• 대한미생물학회지
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• 제35권3호
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• pp.203-214
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• 2000

Porphyromonas gingivalis has been implicated in periodontal diseases. Accumulating evidence suggests that cardiovascular disease is the most prevalent medical problem in patients with periodontal diseases. In order to check the possibility that P. gingivalis is involved in coronary heart disease, the present study was performed to observe P. gingivalis adherence and invasion of human coronary artery endothelial cells (HCAEC) and production of cytokines and growth factors by HCAEC upon P. gingivalis infection. $^3H$-labeled P. gingivalis 381 was incubated with HCAEC for 90 min. The radioactivity of the washed HCAEC was a measure of the absorbed (adhering and invading) P. gingivalis. The absorption radioactivity of the HCAEC infected by P. gingivalis was determined to be 59.58% of the input bacterial cells. In contrast, the absorption radioactivity of the cells infected by S. gordonii Challis which was employed as a control was negligible (0.59%). DPG3, a P. gingivalis mutant defective of fimbriae, appeared to be impaired to some extent in capability of adherence/invasion as compared to that of the parental strain 381, showing 43.04% of the absorption radioactivity. The absorption radioactivity of the HCAEC infected by P. gingivalis 381 in the presence of excessive fimbriae at the concentrations of $50\;{\mu}g$ and $100\;{\mu}g/ml$ was 57.27 and 45.44%, respectively. Invasion of HCAEC by P. gingivalis 381 was observed by an antibiotic (metronidazole) protection assay and transmission electron microscopy (TEM). In the antibiotic protection assay, invasion by the bacterium was measured to be 0.73, 1.09, and 1.51% of the input bacterial cells after incubation for 30, 60, and 90 min, respectively. Invasion by DPG3 was shown to be 0.16% after 90-min incubation. In comparison of invasion efficiency at 90 min of the incubation, the invasion efficiency of DPG3 was 0.37% while that of its parental strain 381 was 2.54%. The immunoblot analysis revealed fimbriae of P. gingivalis did not interact with the surface of HCAEC. These results suggest that fimbriae are not the major contribution to the adherence of P. gingivalis to HCAEC but may be important in the invasion of HCAEC by the bacterium. The presence of cytochalasin D ($1\;{\mu}g/ml$) and staurosporine ($1\;{\mu}M$) reduced the invasion of HCAEC by P. gingivalis 381 by 78.86 and 53.76%, respectively, indicating that cytoskeletal rearrangement and protein kinase of HCAEC are essential for the invasion. Infection of P. gingivalis induced HCAEC to increase the production of TNF-${\alpha}$. by 60.6%. At 90 min of the incubation, the HCAEC infected with P. gingivalis cells was apparently atypical in the shape, showing loss of the nuclear membrane and subcellular organelles. The overall results suggest that P. gingivalis may cause coronary heart disease by adhering to and invading endothelial cells, and subsequently damaging the cells.

• 인간행동과 음악연구
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• 제20권1호
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• pp.99-115
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• 2023

COVID-19 팬데믹 이후 비대면 음악치료에 대한 논의가 지속되고 있는 시점에 비대면 치료 시 보호자의 참여 및 역할의 중요성을 고려해 본 연구에서는 발달장애 아동·청소년에 제공되고 있는 실시간 비대면 음악치료에서 보호자가 어떠한 경험을 했고, 자신의 역할에 대해 어떻게 인식하는지 알아보고자 하였다. 이를 위해 만 2-18세의 발달장애 아동·청소년(이하 아동) 자녀를 둔 보호자 32명을 대상으로 비대면 음악치료 경험과 이에 대한 인식 등의 68문항으로 구성된 설문조사를 온라인으로 실시하였다. 수집된 자료에 대한 기술통계를 실시하였고, 비대면 음악치료에서 보호자가 수행한 역할에 대한 만족도, 비대면 음악치료에서 경험한 심리적 부담감, 향후 비대면 음악치료 참여 의사 수준 간 상관관계를 Pearson's correlation을 통해 분석하였다. 분석 결과, 발달장애 아동 보호자들은 비대면 음악치료와 관련된 많은 정보를 가지고 있고, 비대면 음악치료의 혜택에 대해 인식하고 있는 것으로 나타났다. 또한 자신이 참여적 보조 및 중재적 보조 역할을 수행한 것에 대한 만족도가 높을수록 비대면 음악치료에서 가진 심리적 부담감이 낮은 것으로 나타났고, 비대면과 대면 음악치료의 이점을 다르게 인식하고 있음을 확인할 수 있었다. 이러한 결과는 포스트 코로나 시대를 대비해 새로운 패러다임으로서의 실시간 비대면 음악치료가 장애아동과 장애아동의 가족에게 의미하는 바에 대한 구체적인 자료를 제시할 수 있었으며, 이는 보호자들에게 보다 구체적이고 효과적으로 지원할 수 있는 방향에 대한 기초자료로 활용될 수 있을 것이다.

