• Journal of the Korean Institute of Landscape Architecture
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• v.22 no.4
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• pp.133-148
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• 1995

This study was carried out to provide behavioral data for its planning and management of civic garden through the evaluation of user's motivation & satisfaction. The civic gardens around Seoul were surveyed. The site are located in Kwangtan-myeon, Sudong-myeon, Wonsam-myeon, Nam-myeon, Buknae-myeon, and Jumdong-myeon, A total of 244 questionnaires were completed by mail questionnaires. The results are as follows: 1. 82 percentage of users are in thirties or fourties most of them have children who go to the elementary school. 50 percentage of users are just typical house wifes. 74 percentage of users graduated form university. 60 percentage of users live in lofty apartment buildings. 2. As a result from factor analysis, their motivations are categorized into four fator groups.: 'to experience nature', 'weekend recreation', 'nostalgia', 'to provide their old parent's sparetime'. And their satisfactions are categorized into eleven fator groups.: 'psychological/intellectual component', 'recreational components', 'instruction/management', 'facility'. 'vegetable cultivation', 'social contact', 'crowing', 'aesthetic component', 'family contact', 'the terms of lease', 'visition time'. 3. The user of civic garden can be divided into four user groups by their motivation. CLUSTER1 can represent the user group who have motivations for 'leisure, relaxation'. They are considerably satisfied with all other factor 'opportunity of meeting new person'. CLUSTER2 at the age of 41 to 50 have motivation for 'health, nostalgia'. CLUSTER3 at the age of 31 to 40 have motivation for 'harvest, experiencing nature'. CLUSTER4 at the age of more than 51 want to let their parents enjoy their sparetime. They are dissatisfied with accessibility, amount of cultivation area, crowding and overall farm management. 4. The regression analysis was employed with predicting the overall satisfaction. The results of regression analysis showed that 69% of total variances was explained by six variables: The most effective variable is 'whether visiting on weekend or weekdays', the visitors on weekdays are far more satisfied than weekend visitors because of traffic congestion, and crowding. The second source of satisfactions are 'psychological/intellectual components', they are satisfied with 'family contact', 'the terms of lease' and 'instruction in farming' are sources of satisfaction or dissatisfaction, and finally 'aesthetic landscape' is the source of satisfaction. The second most important variable is psychological one. Even though the civic gardens were not well equipped, they liked the atmosphere of rural life, refreshness, nostalgia, satisfaction from cultivation plants, and sense of achievement.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.8046-8056
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• 2015

The purpose of this study is the study of factors affecting the life satisfaction of disabled children parents. Survey data metropolitan area and Gyeonggi-do special schools, day shelter, Disabled Welfare, especially on the national branch in Korea Funny disabled parent meetings, through 389 branches were collected material wealth targeting parents with disabilities. Statistical analysis SPSS 19.0 program utilizes factor analysis, reliability analysis. And parents of children with disabilities and environmental factors are the demographic characteristics in order to analyze the impact on life satisfaction as a control variable, and the environmental factors as independent variables were performed hierarchical regression analysis. Results are crazy and the positive(+) influence a religious presence and the average monthly income of demographic characteristics in the satisfaction of life, environmental factors all had significant influence on life satisfaction. In other words, family support, disability, social networks, personal nature had a positive(+) influence on life satisfaction of disabled children parents, the relative influence of these factors include family support, disability, social networks, net of personality. It was in. The result is handicapped parents receive emotional support and help from family, the lower the degree of disability of the child, as participation in meetings or disability welfare various programs and handicapped parents, and the more it has a positive character increased satisfaction in life that can be interpreted.

• Journal of Elementary Mathematics Education in Korea
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• v.10 no.1
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• pp.89-106
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• 2006

The purposes of this study are to find out differences of the causes of mathematics anxiety in elementary school students according to the Grades and sex, and to find out which causes have more influence on achievement of mathematics and how much it is. For this purposes, the problems of this study are defined as follows: First, are there any differences in the influences of each super-causes and sub-causes of mathematics anxiety according to the Grades? Second, are there any differences in the influences of each super-causes and sub-causes of mathematics anxiety according to the sex? Third, what relation do the super-causes of mathematics anxiety have to the achievement of mathematics? The conclusions of this study are as follows: First, mathematics anxiety is much more affected by the internal cause like the cause of student attitude than the external cause like the causes of circumstance and the cause of teacher. Second. mathematics anxiety is much more affected by a direct experience like the causes of a shortage of time and the causes of student interest than indirect experience like the causes of teacher's authority and the causes of the application of daily life. Third, the causes of circumstance and parent's attitude. its sub-cause, have greater influence on the female group than on the male group. Fourth, in the middle Gradess, the female group is more affected by the cause of student attitude and the cause of circumstance than the male group, but in the higher Grades. the differences disappear and those two become common causes of anxiety. Fifth, As the students go up to the next Grades in school, the cause of teacher, the characteristic of the curriculum and the cause of prejudice have more influence on the mathematics anxiety. Sixth, the causes of teacher, the causes of mathematical curriculum and the causes of student attitude among super-causes of mathematics anxiety have a negative effect on the achievement of mathematics. But the causes of circumstance have a positive effect on it. And also, the causes of mathematical curriculum among super-causes is much related to the causes of teacher and the causes of circumstance.

