An Improved Method of Parthenogenetic Development and Analysis of Combining Ability in Bivoltine Breeds of the Silkworm, Bombyx mori L.

  • Published : 2006.12.31

Abstract

Parthenogenesis, the development of unfertilized ovum opens new perspectives in silkworm breeding in the development of homozygous breeds. In order to improve induction of artificial parthenogenesis in the excised unfertilized eggs of different breeds of the silkworm, Bombyx mori L., a new method was devised and the results were compared with the routine method. General and specific combining abilities and hybrid vigour of newly developed bivoltine breeds were analyzed utilizing bivoltine breeds viz., $CSR_2,\;CSR_4,\;CSR_{17}\;and\;NB_4D_2$ Estimation of GCA revealed superiority of the breeds, $DNB_1$ for eight characters followed by $DNB_4$ for five characters. Among the testers, $CSR_2$ was found good general combiner for seven characters followed by $CSR_{17}$ for four characters. A great deal of variations was observed among the hybrids studied. Five hybrids namely, $DNB_1{\times}CSR_2,\;DNB_4{\times}CSR_4,\;DNB_4{\times}NB_4D_2,\;DNB_6{\times}CSR_2\;and\;DNB_7{\times}CSR_2$ showed significant SCA effects for 5-6 characters. The hybrid, $DNB_4{\times}CSR_4$ showed its superiority by expressing significant hybrid vigour over BPV for 7 characters. Majority of the hybrids exhibited significant hybrid vigour for survival rate, yield/10,000 larvae by weight, cocoon weight, cocoon shell weight, filament length and denier.

