• Journal of Ecology and Environment
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• v.41 no.9
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• pp.265-270
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• 2017

Background: Over the years, pine pollens have been excluded as an allergen due to its relatively large size, low protein content, and waxy hydrophobic layer, despite their abundance. However, recent studies suggest the possibilities of pine pollens being allergens, and it has been reported that allergy symptoms were highly prevalent in areas with considerably large pine forests and high possibility of exposure to the pollen. Therefore, we conducted a comparative analysis of the allergenicities of the pollens from the dominant species of Korean pines, red pine (Pinus densiflora), black pine (Pinus thunbergii), and pitch pine (Pinus rigida), in mice. Methods: The protein composition of the pollens from the three pine species was compared via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The pine pollens and proteins extracted from the pollens were introduced to BALB/c mice by nasal inhalation and application to exposed skin and the IgE produced by the mice were extracted from blood and analyzed via ELISA. Results: SDS-PAGE showed differing protein compositions of the pollens of the three pine species. Analysis of blood IgE compositions showed a similar amount of IgE produced when pollens were applied to skin. In contrast, when mice inhaled the pollens, P. densiflora was shown to induce significantly more IgE production than those of the other two species. Conclusions: The experimental results demonstrate that the pollens of all three South Korean pine species induce IgE production, and this production was more pronounced when the pollens were inhaled than when they were applied to the skin. Of the three species, the pollen of P. densiflora was found to induce the highest level of IgE production.

• Journal of Digital Convergence
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• v.15 no.2
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• pp.81-88
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• 2017

I conducted a study on the capacity evaluation and management of the main homepage screen to improve the availability of government institutions. As a result of three times evaluating the size of the homepage main page of the major institutions, the capacity of the homepage of the institution was very enormous, and when there were many concurrent users, the server was easily loaded. Governmental management guidelines are needed to fundamentally solve these problems, with the periodic and ongoing homepage management of each institution. In the future, the web site management detailed checkpoints should be additionally established in the government web site installation and management guidelines, and the recommended checking items; homepage performance evaluation, lightening homepage capacity.

• Journal of Life Science
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• v.25 no.4
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• pp.385-389
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• 2015

In this study, it is reported that a large polyprotein can be stoichiometrically cleaved by the use of caspase-3-dependent proteolysis. Previously, it has been shown that the proteolytic IETD motif was partially processed when treated with caspase-3, while the DEVD motif was completely cleaved. The cleavage efficiency of the DEVD-based substrate was approximately 2.0 times higher than that of the IETD substrate, in response to caspase-3. Based on this, 3 protein genes of interest were genetically linked to each other by adding two proteolytic cleavage sequences, DEVD and IETD, for caspase-3. Particularly, glutathione-S transferase (GST), maltose binding protein (MBP), and red fluorescent protein (RFP) were chosen as model proteins due to the variation in their size. The expressed polyprotein was purified by immobilized metal ion affinity chromatography (IMAC) via a hexa-histidine tag at the C-terminal end, showing 93 kDa of a chimeric GST:MBP:RFP fusion protein. In response to caspase-3, cleavage products, such as MBP:RFP (68 kDa), MBP (42 kDa), RFP (26 kDa), and GST (25 kDa), were separated from a large precursor GST:MBP:RFP (93 kDa) via SDS-PAGE. The results obtained from this study indicate that a multi-protein can be stoichiometrically produced from a large poly-protein by using proteolytic recognition motifs, such as DEVD and IETD tetra-peptides, for caspase-3.

• The KIPS Transactions:PartD
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• v.18D no.3
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• pp.205-214
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• 2011

Client based web mashups have become one of the most important architecture in web application development. Although there are well known methods to generate presentation view codes for web services, navigations between views and service requests are still developed manually in most web mashups. In this paper, we propose the concept of deterministic data binding from output data to input parameters of another method. Using binding relations, we can model navigation menus for service method requests. For a given set of data mappings between services, we investigate context dependent binding conditions and discuss the generation of views and navigation menus. The proposed approach provides UI for users to navigate services of client mashup page using simple and convenient interface even when the number of services and the size of the mashup page grows. In order to show the usability of the proposed approach, we present a historic tourism service.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.9
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• pp.617-625
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• 2009

NAND flash memory is widely used in embedded systems because of many attractive features, such as small size, light weight, low power consumption and fast access speed. However, it requires garbage collection, which includes erase operations. Erase operation is very slow. Besides, the number of the erase operations allowed to be carried out for each block is limited. The proposed garbage collection method focuses on minimizing the total number of erase operations, the deviation value of each block and the garbage collection time. NAND flash memory consists of pages of three types, such as valid pages, invalid pages and free pages. In order to achieve above goals, we use a page rate to decide when to do garbage collection and to select the target victim block. Additionally, We implement allocating method and group management method. Simulation results show that the proposed policy performs better than Greedy or CAT with the maximum rate at 82% of reduction in the deviation value of erase operation and 75% reduction in garbage collection time.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.4
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• pp.687-695
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• 2007

