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Purification and Biochemical Analysis of Rice Bran Lipase Enzyme  

Kim, Young Hee (Dept. of Bioinformatics Engineering, College of Engineering, Sangmyung University)
Publication Information
Journal of Plant Biotechnology / v.6, no.1, 2004 , pp. 63-67 More about this Journal
Abstract
A simple procedure for the extraction of the lipolytic enzyme from rice bran has been developed. High activity of lipolytic enzyme was obtained by first defatting the rice bran to remove lipid components with various extraction conditions. Then, after rove cycles of aqueous extraction, rice bran lipolytic enzyme was purified using micro- and ultrafiltration apparatus. Lipolytic enzyme activity was estimated by its hydrolytic action of tributyrin. The result indicated that the standard activity curve of butyric acid showed that the potential rice bran enzyme is a hydrolytic lipase enzyme. In addition, it showed higher lipolytic activity and specific enzyme activity with further purification by micro- and ultrafiltration. The size of rice bran lipase enzyme was identified through 15 ％ SDS-PAGE. The molecular weight of the rice bran lipase enzyme was 41 kDa.
Keywords
lipolytic enzyme; ultrafiltration; tributylin;
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Times Cited By KSCI : 1  (Citation Analysis)
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