• 디지털융복합연구
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• 제11권2호
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• pp.375-383
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• 2013

본 연구는 기혼 유자녀 맞벌이 남성의 부성역할 수행 경험을 탐색하는 것으로 목적으로 한다. 이를 위해 심층면담과 문서를 활용하여 자료를 수집하였으며, 맞벌이 아버지 10명이 연구에 참여하였다. 자료수집은 2012년 9월부터 12월 사이에 이루어졌으며, 수집된 자료는 분석적 귀납에 근거한 지속적 비교분석방법을 사용해 분석하였다. 자료의 분석결과 참여자들은 바람직한 아버지 상에 대한 인식과 실제적인 역할 수행의 불일치, 생계부양자와 돌봄 제공자 역할 수행으로 인한 긴장, 그리고 자녀에 대한 헌신과 직업적 성취욕구 간의 갈등을 경험하고 있었으며, 이러한 갈등은 점진적으로 가족 의사소통과정에서의 소외라는 결과를 가져왔다. 이를 토대로 아버지의 적극적인 가족생활 참여를 지원하기 위한 사회적 인식 제고방안과 함께 가족, 노동시장, 지역사회, 그리고 정부 차원의 다각적인 접근과 제도의 운영을 제안하였다.

• 한국학교보건학회지
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• 제27권3호
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• pp.140-147
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• 2014

Purpose: This study aimed to identify the factors affecting on depression among female middle school students. Methods: A survey was conducted on 217 first and second grade female middle school students in C city. Participants' general characteristics, home environment, level of depression, paternal, maternal, peer attachment, school adjustment were measured by self-reporting questionnaire. With SPSS 18.0 program, measured variables were analyzed and compared using descriptive statistics, t-test, ANOVA, Scheff$\acute{e}$ post hoc test, and stepwise multiple regression analysis. Results: The level of depression among female middle school students were higher among students with lower economic status, second in birth order, and second grade in school. The level of depression was negatively associated with the level of paternal attachment (r=-.522), maternal attachment (r=-.487), peer attachment (r=-.376), relationship with teachers in school adjustment (r=-.274), relationship with friends in school adjustment (r=-.378), classroom behavior (r=-.450), school regulation (r=-.267). About 42% of depression among female middle school students were explained by paternal attachment, relationship with friends in school adjustment, and classroom behavior. Conclusion: Paternal attachment was identified as the most influencing factor on depression among female middle school students. Encouraging paternal involvement in the process of school adjustment might be a useful intervention to decrease depression among middle school girls.

• Human Ecology Research
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• 제53권2호
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• pp.169-177
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• 2015

The PRQ instrument is parental perspectives of the parent-child relationship. It measured parent-child dimensions that include attachment and involvement. Also, it provides additional information on parenting style, parenting stress and satisfaction with the child's school. This study tested reliability and validity of the Korean version of Parenting Relationship Questionnaire-Child (K-PRQ-C). The K-PRQ-C was administered to parents of 713 elementary school children including 362 boys and 351 girls. They were from 1st to 6th grade children who were recruited form 4 elementary schools in Korea. Reliability was tested through internal consistency. We conducted confirmatory factor analysis. Moreover, scale inter-correlation was conducted for validity test. Discriminant validity conducted through administration to the parents of clinical children with emotional and behavior disorders. Construct validity was also examined through Korean version of the Parenting Sense of Competence. The results of confirmatory factor analysis indicated that the similar factor structures exist for PRQ-C and K-PRQ-C. The results showed a high level of internal consistency as well as construct and discriminant validity. These results showed valid psychometric properties for the K-PRQ-C. The K-PRQ-C provides a reliable and valid means of gathering information about the parent-child relationship. We discussed Implications for the use of the K-PRQ-C as a clinical tool and as a research instrument.

• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.8595-8601
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• 2014

Background: miR-200a expression is frequently altered in numerous cancers. The aim of the present study was to determine the role of microRNA-200a in advanced ovarian carcinomas. Materials and Methods: We measured miR-200a expression in 72 matched normal ovarian tissues and advanced ovarian carcinomas, and also two ovarian carcinoma cell lines (SKOV3 and SKOV3.ip1 - the latter being more invasive and metastatic than the parental SKOV3) by stem-loop real-time RT-PCR based on TaqMan microRNA assay using U6 as a reference. Levels of miR-200a expression were compared by disease stage, tumor grade, histology, and lymph node involvement. To evaluate the role of microRNA-200a, cell proliferation and invasion of SKOV-3 and SKOV-3.ip1 were analyzed with miR-200a inhibitor/mimic transfected cells. Results: Of 72 paired samples, 65 cancer tissues overexpressed microRNA-200a greater than two fold in comparison with matched normal epithelium. Specifically, patients with lymph node metastasis showed significant elevation. The level correlated with clinicopathological features, including high tumor grade, late disease stage, most notably with lymph node metastasis, but not with tumor histology. In addition, SKOV-3.ip1 cells also overexpressed miR-200a compared with SKOV-3, and miR-200a inhibitor transfected SKOV-3.ip1 cells showed significant reduction in cellular proliferation and invasion, while a miR-200a mimic stimulated the opposite behavior. Conclusions: We provide definitive evidence that miR-200a is up-regulated in a significant proportion of advanced ovarian carcinomas, and that elevated miR-200a expression facilitates tumor progression. Our findings support the notion that miR-200a is an onco-microRNA for ovarian cancer, and elevation is a useful potential diagnostic indicator. This study also provides a solid basis for further functional analysis of miR-200a in advanced ovarian cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5725-5730
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• 2013

Cancer patients often suffer from local tumor recurrence after radiation therapy. Cell cycling, an intricate sequence of events which guarantees high genomic fidelity, has been suggested to affect DNA damage responses and eventual radioresistant characteristics of cancer cells. Here, we established a radioresistant lung cancer cell line, A549R, by exposing the parental A549 cells to repeated ${\gamma}$-ray irradiation with a total dose of 60 Gy. The radiosensitivity of A549 and A549R was confirmed using colony formation assays. We then focused on examination of the cell cycle distribution between A549 and A549R and found that the proportion of cells in the radioresistant S phase increased, whereas that in the radiosensitive G1 phase decreased. When A549 and A549R cells were exposed to 4 Gy irradiation the total differences in cell cycle redistribution suggested that G2-M cell cycle arrest plays a predominant role in mediating radioresistance. In order to further explore the possible mechanisms behind the cell cycle related radioresistance, we examined the expression of Cdc25 proteins which orchestrate cell cycle transitions. The results showed that expression of Cdc25c increased accompanied by the decrease of Cdc25a and we proposed that the quantity of Cdc25c, rather than activated Cdc25c or Cdc25a, determines the radioresistance of cells.

• 한국학교보건학회지
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• 제9권2호
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• pp.249-266
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• 1996

AIDS and the spectrum of Human Immunodeficiency Virus(HIV) infections present a monumental challenge to the health of the Korean public. In response to this special challenge, I think public education and voluntary behavior changes are the most effective measures to fight the spread of the disease. Adolescents represent a critical risk group for prevention and intervention programming. Research indicates sexually active adolescents, homosexual contact, illicit drug use are an gradually increasing. These characteristically adolescent risk-taking behaviors suggest the need for schools and communities to mobilize intervention strategies. Schools are highly efficient ways to reach a majority of young people in Korea with HIV prevention programs. These programs include substantial attention to sexual and drug use behaviors with the long term objective of a multidimensional school health program. Information resulting from risk behavior surveillance activities and guidance on school health curricula is particularly useful. What is needed for adolescents is a revamping of education to give students the critical thinking and analytic skills that allow them to apply knowledge, make decisions, and think independently. The best HIV preventive education provides young people with opportunities to learn and practice just those skills. In the early stages of HIV education were focused solely on information. Providing information is easy but unfortunately, behavior change is not that simple to activate. Information must be combined with values exploration and skilly building, including responsible decision making, negotiation, refusal, and critical thinking skills. The same knowledge, attitudes and skills needed for effective HIV prevention also prevent or reduce other risks, including other sexually transmitted diseases, unwanted pregnancies, and alcohol or other drug use. The role of other youth serving organizations in HIV prevention is also important: parental and youth involvement is needed; it's important to presidential and governament leadership is essential to prevention education; promote integrated adolescent programs, to enhance health and education sector collaboration; and of course, we need to expand research on adolescent health and engage the media in health promotion. Among these changes, a school-based systematic health education of AIDS is certainly one of the essentials.