• Journal of Embryo Transfer
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• v.29 no.3
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• pp.289-296
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• 2014

The pedigree information and race records of 1,000 m finishing time of Jeju race horses at KRA were used to study the effect of amount of pedigree information and parental misidentification on the estimates of genetic parameters. The modified data sets were made at the range of 2.5 to 25% parental misidentifications or loss of parental information of individuals with an increment of 2.5 percent. For each incremental level, 20 randomly replicated data sets were obtained and analyzed by single-trait analysis with a DF-REML(AI) algorithm. As the rate of misidentification increased or the amount of pedigree information decreased, the estimates of fraction of additive genetics variance component gradually decreased almost linearly (p<0.05), while the estimated fractions of error variance and permanent environmental variance components gradually increased for the finishing time. Regression coefficients of the percentage amount of both parents' information loss and incorrect pedigree information on additive genetic variances were -0.079 and -0.114, respectively (p<0.01). The estimate of heritability decreased by 0.92% for one percent loss of both parents' information and 1.39% for one percent increase of both parental misidentifications of progeny (p<0.01). For the consideration of probable incorrect and missing parent information of progeny in this early population of Jeju horses, the estimates of additive genetic parameters would be biased downward about ten percent. This results indicate that the amount of pedigree information loss and misidentification of progeny would severely affect estimates of genetic parameters and would reduce genetic gains for selection in Jeju horse population.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.2
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• pp.147-154
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• 1989

The experiments were carried out to compare the growth characteristics of excised seminal roots in Japonica type, Indica- Japonica hybrids and its parents. Based on the modified culture medium by Kawata et al., several levels of pH. sucrose and casamino acid were varied in the culture media. The excised root length at 27$^{\circ}C$ incubator was little different between Japonica type and Indica-Japonica hybrids, but at 30$^{\circ}C$ incubator the excised root elongation of Indica-Japonica hybrids was much better than that of Japonica type. The dry matter weight of excised root in Indica- Japonica hybrids was significantly heavier than in Japonica type at both 27 and 30$^{\circ}C$ incubators. The difference of dry root weight between Japonica type and Indica- Japonica hybrids was caused from the number of lateral roots, especially the root numbers of 25 cm length. The main and lateral seminal root growth of Japonica type grew best at the lot of 0.2 percent casamino acid. However, the lots of 0 and 0.2 percent casamino acid showed good results in the main and lateral excised root growth. respectively of Indica-Japonica hydrids. The growth of excised roots was better in Indica-Japonica hybrids than in Japonica type at even various levels of pH, sucrose and casamino acid. Between the parents of Indica - Japonica hybrids, the excised root growth of Indica type was more predominant than that of Japonica type, and the effect of casamino acid on the excised root growth in the formar was similar to that of Indica type. This result indicated that the growth characteristics of excised seminal roots in Indica Japonica hybrids were derived from the parent, Indica type.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1339-1348
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• 2015

Until now, considerable effort has been made to engineer novel nitrogen-fixing organisms through the transfer of nif genes from various diazotrophs to non-nitrogen fixers; however, regulatory coupling of the heterologous nif genes with the regulatory system of the new host is still not well understood. In this work, a 49 kb nitrogen fixation island from P. stutzeri A1501 was transferred into E. coli using a novel and efficient transformation strategy, and a series of recombinant nitrogen-fixing E. coli strains were obtained. We found that the nitrogenase activity of the recombinant E. coli strain EN-01, similar to the parent strain P. stutzeri A1501, was dependent on external ammonia concentration, oxygen tension, and temperature. We further found that there existed a regulatory coupling between the E. coli general nitrogen regulatory system and the heterologous P. stutzeri nif island in the recombinant E. coli strain. We also provided evidence that the E. coli general nitrogen regulator GlnG protein was involved in the activation of the nif-specific regulator NifA via a direct interaction with the NifA promoter. To the best of our knowledge, this work plays a groundbreaking role in increasing understanding of the regulatory coupling of the heterologous nitrogen fixation system with the regulatory system of the recipient host. Furthermore, it will shed light on the structure and functional integrity of the nif island and will be useful for the construction of novel and more robust nitrogen-fixing organisms through biosynthetic engineering.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1701-1710
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• 2017

