• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.174-179
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• 2006

A cDNA library was constructed from leaf samples of 4-year-old Panax ginseng cultured in a field. 3,000 EST from a size selected leaf cDNA library were analyzed. The 349 of 2,896 cDNA clones has related with energy metabolism genes. The 349 known genes were categorized into nine groups according to their functional classification, aerobic respiration(48.4%), accessory proteins of electron transport and membrane associated energy conservation(17.2%), glycolysis and gluconeogenesis(3.4%), electron transport and membrane associated energy conservation(2.9%), respiration(2.0%), glycolysis methylglyoxal bypass(1.7%), metabolism of energy reserves(0.6%) and alcohol fermentation(0.3%).

• Journal of Ginseng Research
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• v.20 no.1
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• pp.72-77
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• 1996

This study was conducted to establish an identification method age for cultivated ginseng (Panax ginseng C.A. Meyer) based on counting the stem vestige at rhizome. Weight, diameters of body and lateral roots, and ratios of those diameters were overlapped with those of other ages, so these are not enough as a criterion for identification of ginseng age. Since the stem vestige at rhizome was remained clearly from the 3rd-year age, root age can be the number of stem vestige +2. The number of stem vestige should be counted at the one side of rhizome with abundant vestige for multi stewed plant. It is necessary that the number of year-ring in root is investigated for rhizome damaged plant, but it must be considered that the new year-ring can be shown clearly after around loth of June. All dormant roots had damaged rhizome by some reasons.

• Proceedings of the Ginseng society Conference
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• 1990.06a
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• pp.79-85
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• 1990

The aqueous extract of fresh ginseng (Panax ginseng C.A. Meyer) contains a macromolecular fraction that showed mitogenic and comitogenic activities in human peripheral blood Iymphocytes. Purification of the crude extract by size (ultrafiltration, Sephadex G-200) and charge (DEAE-cellulose. DEAE-Sepharose) yielded a semi-purified fraction (DS-3). This fraction contains at least three subgroups of anionic macromolecules with apparent molecular weight greater than 600 kilodaltons. It is a glycoprotein with a large amount of glucuronic acid. It acts as a mitogen in both T and B cells of human peripheral blood lymphocytes. It could also potentiate the mitogenic action of Concanavalin A in Iymphocyte T cells. Such potentiation is not due to increased binding of Concanavalin A to the cell surface. Its mitogenic and co-mitogenic effects do depend on the presence of extracellular Ca2+.

• Journal of Ginseng Research
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• v.8 no.2
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• pp.124-134
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• 1984

Ginseng root starch, prepared by conventional method, contained crude lipid of 0.5%, crude protein of 0.4%, crude mineral of 0.17% and phosphorous of 12.5mg% as noncarbohydrate constituents. The amylose content of ginseng root starch picked in Summer (May to August) and Winter (November to March) was 32-35% and 15-20%, respectively, and it was decreased with a growing preiod of ginseng. The blue value, alkali number and ferricyanide number of the starch were 0.14-0.17, 8.50 and 0.781, respectively. The molecular weight of amylose in the starch was estimated to be 1.27-7.95${\times}$105 by means of periodate oxidation, and the degree of branching and glucose unit per segment of amylopectin were 3.50-3.53% and 28.3-28.5, respectively, The starch content of ginseng root was decreased when dried under sunlight and stored at 5$^{\circ}C$ for twenty days. In contrast, sucrose content in the root was increased from 3.8% in fresh state to 11.5% during storage at the above condition. In the other hand, starch was converted to maltose by heating at temperature above 70$^{\circ}C$.

• Journal of Ginseng Research
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• v.9 no.2
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• pp.264-269
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• 1985

The inheritance of violet and green stems was examined in F1 F2 of violetstem variant x yellow-berry variant in Panax ginseng C. A. Meyer, and the characters of F1 and F2 plants were investigated. From these results, it was shown that most of the characters of F1 and F2 plants were simillar to the female plants. However, reciprocal crosses between violet and green stems yielded progeny of violet-stem variant. Thus the cross V♀x♂Y gave all violet-stem seedlings, and the cross Y♂ x Y♀ gave all violet-stem seedlings. And all of the crosses segregated in F2 in a ratio of 3 violet to 1 green.