Keywords

References

  1. Astaurov, B. L. (1940) Artificial parthenogenesis in the silk­worm (Bombyx mori L.) (Experimental study). Accademia delle Scienze Dell'urss. pp. 221-240
  2. Bhargava, S. K., M. K. Majumdar and R. K. Datta (1995) Combining ability of seven silk technological characters in the mulberry silkworm, Bombyx mori. Bull. Zool. 62, 359-­362 https://doi.org/10.1080/11250009509356089
  3. Datta, R. K. (1984) Improvement of silkworm races (Bombyx mori L.) in India. Serieologia 24, 393-415
  4. Datta, R. K., D. R. Rao, K. P. Jayaswal, V. Premalatha, Ravin­dra Singh and B. K. Kariappa (2001) Heterosis in relation to combining ability in multivoltine and bivoltine strains of silkworm, Bombyx mori L. Indian J. Seric. 40, 1-6
  5. Eid, M. A. A., S. A. S. EI-Maasarawy, A. Hosny and U. M. M. Ghazy (2005) Combining ability estimates in the silkworm, Bombyx mori L for larval and cocoon traits. Serieologia 45, 127-132
  6. Fonseca, S. and F. L. Patterson (1968) Hybrid vigour in seven parent diallel crosses in common winter wheat. Crop Sci. 6, 382-383 https://doi.org/10.2135/cropsci1966.0011183X000600040032x
  7. Hirokawa, M. (1990) Selection of the high parthenogenetic lines and examinations of their practical characters in com­mercial F1 hybrid races of the silkworm, Bombyx mori. Bull. Fukushima Serie. Exp. Stn. 24, 1-6
  8. Hirokawa, M. (1995) Studies on sex control in the silkworm, Bombyx mori. Bull. Fukushima Serie. Exp. Stn. 28, 1-104
  9. Jolly, M. S. (1983) Silkworm breeding in India. In: Silkworm genetics and breeding. Natl. Semin. Silk Res. Dev. Bangalore pp. 1-11
  10. Kempthome, O. (1957) An introduction to genetic statistics. pp. 208-341, John Wiley and Sons Inc., London
  11. Kumar, P. R. Bhutia and M. M. Ahsan (1994) Combining abil­ity analysis for filament length ans some quantitative traits in bivoltine mulberry silkworm (Bombyx mori L.). Indian J. Genet. 54, 253-257
  12. Nagaraju, J., Raje, Urs and R. K. Datta (1996) Crossbreeding and heterosis in the silkworm, Bombyx mori, a review. Serieologia 36, 1-21
  13. Ohkuma, T. (1971) Studies on the mechanism of hybrid vigour by means of artificial parthenogenesis in the silkworm, Bom­byx mori L. J. Serie. Sci. Jpn. 40, 422-430
  14. Pershad, G. D., R. K. Datta, S. K. Bhargava, H. Vijayakumar and M. S. Jolly (1986) Combining ability analysis in multi­voltine races of silkworm, Bombyx mari L. Sericologia 36, 307-315
  15. Rajalakshmi, E., T. P. S., Chauhan, V. Thiagarajan, V. Laksh­manan and C. K. Kamble (1997) Line ${\times}$ tester analysis of combining ability in new genotypes of bivoltine silkworm (Bombyx mori). Indian J. Agrie. Sci. 67, 287-290
  16. Ram, D., G. Kalloo and M. Singh (1999) Combining ability of quantitative characters in bittergourd (Monardiea eharontia). Indian J. Agrie. Sci. 69, 122-125
  17. Rao, D. R., Ravindra Singh, V. Premalatha, B. K. Kariappa, M. Rekha and K. P. Jayaswal (2002) Manifestation of hybrid vigour and combining ability in polyvoltine ${\times}$ bivoltine hybrids of silkworm, Bombyx mori L. Int. J. Indust. Entomol. 4, 23­-30
  18. Ravindra Singh, D. R. Rao, B. K. Kariappa, V. Premalatha and S. B. Dandin (2003) Studies on analysis of combining ability in the mulberry silkworm, Bombyx mori L. Int. J. Indust. Entomol. 6, 107-113
  19. Ravindra Singh, D. R. Rao, V. Premalatha, B. K. Kariappa, K. P. Jayaswal and R. K. Datta (2001) Evaluation of combining ability in hybrids between low, medium and high cocoon weight polyvoltine and bivoltine breeds of silkworm, Bam­byx mori L. Serieologia 41, 57-64
  20. Ravindra Singh, G. V. Kalpana, P. Sudhakara Rao, M. M. Ahsan, R. K. Datta and M. Rekha (2000) Studies on combin­ing ability and heterosis in the silkworm, Bombyx mori L. Indian J. Seric. 39, 43-48
  21. Ravindra Singh, K. P. Jayaswal and B. Saratchandra (1994) Parthenogenetic development of ovarian eggs in some breeds of silkworm, Bombyx mori L. Entomon. 19, 57-62
  22. Ravindra Singh, S. D. Sharma, D. R. Rao, K. Chandrashekha­ran, H. K. Basavaraja and B. K. Kariappa (2005) Line ${\times}$ tester and heterosis analysis in the silkworm, Bombyx mori L. Indian J. Seric. 44, 92-99
  23. Satenahalli, S. B., R. Govindan and J. V. Goud (1989) Genetic analysis of some quantitative traits by diallel cross in silk­worm, Bombyx mori L. Sericologia 29, 333-342
  24. Strunnikov, V. A. (1983) Control of silkworm reproduction, development and sex. Mir Publishers, Moscow
  25. Strunnikov, V. A. (1986) Nature of heterosis and combining ability in the silkworm. Theor. Appl. Genet. 72, 503-512 https://doi.org/10.1007/BF00289532
  26. Strunnikov, V. A., S. S. Lezhenko and E. A. Stepanova (1982) Genetical identical copies of the mulberry silkworm. Theor. Appl. Genet. 63, 307-315 https://doi.org/10.1007/BF00303901
  27. Subba Rao, G. and V. Sahai (1989) Combining ability and het­erosis studies in bivoltine strains of silkworm, Bombyx mori L. Uttar Pradesh J. Zool. 9, 150-164
  28. Takei, R., M. Nakagaki, R. Kodaira and E. Nagashima (1990) Factor analysis on the parthenogenetic development of ova­rian eggs in the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). Appl. Entomol. Zool. 25, 43-48 https://doi.org/10.1303/aez.25.43