Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Gene Analysis Related Energy Metabolism of Leaf Expressed Sequence Tags Database of Korean Ginseng (Panax ginseng C.A. Meyer)

고려인삼(Panax ginseng C.A, Meyer)의 잎 ESTs database에서 Energy 대사 관련 유전자 분석

  • Lee Jong-Il (School of Natural Science, Sunchon National Univ.) ;
  • Yoon Jae-Ho (School of Agriculture and Life Science, Sunchon National Univ.) ;
  • Song Won-Seob (School of Agriculture and Life Science, Sunchon National Univ.) ;
  • Lee Bum-Soo (Research Institute of Biotechnology, BioPia Co., Ltd) ;
  • In Jun-Gyo (Research Institute of Biotechnology, BioPia Co., Ltd) ;
  • Kim Eun-Jeong (Research Institute of Biotechnology, BioPia Co., Ltd) ;
  • Yang Deok-Chun (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
  • Published : 2006.02.01
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Abstract

A cDNA library was constructed from leaf samples of 4-year-old Panax ginseng cultured in a field. 3,000 EST from a size selected leaf cDNA library were analyzed. The 349 of 2,896 cDNA clones has related with energy metabolism genes. The 349 known genes were categorized into nine groups according to their functional classification, aerobic respiration(48.4%), accessory proteins of electron transport and membrane associated energy conservation(17.2%), glycolysis and gluconeogenesis(3.4%), electron transport and membrane associated energy conservation(2.9%), respiration(2.0%), glycolysis methylglyoxal bypass(1.7%), metabolism of energy reserves(0.6%) and alcohol fermentation(0.3%).

본 연구에서는 인삼 잎으로부터 정제한 mRNA를 이용하여 cDNA library를 제작하였다. 이 cDNA library로 부터 349개의 에너지 대사 관련 유전자를 선발 하였다. 에너지 대사 관련 유전자의 평균 사이즈는 0.49 kb이며, 에너지 관련 유전자들의 세부 기능별 발현을 분석한 결과 aerobic respiration(48.4%), accessory proteins of electron transport and membrane associated energy conservation(17.2%), glycolysis and gluconeogenesis(3.4%), electron transport and membrane associated energy conservation(2.9%), respiration(2.0%), glycolysis methylglyoxal byp-ass(1.7%), metabolism of energy reserves(0.6%)와 alcohol fermentation(0.3%)의 분포를 보였다. 인삼 잎에서 발현되는 유전자중 가장 많이 발현된 Chlorophyll a/b binding protein of IhcII type I(36.6%), Photosystem II oxygen-evolving complex protein(6.6%) 등이 발현되었다.

Keywords

References

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