• Journal of Korea Multimedia Society
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• v.14 no.2
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• pp.269-281
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• 2011

For the MD-TIX(multidimensional type inheritance index) that supports query processing for the type inheritance concept in XML databases, this paper presents an index tuning algorithm that enhances the performance of the XML query processing according to the query pattern. The MD-TIX uses a multidimensional index structure to support complex XML queries involving both nested elements and type inheritance hierarchies. In this index tuning algorithm, we first determine a shape of index page regions by using the query information about the user's query pattern, and then construct an optimal MD-TIX by applying a region splitting strategy that makes the shape of the page regions into the predetermined one. The performance evaluation results indicate that the proposed tuning algorithm builds an optimal MD-TIX by a given query pattern, and in the case of the three-dimensional query regions for the nested predicates of path length 2, the performance is much enhanced according to the skewed degree of the query region's shape.

• The Journal of the Korea Contents Association
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• v.14 no.9
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• pp.141-152
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• 2014

The user interfaces of electronic-book (e-book) readers in the e-book market are highly diverse, and this has led to major usability issues. In this paper, we analyze user preferences in terms of feature-setting options and the use of touchscreen actions in the six most commonly used e-book readers for smartphnoes. We identify alternatives for these features, which can enhance the usability of e-book readers, based on these user preferences. The survey results for the feature-setting options show that it is desirable to support at least eight background colors, as well as the ability to specify the color of the background icons. Adjusting the screen brightness using a setting bar with the support of an auto-brightness option is desirable, as in using +/- buttons to adjust the font size, as well as approximately 10 font faces. We find that it is desirable to support fade, slide, scroll and curl page-turing options, in addition to a simple non-animated page-turning effect, and that page movement should be accomplished using a scroll bar with the support of the page movement features by entering a page number, and by using the table of contents as well as bookmarks. The survey results on the use of touchscreen options indicated that it is useful to be able to configure the screen layout of an e-book reader by dividing it into three areas: left, right, and center. And then, when users briefly touch the left and right areas, it is ideal to move to the previous and subsequent pages, respectively; and when they briefly touch the center region, it is desirable to display a touch feature menu. We believe that the results of this study may provide guidance in the design of user interfaces for e-book readers.

• Journal of Microbiology
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• v.44 no.4
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• pp.369-374
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• 2006

To investigate the mechanism of salt tolerance of gram-positive moderately halophilic bacteria, two-dimensional gel electrophoresis (2-D PAGE) was employed to achieve high resolution maps of proteins of Halobacillus dabanensis $D-8^T$. Approximately 700 spots of proteins were identified from these 2-D PAGE maps. The majority of these proteins had molecular weights between 17.5 and 66 kDa, and most of them were distributed between the isoelectric points (pI) 4.0 and 5.9. Some protein spots were distributed in the more acidic region of the 2-D gel (pI <4.0). This pattern indicated that a number of proteins in the strain $D-8^T$ are acidic. To understand the adaptation mechanisms of moderately halophilic bacteria in response to sudden environmental changes, differential protein profiles of this strain were investigated by 2-D PAGE and $Imagemaster^{TM}$ 2D Platinum software after the cells were subjected to salt shock of 1 to 25% salinity for 5 and 50 min. Analysis showed 59 proteins with an altered level of expression as the result of the exposure to salt shock. Eighteen proteins had increased expression, S proteins were induced, and the expression of 33 proteins was down-regulated. Eight of the up-regulated proteins were identified using MALDI-TOF/MS and MASCOT, and were similar to proteins involved in signal transduction, proteins participating in energy metabolism pathways and proteins involved in stress.

