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http://dx.doi.org/10.3839/jabc.2019.052

Identification and quantification of major malonyl ginsenosides isolated from Panax ginseng C.A. Meyer  

Shin, Woo Cheol (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Jung, Jiyun (Punggi Ginseng Research Institute, GBARES)
Na, Hyeon Seon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Bo, Jeon Hwang (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
Kim, Hyoung-Geun (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
Yoon, Dahye (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Choi, Bo-Ram (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Young-Seob (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Baek, Nam-In (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
Lee, Yi (Department of Industrial Plant Science and Technology, Chungbuk National University)
Lee, Dae Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Applied Biological Chemistry / v.62, no.4, 2019 , pp. 375-384 More about this Journal
Abstract
The root of Panax ginseng C.A. Meyer were extracted with 70% aqueous EtOH and the concentrates were partitioned into MeOH and H2O fractions using Diaion HP-20. The repeated SiO2 or octadecyl SiO2 column, and MPLC for the MeOH fraction led to isolation of four malonyl ginsenosides. The chemical structures of these compounds were determined as malonyl ginsenoside Rd (1) malonyl ginsenoside Rc (2) malonyl ginsenoside Rb2 (3) malonyl ginsenoside Rb1 (4) based on spectroscopic analyses including Nuclear magnetic resonance and HR-TOF/MS. The contents of malonyl ginsenoside Rb1 was highist as 5.44 mg/g of five years of ginseng. And malonyl ginsenoside Rd was lowest as 0.11 mg/g of six years of ginseng. Additionally, the malonyl ginsenoside Rd exhibited hepatoprotective effect against ethanol-induced hepatotoxicity in HepG2 cell line.
Keywords
Hepatoprotective activity; Malonyl ginsenoside; Nuclear magnetic resonance; Panax ginseng; Quantification; HepG2;
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Times Cited By KSCI : 1  (Citation Analysis)
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