• International Journal of Human Ecology
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• 제12권2호
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• pp.95-107
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• 2011

Recent intervention studies document that mother-child dyads with higher levels of Emotional Availability (EA) report fewer child behavior problems than dyads with lower EA. This study examines possible mechanisms that lead to this result by looking at the parent-child micro-system as a whole, with multi-dimensional relationships that include individual differences in the child's cognitive level, parental stress and parent-child interaction. A total 67 children ($1{\frac{1}{2}}$ to $5\;{\frac{1}{2}}$ years of age) and their mothers were videotaped during 30-min play interactions. Interactions were coded using the Emotional Availability (EA) Scales (Biringen, Robinson, & Emde, 1998). Mothers completed Parenting Stress Index-Short Form, Child Behavior Checklist/$1\;{\frac{1}{2}}$ - 5, and the Ages Stages Questionnaire. The findings showed that mothers with higher levels of parenting stress were more likely to be intrusive, hostile, insensitive, and had a tendency to do less structuring in play. The children of stressed and depressed mothers demonstrated less involvement and responsiveness towards their mothers. Children who have higher dyadic EA scores experienced fewer externalizing and internalizing problems. SEM analyses results showed a mediation effect of EA on the association between maternal psychological well-being and child behavior problems. Fewer deficits in child communication skills and problem solving skills that were related with lower parenting stress and depression were associated with higher maternal non-intrusiveness. Higher non-intrusiveness was related to less internalizing and externalizing problems that indicated the indirect effect of child cognitive competence. Possible interpretations and implications of the study findings are discussed.

• Journal of Yeungnam Medical Science
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• 제18권2호
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• pp.145-169
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• 2001

수술전, 후의 준비 및 처치를 적절히 할 수만 있다면 수술을 받은 후 귀가하는 외래수술은 특히 소아환자와 보호자로부터 환영받을 수 있는 안전하고 경제적인 방법이다. 대상환아의 선택시에는 수술의 종류와 소요시간 그리고 환아의 상태 등을 종합적으로 판단하여 수술후 합병증을 야기하지 않고, 빠른 회복이 가능한 대상을 선별토록 해야 하며 특히 소아의 연령에 따른 인지의 정도 및 심리적 다양성을 충분히 감안하여 마취유도시 까지는 정신외상을 주지 않도록 환아와의 rapport형성에 최대한의 배려를 해야 하며 수술후의 통증 및 회복시 야기될수 있는 부작용에 대하여는 가능한 한 예방이 바람직하나 초래된 경우에는 신속한 처치를 해주므로써 환아의 고통을 경감시켜 주도록 해야한다. 최근에 등장한 여러가지 이상적인 마취약제들과 아울러 합리적인 투여방법의 발달로 신속하고 부드러운 마취유도 및 수술이 끝난 후의 빠른 회복 그리고 부작용의 최소화가 가능하게 되었다. 그러나 국내의 의료현실상 아직은 이상적인 약제들을 의사의 판단에 따라 자유롭게 사용할 수 있는 여건이 되지 못하며 소아의 통증치료에 대하며 의료전이 소극적이며 수술후 관리에 대한 보호자들의 이해수준이 낮으므로 수술 당일 바로 환아를 병원에서 퇴원시키는 것은 상당한 위험성을 내포하고 있기 때문에 소아에서의 외래마취는 성인의 경우와는 달리 아직은 보편화되지 못하고 있는 실정이다.