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http://dx.doi.org/10.5657/KFAS.2012.0666

Diagnosis Case of Viral Hemorrhagic Septicemia (VHS) in Adult Olive Flounder Paralichthys olivaceus  

Kim, In-Woo (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute)
Cho, Mi Young (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute)
Lee, Han-Na (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute)
Han, Hyun Ja (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute)
Oh, Yun Kyeong (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute)
Lee, Soon Jeong (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute)
Jee, Bo Young (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute)
Myeong, Jeong-In (Southwest Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
Won, Kyoung-Mi (Southwest Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.45, no.6, 2012 , pp. 666-674 More about this Journal
Abstract
We examined the cause of a disease outbreak in adult olive flounder Paralichthys olivaceus, which occurred at a Korean aquaculture farm in Korea in 2011. The principal signs included an expanded abdomen and congested liver, with persistent mortality (a little over two months). At the beginning of the outbreak, farm administrators misjudged the disease as bacterial in origin, because of the aforementioned signs, persistent mortality, and the detection of bacterial species, including Vibrio spp. and Streptococcus spp. Moreover, the detection of viral hemorrhagic septicemia virus (VHSV) by reverse trasnscription-PCR analysis was complicated by use of the VHS-VN primer set, which has been in general use recently, because it produced weak bands in some samples. Therefore, we recommend the use of at least two different primer sets in the diagnosis of VHSV. Our histopathological findings indicate that necrotizing myocarditis could be considered a pathogenic sign of VHSV infection.
Keywords
Viral hemorrhagic septicemia (VHS); Reverse transcription polymerase chain reaction (RT-PCR); VHS-VN primer; Diagnosis; Necrotizing myocarditis;
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Times Cited By KSCI : 3  (Citation Analysis)
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