• Journal of Korean Institute of Industrial Engineers
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• v.39 no.2
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• pp.119-128
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• 2013

Most internet users utilize internet portal search engines, such as Naver, Daum and Google nowadays. But since the results of internet portal search engines are based on universal criteria (e.g. search frequency by region or country), they do not consider personal interests. Namely, current search engines do not provide exact search results for homonym or polysemy because they try to serve universal users. In order to solve this problem, this research determines keyword importance and weight value for each individual search characteristics by collecting and analyzing customized keyword at external database. The customized keyword weight values are integrated with search engine results (e.g. PageRank), and the search ranks are rearranged. Using 50 web pages of Goolge search results for experiment and 6 web pages for customized keyword collection, the new customized search results are proved to be 90% match. Our personalization approach is not the way that users enter preference directly, but the way that system automatically collects and analyzes personal information and then reflects them for customized search results.

• International Journal of Contents
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• v.11 no.2
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• pp.15-19
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• 2015

In this paper, we propose an efficient document layout analysis algorithm that includes table detection. Typical methods of document layout analysis use the height and gap between words or columns. To correspond to the various styles and sizes of documents, we propose an algorithm that uses the mean value of the distance transform representing thickness and compare with components in the local area. With this algorithm, we combine a table detection algorithm using the same feature as that of the text classifier. Table candidates, separators, and big components are isolated from the image using Connected Component Analysis (CCA) and distance transform. The key idea of text classification is that the characteristics of the text parallel components that have a similar thickness and height. In order to estimate local similarity, we detect a text region using an adaptive searching window size. An improved adaptive run-length smoothing algorithm (ARLSA) was proposed to create the proper boundary of a text zone and non-text zone. Results from experiments on the ICDAR2009 page segmentation competition test set and our dataset demonstrate the superiority of our dataset through f-measure comparison with other algorithms.

• Journal of agriculture & life science
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• v.46 no.2
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• pp.129-137
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• 2012

A carboxymethyl cellulase gene, cel5B, was cloned, sequenced, and expressed in Escherichia coli. pRCS20 in E. coli was identified from metagenomic cosmid library of cow rumen for cellulase activity on a carboxymethyl cellulose agar plates. Cosmid clone (RCS20) was partially digested with Sau3AI, ligated into BamHI site of pBluescript II SK+ vector, and transformed into E. coli $DH5{\alpha}$. The insert DNA of 1.3 kb was obtained, designated cel5B, which has the activity of hydrolyzation of CMC. The cel5B gene had an open reading frame (ORF) of 1,059 bp encoding 352 amino acids with a signal peptide of 48 amino acids and the conserved region, VIYEIYNEPL, belongs to the glycosyl hydrolase family 5. The molecular mass of Cel5B protein expressed from E. coli $DH5{\alpha}$ exhibited to be about 34 kDa by CMC-SDS-PAGE. The optimal pH was 8.0, and the optimal temperature was about $50^{\circ}C$ for its enzymatic activity.

• Journal of Life Science
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• v.19 no.4
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• pp.467-471
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• 2009

The entire D-loop region of the porcine mitochondrial DNA (mtDNA) was amplified from six pig breeds (Landrace, Duroc, Large White, Korean native pig, Berkshire, and Hampshire) using a primer set designed on the basis of reported porcine mtDNA sequences. From analyses through cloning, DNA sequencing and multiple sequence alignment, an 11-bp (TAAAACACTTA) duplication was observed after known tandem repetition in the D-loop region, which promoted hetroplasmy in mtDNA. Although the existence of the 11-bp duplication has been previously reported in Duroc and Japanese native pigs, there have not been any attempts to know the characteristics of this duplication in other breeds so far. A 150 bp fragment containing the 11-duplication was amplified and typed by polyacrylamide gel electrophoresis (PAGE). All Large Whites had two duplication units and Duroc showed heteromorphic patterns, 11.2% (9/80) of the animals had the 11-bp duplication in total. On the other hand, Landrace, Berkshire, Hampshire and Korean native pigs were non-duplicated. This result showed that the 11-bp duplication could be used as a breed-specific DNA marker for distinguishing pure Landrace and Large White breeds.