Classical swine fever virus (CSFV) is the etiologic agent of classical swine fever, a highly contagious disease that causes significant economic losses to the swine industry. The lapinized C-strain, a widely used vaccine strain against CSFV, has low growth efficiency in cell culture, which limits the productivity in the vaccine industry. In this study, a recombinant virus derived from C-strain was constructed and subjected to continuous passaging in PK-15 cells with the goal of acquiring a high progeny virus yield. A cell-adapted virus variant, RecCpp80, had nearly 1,000-fold higher titer than its parent C-strain but lost the ability to induce fever in rabbits. Sequence analysis of cell-adapted RecC variants indicated that at least six nucleotide changes were fixed in RecCpp80. Further adaption of RecCpp80 variant in swine testicle cells led to a higher virus yield without additional mutations. Introduction of each of these residues into the wild-type RecC backbone showed that one mutation, M979R (T3310G), located in the C-terminal region of E2 might be closely related to the cell-adapted phenotype. Rabbit inoculation revealed that $RecCpp40_{+10}$ failed to induce fever in rabbits, whereas $RecCpp80_{+10}$ caused a fever response similar to the commercial C-strain vaccine. In conclusion, the C-strain can be adapted to cell culture by introducing specific mutations in its E2 protein. The mutations in RecCpp80 that led to the loss of fever response in rabbits require further investigation. Continuous passaging of the C-strain-based recombinant viruses in PK-15 cells could enhance its in vitro adaption. The non-synonymous mutations at 3310 and 3531 might play major roles in the enhanced capacity of general virus reproduction. Such findings may help design a modified C-strain for improved productivity of commercial vaccines at reduced production cost.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.8
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• pp.231-239
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• 2017

This study was conducted to provide basic data about the depression of parents with school-aged children by identifying the actor and partner effects of family interaction and self-esteem on depression among parents with school-aged children. Data on 1,475 pairs from the Wave 7 Panel Study on Korean Children were analyzed by using the SPSS 18.0 program and the AMOS 18.0 program. From the analysis of a hypothetical model, parents' family interaction exerted an actor effect on self-esteem. Mother's family interaction and self-esteem exerted an actor effect on depression; however, the partner effect on father's depression was not statistically significant. Father's family interaction and self-esteem exerted an actor effect on depression; however, the partner effect of father's family interaction on mother's depression was not statistically significant, and only father's self-esteem exerted a partner effect on mother's depression. The study results are as follows. First, the affecting factors of depression in school-aged parents, which are directly related to the depression of the child, were identified and the basic data of nursing intervention for the parents'. It is expected that clinical nurses will be used as data to convey professional knowledge to control parents' depression.

• Applied Biological Chemistry
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• v.38 no.6
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• pp.507-511
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• 1995

In an attempt to improve the productivity of ${\beta}-galactosidase$ from Bacillus sp. A1, which was isolated from soil and has remarkably higher transgalactosylation activity than lactose hydrolysis activity, a chemical mutation procedure using N-methyl-N'-nitro-N-nitrosoguanidine followed by selection was conducted. The final selection, designated as Bacillus sp. A4442, turned out to show a substantially increased enzyme productivity. Catabolite repression by glucose and lactose requirement as an inducer for the enzyme biosynthesis, which were shown in the parent strain, was markedly diminished; instead it was found out that galactose acts as another inducer. Because pH of medium, one of the most important factors for cell growth as well as enzyme production, is closely related with the sugar concentration during culture, it was kept in the optimum range of $6.5{\sim}7.5$; for this the initial glucose concentration was adjusted to be 0.5% which was thereafter maintained by the controlled pumping-in of lactose using the pH-stat technique. By doing so, we were able to increase the productivity of ${\beta}-galactosidase$ with high transgalactosylation activity up to $44\;unit/m{\ell}-broth$.

• The Plant Pathology Journal
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• v.26 no.4
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• pp.313-320
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• 2010

Genetic instability of the rice blast fungus Magnaporthe oryzae has been suggested as a major factor underlying the rapid breakdown of host resistance in the field. However, little information is available on the mechanism of genetic instability. In this study, we assessed the stability of repetitive DNA elements and several key phenotypic traits important for pathogenesis after serially transferring two isolates though rice plants and an artificial medium. Using isolate 70-15, we obtained a total of 176 single-spore isolates from 10 successive rounds of culturing on artificial medium. Another 20 isolates were obtained from germ tubes formed at the basal and apical cells of 10 three-celled conidia. Additionally, 60 isolates were obtained from isolate KJ201 after serial transfers through rice plants and an artificial medium. No apparent differences in phenotypes, including mycelial growth, conidial morphologies, conidiation, conidial germination, appressorium formation, and virulence, or in DNA fingerprints using MGR586, MAGGY, Pot2, LINE, MG-SINE and PWL2 as probes were observed among isolates from the same parent isolate. Southern hybridization and sequence analysis of two avirulence genes, AVR-Pita1 and AVR-Pikm, showed that both genes were also maintained stably during 10 successive generations on medium and plants. However, one reversible loss of restriction fragments was found in the telomere-linked helicase gene (TLH1) family, suggesting some telomere regions may be more unstable than the rest of the genome. Taken together, our results suggest that phenotype and genotype of M. oryzae isolates do not noticeably change, at least up to 10 successive generations on a cultural medium and in host plants.