pores but is yet comparable to both Amynthas tokioensis (Beddard, 1892) and Metaphire soulensis (Kobayashi, 1938) with its possible new synonym A. chiakensis Hong & James, 2013. Apparently unique sympatric taxa are Amynthas bangtaesan bangtaesan and A. b. confinius sup-spp. nov., Amynthas centurio sp. nov., Amynthas punicans sp. nov., Amynthas seoraksan and A. seoraksan iti sub-spp. nov. These are newly described and their DNA COI gene barcodes, where obtainable, are presented in a phylogram with outgroup Acanthodrilidae Microscolex dubius (Fletcher, 1887) from Lake Biwa Japan being a new exotic record for Asia.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.333-337
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• 2011

A new and a unique Brachionus rotifer was found in Hwajinpo coastal lagoon in Gangwon Province, South Korea. This Brachionus certainly originated from the wild rather than from aquaculture stations because Hwajinpo coastal lagoon has been under rigorous control as a military protected area and therefore could not have been contaminated by aquaculture stations. The new strain was identified as Brachionus rotundiformis based upon its morphological characteristics. The parthenogenetic female of this new rotifer strain typically shows characters similar to those of B. rotundiformis, such as the pot shape of the body, rounded dorsal plate compared with flattened ventral plate, elliptical mictic egg, four frontal spines, six pointed occipital spines, non-nodal foot, two toes, trophi typical of the Brachionus genus with five uncus plates resembling comb teeth, one wide symmetrical manubrium and ramus, and no stiffened spine as is seen in freshwater Brachionus rotifers. Moreover, its lorica was rather small in size compared with other common rotifer strains that serve as live-food organisms (Guam, Thai, and Bali strains). This new and unique Korean brackish rotifer, a B. rotundiformis strain, was therefore named the National Fisheries Research and Development Institute (NFRDI) $N^{\circ}1$ rotifer strain.

• 한국수정란이식학회지
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• 제27권3호
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• pp.171-174
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• 2012

Various small molecules can be used to control major signaling pathways to enhance stemness and inhibit differentiation in murine embryonic stem cell (mESC) culture. Small molecules inhibiting the fibroblast growth factor (FGF)/ERK pathway can preserve pluripotent cells from stimulation of differentiation. In this study, we aimed to evaluate the effect of pluripotin (SC-1), an inhibitor of the FGF/ERK pathway, on the colony formation of outgrowing presumptive mESCs. After plating the zona pellucida-free blastocyst on the feeder layer, attached cell clumps was cultured with SC-1 until the endpoint of the experiment at passage 10. In this experiment, when the number of colonies was counted at passage 3, SC-1-treated group showed 3.4 fold more mESC colonies when compared with control group. However, after passage 4, there was no stimulating effect of SC-1 on the colony formation. In conclusion, SC-1 treatment can be used to promote mESC generation by increasing the number of early mESC colonies.

• International Journal of Industrial Entomology and Biomaterials
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• 제13권2호
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• pp.63-72
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• 2006

Parthenogenesis, the development of unfertilized ovum opens new perspectives in silkworm breeding in the development of homozygous breeds. In order to improve induction of artificial parthenogenesis in the excised unfertilized eggs of different breeds of the silkworm, Bombyx mori L., a new method was devised and the results were compared with the routine method. General and specific combining abilities and hybrid vigour of newly developed bivoltine breeds were analyzed utilizing bivoltine breeds viz., $CSR_2,\;CSR_4,\;CSR_{17}\;and\;NB_4D_2$ Estimation of GCA revealed superiority of the breeds, $DNB_1$ for eight characters followed by $DNB_4$ for five characters. Among the testers, $CSR_2$ was found good general combiner for seven characters followed by $CSR_{17}$ for four characters. A great deal of variations was observed among the hybrids studied. Five hybrids namely, $DNB_1{\times}CSR_2,\;DNB_4{\times}CSR_4,\;DNB_4{\times}NB_4D_2,\;DNB_6{\times}CSR_2\;and\;DNB_7{\times}CSR_2$ showed significant SCA effects for 5-6 characters. The hybrid, $DNB_4{\times}CSR_4$ showed its superiority by expressing significant hybrid vigour over BPV for 7 characters. Majority of the hybrids exhibited significant hybrid vigour for survival rate, yield/10,000 larvae by weight, cocoon weight, cocoon shell weight, filament length and denier.