• 스마트미디어저널
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• 제12권10호
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• pp.71-84
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• 2023

넙치 양식은 우리나라 양식 산업의 절반 이상 차지할 정도로 큰 비중을 차지한다. 그러나 연중 총사육량의 25~30% 정도가 질병으로 인한 집단 폐사가 발생하여 양식장의 경제성에 매우 나쁜 영향을 준다. 넙치 양식장의 경제성 성장을 위해서는 넙치 질병 증상 진단을 자동화하여 빠르고 정확하게 질병 진단을 하는 방법이 필요하다. 본 연구에서는 독창적인 학습 데이터 수집 방법과 학습 정제 알고리즘 및 학습 데이터 분리 기법을 사용하여 학습 데이터를 구축하고 4가지 객체 탐지 딥러닝 모델(YOLOv8, Swin, Vitdet, MvitV2)의 넙치 질병증상 감지 성능을 비교한다. 실험 결과 YOLOv8 모델이 평균 인식률(mAP)과 예상 도착 시간(ETA) 관점에서 우수하다는 결론을 얻었다. 본 연구에서 제안하는 AI 모델의 성능이 검증되면 넙치 양식장에서는 실시간으로 넙치 질병을 진단할 수 있고, 진단 결과에 따른 신속한 예방 조치로 양식장의 생산성은 크게 향상될 것이라 기대된다.

• 한국전자통신학회논문지
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• 제18권6호
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• pp.1331-1336
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• 2023

머신러닝을 이용한 텍스트 기반 어류 질병 분류에서 머신러닝 모델의 입력 파라미터가 너무 많은 문제가 존재하지만, 성능의 문제로 임의로 입력 파라미터를 줄일 수 없다. 본 논문에서는 이 문제를 해결하고자 SHAP 분석 기법을 활용해 넙치 질병 분류에 특화된 입력 파라미터 최적화 방안을 제시한다. 제안한 방법은 SHAP 분석 기법을 적용하여 넙치 질병 문진표에서 추출한 질병 정보의 데이터 전처리와 AutoML을 활용한 머신러닝 모델 평가 과정을 포함한다. 이를 통해 AutoML의 입력 파라미터의 성능을 평가하고, 최적의 입력 파라미터 조합을 도출한다. 본 연구에서 제안 방법은 필요한 입력 파라미터 수를 감소시키면서도 기존의 성능을 유지할 수 있을 것으로 기대되며, 이는 텍스트 기반 넙치 질병 분류의 효율성 및 실용성을 높이는 데 기여할 것이다.

• 한국어병학회지
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• 제23권3호
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• pp.379-388
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• 2010

This paper presents a computer program for easy and rapid disease diagnosis of olive flounder diseases. To design the program, standard diagnosis process of the 14 olive flounder diseases was first setup, then implemented four-steps diagnosis program. To run program, first input fundamental information such as water temperature, size of the diseased fish. Then sequentially, three categories of key factors for disease diagnosis which include external clinical signs, internal clinical signs and microscopic observations are selected. When a user selects the observed signs of olive flounder from the listed options, the program provides maximum 5 presumed disease candidates in order. The disease information, treatment and prevention methods are provided by connected web server through internet. The program would support fish doctors and farmers by providing easy and rapid diagnosis of diseased olive flounder.

• 한국어병학회지
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• 제15권1호
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• pp.1-7
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• 2002

On April of 2000 and February of 2001, a disease characterized by darkening, ascites and enlargement of the spleen occurred in flounder, Paralichthys olivaceus, farmed near northern and eastern coasts of Korea. Various degree of necrosis including liquefactive-type was observed mainly in hemopoietic tissue of spleen and kindney. Certain tissue such as branchial soft tissue including epithelium showed a similar necrotic findings. But some tissues such as intentine had several necrotic clusters of wandering cells, being possibly leukocytes. Necrotic evidences of blood cells were evident in various tissues including hemopoietic tissues. Necrobiotic bodies were frequently encountered in blood smear preparations. Bullet type-virious were detected in the spleenic cells. Each virion was about 500 nm in diameter and 170nm in length. From these results, this disease was thought to be a rhabdoviral disease.

• 한국수산과학회지
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• 제49권2호
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• pp.176-183
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• 2016

In this study, disease surveillance was performed to monitor the prevalence of viral haemorrhagic septicaemia virus (VHSV) and red seabream iridovirus (RSIV) in olive flounder, Paralichthys olivaceus in 2015. The fish samples were collected in March (60 farms), May (55 farms) and July (52 farms) from different farms in Jeju. Reverse transcription polymerase chain reaction (RT-PCR) (VHSV) or PCR (RSIV) results showed that VHSV detected in 2 farms, but RSIV has not been detected in any farms. The sequences of the nucleocapsid protein (N) and glycoprotein (G) gene of the 2 VHSV isolates were successfully sequenced. Phylogenetic analysis was included VHSV isolates reported here together with a representative VHSV isolates available in GenBank. Phylogenetic analysis indicated that most of Korea VHSV isolates were closely related to the Japan and China genotype IVa which is clearly distinct from the North American genotype IVb.