There has been increasing of using Internet shopping mall like an e-business, and it means that the analysis technique of appetence for webpase visitors logging into the case of analyzing the degree of concern and using them in the personalization has been absolutely advanced. For heavy web pages, it is impossible to use click-stream based analysis in analyzing interest for each area by what kind of information the visitors are interested in to. A web browser of a limited size has difficulty in expressing on a screen information about what they want, or what hey are looking for. Pagescrolling is used to overcome such a limitation in expression. In this study, a analyzing system of degree of concern for Webpage is presented, designed and implemented using page scrolling to track the position of the scroll bar and movements of the window cursor regularly within a window browser for real-time transfer to analyze user's interest by using information received from the analysis of the visual perception area of the web page.

• Anatomy and Cell Biology
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• v.56 no.3
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• pp.382-393
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• 2023

Cell clusters are a histological hallmark feature of intervertebral disc degeneration. Clusters arise from cell proliferation, are associated with replicative senescence, and remain metabolically, but their precise role in various stages of disc degeneration remain obscure. The aim of this study was therefore to investigate small, medium, and large size cell-clusters. For this purpose, human disc samples were collected from 55 subjects, aged 37-72 years, 21 patients had disc herniation, 10 had degenerated non-herniated discs, and 9 had degenerative scoliosis with spinal curvature <45°. 15 non-degenerated control discs were from cadavers. Clusters and matrix changes were investigated with histology, immunohistochemistry, and Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Data obtained were analyzed with spearman rank correlation and ANOVA. Results revealed, small and medium-sized clusters were positive for cell proliferation markers Ki-67 and proliferating cell nuclear antigen (PCNA) in control and slightly degenerated human discs, while large cell clusters were typically more abundant in severely degenerated and herniated discs. Large clusters associated with matrix fissures, proteoglycan loss, matrix metalloproteinase-1 (MMP-1), and Caspase-3. Spatial association findings were reconfirmed with SDS-PAGE that showed presence to these target markers based on its molecular weight. Controls, slightly degenerated discs showed smaller clusters, less proteoglycan loss, MMP-1, and Caspase-3. In conclusion, cell clusters in the early stages of degeneration could be indicative of repair, however sustained loading increases large cell clusters especially around microscopic fissures that accelerates inflammatory catabolism and alters cellular metabolism, thus attempted repair process initiated by cell clusters fails and is aborted at least in part via apoptosis.

• Journal of Plant Biotechnology
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• v.6 no.1
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• pp.63-67
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• 2004

A simple procedure for the extraction of the lipolytic enzyme from rice bran has been developed. High activity of lipolytic enzyme was obtained by first defatting the rice bran to remove lipid components with various extraction conditions. Then, after rove cycles of aqueous extraction, rice bran lipolytic enzyme was purified using micro- and ultrafiltration apparatus. Lipolytic enzyme activity was estimated by its hydrolytic action of tributyrin. The result indicated that the standard activity curve of butyric acid showed that the potential rice bran enzyme is a hydrolytic lipase enzyme. In addition, it showed higher lipolytic activity and specific enzyme activity with further purification by micro- and ultrafiltration. The size of rice bran lipase enzyme was identified through 15 ％ SDS-PAGE. The molecular weight of the rice bran lipase enzyme was 41 kDa.

• Transactions of the Society of Information Storage Systems
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• v.11 no.1
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• pp.1-5
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• 2015

The selective frequency analysis method is suggested in the holographic data storage system with the nyquist aperture to reduce the size of hologram. The image filter was designed with many different methods to improve the bit error rate caused by the nyquist aperture. In previous the methods of image restoration for HDS, an iteration time and a highly precise point spread function were necessary. In this paper, we describe the optical system with analytic method. Thereby, we expect our result help the researchers to design the filter.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.1161-1164
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• 2005

In this paper, we proposed the technique to estimate the life cycle of Internet content services based on the logistic regression model. In this paper, to define parameters of Internet contents estimating life cycle by logistic regression model, we used market size, traffic amount, page view and session-visit number as the parameters of Internet contents estimating life cycle by logistic regression model. In this paper, to compare the performance of our proposed scheme, we estimated life cycle for the download services of bell sound & character contents in mobile network. As a result, using our proposed logistic regression, we were able to estimate exactly the life cycle of the download services of bell sound & character contents.