• Journal of Ginseng Research
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• v.15 no.2
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• pp.139-143
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• 1991

This study investigated the relations between the photosynthetic rate and the activities of antioxidatile enzymes, glutathione reductase, ascorbate free radical reductase, ascorbate peroxidase, glutathione peroxidase, and ascorbate oxidase, in the leaves of Panax ginseng. Under the normal cultivation condition, Panax in showed lower g1utathione reductase and ascorbate free radical reductase activities the Glycine max. But P ginseng showed higher 91utathione Peroxidase, ascorbate Peroxidase, and ascorbate oxidase activities than C. Panax. Therefore, P. ginseng showed weak activities of reductases for the reduction of the oxidized antioxidants. Under the light intensity of 25 KLux, the reductases showed a decrease of over 75% after 24 hours. But the peroyoxidases decreased about 40%. These results showed that the decrease of reductases acitivities was consistent with the decrease of photosynthetic rate. Therefore, we consider that the regulation of antioxidative enzymes or the application of exogenous antioxidants will be effective means for the protection of photodamage in p. ginseng.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.242-247
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• 1989

The effect of ginseng extract and sodium ascorbate (vitamin C) administered separately or in combination on the some cancer cells cultured in vitro have been examined. Mouse leukemic cells (L1210 and P388), human rectal cancer cells (HRT-18) and human colon cancer cells (HCT-48) were used for the experiment. When given separately, the growth rate for each kind of cancer cell was inhibited In proportion to the concentration of ginseng extract or vitamin C. The inhibitory effect on the growth rate of the cancer cells was stronger in ginseng extract than in vitamin C except for the HCT-48 cells. Based on the cytotoxic activity, combined administration of ginseng extract and vitamin C demonstrated a synergistic inhibition of cancer cell growth. The cytotoxic activities of ginseng extract and vitamin C on the mouse leukemic cells were more sensitive than on human colon cancer cells. And the sensitivity of cytotoxic activity was somewhat different in different cancer cell lines.

• Journal of Ginseng Research
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• v.11 no.1
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• pp.17-23
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• 1987

A crude alkaloidal fraction from white ginseng (Panax.gilfsertg C.A. Meyer) showed over thirteen Dragendorff positive spots by TLC using eluent of $CHCl_3$/MeOH(10 : 1). TLC pattern of white, red and Sanchii ginseng (P. notoginseng) was mostly not different from each other, but, in American ginseng (P. quinqgtefolium),two spots having Rf 0.71 and 0.68 were not detected. An alkaloid component was isolated from white ginseng and identified as $N_9$-formyl-1-methyl-$\beta$-carboline (mp $174^{\circ}C$, $C_13H_10N_2O$).

• Journal of Ginseng Research
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• v.13 no.1
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• pp.92-97
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• 1989

This study investigated the effects of high light intensity (100 KLw) and high temperature (45 ℃, dark) on enzyme (glucose-6-phosphate dehydrogenase, acid phosphatase, catalase, peroxidase, and proteinase) activities and characteristics of Panax ginseng C.A. Meyer leaves. Enzyme activity and protein content decreased rapidly under treatment with high light intensity In P ginseng the thermal stabilities of catalase and peroxidase were high (above 70%), and the coagulation rates of soluble proteins were low (below 17%). Therefore, the decrease in enzyme activity and protein content was not caused by increase in leaf temperature due to the high light intensity, but by increase in proteolytic activities. The photochemical formation rate of superoxide radical (O-2) was higher in the P ginseng leaf extracts than in Solanum nigmm, and was accelerated by addition of crude saponin to the buffer extracts.

• Journal of Applied Biological Chemistry
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• v.62 no.4
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• pp.375-384
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• 2019

The root of Panax ginseng C.A. Meyer were extracted with 70% aqueous EtOH and the concentrates were partitioned into MeOH and H₂O fractions using Diaion HP-20. The repeated SiO₂ or octadecyl SiO₂ column, and MPLC for the MeOH fraction led to isolation of four malonyl ginsenosides. The chemical structures of these compounds were determined as malonyl ginsenoside Rd (1) malonyl ginsenoside Rc (2) malonyl ginsenoside Rb2 (3) malonyl ginsenoside Rb1 (4) based on spectroscopic analyses including Nuclear magnetic resonance and HR-TOF/MS. The contents of malonyl ginsenoside Rb1 was highist as 5.44 mg/g of five years of ginseng. And malonyl ginsenoside Rd was lowest as 0.11 mg/g of six years of ginseng. Additionally, the malonyl ginsenoside Rd exhibited hepatoprotective effect against ethanol-induced hepatotoxicity in HepG2 cell line.