• 한국어병학회지
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• 제26권1호
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• pp.45-50
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• 2013

Enrofloxacin is one of the normally used flouroquinolones in mammalian and fish but its withdrawal time and studies were remain obscure. The residual contents of enrofloxacin in fish muscle were analyzed by using HPLC-FLD. More than 0.1 mg/kg of ENR was detected in muscle tissues and the residues were found over 1 year after treatment. The concentration of ENR in Paralichthys olivaceus was not affected by water temperature and lasted for an extended amount of time. The spike recoveries of ENR in the muscle tissue ranged from 78% to 85%. From this results, we need the prescription by veterinarian or aquatic organism disease inspector in ENR usage to assure safety of fish. Future research is required to determine the recommendation dose of ENR for side effects and safety.

• 한국수산과학회지
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• 제54권5호
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• pp.634-643
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• 2021

Han In-jin (Artemisia iwayomogi Kitamura) and Cham Dang-gwi (Angelica gigas Nakai) exhibit antibacterial, antiparasitic, antifungal, and antiviral properties in vitro. In this study, mixture of the extracts of these two medicinal plants was absorbed on pellets. Thereafter, these pellets were fed to olive flounder Paralichthys olivaceus for 12 weeks at laboratory (1^st experiment) and 24 weeks at field test (2^nd experiment), and the immune activity and disease resistance properties of the extracts were examined. It was observed that lysozyme activities of plasma, spleen, and kidney improved after 12 weeks. Furthermore, when the olive flounders were artificially infected with bacterial pathogens, their cumulative mortality decreased in the group that was fed the extracts for 12 weeks compared to that in control group, and the relative percent survival also improved. This study concluded that mixture of Han In-jin and Cham Dang-gwi extracts provides disease resistance in vivo.

• 한국어병학회지
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• 제6권2호
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• pp.219-231
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• 1993

The author introduces the preventive and therapeutic methods of diseases in Japanese flounder which have been conducted by the Fish Disease Laboratory, the Mikamewan Fishery Cooperative Union, Ehime Prefecture, since 1982. Prevention 1. Addition of a sand substrate in the culture pond was effective to prevent ulcer disease. 2. Bathing in 0.5ppm of copper ion was effective to prevent some parasites. 3. Low stocking density of the fish reduced an incidence of edwardsiellosis or gliding bacterial disease. 4. Removal of the diseased fish prevented thd spread of lyphoeystis. 5. So-called $\pi$-water was effective to prevent the fry from some diseases. 6. Immersion of the juvenile in a sodium nifrusylate solution during transportation was effective to prevent gliding bacterial disease. Therapy 1. Sodium nifrustylate or oxytetracycline was effective to cure gliding bacterial disease. 2. Bathing in formalin(150ppm) or freshwater was effective to cure scuticociliatidosis. 3. Erythromycin was effective to cure $\beta$-hemolytic Streptococcus sp. infection. 4. Bathing in a hydrogen peroxide solution(1.5%) was effective to cure white spot disease.

• 한국수산과학회지
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• 제45권6호
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• pp.666-674
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• 2012

We examined the cause of a disease outbreak in adult olive flounder Paralichthys olivaceus, which occurred at a Korean aquaculture farm in Korea in 2011. The principal signs included an expanded abdomen and congested liver, with persistent mortality (a little over two months). At the beginning of the outbreak, farm administrators misjudged the disease as bacterial in origin, because of the aforementioned signs, persistent mortality, and the detection of bacterial species, including Vibrio spp. and Streptococcus spp. Moreover, the detection of viral hemorrhagic septicemia virus (VHSV) by reverse trasnscription-PCR analysis was complicated by use of the VHS-VN primer set, which has been in general use recently, because it produced weak bands in some samples. Therefore, we recommend the use of at least two different primer sets in the diagnosis of VHSV. Our histopathological findings indicate that necrotizing myocarditis could be considered a pathogenic sign of VHSV infection.

• 한국수산과학회지
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• 제40권3호
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• pp.128-132
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• 2007

We identified a microsatellite marker, Poli121TUF, which appears to be significantly linked (P<0.001) with a lymphocystis disease virus (LCDV)-resistance gene in the olive flounder, Paralichthys olivaceus. The olive flounder is an economically important food fish, that is widely cultured in Korea, Japan, and China. Lymphocystis disease has spread in these countries and has seriously reduced the economic value of the fish. LCDV causes lymphocystis cells (LC) to form on the body surface, fins, gills, mouth, and intestine. Fish with LC lose commercial value due to their deformed appearance. The identified micro satellite marker can be used as a candidate locus for marker-assisted selection (MAS) in order to enhance the efficiency of selection for LCDV resistance in the olive flounder.