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http://dx.doi.org/10.5352/JLS.2009.19.4.467

Detection of a Large White-Specific Duplication in D-loop Region of the Porcine MtDNA  

Kim, Jae-Hwan (National Institute of Animal Science, R. D. A.)
Han, Sang-Hyun (National Institute of Animal Science, R. D. A.)
Lee, Sung-Soo (National Institute of Animal Science, R. D. A.)
Ko, Moon-Suk (National Institute of Animal Science, R. D. A.)
Lee, Jung-Gyu (Division of Applied Life Science, Gyeongsang National University)
Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University)
Cho, In-Cheol (National Institute of Animal Science, R. D. A.)
Publication Information
Journal of Life Science / v.19, no.4, 2009 , pp. 467-471 More about this Journal
Abstract
The entire D-loop region of the porcine mitochondrial DNA (mtDNA) was amplified from six pig breeds (Landrace, Duroc, Large White, Korean native pig, Berkshire, and Hampshire) using a primer set designed on the basis of reported porcine mtDNA sequences. From analyses through cloning, DNA sequencing and multiple sequence alignment, an 11-bp (TAAAACACTTA) duplication was observed after known tandem repetition in the D-loop region, which promoted hetroplasmy in mtDNA. Although the existence of the 11-bp duplication has been previously reported in Duroc and Japanese native pigs, there have not been any attempts to know the characteristics of this duplication in other breeds so far. A 150 bp fragment containing the 11-duplication was amplified and typed by polyacrylamide gel electrophoresis (PAGE). All Large Whites had two duplication units and Duroc showed heteromorphic patterns, 11.2% (9/80) of the animals had the 11-bp duplication in total. On the other hand, Landrace, Berkshire, Hampshire and Korean native pigs were non-duplicated. This result showed that the 11-bp duplication could be used as a breed-specific DNA marker for distinguishing pure Landrace and Large White breeds.
Keywords
mtDNA D-loop; Large White; 11-bp duplication; breed-specific DNA marker;
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