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Efficient Production of Parthenogenetic Murine Embryonic Stem Cells by the Treatment of Pluripotin (SC-1)  

Kang, Hoin (Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry)
Roh, Sangho (Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry)
Publication Information
Journal of Embryo Transfer / v.27, no.3, 2012 , pp. 171-174 More about this Journal
Abstract
Various small molecules can be used to control major signaling pathways to enhance stemness and inhibit differentiation in murine embryonic stem cell (mESC) culture. Small molecules inhibiting the fibroblast growth factor (FGF)/ERK pathway can preserve pluripotent cells from stimulation of differentiation. In this study, we aimed to evaluate the effect of pluripotin (SC-1), an inhibitor of the FGF/ERK pathway, on the colony formation of outgrowing presumptive mESCs. After plating the zona pellucida-free blastocyst on the feeder layer, attached cell clumps was cultured with SC-1 until the endpoint of the experiment at passage 10. In this experiment, when the number of colonies was counted at passage 3, SC-1-treated group showed 3.4 fold more mESC colonies when compared with control group. However, after passage 4, there was no stimulating effect of SC-1 on the colony formation. In conclusion, SC-1 treatment can be used to promote mESC generation by increasing the number of early mESC colonies.
Keywords
pluripotin (SC-1); small molecule; embryonic stem cells; mouse; FGF/ERK pathway;
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