• Convergence Security Journal
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• v.18 no.5_1
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• pp.113-120
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• 2018

Today, the development of information and communication technology is rapidly increasing the number of users of network-based service, and enables real-time information sharing among users on the Internet. There are various methods in the information sharing process, and information sharing based on portal service is generally used. However, the process of information sharing serves as a cause of illegal activities in order to amplify the social interest of the relevant stakeholders. Public opinion attack using macro function can distort normal public opinion, so security measures are urgent. Therefore, security measures are urgently needed. Macro attacks are generally defined as attacks in which illegal users acquire multiple IP or ID to manipulate public opinion on the content of a particular web page. In this paper, we analyze network path information based on traceback for macro attack of a specific user, and then detect multiple access of the user. This is a macro attack when the access path information for a specific web page and the user information are matched more than once. In addition, when multiple ID is accessed for a specific web page in the same region, it is not possible to distort the overall public opinion on a specific web page by analyzing the threshold count value.

• Journal of Microbiology
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• v.44 no.1
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• pp.54-63
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• 2006

Ferritin is a major eukaryotic protein and in humans is the protein of iron storage. A partial gene fragment of ferritin (255 bp) taken from the total RNA of Periserrula leucophryna, was amplified by RT-PCR using oligonucleotide primers designed from the conserved metal binding domain of eukaryotic ferritin and confirmed by DNA sequencing. Using the $^{32}P-labeled$ partial ferritin cDNA fragment, 28 different clones were obtained by the screening of the P. leucophryna cDNA library prepared in the Uni-ZAP XR vector, sequenced and characterized. The longest clone was named the PLF (Periserrula leucophryna ferritin) gene and the nucleotide and amino acid sequences of this novel gene were deposited in the GenBank databases with accession numbers DQ207752 and ABA55730, respectively. The entire cDNA of PLF clone was 1109 bp (CDS: 129-653), including a coding nucleotide sequence of 525 bp, a 5' -untranslated region of 128 bp, and a 3'-noncoding region of 456 bp. The 5'-UTR contains a putative iron responsive element (IRE) sequence. Ferritin has an open reading frame encoding a polypeptide of 174 amino acids including a hydrophobic signal peptide of 17 amino acids. The predicted molecular weights of the immature and mature ferritin were calculated to be 20.3 kDa and 18.2 kDa, respectively. The region encoding the mature ferritin was subcloned into the pT7-7 expression vector after PCR amplification using the designed primers and included the initiation and termination codons; the recombinant clones were expressed in E. coli BL21(DE3) or E. coli BL21(DE3)pLysE. SDS-PAGE and western blot analysis showed that a ferritin of approximately 18 kDa (mature form) was produced and that by iron staining in native PAGE, it is likely that the recombinant ferritin is correctly folded and assembled into a homopolymer composed of a single subunit.

• Journal of Korea Multimedia Society
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• v.6 no.2
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• pp.194-207
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• 2003

This paper presents an optimal design methodology for the multidimensional nested attribute index (MD-NAI) that uses a multidimensional index structure for indexing the nested attributes in object databases. The MD-NAI efficiently supports complex queries involving both nested attributes and class hierarchies, which are not supported by the nested attribute index using one-dimensional index structure such as $B^+$-tree. However, the performance of the MD-NAI is very degraded in some cases of user's query types. In this paper, for the performance enhancement of the MD-NAI, we first determine the optimal shape of index page region by using the query information about the nested predicates, and then construct an optimal MD NAI by applying a region splitting strategy that makes the shape of the page regions of the MD-NAI as close as possible to the predetermined optimal one. For performance evaluation, we perform extensive experiments with the MD-NAI using various types of nested predicates and object distribution. The results indicate that our proposed method builds optimal MD-NAI regardless of the query types and object distributions. When the interval ratio of a three-dimensional query region is 1:16:236, the performance of the proposed method is enhanced by as much as 5.5 times over that of the conventional method employing the cyclic splitting strategy.